The present invention relates to a rapid lotus corniculatus l. breeding method, which comprises the following steps: 1) sterilizing lotus corniculatus l. seeds; 2) placing the sterilized and washed lotus corniculatus l.seeds in a culture dish under a sterile state, and culturing for 3-5 days in an illumination and moisture environment to germinate; 3) transferring the germinating seeds obtained from the step 2) in a hormone-free MS base culture medium, wherein a room temperature is 25-27 DEG C, light intensity is 2000 lux, an illumination time every day is 12 h, and a culture time is 20-25 days; 4) taking the bud after a length of the bud is 10-12 cm, and cutting under a sterile condition, wherein a length of every stem segment is about 1.5-2 cm, and has a mature leaf; 5) transferring the stem segment into a disposable growth culture medium; and 6) after cutting the stem segment, placing in a certain environment, wherein a room temperature is 25-27 DEG C, light intensity is 2000-3000 lux, an illumination time every day is 12 h, and a culture time is 20-25 days. With the present invention, a culture period is short, operation management and application promotion are easily achieved, production cost can be significantly reduced, transplantation survival rate is significantly increased, and vitreous shoot phenomenon can be completely eliminated.