31results about How to "Easy to differentiate" patented technology

In an embodiment, an image is provided to an augmented reality application program. The program detects colors and modifies the image. In particular, the program may analyze an image of a scene provided by a camera of a portable electronic device that may be problematic for color challenged users. It then modifies one or more colors such that a color challenged user viewing the altered image may perceive the scene colors as the colors would be perceived by a non-color challenged user viewing the scene.

The invention belongs to the technical field of forest creatures, and discloses a cultivation method for quickly obtaining lycium ruthenicum regeneration seedlings. The cultivation method comprises the following steps: disinfection of seeds, obtaining of germfree seedlings, induction of a callus, obtaining of adventitious buds and induction of roots. According to the cultivation method, the callus is directly obtained from the germfree seedlings of lycium ruthenicum, and the lycium ruthenicum regeneration seedlings are cultivated through a tissue culture method; compared with a conventional method for inducing the callus through leaves or stems of the seedlings, the cultivation method has the advantages that the tissue culture time is greatly shortened, and cultivation cost of the lycium ruthenicum is greatly reduced; meanwhile, the cultivation efficiency is effectively improved, and the transplanting survival rate of the regeneration seedlings reaches up to 98 percent. The lycium ruthenicum obtained through the cultivation method has the characteristics of being high in genetic stability, low in cost, short in seedling growing period, high in reproduction coefficient, and easy and convenient to operate.

The invention discloses a method for artificial induction of differentiation of multipotential stem cells into neural precursor cells. The method comprises the following steps: performing in-vitro amplification culture on embryonic stem cells; performing subculture on the embryonic stem cells; and performing induction differentiation and in-vitro amplification on the embryonic stem cells into neural precursor cells. The invention discloses the method for artificial induction of differentiation of the multipotential stem cells into the neural precursor cells, in-vitro culture is implemented in a colony mode, peripheral cells can be relatively easily differentiated since cells in the middle of a colony are dense and the cells have relatively intense mutual action, meanwhile, donor cells used in the method are sufficient in source, high in purity and free of pollution, and thus a novel way is provided for acquiring nerve cells.

The invention discloses a novel culture method for in vitro differentiation from human embryonic stem cells to functional myocardial cells. The novel culture method is different from a conventional typical hanging-drop culture method. According to the novel culture method, an embryoid body is formed by adopting a simplified suspension differentiation culture method, serum-free culture liquid is adopted for improvement, and various inducing and nutrition-enriching substances are added into the culture liquid to increase the differentiation rate of the myocardial cells and prolong the survival time of the myocardial cells; a method for differentiation from embryonic stem cells to myocardial cells is further effectively updated and perfected, the proportion of the myocardial cells differentiated by the embryoid body is greatly increased, and a large quantity of myocardial cell sources with powerful functions can be provided for stem cell clinical transplantation treatment technology, drug screening and the like; the method is simple and feasible to carry out, the culture time is shortened, the functions for in vitro differentiation from the embryonic stem cells to the myocardial cells are improved and include pulsation time as well as autorhythmicity and rhythmicity of myocardial cell beating, and the method is good in repeatability.

The invention relates to prevention and treatment of bone diseases caused by glucocorticoid medicines. Concretely, the invention relates to application of Wnt signal pathway to prevention and treatment of bone diseases caused by glucocorticoid medicines. Expression of C / EBPalpha factor is down regulated at a later period that BMP2 induces a mouse mesenchymal stem cell line C3H10T1 / 2 to be differentiated into osteoblast, and overexpression of the factor can inhibit differentiation of osteoblast. The reason causing down-regulated expression of C / EBPalpha is that distal promoters (-1286bp / -1056bp) of C / EBPalpha are subjected to hypermethylation at the differentiation later-period of osteoblast and are silenced. Interruption of the methylation process promotes high expression of C / EBPalpha and leads to osteogenic / adipogenic differentiation unbalance. A Dex-induced osteoporosis model proves that Wnt / beta-catenin signal pathway has important regulation and control effects on expression and methylation of C / EBPalpha in differentiation of osteoblast. A stimulant / activator of the pathway is capable of rescuing osteogenic / adipogenic differentiation unbalance caused by Dex.

