312 results about "Bone scaffold" patented technology

An integrated scaffold for bone tissue engineering has a tubular outer shell and a spiral scaffold made of a porous sheet. The spiral scaffold is formed such that the porous sheet defines a series of spiral coils with gaps of controlled width between the coils to provide an open geometry for enhanced cell growth. The spiral scaffold resides within the bore of the shell and is integrated with the shell to fix the geometry of the spiral scaffold. Nanofibers may be deposited on the porous sheet to enhance cell penetration into the spiral scaffold. The spiral scaffold may have alternating layers of polymer and ceramic on the porous sheet that have been built up using a layer-by-layer method. The spiral scaffold may be seeded with cells by growing a cell sheet and placing the cell sheet on the porous sheet before it is rolled.

An apparatus includes a scaffold configured to be disposed in a bone. The scaffold is configured to move from a first configuration to a second configuration. The scaffold in the second configuration is expanded from the first configuration. A selectively-expandable actuator is configured to be removably disposed within the scaffold. The selectively-expandable actuator is configured to move at least a portion of the scaffold to the second configuration when the selectively-expandable actuator is moved to an expanded configuration. A shape of the selectively-expandable actuator is substantially the same as a shape of the scaffold when the selectively-expandable actuator and the scaffold are in the second configuration. The selectively-expandable actuator configured to be removed from the scaffold when in a collapsed configuration. The scaffold is configured to remain substantially in the second configuration after the scaffold has been expanded by the actuator.

An apparatus includes a scaffold configured to be disposed in a bone. The scaffold is configured to move from a first configuration to a second configuration. The scaffold in the second configuration is expanded from the first configuration. A selectively-expandable actuator is configured to be removably disposed within the scaffold. The selectively-expandable actuator is configured to move at least a portion of the scaffold to the second configuration when the selectively-expandable actuator is moved to an expanded configuration. A shape of the selectively-expandable actuator is substantially the same as a shape of the scaffold when the selectively-expandable actuator and the scaffold are in the second configuration. The selectively-expandable actuator configured to be removed from the scaffold when in a collapsed configuration. The scaffold is configured to remain substantially in the second configuration after the scaffold has been expanded by the actuator.

Disclosed are porous ceramic balls with a hierarchical porous structure ranging in size from nanometers to micrometers, and preparation methods thereof. Self-assembly polymers and sol-gel reactions are used to prepare porous ceramic balls in which pores ranging in size from ones of nanometers to tens of micrometers are hierarchically interconnected to one another. This hierarchical porous structure ensures high specific surface areas and porosities for the porous ceramic balls. Further, the size and distribution of the pores can be simply controlled with hydrophobic solvent and reaction time. The pore formation through polymer self-assembly and sol-gel reactions can be applied to ceramic and transition metals. Porous structures based on bioceramic materials, such as bioactive glass, allow the formation of apatite therein and thus can be used as biomaterials of bioengineering, including bone fillers, bone reconstruction materials, bone scaffolds, etc.

Osteogenesis scaffold such as for spinal fusion or an intermedullary nail includes a number of arcuate struts. The scaffold may have a functional modulus of elasticity that is a result of the modulus of the material of the struts together with the architecture of the struts, and may be within the range of 5 GPa and 75 GPa. An anisotropy of a physical property such as stiffness, compressive strength or elastic modulus corresponds to the same physical property of native bone in the vicinity of the intended implantation site.

The invention relates to a pore network model (PNM)-based bionic bone scaffold constructing method. The method comprises the following steps of: acquiring a cross section image of microscopic three-dimensional micropore structural information and three-dimensional space position density information of a human bone by a micro computed tomography (Micro-CT) technology; performing threshold value processing to acquire binarized image data; extracting a spongy bone part, and measuring by using Mimics software to acquire porosity, penetration rate, aperture and the like; programming PNM bone scaffold parameters according to a PNM principle by using the acquired bone overall dimension data and internal size data; acquiring a generating program of the bone scaffold by using a programming tool C++ and OPENGRIP language programming; generating a three-dimensional model of the PNM bionic bone scaffold by using a Unigraphics (UG) secondary development platform; and finally leading the PNM bionic bone scaffold into the Mimics software to verify the parameters, such as the aperture, the penetration rate and the like of the PNM bionic bone scaffold. The bone scaffold well imitates a natural bone, and has high performance similar to that of the natural bone; and a good porous structure and the high penetration rate are favorable for differentiation and flowing of bone derived cells.

