35 results about "Primary bone" patented technology

A bone fixation device may include a primary bone plate. The primary bone plate may include an elongate member having a first fastener-receiving passageway therein to receive a first fastener to anchor the primary bone plate to a long bone of a patient, and an enlarged head member extending from the elongate member and having a secondary bone plate-receiving passageway therein. The bone fixation device may also include a secondary bone plate received in the secondary bone plate-receiving passageway and having a second fastener-receiving passageway therein to receive a second fastener to anchor the secondary bone plate to fractured end portions of the long bone of the patient.

Surfaces useful for cell culture comprise a support to which is bound a CAR material, and, bound to the CAR material, collagen VI or a biologically active fragment or variant thereof and, optionally, one or more other ECM proteins (or fragments or variants thereof) such as elastin, fibronectin, vitronectin, tenascin, laminin, entactin, aggrecan, decorin, collagen I, collagen III, and collagen IV. Also, optionally present on the surface is one or more polycationic polymers, such as poly-D-lysine or poly-D-ornithine. This surface is used in cell culture to promote cell attachment, survival, and / or proliferation of a number of different cell types such as (a) liver cells (e.g., HepG2 tumor cells, and a newly discovered line of rat liver epithelial stem cells) (b) osteoblasts, such as the murine cell line MC3T3 cell line and (c) primary bone marrow cells. Kits comprising the surfaces and additional reagents are also disclosed.

Bone anchor assemblies are disclosed herein that can provide for improved fixation as compared with traditional bone anchor assemblies. An exemplary assembly can include a bracket or wing that extends down from the receiver member and accommodates one or more auxiliary bone anchors that augment the fixation of the assembly's primary bone anchor. Another exemplary assembly can include a plate that is seated between the receiver member and the rod and accommodates one or more auxiliary bone anchors that augment the fixation of the assembly's primary bone anchor. Another exemplary assembly can include a hook that extends out from the receiver member to hook onto an anatomical structure or another implant to augment the fixation of the assembly's primary bone anchor. Surgical methods using the bone anchor assemblies described herein are also disclosed.

The invention discloses a yeast hollow beta-glucan shell wrapped GMP-BSA protein targeting macrophage delivery system. The system allows BSA to be coated in a yeast shell through using a compact layer formed through the electrostatic adsorption effect of chitosan (CS), tripolyphosphate (TPP), sodium alginate and BSA. GMP-BSA particles prepared in the invention have very good protein release behavior in in-vitro experiments, and can avoid protein loss. The particles highly specifically target the macrophages, such as Raw 264.7 cells, primary BMDM cells (primary bone marrow macrophages) and peritoneal macrophages (PEMs), and are not devoured by NIH3T3, AD293, HeLa, Caco-2 and other non-macrophage tumor cells, and neutral granulocytes in blood. The macrophages play a great role in the pathogenesis of various diseases, and are very important potential target spots. The macrophages are key antigen transfer cells, and are very important in vaccine design. The system can highly selectively deliver various proteins to the macrophages in a targeting manner, and also has very wide application prospect in the field of targeting drug administration and the field of macrophage vaccine design.

Bone anchor assemblies are disclosed herein that can provide for improved fixation as compared with traditional bone anchor assemblies. An exemplary assembly can include a bracket or wing that extends down from the receiver member. The distal portion of the wing can define a bone anchor opening through which one or more auxiliary bone anchors can be disposed to augment the fixation of the assembly's primary bone anchor. A distal surface of the distal portion of the wing can be obliquely angled relative to a proximal-distal axis of the spanning portion to face one of a caudal direction or a cephalad direction. A distal surface of the distal portion of the wing can be obliquely angled relative to the proximal-distal axis of the spanning portion to face one of a medial direction or a lateral direction. Surgical methods using the bone anchor assemblies described herein are also disclosed.

The invention provides a method for separating and culturing poultry bone mesenchymal stem cells by using a bone marrow tissue block, which comprises the following steps of: after obtaining primary bone mesenchymal stem cells by a bone marrow tissue block direct wall-adherence method, carrying out in-vitro subculturing, thereby obtaining the purified poultry bone mesenchymal stem cells. Compared with the traditional method, the method has the advantages of short separation time, large obtainment and higher purity of the cells, stronger in-vitro multiplication capacity of the cells and the like and can provide abundant material sources for research application and tissue engineering of the poultry bone mesenchymal stem cells.

The invention discloses a megalobrama amblycephala bone tissue culture method. The method includes steps: 1), selecting megalobrama amblycephala of 3 months old as an experimental material; 2), taking caudal vertebra, costa and its connective tissue around of megalobrama amblycephala; 3), flushing with PBS, wetting a tissue block in fetal calf serum, and using forceps to put the tissue block at the bottom of a culture bottle; 4), putting the culture bottle into a culture box for inverted culture; 5), daily observing cell growth and replacing a culture medium; 6), performing trypsin digestion passage after cell convergence degree reaches 80% to obtain megalobrama amblycephala primary bone tissue cells; 7), using trypsin for digestion passage of the cells when convergence degree of the megalobrama amblycephala primary bone tissue cells reaches 70-80%, centrifuging before re-suspending the cells, and inoculating the cells into two new culture bottles to continue culture to obtain passage cells. The method is easy to implement and simple and convenient to operate, the problems of limitation on sample individual size and limitation on using of a trypsin digestion method are solved, and a base material is provided for related study on fish skeleton growth and development and the like in the future.