The invention discloses a propagation method of vernonia amygdalina. The propagation method is characterized in that a proliferation bud of the vernonia amygdalina is pre-cultured in a rooting culture medium to obtain an aseptic seedling, then the aseptic seedling is cut to be cultured in a matrix, and after the aseptic seedling is rooted and has new leaves, the aseptic seedling is then transplanted into a flowerpot. The vernonia amygdalina is propagated by combining a tissue culture technology and the cutting technology, so that complementary advantages can be realized, and the pre-cultured and non-rooted aseptic seedling is cut into a mixed matrix of peat soil, river sand and vermiculite to be rooted, and the difficulty that the culture medium is washed, so that the seedling is likely to infect harmful bacteria can be overcome, and the survival rate is increased; a great amount of cutting propagation materials can be provided all year round by adopting the tissue culture technology, and the mass production and all-year production requirements of seedlings can be met.

The invention relates to a tissue culture propagation method of Polygonatum multiflorum, comprising the steps of in an aseptic environment, collecting embryo from mature seeds of Polygonatum multiflorum by peeling, and sterilizing the embryo; inoculating the sterilized embryo to a callus induction medium, and performing light-free culture for 7-12 days so that the embryo expands to form a callus;cutting the callus into small pieces, inoculating the pieces to a callus subculture medium, and culturing for 18-25 days under light intensity of 800-1000 lux and daily lighting time of 10-12 h; cutting the callus subjected to subculture in step S3 into small pieces, inoculating the pieces to a sprouting medium, and culturing for 28-35 days under the light intensity of 1200-1800 lux and daily lighting time of 10-12 h, and inducing to obtain cluster buds; inoculating the robust single ones of the cluster buds to a rooting medium, and performing rooting culturing. The method herein helps greatlyreduce pollution rate of a tissue culture process, and improve propagation efficiency and seedling quality.

The invention discloses a combined bioartificial liver supporting system. The supporting system at least comprises branch pipelines connected in order, the branch pipelines are a blood input branch pipeline of which an upstream tail end is arranged to be a blood input end, a first slurry separating branch pipeline at least including a first plasma separator, a non- biological cleaning branch pipeline at least including a plasma hemoperfusion apparatus and a bilirubin absorber, a biological cleaning branch pipeline at least including a liver cell culture tank component, a plasma returning branch pipeline of which downstream tail end is arranged to be a blood output end; tee devices are connected among the first plasma separating branch pipeline, the biological cleaning branch pipeline and the non-biological cleaning branch pipeline, between the outlet end of the non-biological cleaning branch pipeline and the biological cleaning branch pipeline, and between an outlet end of the non-biological cleaning branch pipeline and the plasma returning branch pipeline; the tee devices comprise tee joints and branch paths connected with the tee joints; at least two branch paths are provided with current limiting pieces and connection pieces.

The invention belongs to the technical field of composite scaffolds and discloses a composite scaffold for improving gaps and pores to promote the cell adhesion rate and a preparation method. A channel pore and a spherical pore of the composite scaffold are 150mum-650mum and 3mum-15mum respectively; the preparation method comprises the following steps: obtaining a CS / HA (chitosan / hydroxyapatite) scaffold with pores and spherical pores by adopting an in situ hybridization technology and a freeze-drying method; connecting CS and RGD (arginine-glycin-aspartate) peptides with hydrogen bonds, and modifying the RGD (arginine-glycin-aspartate) peptides on the surface of a porous channel of the CS / HA scaffold with a static self-assembly method, so as to prepare a three-dimensional porous CS / HA scaffold with efficient adherent cells containing the RGD peptides. The scaffold has better cytocompatibility; through composition with RGD, the functions of attraction, induction and growth control of the three-dimensional porous CS / HA scaffold on cells are obviously enhanced; a physical absorption method and a chemical fixation method are combined in the preparation method, so that the material hasbetter adsorbability and stability.

The invention discloses an in-vitro inducing method for a high-expression Nurrl gene of a human mesenchymal stem cell, comprising the following step of inducing the high-expression Nurrl gene of the human mesenchymal stem cell in a DMEM (Dulbecco's Modified Eagle's Medium) / F-12 cultivation system in which RA (Retinoic Acid), CAMP (Cyclic Adenosine Monophosphate) and SHH (Sonic Hedgehog Homolog) are added. By using the in-vitro inducing method instead of a gene modification method, the expression of the Nurrl gene of the mesenchymal stem cell can be remarkably improved (about 23 times that before induction), the Nurrl gene of the mesenchymal stem cell is enabled to differentiate towards the dopaminergic neuron more easily, risks brought by gene modification is avoided, and a more ideal seed cell is provided for the clinical. Compared with an embryonic stem cell and a nerve stem cell, the mesenchymal stem cell is convenient to obtain materials, easy for in-vitro amplification, low in immunogenicity and capable of meeting the requirement of ethics.

