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Forming system for tissue engineering bone and cartilage scaffold and biological 3D printing forming method

A technology of tissue engineering bone and 3D printing, which is applied in the field of molding device and preparation of osteochondral scaffold, molding device and preparation of osteochondral integrated composite scaffold, which can solve the problems of inability to regenerate osteochondral tissue, easy formation of fibrocartilage, Restore long-term functional uncertainty and other problems, achieve good transition effect, improve performance, and switch easily and quickly

Active Publication Date: 2018-03-23
SHANGHAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Clinically, the current treatment methods mainly cannot fully regenerate osteochondral tissue successfully.
All available treatments, such as revision surgery, transplantation, autologous transplantation, and autologous chondrocyte transplantation, in addition to the limitations of easy formation of fibrocartilage and lack of donor supply, are prone to further tissue damage before the therapeutic effect is achieved
However, it remains uncertain whether repairing tissue using these methods will restore long-term function

Method used

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  • Forming system for tissue engineering bone and cartilage scaffold and biological 3D printing forming method
  • Forming system for tissue engineering bone and cartilage scaffold and biological 3D printing forming method
  • Forming system for tissue engineering bone and cartilage scaffold and biological 3D printing forming method

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Experimental program
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Effect test

Embodiment 1

[0036] In this example, see Figure 1~3 , a forming system for preparing tissue-engineered osteochondral scaffolds, mainly composed of a three-dimensional motion mechanism 1, a nozzle device 2, a pressure source 3, a control and data processing system 4, and a forming platform 5; the nozzle device 2 mainly includes a barrel 8 and parallel double-needle 9; using pneumatic extrusion molding process device, the pressure source 3 communicates with the barrel 8 through the air pipe, and the barrel 8 communicates with the parallel double-needle 9 through the hose to form the extrusion pipeline for the slurry The control and data processing system 4 is respectively connected to the three-dimensional motion mechanism 1 and the pressure source 3 through the data line, the three-dimensional motion mechanism 1 pulls the forming table 5 to carry out three-dimensional movement in space, coordinates the movement of the forming table 5 and starts and stops the feeding, and the three-dimension...

Embodiment 2

[0048] This embodiment is basically the same as Embodiment 1, especially in that:

[0049] In this embodiment, a biological 3D printing forming method includes the following steps:

[0050] a. Prepare an aqueous gelatin solution with a mass percentage of 12%, add a sodium alginate solution with a mass percentage of 4% to the gelatin aqueous solution, and mix evenly according to the volume ratio of the gelatin aqueous solution and sodium alginate solution at a ratio of 1:1, and prepare Forming material hydrogel B, obtain gelatin-sodium alginate mixed solution 10ml, as cartilage layer material; Prepare the gelatin aqueous solution that mass percent is 20%, add the chitosan solution that mass percent is 5% in gelatin aqueous solution, according to gelatin The volume ratio of the aqueous solution and the chitosan solution is 1:2 and mixed evenly, and hydroxyapatite is added to enhance the mechanical strength of the prepared scaffold, and the forming material hydrogel A is prepared...

Embodiment 3

[0058] This embodiment is basically the same as the previous embodiment, and the special features are:

[0059] In this embodiment, a biological 3D printing forming method includes the following steps:

[0060] a. preparing an aqueous gelatin solution with a mass percentage of 15%, adding mTG enzyme pre-crosslinking to the gelatin aqueous solution, the mixing mass ratio of gelatin and mTG enzyme is 30:1, preparing the forming material hydrogel B according to the method, and obtaining gelatin precrosslinking Combined solution 10ml, as cartilage layer material;

[0061] Prepare an aqueous gelatin solution with a mass percentage of 20%, add a chitosan solution with a mass percentage of 5% to the gelatin aqueous solution, mix evenly according to the volume ratio of the gelatin aqueous solution and chitosan solution at a ratio of 1:2, and add hydroxyl Apatite is used to enhance the mechanical strength of the prepared bracket, and the forming material hydrogel A is prepared to obta...

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Abstract

The invention discloses a forming system for a tissue engineering bone and cartilage scaffold and a biological 3D printing forming method. The forming system comprises a spray head device, a three-dimensional motion mechanism, a forming table, a pressure source, a control system and a data processing system; on the basis of technical principle of pneumatic extrusion forming, motion of the three-dimensional motion mechanism and material supply of the spray head device are controlled by the control system and the data processing system, and two materials are extruded simultaneously by double parallel pin heads. According to the forming system and method for the tissue engineering bone and cartilage scaffold, control is easy, operation is simple, and a special composite graded scaffold can beprepared.

Description

technical field [0001] The invention relates to a molding device of an osteochondral scaffold and a preparation method thereof, in particular to a molding device of an osteochondral integrated composite scaffold and a preparation method thereof, which are applied in the technical field of biomanufacturing. Background technique [0002] Osteoarthritis is a common degenerative disease among middle-aged and elderly people all over the world, which has a great impact on people's healthy life. An estimated 40 percent of people over the age of 65 have osteoarthritis symptoms in the larger joints, severely impacting quality of life. At the same time, rising life expectancy has also increased the percentage of the population that suffers from the disease. Clinically, the current treatment methods mainly cannot fully regenerate osteochondral tissue successfully. All available treatments, such as revision surgery, transplantation, autologous transplantation, and autologous chondrocy...

Claims

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Application Information

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IPC IPC(8): A61L27/40B33Y30/00
CPCA61L27/12A61L27/20A61L27/222A61L27/3817A61L27/3821A61L27/3847A61L27/3852A61L2430/02A61L2430/06B33Y30/00
Inventor 胡庆夕吴剑平李帅谢明亮
Owner SHANGHAI UNIV
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