The present invention relates to the use of the protein-polymer conjugate described in the specification to treat various diseases, including primary myelofibrosis, polycythemia vera and essential thrombocythemia.

The invention provides a method and / or composition for the treatment of bone cancer including primary bone cancer and secondary bone cancer, breast cancer, skin cancer, nasopharyngeal carcinoma, oral cancer, vulva cancer, prostate cancer, cervical cancer, melanoma including melanocarcinoma by percutaneous and / or transmucosal administration of the interferon. Further, the invention provides a method and / or composition for the treatment of skin, subcutaneous, mucosal and / or submucosal primary cancer and cancer metastatic lesions by percutaneous and / or transmucosal administration of the interferon, especially a method and / or composition for the treatment of bone cancer pain including pain resulted by secondary bone cancer.

The invention discloses a human primary myelofibrosis cell line and its construction method and application. The primary myelofibrosis cell line is named human primary myelofibrosis cell line ZYXY‑M2, and was deposited in the China Center for Type Culture Collection (Wuhan University, Wuhan, China) on January 20, 2021, with the preservation number CCTCC NO :C202145. The present invention is obtained by extracting and separating mononuclear cells from the peripheral blood of patients with clinical primary myelofibrosis, and culturing them in vitro for continuous natural passage. The leukemia cell line is negative for JAK2, CALR, and MPL mutations, positive for ASXL1 mutations, positive for TP53 mutations, positive for IKZF1 mutations, positive for IDH1 mutations, positive for FLT3 mutations, and positive for TET1 mutations. With good in vitro proliferation ability, it can be used as a cell material for studying the mechanism of human primary myelofibrosis and individualized treatment in vitro. It can also be used for in vivo and in vitro research of drug screening, evaluation, and guidance for human primary myelofibrosis. Clinical medication.

The invention belongs to the technical field of regenerative medicine, and in particular relates to a sodium alginate / collagen composite bone support and a preparation method and application thereof, comprising the following steps: providing hydroxyapatite hollow microspheres loaded with antibacterial drugs; Limestone nanoparticles, collagen cross-linking agent, collagen, D-gluconolactone, sodium alginate and the antibacterial drug-loaded hydroxyapatite hollow microspheres are subjected to a pre-cross-linking reaction to obtain a pre-cross-linked mixture; The pre-crosslinking mixture is printed to obtain a primary bone scaffold; the primary bone scaffold and divalent copper ions are subjected to a re-crosslinking reaction to obtain the sodium alginate / collagen composite bone scaffold. The sodium alginate / collagen composite bone scaffold obtained by the preparation method of the present invention has good mechanical properties, relatively stable structure and antibacterial effect.

Disclosed herein are bone anchor assemblies and related methods that may provide improved fixation of a primary bone anchor. The bone anchor assembly may include a wing having a distal portion that may define a plurality of auxiliary bone anchor openings. Each auxiliary bone anchor opening may receive an auxiliary bone anchor that may enhance fixation of a primary bone anchor of the bone anchor assembly. The plurality of secondary bone anchor openings may be oriented such that when the wing is coupled to a primary bone anchor assembly of a vertebral segment in a first configuration, at least one secondary bone anchor may be driven to extend across a facet plane of the vertebral segment, and when coupled in a second configuration, at least one secondary bone anchor may be driven to extend across a facet plane of the vertebral segment. Each auxiliary bone anchor received within the wing may be driven to conform to the vertebral segment.

The present invention is for method of treating metabolic bone disease, new compounds and pharmaceutical compositions comprising the active ingredients having inhibition effects on osteoclast differentiation. The pharmaceutical composition comprising new compounds according to the present invention can be used as medicines for treating metabolic bone diseases such as bone metastatic cancer, solid cancer bone metastasis, musculoskeletal complication by solid cancer bone metastasis, hypercalcemia by malignant tumor, multiple myeloma, primary bone tumor, osteoporosis, rheumatoid arthritis, osteoarthritis, periodontal disease, inflammatory resorption of alveolar bone, inflammatory resorption of bone, and Paget's disease.

The invention discloses an umbrella bracket with main bones connected in series, and relates to the field of umbrellas. The invention solves the problems that the current umbrellas are not strong against external force and the like. A guiding main bone is arranged between the adjacent main bones of the umbrella bracket connected in series, and the upper end of the guiding main bone is hinged with the upper plate; the middle part of the main bone is provided with a fixed hinge, and the supporting bone It is hinged with the main bone through a fixed hinge; a sliding sleeve is sleeved on the guiding main bone, and the sliding sleeve and the fixed hinge are hinged through a sliding rod; the number of the main bones is 2 to 10. The utility model has the advantages of simple structure, convenient operation, easy installation and firm structure.