A method of associating wireless devices with a wireless medical body area network, MBAN, where the wireless MBAN comprises at least one host is provided. The method comprises activating the host to search for wireless devices in range; displaying a list on the host of available wireless devices in range, wherein displaying the list comprises displaying each wireless device on the list with a unique representation on the list and the same unique representation on the wireless device itself; selecting a wireless device on the list; and associating the selected wireless device on the list with the host.

The invention discloses an SAR image super-resolution reconstruction method based on Euclidean distance regularization. First of all, multiple frames of an SAR image, with closer time phases are input and registered, and the multiple frames of the SAR image are placed in a grid and are processed to an SAR observation image with uniformly distributed pixels; then on the basis that an SAR image degradation model is established, a high-resolution SAR image reconstruction formula based on Euclidean distance double-side fully differential regularization is constructed; next, the observation image is initialized, and afterwards, according to the reconstruction formula, iteration estimation is performed on the high-resolution SAR image by use of a calculable optimization mode; and finally, outputting an estimated image as an SAR image super-resolution reconstruction result. According to the invention, a double-side fully differential mode of a Manhattan distance in SAR image reconstruction is changed to a Euclidean distance form, and prior knowledge is more reasonably described; and a substitution formula of the reconstruction formula is constructed for the purpose of solving the problem of differential solving in an optimization iteration process, and thus on an optimization solving calculable basis, the accuracy of the SAR image super-resolution reconstruction result is improved.

The invention relates to a method for increasing memory stem cell phenotypes by using culture medium cultured CD19 CART cells containing IL7 and IL-15 cell factors, and belongs to the field of biotechnology. Specifically, the used IL7 and IL-15 culture medium cultivated CD19CART cells are more easily to be differentiated into memory stem cell phenotypes, and IL7 and IL-15 preteatment used for T cells indicates that the memory T cell function and CAR-T cell anti-tumor activity can be improved.

The invention discloses a culture medium and a method for improving genetic transformation efficiency of tobacco Honghuadajinyuan. The culture medium comprises a co-culture medium and a selective culture medium; the co-culture medium and the selective culture medium each comprise an MS basic culture medium, NAA and 6-BA, the final concentration of the NAA is 0.41 mg / L, and the concentration ratioof the NAA to the 6-BA is 1:5; the method comprises the following steps: preparing a tobacco Honghua Dajinyuan leaf disc; infecting with agrobacterium tumefaciens; carrying out selective culture; andcarrying out rooting culture. According to the method, the budding rate (differentiated buds / leaf discs) of tobacco calluses reaches 127%, and the rooting rate (rooted seedlings / differentiated buds) reaches 66%; compared with an original method (the budding rate is 62%, and the rooting rate is 10%), the budding rate is increased by about 2 times, and the rooting rate is increased by about 6 times;and the requirement for large-scale genetic transformation is met.

The invention belongs to the technical field of plant tissue culture, and provides a method and a culture medium for culturing peony cotyledons to directly form somatic embryos. The method comprises the following steps: inoculating embryos of peony seeds into a peony aseptic seedling culture medium, sequentially carrying out dark culture and light culture to obtain peony aseptic seedlings, then inoculating cotyledons of the peony aseptic seedlings into a peony somatic embryo formation culture medium, and sequentially carrying out dark culture and light culture to obtain the peony somatic embryos. The method provided by the invention solves the problems of difficult survival of tissue culture materials and lack of effective test materials in subculture proliferation due to high pollution rate in the peony tissue culture process, and improves the application efficiency of the peony tissue culture technology.

A method for co-culturing with nerve stem cells to inducing rASCs to be DA Neurons by Lmx1a mediation, belongs to a method for treating Parkinson's disease by inducing stem cells to replacing defective Neuron cells. The method of the invention comprises steps of: building Lmx1a gene clone, recombining plasmid, building, packaging and recombining adenovirus Ad-Lmx1a, and co-culturing rASCs and NSCs which are infected by Ad-Lmx1a to differentiating them to be DA Neurons; and culturing rASCs and NSCs passage cells. Protein marker for expressing dopaminergic neuron cells can be differentiated from inducted neuron-like cells in the invention, and the inducted cells can transmit electric signal or secrete neurotransmitters, to prove that the inducted cells are the Neuron cells.

