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Natural biomineralization powder, preparation method thereof and application thereof

A biomineralization and natural technology, applied in applications, food preparation, cosmetic preparations, etc., can solve the problems of complex preparation process, high cost, poor product quality, etc., and achieve the effect of wide source of materials and simple method

Active Publication Date: 2012-05-02
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through artificial chemical synthesis of hydroxyapatite, the preparation process is complex, the product quality is poor, the cost is high, and large-scale production is limited

Method used

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  • Natural biomineralization powder, preparation method thereof and application thereof
  • Natural biomineralization powder, preparation method thereof and application thereof
  • Natural biomineralization powder, preparation method thereof and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1 natural biomineralization powder

[0021] Buy 1kg of commercially available pork bones, wash them, remove the remaining meat on the surface of the bones and the grease and gum in the original bones, and put the processed bones into the muffle furnace. Set the furnace temperature to 800°C, start calcination and time it at the same time. During the calcination process, when the furnace temperature is about 350°C, the organic matter in the skeleton of the calcined object starts to smoke and carbonize, and when it reaches about 700°C, the calcined object in the furnace burns like charcoal fire. At 800°C, the muffle furnace automatically stops heating and starts calcination at a constant temperature. The entire calcination time is about 7 hours. Turn off the heat and allow to cool naturally. After cooling, it was taken out from the furnace, and the calcined product had turned into pure white. At the same time the hardness of the bone decreases. Finally, it...

Embodiment 2

[0029] Example 2 Application of natural biomineralized powder as a drug carrier—loaded drug lamivudine

[0030] (1) Stirring method for drug loading

[0031] The natural biomineralized powder was placed in an oven and dried at 105°C for 4 hours to constant weight. Weigh 40 mg of dried natural biomineralization powder, add 10 ml of lamivudine aqueous solution with a concentration of 3.0 mg / ml, and stir for 2 hours at 40-50° C. Centrifuge under the condition of 2000r / min for 10min, and the sedimentation is the natural biomineralized powder loaded with drug lamivudine.

[0032] Take the supernatant, dilute it 250 times, measure its absorbance at 271nm by spectrophotometry, calculate the drug loading, and the drug loading is 4.56%.

[0033] drug loading = ×100%

[0034] (2) Ultrasonic method for drug loading

[0035] Weigh 40 mg of dry natural biomineralization powder, add 10 ml of lamivudine aqueous solution with a concentration of 3.0 mg / ml, then place it ...

Embodiment 3

[0040] Example 3 Application of natural biomineralized powder as a drug carrier—loading drug 5-fluorouracil

[0041] (1) Stirring method for drug loading

[0042] Weigh 40 mg of dried natural biomineralization powder, add 10 ml of 5-fluorouracil aqueous solution with a concentration of 3.0 mg / ml, and stir at 45° C. for 2 h. Centrifuge under the condition of 2000r / min for 10min, and the precipitate is natural biomineralized powder loaded with drug 5-fluorouracil.

[0043] Take the supernatant, dilute it 250 times, measure its absorbance at 266nm by spectrophotometry, calculate the drug loading, and the drug loading is 7.12%.

[0044] (2) Ultrasonic method for drug loading

[0045] Weigh 40 mg of dry natural biomineralization powder, add 10 ml of 5-fluorouracil aqueous solution with a concentration of 3.0 mg / ml, then place it in an ultrasonicator, and conduct ultrasonication for 40 minutes at 21-22°C and 60W power. After centrifugation at 2000r / min for 10min, the precipita...

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Abstract

The invention relates to natural biomineralization powder. An adopted technical scheme is that a preparation method of the biomineralization powder comprises the following steps: cleaning animal bones, removing impurities from the surface of the bones and grease and colloid from primary bones, carrying out constant temperature calcination on the processed bones in a muffle furnace at 800DEG C for6-8h, naturally cooling, and crushing. The biomineralization powder which is prepared according to artificially and chemically synthesized hydroxyapatite in the invention has the advantages of simplemethod and wide material source. The biomineralization powder contains trace elements which are not possessed by the artificially and chemically synthesized hydroxyapatite and are possessed by human bones, and the ratio of the trace elements completely accords with organisms, so a wide prospect is opened for the biomineralization powder utilization.

Description

technical field [0001] The invention belongs to the field of inorganic biomaterials for medicine and medical auxiliary materials, in particular to natural biomineralization powder with hydroxyapatite as the main component, its preparation method and its application in pharmacy. Background technique [0002] Hydroxyapatite (Hydroxyapatite) referred to as HAP or HA, chemical formula: Ca 10 (PO 4 ) 6 (OH) 2 , accounting for 60% of the bone, it is a needle-shaped crystal with a length of 20-40nm and a thickness of 1.5-3nm, around which bone glue fibers are regularly arranged, and the crystal is a hexagonal crystal system. Hydroxyapatite has good biocompatibility and bioactivity. Its surface is fully compatible with human tissue through bonding or partially absorbed and replaced by human tissue. Therefore, it has attracted the attention of material workers and medical workers all over the world. It is a research hotspot in the field of biomaterials in recent years. Since t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K6/02A61K8/98A61K35/32A61K47/46A61L27/36A23L1/30A61P3/02A61Q11/00
Inventor 张利群郝爱军王君
Owner LIAONING UNIVERSITY
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