The invention relates to a regeneration bone scaffold forming system and method based on comprehensive 3D printing formation. A comprehensive 3D printing formation technology method of a regeneration bone scaffold can be built by combining an electrospinning technology and a freeze drying technology, a digital system integration method which can realize the 3D printing formation processes of the electrospinning formation and a modeling structure can be given on the basis, and finally the specific system realization method and operation steps can be given. The data processing method of the built comprehensive 3D printing formation system comprises the steps of: completing filling and lapping on each layer of the scaffold by adopting a parallel and repeated path scanning method, judging adjacent fibers through adopting a transition line method and conducting curve fitting to realize the formation of complicated contour boundaries and realize the automatic integration management of the 3D printing formation processes of the electrospinning formation and the modeling structure through specific post-processing. The regeneration bone scaffold forming system and method based on the comprehensive 3D printing formation are key technologies for realizing the multiscale formation of the regeneration bone scaffold, and have obvious characteristics.

Described herein is a novel drug delivery assembly having particular applicability to the field of orthopedic and surgical medicine. The devices and assemblies described herein enable the efficient application and retention of potentially expensive therapeutic materials to very specific locations, particularly those associated with voids in bone and tissue. One particularly unique aspect of the present invention involves the introduction of constructs which promote the retention of therapeutic material in the target area of application for beneficial use, for example by forming a proximal barrier that prevents leakage of the therapeutic material out of the target area. Additionally, the present invention provides unique devices and methods for surgical introduction of such constructs. The present invention finds particular application in connection with introduction of stem and progenitor cells, bioactive molecules and bone scaffold materials in conjunction with bone voids and with the use of cannulated implants, such as bone screws (also surgical screws) and pins. The present invention also has beneficial use in the delivery of cancer drugs, antimicrobials, bone cements and other therapeutic materials.

The invention discloses a 3D-printed gradient-diameter medical porous metal bone tissue scaffold and aims to solve the problems that a single repeated microporous structure in the prior art is adverse to bone tissue ingrowth and a bone tissue implant has difficulty in bony healing with self-bones. The scaffold is in a hexahedron structure as a whole, and comprises components A, components B and components C in tight arrangement, wherein the components A are arrayed on an outermost layer of the hexahedron structure; the components B are arrayed on a secondary outer layer; and the components C are arrayed on the innermost layer; all the components A, B and C are in a hexahedron frame structure; the pore diameter of the components A is greater than that of the components B; and the pore diameter of the components B is greater than that of the components C. Through the reinforcing structural design, the gradient-pore tissue engineering bone scaffold is produced; through regulating the ingrowth of bone tissues, fibroblasts and the like by gradient-change pores, the optimal bone healing can be achieved finally.

The invention discloses a tissue engineering bone / cartilage double-layer scaffold and a construction method and application in medical science. The method adopts natural cartilage and bone as materials, respectively removes the antigenicity of the cartilage and the bone through de-cell, and adopts a freezing and drying method to prepare the double-layer scaffold simultaneously suitable for growthof the two issues of the bone and the cartilage. A preparation method comprises that: acellular bone is soaked in suspension of cartilage acellular matrix microfilaments, and the double-layer scaffoldof which the upper layer is a cartilage acellular matrix three-dimensional porous spongy scaffold and the lower layer is an acellular bone scaffold is prepared by the freezing and drying method and acrosslinking method. The cartilage acellular matrix spongy scaffold part of the double-layer scaffold can be introduced with factors promoting the formation of the cartilage, and the acellular bone part can be introduced with factors promoting the formation of the bone, and the factors can be respectively inoculated with cells for forming the cartilage or the bone to construct a living tissue engineering bone / cartilage complex, which is used for repairing bone and cartilage complex defect or total joint defect clinically.