The invention discloses a planting method for mixed planting of Australian mangoes and custard apples. The planting method comprises the following steps of planting mango and custard apple seedlings in an interlaced manner, grafting Australian mango scions on branches of the mango seedlings, reserving main branches and auxiliary main branches according to round-head-shaped crowns, using second-layer auxiliary main branches as fruiting branches , and trimming fruiting trees four times every year; and after new buds of the custard apple seedlings germinate, reserving main branches and branches to be prepared into open-center tree shapes, and trimming fruiting trees three times every year. According to the planting method, by adjusting the custard apple production period, the Australian mangoes and the custard apples are planted in a mixed manner, the space, nutrient and water competitive relation of the two kinds of fruit trees in the annual growth period is reduced, and the utilizationefficiency of limited land resources is improved; and compared with the prior art, the economic benefits are improved by more than one time in the technical scheme.

The invention relates to a Chinese narcissus callus preservation and tissue culture method. The method comprises the steps of material selection, induction culture and callus preservation. When a Chinese narcissus callus preservation and culture medium provided by the invention is put into use, the reproduction coefficient of Chinese narcissus callus can be 5.16 times, and the callus grows well, has a high reproduction speed and is free from browning and differentiation phenomena. According to the Chinese narcissus callus preservation and tissue culture method, the problems of difficult subculture and preservation, low reproduction coefficient, serious browning and differentiation and the like of the Chinese narcissus callus can be solved, the practicability is high and the generalization performance is good. The callus preserved by the method is easy to differentiate when being transferred to a differential medium, and can serve as an excellent material of a receptor for genetic transformation of Chinese narcissus.

The invention discloses a shrimp ecological culture and a polyculture method based on facility microalgae culture, including preparing corresponding microalgae and shrimp fry; then putting them into a culture tank for culture; The cleaning control system monitors the breeding ponds; finally, the breeding is continued; the cleaning control system includes an algae recording unit, a data recording unit, a content detection unit, a single database, a data prediction unit, a single shrimp acquisition unit, a processor, a display unit, It is recommended to generate a unit and a management unit; the present invention uses the cleaning control system to monitor the number of microalgae in the breeding pond in real time, and also combines the growth of shrimp, the number of breeding days and the relevant feeding amount to reasonably predict the corresponding time. The number of microalgae will reach the corresponding upper limit, and according to the number that reaches the upper limit, it will be determined whether local targeted cleaning or overall cleaning is required; it is easy to treat differently.

The invention relates to the technical field of cell engineering tissue culture, and aims to provide a method for effectively preserving embryogenic callus of Acorus calamus. The method for effectively preserving the embryogenic callus of Acorus iris includes the steps of material collection, callus induction culture, screening and preservation of the embryogenic callus. The invention uses the young embryos of calamus calamus as explants, which has extremely low pollution rate, high callus induction rate, good embryogenicity of callus, and high reproduction coefficient, and at the same time solves the problem of easy browning and differentiation in the preservation of calamus calamus callus Serious problems, strong practicability, easy operation and good promotion.

The invention relates to a tissue culture propagation method of Polygonatum multiflorum, comprising the steps of in an aseptic environment, collecting embryo from mature seeds of Polygonatum multiflorum by peeling, and sterilizing the embryo; inoculating the sterilized embryo to a callus induction medium, and performing light-free culture for 7-12 days so that the embryo expands to form a callus;cutting the callus into small pieces, inoculating the pieces to a callus subculture medium, and culturing for 18-25 days under light intensity of 800-1000 lux and daily lighting time of 10-12 h; cutting the callus subjected to subculture in step S3 into small pieces, inoculating the pieces to a sprouting medium, and culturing for 28-35 days under the light intensity of 1200-1800 lux and daily lighting time of 10-12 h, and inducing to obtain cluster buds; inoculating the robust single ones of the cluster buds to a rooting medium, and performing rooting culturing. The method herein helps greatlyreduce pollution rate of a tissue culture process, and improve propagation efficiency and seedling quality.

The invention discloses a prawn ecological culture and polyculture method based on facility microalgae culture. The prawn ecological culture and polyculture method comprises the following steps of preparing corresponding microalgae and prawn seeds; then putting the microalgae and the prawn seeds into a culture pond for culture; carrying out feeding in the later culture process, and carrying out culture monitoring on the culture pond by means of a cleaning control system; and finally, continuously carrying out breeding. The cleaning control system comprises an algae recording unit, a data recording unit, a content detection unit, a single database, a data prediction unit, a single shrimp acquisition unit, a processor, a display unit, a suggestion generation unit and a management unit. By means of the cleaning control system, the number of the microalgae in the culture pond is monitored in real time, meanwhile, the growth condition of prawns, the number of culture days and the related feeding amount are combined to reasonably predict when the corresponding microalgae can reach the corresponding upper limit value, and according to the number of the microalgae reaching the upper limit, whether local targeted cleaning or integral cleaning is needed or not is judged; and differential treatment is facilitated.

