131results about How to "Reduce differentiation" patented technology

The present invention provides improved methods for producing RPE cells from human embryonic stem cells or from other human pluripotent stem cells. The invention also relates to human retinal pigmented epithelial cells derived from human embryonic stem cells or other human multipotent or pluripotent stem cells. hRPE cells derived from embryonic stem cells are molecularly distinct from adult and fetal-derived RPE cells, and are also distinct from embryonic stem cells. The hRPE cells described herein are useful for treating retinal degenerative diseases.

The present invention relates to methods of treating steroid hormone related disorders by modulating steroid hormone activity.

This invention relates, in part, to unique and newly identified genetic polynucleotides involved in the process of bone remodeling; variants and derivatives of the polynucleotides and corresponding polypeptides; uses of the polynucleotides, polypeptides, variants and derivatives; and methods and compositions for the amelioration of symptoms caused by bone remodeling disorders. Disclosed in particular are, the isolation and identification of polynucleotides, polypeptides, variants and derivatives involved in osteoclast activity, validation of the identified polynucleotides for their potential as therapeutic targets and use of the polynucleotides, polypeptides, variants and derivatives for the amelioration of disease states and research purposes.

The present invention is directed to methods and agents for modulating the differentiation potential and/or proliferation of preadipocytes. More particularly, the present invention discloses methods and agents for modulating a fibroblast growth factor (FGF) signaling pathway, especially the FGF-1 or FGF-2 signaling pathway, for treating or preventing adiposity-related conditions including, but not limited to, obesity, lipoma, lipomatosis, cachexia or lipodystrophy or the loss of adipose tissue in trauma or atrophic conditions.

The invention is concerned with a method of creating and producing examinations. The method is closely connected with a computer-based exam creation software that provides the user or instructor with a suite of tools, allowing the design of exam questions and solutions for a particular course. The system allows allow the interaction of various instructors and other participants in the examination creation process. In order to facilitate the educational process, the invention provides the user with a easy-to-use interface to create new examination questions and solutions. The system stores the questions and solutions in a database. Other professors, instructors, or others can use the system to review questions posed by other instructors so as to have peer review. The questions are labeled with various parameters. The instructor can then choose several parameters for the exam which he/she would like to create. The system will then generate an exam in the format desired for the particular class the exam is being created for. The ability of professors to peer review, share exams from a database, and have logic on how to construct the exam improves the educational process. Firstly, it increases the standardization of exams across courses and professors in university, primary or secondary schools, or elsewhere. Secondly, it adds a robustness to exams through peer review. Thirdly, if offers efficiency to professors in terms of exam production. Finally, the sharing of exam questions improves the quality of examinations for students. The system can also be used for other organizations or entities to share and distribute questions and solutions, including publishers, electronic devices, and television shows.

A backlight unit having a corner light source unit includes an optical unit having an optical sheet stacked on a bottom sash, and a liquid crystal display panel stacked on the optical sheet, and a light source unit located at the bottom sash to allow radiated light to be transferred to the liquid crystal display panel via two surfaces of the liquid crystal display panel.

The invention provides a preparation method of a multifunctional layered joint cartilage support. The multifunctional layered joint cartilage support capable of simulating the biological property andthe mechanical property of natural cartilage is prepared by taking hydrogel as a support material, taking mesenchymal stem cells, cartilage cells and osteoblasts as inducing and developing objects andby adopting a 3D printing technology. The multifunctional layered joint cartilage support can accurately simulate the internal tissue form and the contour of each layer of the natural cartilage and can realize that different layers have different mechanical and biological characteristics. The prepared artificial cartilage support cells are derived from patients, the support serves as a degradablehydrogel material and the rejection reaction after implantation is greatly reduced. Various nutritional substances and growth factors are mixed in the hydrogel support, so that the sustained releaseeffect can be achieved and regulation and control of the growth and development of the artificial cartilage can be realized.

The invention provides compositions and methods for manufacturing pluripotent cells. In particular, the invention provides improved culture platforms for manufacturing pluripotent cells with ground state pluripotency. In various embodiments, the invention contemplates, in part, a composition comprising: (a) a Wnt pathway agonist; (b) a MEK inhibitor; and (c) a ROCK inhibitor. In certain embodiments, the composition further comprises bFGF or LIF.