The present invention relates to medical biological ceramic material technology, and is a kind of artificial bone rack of nano toughened calcium phosphate of high porosity. The present invention has nano zirconia grains added into calcium phosphate to raise the compression strength and toughness of artificial bone rack, so as to provide artificial bone rack of biological ceramic material with high porosity, high compression strength and high toughness. The present invention is prepared through mixing nano zirconia grain with hydroxyapatite in certain ratio to prepare slurry, painting the slurry to polyurethane sponge with proper pore size and porosity and sintering. The present invention has high porosity, proper pore size, easy absorption, fast bone tissue fusion speed and other advantages and is used in repairing damaged bone tissue and extracorporeal cell culture.

The present invention provides a porous bioceramics for bone scaffold. The porous bioceramics according to the present invention comprises a biocompatible porous ceramic substrate having the property to thermal-decompose hydroxyapatite in contact with it; a fluorapatite (FA) inner layer formed on said porous ceramic substrate; and a hydroxyapatite (HA) outer layer formed on said fluorapatite inner layer. The insertion of FA intermediate layer can prevent the thermal reaction between ZrO2 and HA. Therefore, the present invention can provide the implant material into human body having excellent mechanical properties of zirconia as well as the biocompatibility, bioaffinity and bioactivity of HA. The present invention can also provide the implant material to promote osteoconduction and osteointegration in human body.

The invention discloses a magnesium alloy / biological ceramic bone bracket based on photocuring and gel casting and a forming method of the bone bracket. The method comprises the following steps of: establishing computer-aided design (CAD) models of the bracket and a bracket negative model through shape correlation and microstructure simulation by using reverse engineering and CAD according to the structures of different bone defect parts and the analysis results of biomechanics; making a resin bracket negative model by a photocuring technology; filling ceramic slurry into the bracket negative model by a gel casting process, curing and sintering at a high temperature to make a biological activity ceramic framework with mutually-communicated porous pipelines; and casting molten magnesium alloy into the porous pipelines of the biological activity ceramic framework by a vacuum suction casting method, cooling for solidification, and thus obtaining the magnesium alloy / biological ceramic simulation composite structure bone bracket. The internal microstructure of the made bracket consists of the mutually-communicated pipelines, the magnesium alloy is filled in the pipelines to increase the early mechanical property of the composite bracket, and the pipelines filled with the magnesium alloy become mutually-communicated pore passages as the magnesium alloy is corroded and degraded, so that the requirements of organization growth, nutrition and metabolism are met.

The invention provides a fast and reliable artificial bone prosthesis manufacturing method which includes: using the CT or MRI scanning technology to obtain the image data of all bones of a human body under a healthy state, and a building a database of the image data; generating three-dimensional models of the bones by the bone image data in the database; performing optimization design and analysis, which includes constructions of bone cavities, bone scaffolds and bone micropore basal bodies and virtual simulation analysis such as statics analysis, kinematics analysis and kinetics analysis, on the three-dimensional models of the bones; using additive manufacturing to manufacture artificial bone prosthesis on the basis of the optimized three-dimensional models of the bones; performing bioactivity treatment on the manufactured artificial bone prosthesis; performing quality inspection on the artificial bone prosthesis after the bioactivity treatment. By the method, the artificial bone prosthesis high in reliability can be manufactured fast aiming at each patient, disability possibility of the patient is lowered, and material waste is avoided.

The invention discloses a three-dimensional silk fibroin scaffold insoluble in water, and preparation and application of the three-dimensional silk fibroin scaffold. A preparation method includes the steps of injecting silk fibroin solution with the mass concentration of 0.3-30% into a mold, and obtaining a three-dimensional silk fibroin scaffold after freeze-drying; performing humid and heat crosslinking for the mold under the condition that the temperature ranges from 50 DEG C to 100 DEG C and the relative humidity ranges from 70% to 100%; and drying, removing the mold and obtaining the three-dimensional silk fibroin scaffold insoluble in water. Silk fibroin is used as a raw material, and biocompatibility is good; a preparation process is non-toxic and environment-friendly, and shape, aperture and porosity parameters of the scaffold are easy to control; the pore wall of the scaffold is nano-scale, the aperture of the scaffold is micron-scale, an extracellular matrix micro-environment is simulated, and the three-dimensional silk fibroin scaffold is used as a three-dimensional cell culture vector or a three-dimensional plasmid transfection vector when used externally; and the three-dimensional silk fibroin scaffold is used in the aspects of cartilage scaffolds, fat scaffolds, bone scaffolds, muscle scaffolds and the like when used in the internal tissue engineering field.

