113 results about "Epithelial Stem Cell" patented technology

The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising the epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of the culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in the culture medium.

The present invention relates to methods of modulating epithelial stem cell lineage by regulating the expression of Lef1 or a BMP inhibitor and / or the stability of β-catenin or the expression of a Wnt; regulating the expression or activity of GATA-3; or regulating BMPR1A activity either at the level of receptor expression or at the level of pathway activation. Methods of regulating E-cadherin, GATA-3, BMPR1A and HK1-hair keratin and methods of identifying agents which modulate the epithelial stem cell lineage are further provided. Such agents are useful for inhibiting or stimulating inner root sheath development or hair follicle formation.

Transplantation of epithelial stem cells, cultured ex vivo on specifically treated amniotic membrane, yields, with that amniotic membrane, a surgical graft having expanded epithelial stem cells. The method of creating this graft and the graft itself are simple and effective to reconstruct damaged tissue, a preferred example being corneal tissue. The source of the epithelial stem cells can be a very small explant from healthy autologous and allogeneic tissue biopsy. The amniotic membrane is treated such that its extracellular matrix is maintained, but its cells are killed.

Transplantation of epithelial stem cells, cultured ex vivo on specifically treated amniotic membrane, yields, with that amniotic membrane, a surgical graft having expanded epithelial stem cells. The method of creating this graft and the graft itself provide simple and effective means to reconstruct damaged tissue, a preferred example being corneal tissue. The source of the epithelial stem cells can be a very small explant from healthy autologous and allogeneic tissue biopsy. The amniotic membrane is treated such that its extracellular matrix is maintained, but its cells are killed.

The invention provides serum-free culture fluid for culture of salivary gland epidermal cells and salivary gland stem cells of mammals, which is prepared by using MCDB153HAA as basic culture medium and adding amino acid, vitamins, salts, lipid, trace elements, buffer fluid, hormone-like compounds, transgenic metalloprotein, antioxidants, seralbumin, glucide, purine, pyrimidine base substances and pH value indicators. The serum-free culture fluid has the advantages that salivary gland epidermal cells of the mammals can vigorously grow, good cell activity and physiological properties are maintained, in addition, the serum-free culture fluid is also suitable for the salivary gland epidermal cell culture, is particularly suitable for the field of study relevant to biological tissue engineering and belongs to the commercial cell culture fluid.

Described are cell culture solutions and systems for epithelial stem cell and organoid cultures, formation of epithelial constructs and uses of the same in transplantation. A single layer of epithelial cells that actively self-renews and is organized into crypts and villi clothes the intestine. It has been recently shown that the renewal of intestinal epithelium is driven by Lgr5+ intestinal stem cells (ISC) that reside at the base of these crypts (Barker et al., 2007). Lgr5+ stem cells can be isolated and cultured in vitro to form organoids containing crypt-vcllus structures that recapitulates the native intestinal epithelium (Sato et al., 2009).

Described are cell culture solutions and systems for epithelial stem cell and organoid cultures, formation of epithelial constructs and uses of the same in transplantation. A single layer of epithelial cells that actively self-renews and is organized into crypts and villi clothes the intestine. It has been recently shown that the renewal of intestinal epithelium is driven by Lgr5+ intestinal stem cells (ISC) that reside at the base of these crypts (Barker et al., 2007). Lgr5+ stem cells can be isolated and cultured in vitro to form organoids containing crypt-vcllus structures that recapitulates the native intestinal epithelium (Sato et al., 2009).

A composite skin material prepared by tissue engineering for skin implantation is composed of the hypoderminal cell layer and epithelial cell layer attached on fibrin scaffold. Its preparing process includes such steps as taking fibroblasts and epithelial stem cells from patient itself and foreign person, dispersing them in fibrinogen liquid, preparing serozyme solution, mixing them together, and coating the mixture on the surface of wound.

Described are cell culture solutions and systems for epithelial stem cell and organoid cultures, formation of epithelial constructs and uses of the same in transplantation.

Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and / or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.

The invention relates to a serum-free medium of a human amniotic epithelial stem cell and a culture method thereof. The serum-free medium is formed by adding a human epidermal growth factor, human transferring, human insulin, sodium selenite, alanyl-L-glutamine dipeptide, alanine, asparaginate, aspartic acid, glutamic acid, glycine, proline and serine to a DMEM / F12 basic medium. The culture method comprises the steps of digesting 2.5g / L of trypsin for a human amniotic membrane, obtaining the human amniotic epithelial stem cell and filtering to prepare a single cell suspension; putting the human amniotic epithelial stem cell into a CO2 incubator of which the saturated humidity and the volume fraction are 5% at 37 DEG C through the serum-free medium under a serum-free condition and achieving growth and amplification of the amniotic epithelial stem cell through liquid exchange and subculture under the serum-free culture condition, the serum-free medium has the characteristics of stem cells, and is free of other animal sources, wide in source and free of ethical restriction.

Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and / or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.

Compositions for use in treatment of a variety of tissue diseases include stem cells and stem cell released molecules (SRM's) suspended in an aqueous solution with a cellulosic material or other thickening agent. The stem cells and SRM's can be derived from one or more distinct cell lines. The SRM's can further include one or more mucins, cytokines, or growth factors. Exemplary formulations include stem cells and SRMs derived from epithelial stem cells, corneal limbal stem cells, and fibroblasts. Other compositions and methods for formulation thereof are described.

A method of cellular therapy for a lung disease or condition in a subject is disclosed, wherein the method involves the administration of multipotent epithelial stem cells derived from amnion tissue (AECs). In a particular application, the method is used for the treatment of lung diseases and conditions such as chronic lung diseases including chronic obstructive pulmonary disease (COPD), acute lung conditions such as acute respiratory distress syndrome (ARDS), and ventilator associated lung injury (VALI).

The present invention relates to a mesenchymal stem cell-derived microparticle that has corneal epithelial stem cell and / or corneal epithelial cell growth-promoting activity, undifferentiated cornealepithelial stem cell maintenance activity, corneal epithelial stem cell colony formation-promoting activity, or a corneal epithelium protection effect.

The invention provides a culture medium for culturing mammary epithelial stem cells. The culture medium contains a TGF-beta inhibitor, B27 and / or N2, insulin, a receptor tyrosine kinase ligand, a Rock kinase inhibitor, a P38 signal transduction inhibitor, a bone morphogenetic protein inhibitor and an MST1 / 2 kinase inhibitor. The invention also provides a method for culturing cells by adopting the culture medium for the mammary epithelial stem cells, and application and methods of amplified cell populations or organoids obtained by adopting the culture method in drug screening, toxicity testing and regenerative medicine.

The present invention relates to a retinal pigment epithelial stem cell isolated from a posterior region of the retinal pigment epithelium of an adult mammal. The invention also relates to a method of inducing differentiation of retinal epithelial stem and progenitor cells in vitro, wherein the cells of the invention are highly plastic, multipotential stem cells. The invention also includes methods for the treatment of retinal diseases and vision loss involving the transplantation of retinal pigment epithelial stem cells or cells differentiated from retinal pigment epithelial stem cells to the retina of a patient in need of treatment.

The invention relates to a constructing method of a human amniotic membrane epithelial stem cell library. The method comprises the following steps: digesting a human amniotic membrane through trypsin, and carrying out filtering to prepare single-cell suspension; adding human epidermal growth factors, human transferrins, human insulin, sodium selenite, alanyl-L-glutamine dipeptide, alanine, asparaginate, aspartic acid, glutamic acid, glycine, proline and serine into a DMEM / F12 culture medium, putting cells into a CO2 incubator at temperature of 37 DEG C, saturated humidity and volume percentage of 5 percent for culturing, liquid change and passage under a serum-free condition; and putting cells obtained through in-vitro culture and amplification into liquid nitrogen for cryopreservation, and constructing a cell information file for retrieval, namely building the human amniotic membrane epithelial stem cell library. The human amniotic membrane epithelial stem cell library is used for storing human amniotic membrane epithelial stem cells and has the characteristics of wide source and no ethical restriction, and a cell culture and storage medium is animal origin-free. The human amniotic membrane epithelial stem cell library can supply the epithelial stem cells for cell treatment and other applications.