The invention discloses a propagation method of Vernonia amygdala. Specifically, the secondary buds of Vernonia amygdala are placed in a rooting medium for pre-cultivation to obtain aseptic seedlings, and then the aseptic seedlings are cut into the substrate for cultivation. After rooting, Plant in pots after new leaves have grown. The present invention combines tissue culture technology and cutting technology to propagate Vernonia amygdala to achieve complementary advantages of the two. The pre-cultivated and unrooted aseptic seedlings are cut into the mixed matrix of peat soil, river sand and vermiculite to take root, which overcomes the need for cleaning and cultivation. It is helpful to improve the survival rate due to the problems of root and susceptibility to pathogenic bacteria; tissue culture technology can be used to provide large-scale cutting propagation materials all year round, meeting the requirements of large-scale production and annual production of seedlings.

The invention discloses an SAR image super-resolution reconstruction method based on Euclidean distance regularization. First of all, multiple frames of an SAR image, with closer time phases are input and registered, and the multiple frames of the SAR image are placed in a grid and are processed to an SAR observation image with uniformly distributed pixels; then on the basis that an SAR image degradation model is established, a high-resolution SAR image reconstruction formula based on Euclidean distance double-side fully differential regularization is constructed; next, the observation image is initialized, and afterwards, according to the reconstruction formula, iteration estimation is performed on the high-resolution SAR image by use of a calculable optimization mode; and finally, outputting an estimated image as an SAR image super-resolution reconstruction result. According to the invention, a double-side fully differential mode of a Manhattan distance in SAR image reconstruction is changed to a Euclidean distance form, and prior knowledge is more reasonably described; and a substitution formula of the reconstruction formula is constructed for the purpose of solving the problem of differential solving in an optimization iteration process, and thus on an optimization solving calculable basis, the accuracy of the SAR image super-resolution reconstruction result is improved.

The invention discloses an in-vitro inducing method for a high-expression Nurrl gene of a human mesenchymal stem cell, comprising the following step of inducing the high-expression Nurrl gene of the human mesenchymal stem cell in a DMEM (Dulbecco's Modified Eagle's Medium) / F-12 cultivation system in which RA (Retinoic Acid), CAMP (Cyclic Adenosine Monophosphate) and SHH (Sonic Hedgehog Homolog) are added. By using the in-vitro inducing method instead of a gene modification method, the expression of the Nurrl gene of the mesenchymal stem cell can be remarkably improved (about 23 times that before induction), the Nurrl gene of the mesenchymal stem cell is enabled to differentiate towards the dopaminergic neuron more easily, risks brought by gene modification is avoided, and a more ideal seed cell is provided for the clinical. Compared with an embryonic stem cell and a nerve stem cell, the mesenchymal stem cell is convenient to obtain materials, easy for in-vitro amplification, low in immunogenicity and capable of meeting the requirement of ethics.

The invention belongs to the technical field of forest creatures, and discloses a cultivation method for quickly obtaining lycium ruthenicum regeneration seedlings. The cultivation method comprises the following steps: disinfection of seeds, obtaining of germfree seedlings, induction of a callus, obtaining of adventitious buds and induction of roots. According to the cultivation method, the callus is directly obtained from the germfree seedlings of lycium ruthenicum, and the lycium ruthenicum regeneration seedlings are cultivated through a tissue culture method; compared with a conventional method for inducing the callus through leaves or stems of the seedlings, the cultivation method has the advantages that the tissue culture time is greatly shortened, and cultivation cost of the lycium ruthenicum is greatly reduced; meanwhile, the cultivation efficiency is effectively improved, and the transplanting survival rate of the regeneration seedlings reaches up to 98 percent. The lycium ruthenicum obtained through the cultivation method has the characteristics of being high in genetic stability, low in cost, short in seedling growing period, high in reproduction coefficient, and easy and convenient to operate.

The invention provides a rainwater collecting and recycling system. The system comprises a roof rainwater collecting device used for collecting roof rainwater and storing the roof rainwater in a roof water storage tank, a road surface rainwater collecting device used for collecting rainwater on the road surface and storing the rainwater in an underground water storage tank, a monitoring device used for monitoring the water level of the roof water storage tank, and a control device used for controlling a drain valve corresponding to the roof water storage tank to be opened under the condition that the monitoring device monitors that the water level in the roof water storage tank meets a preset condition. Under the condition that the monitoring device detects that the water level in the roof water storage tank does not meet the preset condition, the drain valve corresponding to the underground water storage tank is controlled to be opened, so that the rainwater is conveyed to the rainwater recycling device to be recycled.