The present invention provides methods, media and compositions capable of modulating the differentiation of stem cells. Applicants have discovered that agonists of lysophospholipid receptors and ligands of class III tyrosine kinase receptors are useful in preventing the spontaneous differentiation of stem cells. The ligands and agonists may be used alone, or in combination where they have a synergistic effect. Also provided are cells produced using the methods and media, and methods of treating stem cell related diseases using the compositions described herein. Methods of identifying compounds useful in finding other agents useful in the modulation of stem cell differentiation are also disclosed.

The present invention relates to methods of generating and expanding hitman embryonic stem eel! derived mesenchymal-like stem/siromal cells. These hES-MSCs are characterized at least in part by the low level of expression of IL-6. These cells are useful for the prevention and treatment of T cell related autoimmune disease, especially multiple sclerosis, as well as for delivering agents across the blood-brain barrier and the blood-spinal cord barrier. Also provided is a method of selecting clinical grade hES-MSC and a method of modifying MSC to produced a MSC with specific biomarker profile. The modified MSC are useful for treatment of various diseases.

The invention discloses a culture medium for expanding human mesenchymal stem cells, which comprises a basic medium and an additive added to the medium, and the additive includes the following components at final concentrations: linoleic acid 2‑10 μL/mL, human Source AB type serum 5‑15 μL/mL, human transferrin 5‑15 μg/mL, etc. Using the culture medium to expand human mesenchymal stem cells includes the following steps: (1) isolating human mesenchymal stem cells from umbilical cord or placenta, or isolating mononuclear cells from bone marrow blood, umbilical cord blood or placental blood, and placing them in the culture medium (2) Place the cells separated and purified in step (1) into fresh medium for subculture. The invention adopts the culture medium of specific components and the expansion method, which can effectively speed up the cell expansion speed, shorten the expansion time, maintain the uniform shape of the cells after passaging, and reduce their differentiation, thereby ensuring the safety of human mesenchymal stem cells sex.

The present invention establishes the fact that the degradation of SP to SP(1-4) by endogenous NEP in BM stroma can be a mediator of hematopoietic stimulation by stem cell factor (SCF) and induce the production of TGF-β and TNF-α in BM stroma. The present invention establishes that compositions containing the SP(1-4) polypeptide, or NEP genetic elements, can be used to slow and or stop the rapid growth of stem and progenitor cells thus protecting them from the deleterious effects of cancer therapy. Hence, the polynucleotides and proteins of the present invention may be used to protect stem cells from the toxic effects of chemo- and radio-therapy, in those undergoing, or about to undergo such cancer related treatments. Also provided are compositions containing NEP antisense sequences and antibodies used for the increased proliferation and differentiation of stem and/or progenitor cells in those whom have already undergone chemo- and/or radio-therapy.

A compound coated fertilizer specially used for florists chrysanthemum is disclosed. The compound coated fertilizer is prepared from following raw materials by weight (kilogram) in parts: 9 of meerschaum powder, 1 of zinc oxide, 8 of sodium polyacrylate, 17 of sodium carboxymethylcellulose, 18 of lime powder, 18 of potassium oxide, 12 of magnesium sulfate, 1 of sodium borate, 67 of chicken manure, 46 of corn straw, 50 of cottonseed cake, 36 of castor cake, 6 of an EM bacterial agent, 7 of a soil conditioner and a proper amount of water. Fermented organic matter components and mineral components in the fertilizer can improve physical properties of the soil and activate the soil. By addition of the soil conditioner, the fertilizer has functions of enhancing fertilizer efficacy, promoting plant nutrition absorption, and slow release. By adoption of a film coating process, the fertilizer is good in slow release effect and durable in fertilizer efficiency, the number of times of pesticide using is reduced, robust growth of the chrysanthemum plant is promoted, flower bud differentiation is promoted, and blossom drop is reduced, thus increasing the output of the florists chrysanthemum.

