162 results about "Budding" patented technology

Construction systems for erecting budding structures comprise a plurality of prefabricated interconnectable modular budding units, each unit comprising framing members and a plurality of nodes, each node situated for selective interconnection with other units, the nodes and the exterior dimensions of the frame conforming to ISO shipping standards such that each unit is transportable using the ISO intermodal transportation system, and such that when the units are interconnected, a building structure is formed. The modular units are assembled at a remote location, and are there constructed to a semi-finished state, following which the semi-finished modular units are transported from the remote location to the job site, where they are secured to form the structure being erected, and the semi-finished modular units are thereafter constructed to a finished state.

The invention relates to the agricultural field, which is a method for improving output and quality of lucern seeds, and comprises selecting and preprocessing land blocks, selecting grass seeds and breaking dormancy of lucern seeds, producing nodule bacteria into bacteria liquid, spraying the bacteria liquid to seeds in the dark, low-temperature and humid environment, and fully and evenly churning the nodule bacteria with the seeds, and planting and sowing, the occupied area of each seedling is 0.24-0.35m 2, adopting mild irrigating and fertilizing weedicide to weed, penetratingly irrigating for a time in the early stage of budding and flowering, and further watering in flowering and pod-bearing period, auxiliary pollinating by human labor and preventing and curing pests, when 2 / 3-3 / 4 of green pods of lucerns become brown and seeds become yellow, the seeds of lucerns are mature, and people can reap, and thinning out of branches and planting after seeds are reaped. The invention overcomes the shortcomings in the prior art and controls line space and planting distance to continuously achieve the highest seed output in many years.

The invention discloses a method for minitype quick breeding of sympodial bamboos nursery, which uses fasciculate bamboo shoots as minitype stock plant to be planted in a nursery for breeding new bamboos; when the root of stalk of the new budding bamboo grows, the new bamboo and the stock plant are separated and cultured in situ; when the root of the new bamboo grows and the lateral branch draws out, the new bamboo is implanted on seeding bags for culturing into seedlings of complete plantlet. The method provided by the invention is wide in application, simple in operation, low in cost and high in efficiency, and is a new technology with wide market prospect for bamboo plant breeding.

The invention discloses a mulberry panicle-sprout grafting-cuttage seedling raising method which enables mulberry to become good mulberry varieties in one step. The method comprises the following steps: selecting and preparing materials; grafting in a single-branch multi-sprout way; bagging nutritional soil; cutting and planting a grating body; managing after the grafting; transplanting a seedling; and managing after transplantation. The invention uses Japanese mulberry, hybrid mulberry, Chinese mulberry 5801, Fengchi mulberry, Guangxi excellent mulberry series, Guangdong mulberry series, Hunan hybrid mulberry series, Nongsang series, mulberry series and the like which can easily take roots and survive after cuttage, as the tree stock; a mulberry winter budding technique is adopted for multi-sprout grafting the excellent panicle sprouts on the branch of the tree stock; and a single sprout is planted into the nutritional soil to germinate and grow, thereby directly cultivating an excellent mulberry seedling. The seedling raising method has the advantages of cost saving, high efficiency and high speed. The invention can be used not only for raising and breeding mulberry seedlings, but also for one-step orchard establishment and fix planting. The invention promotes the excellent mulberry to put into production and has important value.

The invention belongs to the technical field of plant budding, particularly relates to a method for stopping Taxus chinensis var. mairei seed dormancy, and solves the technical problem of slow budding, low budding rate and the like. The method includes the steps of A, soaking Taxus chinensis var. mairei seeds in sulfuric acid 55-65% in mass concentration for 2-4 hours, and washing the soaked seeds with flow water for 12-36 hours; B, soaking the acid etched seeds in pre-prepared solution for at least 3 hours, and soaking the soaked seeds in clean water for at least 3 hours; and C, performing low-temperature stratification or variable-temperature stratification to the seeds soaked by clean water. The method has the advantages that the method is time-saving, effective and economical, the problems that dormancy is deep, budding is difficult and budding rate is low are solved, standards are provided for seed seedling market circulation, seed quality is guaranteed, and great significance is brought to breeding cultivation development of Taxus chinensis var. mairei.

In this application are described filovirus-like particles for both Ebola and Marburg and their use as a diagnostic and therapeutic agent as well as a filovirus vaccine. Also described is the association of Ebola and Marburg with lipid rafts during assembly and budding, and the requirement of functional rafts for entry of filoviruses into cells.

A dormancy waking up and germinating method for the seeds of notopterygium includes properly changing the temp to wake up said seeds in dormanant state, promoting the germination of said seeds, and controlling the temp, culture medium and sowing depth to obtain high-quality seedlings.

The invention discloses a method for inhibiting a bud through a bud in organic flue-cured tobacco production, which comprises the steps of: topping during flower budding and center flower blooming of tobacco plants in a tobacco field, wherein 18-22 leaves are left for each tobacco plant during topping, and 1-2 leaflets are left below an inflorescence at the same time; and suckering when the axillary bud successively germinates to 5-10cm after topping, wherein 3-5cm of branch is reserved at the root of the tobacco branch. Compared with the traditional manual bud picking method, the method disclosed in the invention achieves the purpose of inhibiting the growth of the axillary bud, the bud picking can be reduced by 2-3 times per tobacco plant during the growth period in the field, the cost for labor is saved by 60-90 yuan per mu, the labor cost is averagely saved by 33-43%, the working efficiency of the organic flue-cured tobacco production is improved, and an effective method is provided for reducing the chemicals investment in the pollution-free, green flue-cured tobacco production or conventional flue-cured tobacco production.