The invention discloses a tissue-engineered bone based on an entochondrostosis system and a construction method thereof. The construction method is characterized in that mesenchymal stem cells are cultivated on a porous bone scaffold material to construct a tissue-engineering complex, subjected to in vitro cartilage differentiation induction cultivation for two weeks and continuously conducted with fat cartilage differentiation induction cultivation for two weeks to obtain the tissue-engineered bone. According to the invention, an in vivo research result shows that the in vivo ectopic bone formation can be realized, bone defects can be successfully repaired, the difficulties that the uniform vascularization of large piece of tissue-engineered bone cannot be realized and enough nutrition can't be supplied are solved, and the construction method has a very good application prospect in the construction of the tissue-engineered bone and bone defect repairing.

The invention discloses a forming system for a tissue engineering bone and cartilage scaffold and a biological 3D printing forming method. The forming system comprises a spray head device, a three-dimensional motion mechanism, a forming table, a pressure source, a control system and a data processing system; on the basis of technical principle of pneumatic extrusion forming, motion of the three-dimensional motion mechanism and material supply of the spray head device are controlled by the control system and the data processing system, and two materials are extruded simultaneously by double parallel pin heads. According to the forming system and method for the tissue engineering bone and cartilage scaffold, control is easy, operation is simple, and a special composite graded scaffold can beprepared.

The invention discloses 3D bioprinting ink, a preparation method of the ink, a tissue engineering scaffold and a preparation method of the scaffold. The ink contains the following five components: gelatin, sodium alginate, nano-scale magnesium lithium silicate, deionized water and human mesenchymal stem cells. The preparation method of the ink comprises the following steps: firstly dissolving thesterile gelatin and the sodium alginate into the sterile deionized water in order to prepare a mixed prepolymer solution of 140-200 mg / ml gelatin and 20-60 mg / ml sodium alginate; dissolving the sterile magnesium lithium silicate into the sterile deionized water to prepare 20-60 mg / mL magnesium lithium silicate colloid; mixing the two gel in equal volume to prepare a nanocomposite hydrogel which can be used for 3D bioprinting; and finally, uniformly mixing the pre-cultured human mesenchymal stem cells and the nano-composite hydrogel to obtain the nano composite bio-ink with a final cell concentration of 3 x 10<6> / mL. The functionalized, biomimetic tissue engineering bone scaffold with osteogenesis inducing ability is prepared by using the bio-ink as a raw material and adopting a squeeze type 3D bioprinter through printing, and has potential clinical application value

Described herein is a novel drug delivery assembly having particular applicability to the field of orthopedic and surgical medicine. The devices and assemblies described herein enable the efficient application and retention of potentially expensive therapeutic materials to very specific locations, particularly those associated with voids in bone and tissue. One particularly unique aspect of the present invention involves the introduction of constructs which promote the retention of therapeutic material in the target area of application for beneficial use, for example by forming a proximal barrier that prevents leakage of the therapeutic material out of the target area. Additionally, the present invention provides unique devices and methods for surgical introduction of such constructs. The present invention finds particular application in connection with introduction of stem and progenitor cells, bioactive molecules and bone scaffold materials in conjunction with bone voids and with the use of cannulated implants, such as bone screws (also surgical screws) and pins. The present invention also has beneficial use in the delivery of cancer drugs, antimicrobials, bone cements and other therapeutic materials.

The invention discloses a high-strength degradable strontium phosphate dual-phase ceramic bone skeleton and relative preparation, wherein the initial material is calciprivia fermorite bone cement, whose solid phase powder is the mixture of Ca4(PO4)2O, SrHPO4, CaHPO4, and the liquid phase is 0.1mol / L-1mol / L H3PO4 water solution. The invention uses skeleton structure to controllably quickly print and shape RP optical sensitive resin as concave mold, to irrigate slurry, solidify, and thermally remove mold and following sinter to obtain the strontium phosphate dual-phase ceramic bone support with adjustable phases. The total porous rate of skeleton is 42.5-75%, the size of macro hole is 300mum-600mum, the volume of macro hole is 0-50%, the size of micro hole is 2-10mum, and the compression strength is 3.15MPa-21.53MPa, with wide application.