The invention provides a method for diagnosing in a subject a condition requiring modulation of or involving trophoblast cell death, differentiation, invasion, and/or cell fusion and turnover, comprising detecting HIF Iα and the factors which modulate or are modulated by this protein, specifically TGFβ3, sFLT, VEGF, SMAD2, 3 and 7, MtdP, MtdL, MclI 1, MclIc, VHL, SiahI, Siah2, ENG, and PHD. The invention also provides a method for diagnosing or distinguishing in a subject a specific condition requiring modulation of or involving trophoblast cell death, differentiation, invasion, and/or cell fusion and turnover, in particular early onset severe preeclampsia (EPE), late onset preeclampsia (LPE) and mtre-uterme growth restriction (IUGR) comprising detecting HLF Iα and the factors which modulate or are modulated by this protein as defined above.

The invention discloses a voice endpoint detection model training method and device, and a voice endpoint detection model using method and device. The training method comprises the steps: inputting atraining audio into a generalized voice endpoint detection model; detecting a plurality of audio events existing in the training audio through the generalized voice endpoint detection model, wherein the plurality of audio events include a human speaking event, a mute event and at least one noise event; obtaining a voice and non-voice distinguishing result of the plurality of audio events output bythe generalized voice endpoint detection model; calculating a loss function based on the audio event label of the training audio and the output of the generalized voice endpoint detection model; andoptimizing the generalized voice endpoint detection model by controlling the loss function. According to the scheme of the embodiment of the invention, different types in the non-voice part can be distinguished, the classification accuracy can be improved, and the noise is not easy to misjudge as the voice part.

The invention discloses a silk ilk bracket as well as a preparation method and application thereof, and a three-phase silk ligament graft and a preparation method thereof. Firstly, silks are woven into a net-shaped bracket with a macroporous structure; then the net-shaped silk bracket is divided into three areas: A. a ligament area, B. a cartilage modification area and C. a bone modification area; ligament cells, chondrocyte and osteoblast are respectively planted in the areas A, B and C; the net-shaped bracket modified by the divided areas is rolled to form a cylindrical ligament graft with a physiological transition structure. The structure gradually transited from soft tissues to hard tissues effectively avoids the problem of stress concentration due to direct connection of the soft and hard tissues, solves the problem of weak biological fixation of the ligament graft-bone combination part in the existing single-phase tissue engineering ligament, and solves the problem that the seed cells cannot enter the deep part of the ligament in the traditional ligament weaving method; the ligament graft has the complete weaving structure, and solves the problem of weak connection between layers in the existing three-phase tissue engineering ligament.

The invention provides a method for building monoclonal mesenchymal stem cells and an application of the method. The method for separating the mesenchymal stem cells comprises the following steps: A) digesting an animal sample by using collagenase, so as to disperse cells contained in the animal sample and to obtain a cell mixture containing stem cells; B) introducing nucleic acid molecules of encoding reporter protein into at least one part of cells of the cell mixture, wherein the nucleic acid molecules of encoding the reporter protein can be connected with specific promoters of the mesenchymal stem cells in a maneuverable manner, so as to express the reporter protein in the mesenchymal stem cells; and C) sorting by using an FACS (facial action coding system), and obtaining the mesenchymal stem cells of expressing the reporter protein, wherein the mesenchymal stem cells are in a single cell form. By adopting the method, the mesenchymal stem cells in the single cell form can be effectively prepared.

The invention provides a bovine theca cell in vitro culture modifier. A bovine theca cell in vitro culture solution is taken as a substrate, and melatonin is contained in the substrate. When the in vitro culture solution where the melatonin is added to culture bovine theca cells, the melatonin can inhibit synthesis of bovine theca cell progesterone, the melatonin inhibits StAR and CASP3 expression induced by LH+IGF1, but has no influence on CYP11A1 and CYP17A1 expression. However, the melatonin can promote bovine theca cell proliferation, the melatonin serves as an endocrine regulating factor of a bovine ovary function, effects of LH and IGF1 are inhibited by down-regulation of synthesis of steroid hormones, and membrane cell differentiation is delayed. The melatonin has good application values by being applied to preparation for promoting bovine theca cell proliferation or inhibiting steroidogenesis.