Preparation method of multifunctional layered joint cartilage support
A multi-functional articular cartilage technology, applied in medical science, prosthesis, additive processing, etc., can solve the problems of bionic distribution of cell density in artificial cartilage, achieve regulation, realize growth and development, and reduce rejection Effect
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Embodiment 1
[0059] This embodiment uses the preparation of rat knee joint artificial cartilage to describe a method for preparing a multifunctional layered articular cartilage scaffold, which specifically includes the following steps:
[0060] Step 1. Obtain a three-dimensional model of the cartilage tissue to be printed, and use computer processing to separate the three-dimensional model of the cartilage layer, the calcified layer, and the subchondral bone layer. The three-dimensional models of the cartilage layer, calcification layer, and subchondral bone layer were sliced and sliced separately. The layer thickness was 0.1mm. The cartilage layer was divided into N 1 The cartilage layer is divided into N 2 The subchondral bone layer is divided into N 3 Layers to obtain layered cross-sectional data.
[0061] Step 2: Culture chondrocytes in high-sugar DMEM medium to obtain a cell density of 5×10 6 cells / ml chondrocyte suspension; hypoxia (5% O 2 ) Cultivating bone marrow mesenchymal stem cel...
Embodiment 2
[0081] This embodiment uses the preparation of rabbit knee joint artificial cartilage to describe a method for preparing a multifunctional layered articular cartilage scaffold, which specifically includes the following steps:
[0082] Step 1. Obtain a three-dimensional model of the cartilage tissue to be printed, and use computer processing to separate the three-dimensional model of the cartilage layer, the calcified layer, and the subchondral bone layer. The three-dimensional models of the cartilage layer, calcification layer, and subchondral bone layer were sliced and sliced separately. The layer thickness was 0.1mm. The cartilage layer was divided into N 1 The cartilage layer is divided into N 2 The subchondral bone layer is divided into N 3 Layers to obtain layered cross-sectional data.
[0083] Step 2: Culture chondrocytes in high-sugar DMEM medium to obtain a cell density of 7×10 6 cells / ml chondrocyte suspension; hypoxia (5% O 2 ) Cultivating bone marrow mesenchymal stem ...
Embodiment 3
[0103] This embodiment uses the preparation of rabbit knee joint artificial cartilage to describe a method for preparing a multifunctional layered articular cartilage scaffold, which specifically includes the following steps:
[0104] Step 1. Obtain a three-dimensional model of the cartilage tissue to be printed, and use computer processing to separate the three-dimensional model of the cartilage layer, the calcified layer, and the subchondral bone layer. The three-dimensional models of the cartilage layer, calcification layer, and subchondral bone layer were sliced and sliced separately. The layer thickness was 0.1mm. The cartilage layer was divided into N 1 The cartilage layer is divided into N 2 The subchondral bone layer is divided into N 3 Layers to obtain layered cross-sectional data.
[0105] Step 2: Culture chondrocytes in high-sugar DMEM medium to obtain a cell density of 5×10 6 cells / ml chondrocyte suspension; hypoxia (5% O 2 ) Cultivating bone marrow mesenchymal stem ...
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