The invention provides a preparation method of a multifunctional layered joint cartilage support. The multifunctional layered joint cartilage support capable of simulating the biological property andthe mechanical property of natural cartilage is prepared by taking hydrogel as a support material, taking mesenchymal stem cells, cartilage cells and osteoblasts as inducing and developing objects andby adopting a 3D printing technology. The multifunctional layered joint cartilage support can accurately simulate the internal tissue form and the contour of each layer of the natural cartilage and can realize that different layers have different mechanical and biological characteristics. The prepared artificial cartilage support cells are derived from patients, the support serves as a degradablehydrogel material and the rejection reaction after implantation is greatly reduced. Various nutritional substances and growth factors are mixed in the hydrogel support, so that the sustained releaseeffect can be achieved and regulation and control of the growth and development of the artificial cartilage can be realized.

The invention relates to a digital coralline hydroxyapatite artificial bone scaffold and a preparation method thereof. The preparation method is characterized by comprising the following steps: 1) mixing coralline hydroxyapatite artificial bone particles and poly-L-lactic acid into a base material based on the mass ratio of 3:1; and 2) carrying out thin-layer CT scanning on bone sections, forming the shape of the artificial bone scaffold by utilizing an automatic molding machine and then carrying out laser sintering on the mixed base material to obtain the digital coralline hydroxyapatite artificial bone scaffold. The invention overcomes the complicated technology for preparing micropores in a rapid forming process and the defects that the coralline hydroxyapatite artificial bone is difficult to be prepared into the bone scaffold in a specific shape according to the clinical need; and the prepared digital coralline hydroxyapatite artificial bone scaffold can fully remain the natural micropore structure and good biocompatibility of coralline hydroxyapatite (CHA), and can be prepared into various specific shapes according to the clinical need.

The invention belongs to the technical field of the bioengineering, and particularly relates to a preparation method of a high-strength biological glass bone bracket with regular-hole distribution. The preparation method of the glass bracket with bioactivity and capability for complete degradation comprises the following steps of: using a fusion method to prepare borate bioactive glass, and crushing and sieving glass blocks into glass powder with certain size; mixing the glass powder body with organic mixed liquid propylene-glycol block polyether solution into uniform slurry; by the three-dimensional printing process of a computer, printing a bracket precursor (blank body) under a designed program; and after the blank body is dried, sintering the blank body under high temperature, and finally obtaining the glass bracket with excellent bioactivity.

The invention relates to a ligament-bone composite scaffold with a biomimetic connecting interface and a forming method thereof. The forming method comprises the following steps: firstly simulating a natural ligament-bone interface structure and utilizing a rapid forming technology to manufacture a resin negative type of a bone scaffold model with a fiber connecting structure; pouring a bone scaffold material solution into the resin negative type, and performing freeze-drying and high-temperature sintering to manufacture a bone scaffold with an internal communication pipeline and the fiber connecting structure; then primarily connecting ligament fiber with the fiber connecting structure of the bone scaffold, and fixing a die used for manufacturing of a biomimetic interface with a ligament-bone scaffold formed by primary connection; pouring the ligament material composite solution with the bone scaffold material in various changes of concentration into the interface of the ligament and the bone scaffold as secondary connection; and performing freeze-drying and removing the die, so as to obtain the ligament-bone composite scaffold with the biomimetic interface. According to the invention, the transmission of nutrients and metabolites is facilitated, the connecting strength of the ligament-bone composite scaffold is improved, and the ingrowth of cells after implantation is facilitated.

The invention relates to a method for preparing a porous bone scaffold by laser and increasing mechanical and biological performances of the porous bone scaffold by adding zinc oxide with little amount, which belongs to the bone tissue engineering field. Aiming at uncontrollable performances of aperture distribution, shape, space direction and connectivity in the preparation method of a traditional bone scaffold, and aiming at the disadvantages of poor mechanical property and fast resolving rate existed in tricalcium phosphate (beta-TCP), the invention provides the method for preparing the porous bone scaffold by using a selective laser sintering (SLS) technology, and provides the method for increasing performance by adding zinc oxide (ZnO). The method for preparing the porous bone scaffold has the advantages that the porous scaffold which enables three-dimensional interconnection is prepared by SLS, zinc oxide with little amount is added for introducing into a liquid phase, the particles densification can be promoted, crystal grain is refined, and the mechanical performance is improved, simultaneously, cell compatibility is increased, the degradation rate is reduced, and the biological performance is increased, so that the interconnected porous beta-TCP bone scaffold with enhanced mechanical performance and good biological performance can be finally prepared. The method has the advantages of simple preparation technology, convenient operation, low cost, easy control of technical parameter and the like.

