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Rapid rhizoma polygonati propagation technology method

A fast and advanced technology, applied in the field of bioengineering, can solve the problems of insufficient seed source, high production cost, long production cycle, etc., and achieve the effect of large reproduction coefficient, low production cost and low pollution rate

Active Publication Date: 2014-06-18
湖南宏邦生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large amount of seeds used, the high production cost, and the lack of provenance, rhizome propagation cannot meet the production needs; although the seed reproduction coefficient is large, there are two physiological dormancy periods, and the production cycle is long. Under natural conditions, it takes three years to grow a true leaf

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The mature fruit picked back, rubbed off the exocarp, layered with fine sand and placed in a refrigerator at 3°C ​​for 30 days, and then treated with 200ppmGA 3 Soak for 24 hours, put it into an incubator at 26°C for constant temperature cultivation, the germination rate reaches 70%, and then soak it in 200ppm KT for 12 hours, the germination rate can reach 90%. Most can be sown to outdoor seedlings.

[0017] Remove the fibrous roots of the germinated small underground rhizomes, wash them in running water for one day and one night, treat them with 8% calcium hypochlorite for 30 minutes, disinfect them with 70% ethanol three times in an ultra-quiet bench, and disinfect them with 0.1% mercury chloride for 8 minutes. Rinse with water for 5 times, cut small rhizome buds longitudinally, inoculate into solid medium of MS plus 6-BA1.0mg / L\2,4-D0.5mg / L\sucrose 30g / L\agar 7g / L, PH5.8 Cultivate under the conditions of temperature 26℃, light time 16h\light intensity 1500Lx, and c...

Embodiment 2

[0019] The refrigerated seeds were treated with 400ppmGA 3 Soak for 12 hours, put it in an incubator at 26°C for constant temperature cultivation, and the germination rate is about 40%. After the radicle grows, soak it in 300ppm KT for 12 hours, and the germination rate is about 80%.

[0020] After the callus was induced completely according to the S1 method, the difference was that it was first transferred to MS plus TDZ1.5mg / L\2,4-D1.0mg / L\sucrose 30g / L\agar 7g / L medium for 15 Transfer to MS and add 6-BA1.0mg / L\NAA2.0mg / L\sucrose 30g / L\agar 7g / L on the differentiation medium culture induction again, the cluster bud of every piece of callus reaches 8.9; S2.

[0021] Compared with S1 and S2, the germination rate of seed treatment S1 increased by 30% compared with S2, and the bud germination rate increased by 10%. In the process of tissue culture, according to the results of differentiation culture, S2 had 2.4 more shoots per callus on average than S1.

[0022] The above spe...

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Abstract

The invention provides a rapid rhizoma polygonati propagation technology method. The method comprises the following steps: breaking seed dormancy by changing temperature treatment and hormone treatment, performing induction in vitro to culture callus by utilizing seeds subjected to dormancy breakage as materials, performing differentiation culture and root induction, and performing vegetative propagation on rhizoma polygonati seedlings. By adoption of the technology, the rhizoma polygonati propagation factor can be greatly improved, the production period is shortened, the sowing quantity is reduced, and the production cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and in particular relates to a technical method for rapid propagation of the traditional Chinese medicine Rhizoma Polygonatum. Background technique [0002] Polygonatum is a perennial herb of the Liliaceae Polygonatum. There are about 41 species in the world, and about 35 species in my country. According to the "Chinese Pharmacopoeia 2010 Edition", the medicinal products in my country are mainly Rhizoma Polygonatum distributed in the north, Polygonatum Polygonatum distributed in the Yunnan-Guizhou Plateau and Polygonatum multiflora in the south of the Yangtze River. It has a history of more than 2,000 years for medicinal purposes. Because of its wide distribution, strong adaptability, high medicinal value, and both medicine and food, it has attracted more and more attention, especially as a functional food, it has a very broad application prospect. [0003] Polygonatum mainly contains po...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 张旺凡
Owner 湖南宏邦生物科技有限公司
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