The described invention relate to systems comprising isolated human gastrointestinal segment-specific epithelial stem cell-like progenitor cells and uses thereof in drug discovery.

The invention discloses a method for preparing a cornea lamina material. The method comprises the following steps of: carrying out accellular processing on a cornea-sclera complex of a mammal eyeball, culturing an amnion epithelial stem cell, inducing and secreting TSP (Thrombospondin)-1 and co-culturing accellular cornea stroma and the amnion epithelial stem cell, sizing, packaging and sterilizing, and the like. According to an obtained cornea lamina transplanting material, the promotion and the balance among the biocompatibility, the stability, the transparency, the mechanical strength and the bioactivity are realized; the integrity of cornea collagen molecules and a lamina structure can be kept to the largest extent, and compared with that prepared with the traditional method, the accellular cornea stroma prepared according to the method disclosed by the invention is larger in tensile strength and has the moisture content and the transparency close to those of normal cornea; and various cell growth factors and the TSP-1 are gathered, and the cornea lamina material has special effects of inflammatory resistance and vascularization resistance, has the capability of greatly enhancing the bioactivity of the cornea lamina material and can be widely used for repairing complicated ocular surface coloboma such as inflammation and corneal ulcer grown in a vascularization way.

Multipotent neuroepithelial stem cells and lineage-restricted oligodendrocyte-astrocyte precursor cells are described. The neuroepithelial stem cells are capable of self-renewal and of differentiation into neurons, astrocytes, and oligodendrocytes. The oligodendrocyte-astrocyte precursor cells are derived from neuroepithelial stem cells, are capable of self-renewal, and can differentiate into oligodendrocytes and astrocytes, but not neurons. Methods of generating, isolating, and culturing such neuroepithelial stem cells and oligodendrocyte-astrocyte precursor cells are also disclosed.

A major object of the present invention is to provide a method for inducing cell differentiation into corneal epithelial stem cells and / or corneal epithelial cells, for the easy production of a corneal epithelial cell sheet having superior safety in view of the possibility of vascularization and the like occurring. A method for inducing differentiation of a pluripotent stem cell into a corneal epithelial stem cell and / or a corneal epithelial cell, the method comprising the step of culturing a pluripotent stem cell in the presence of a stromal cell or an amnion-derived factor.

Compositions and methods for generating human epithelial stem cells from induced pluripotent stem cells are disclosed. Also disclosed are methods of using cells so generated in hair transplant procedures.

The invention provides a method of manufacturing tissue engineering skin out of the body with epithelial stem cells. The method includes the following steps: (1) preparation of the epithelial stem cells: epithelial cells are made into cell suspension liquid, adhered and screened by IV collagen, and cultivated with epithelial stem cell media; (2) preparation of deepitheliarizing derma; (3) construction of the tissue engineering skin: epithelial stem cells for 2nd to 5th generation cultivation are selected, Dispase enzyme is adopted to digest the bossy cell patches in the size of rice to soya beam, the patches are inoculated to the deepitheliarizing derma, the tissue media is added, to cultivate the tissue engineering skin underwater or on the water. The tissue engineering skin constructed by the method of the invention is more suitable for the biological characteristics of human skin.

The present invention relates to the use of a plastically-compacted collagen gel as a substrate for the growth of corneal cells, particularly limbal corneal epithelial stem cells. Cells grown on such a substrate can be cultured to produce artificial ocular epithelia which can be used in ocular toxicity testing or for transplantation.