The invention relates to a method for constructing a porosity-controlled bionic scaffold, which comprises the following steps of: scanning the entire natural bone by using Micro-CT technology, extracting spongy bone data and reconstructing a porous structure model of a spongy bone; measuring the porosity of the spongy bone model by using Mimics; then constructing a unit body with a proper porous structure according to the porosity; processing the unit body by using an image to obtain a three-dimensional porous structure model; and finally, performing Boolean intersection operation on the three-dimensional porous structure model and a damaged bone model so as to construct a porous structure model of the bionic scaffold, which is matched with the damaged part. In the method, the porosity corresponding to the natural bone can be obtained in the process of reconstructing and measuring, the characteristics of the natural bone can be better simulated in construction, and cell adhesion, crawling and bone replacement are more convenient. The bone scaffold constructed by the method has the same outline as real bone, which better contributes to implantation of the scaffold. A parameterized construction method can adjust different porosity characteristics of different natural bones and makes scaffold construction convenient. A construction method for obtaining the unit body by processing a unit body image solves the problem of porosity communication in a microstructure.

The invention belongs to the field of biology, and particularly relates to bone scaffold material, with a shape memory function, for jaw repair and a preparation method thereof. The bone scaffold material comprises the following raw materials in part by weight: 5 to 100 parts of polycaprolactone (PLC), 5 to 100 parts of poly butylenes succinate (PBS), 5 to 100 parts of poly (lactic-co-glycolic acid) (PLGA), and 5 to 100 parts of tertiary calcium phosphate (TCP). The bone scaffold material comprises the following raw materials in part by weight: 80 to 100 parts of polycaprolactone (PLC), 10 to 50 parts of poly butylenes succinate (PBS), 10 to 50 parts of poly (lactic-co-glycolic acid) (PLGA), and 30 to 60 parts of tertiary calcium phosphate (TCP). The method also provides a method for preparing the bone scaffold.

The invention will have a certain shape of vitreous immersed in the solution, adjusting the pH more than 7, stirring constantly, and depositing nanometer-scale hydroxyapatite on the glass surface. When dissolved all the glass, the glass in situ formed same size hydroxyapatite physical as the glass exterior. Soluble glass network is constituted oxide B2O3 or P2O5 or both, or part of both SiO2. Except CaO, glass networks gap oxides also contain one or more of the other monovalence, bivalence, tervalence, quadrivalence metal oxides, to regulate the speed of the solubility. The process is simple, easy to operate and low cost. Under the temperature of close proximity to human body, glass particles which contain calcium can combine with phosphorus in the body fluids to form inorganic mineral components, carbonic acid hydroxyapatite ceramic, which is closer to human bone tissue. Thus, this method has broad aspects of the application in the preparation of materials for hard tissue repair and bone in vitro tissue for culturing cells in bone scaffolds.

The present invention provides a porous bioceramics for bone scaffold. The porous bioceramics according to the present invention comprises a biocompatible porous ceramic substrate having the property to thermal-decompose hydroxyapatite in contact with it; a fluorapatite (FA) inner layer formed on said porous ceramic substrate; and a hydroxyapatite (HA) outer layer formed on said fluorapatite inner layer. The insertion of FA intermediate layer can prevent the thermal reaction between ZrO2 and HA. Therefore, the present invention can provide the implant material into human body having excellent mechanical properties of zirconia as well as the biocompatibility, bioaffinity and bioactivity of HA. The present invention can also provide the implant material to promote osteoconduction and osteointegration in human body.

A tissue-engineered bionic bone scaffold with high biocompatibility, bioactivity, degradability and osteoplastic induction has a three-layer structure composed of external degradable compact biofilm layer, central porous layer with less pore diameter for high mechanical strength, and internal porous layer with big pore diameter for transferring nutrients. Said central and internal layers are a composition consisting of in-situ formed nano-phase Ca-P salt and degradable biologic material.