The invention provides a soaking and pregermination device for seeds. The device comprises a guide rail, a water supply device, sprayers, suspension rods, a heating coil pipe, ultraviolet sterilization lamps, a mobile cart device, pregermination trays and a drainage pipe, wherein left ends of the suspension rods are fixed on a tank through fixing plates; water distribution pipes are hung at bottoms of the suspension rods through lifting rings; a water supply pump is arranged on a warm water pipe; the ultraviolet sterilization lamps are hung at bottoms of the suspension rods; the pregerminationtrays are arranged at tops of layer racks; the pregermination trays are mutually connected through connection pipes; a draining cavity is formed in the bottom of each partition plate; water feeding and discharging ports are formed in the left end and the right end of each draining cavity; a draining electromagnetic valve is arranged on a draining pipe. According to the device, automatic control of soaking and pregermination of the seeds is realized, and the phenomena of germ death caused by too long seed soaking time and uneven budding caused by too high and too low temperature during buddingor the phenomenon of bud overheating caused by the fact that the seeds are not watered and cooled timely at high temperature are avoided.

The invention relates to a T-shaped bud grafting method for walnuts, which belongs to the grafting technology of the asexual propagation of the walnuts. The method comprises the steps of selecting and cutting a bud grafting part, cutting a scion bud, inserting the scion bud, treating a grafting opening, and binding the bud grafting part. Compared with the prior art, during the process of the grafting opening treatment, a flow guiding opening (5) is cut below the grafting opening, so as to enable bleeding sap to flow out of the flow guiding opening (5), therefore the problem that the bleeding sap erodes the scion bud slice in the other grafting methods is overcome, and the survival rate is remarkably improved and is more stable.

The invention discloses an appreciating landscape pavilion type tree crown modeling method. The method aims to solve the problems that a part of landscape trees are singular in crown type, lack of attractiveness and poor in ornamental value. According to the grafting modeling method, inverse budding is carried out on a center tree, budding is carried out on stand column trees, inverse budding and budding branches are subjected to inarching, and a pavilion type tree crown is formed. A unique model is formed, and artistic and ornamental value of landscape woods can be obviously improved. Natural corners are formed through the inverse budding method, perfect inarching of the corners and grafted sprouts on stand columns is achieved, and the corners and the grafted sprouts grow into a whole. According to the inarching method, contact faces formed by notches are large, formed union faces are large, and trees can easily survive. In addition, the branches after inarching are not completely cut off from parent bodies, the original shapes of parent body roots are maintained, only a part of grafted stems are cut off, then the cut parts of the branches and the cut parts of the grafted stems are tightly combined, and due to the fact that root systems of two trees absorb nutrition, the inarching survival rate is high.

The invention discloses a hormone-free rapid propagation organic production method of anoectochilus formosanus germchits. The method comprises the following steps of: step 1, sterilely germinating seeds, including disinfecting anoectochilus formosanus capsules and taking out the seeds, and inoculating the taken seeds on a culture medium I to be cultured until the seeds are germinated to protocorms; step 2, carrying out proliferation differentiation on the protocorms, including transferring the protocormss formed from seed germination to a culture medium II to have proliferation differentiation cultivation; and step 3, growing roots from strong seedlings, including transferring the protocorms to a culture medium III to have strong seedling root growth cultivation when the differentiation buddings of the protocorms grow to 1.5-2.5cm, and carrying out seeding hardening and transplantation when the seedlings grow to 5-6cm high and the roots are 2-3cm. According to the method, a technical route of seed, protocorm and germchit is adopted, and no any hormone is added into the culture mediums used during the cultivation process, so that on the basis that the rapid germination of the anoectochilus formosanus seeds is ensured, the purpose that the produced germchits have no residual exceeding is ensured, and germchits of high quality are provided for producing organic products of anoectochilus formosanus.

The invention relates to an efficient germination accelerating method for tomato seeds. The method includes the steps of: putting tomato seeds in clear water and selecting full tomato seeds; using a mixed disinfectant liquid to perform disinfection treatment; washing the disinfected tomato seeds with clear water and performing airing; soaking the aired seeds in a seed soaking agent for 2-6h; subjecting the soaked tomato seeds to ultraviolet treatment; placing the tomato seeds in a culture dish to undergo germination accelerating treatment; stirring the tomato seeds subjected to germination accelerating treatment with modified soil evenly according to a mass ratio of 1:4, and putting the mixture in a constant temperature culture chamber for culture till germination. According to the invention, the tomato seeds have fast germination speed, high budding rate and evenly growing bud seedlings, after transplantation into field, the seedlings have few diseases and pests and a developed root system, the transplantation survival rate is high, and the tomato yield is increased. At the same time, tomatoes planted by the germination accelerating method have high vitamin content, the planting cost is reduced, and the economic benefits are improved.

The invention discloses a method for making sour natto by fermenting budded soybean through fibrinolytic bacteria and probiotics. The method comprises the following steps of: 1, soybean budding: soaking soybean for 8 to 16 hours, and budding for 6 to 14 hours in dark place at the humidity of 50 to 80 percent and at the temperature of between 20 and 45 DEG C; and 2, microbial fermentation: flushing the budded soybean, adding salt in an amount which is 1 to 2 percent of the weight of the soybean and sugar in an amount which is 6 to 12 percent of the weight of the soybean, steaming or sterilizing for 15 to 90 minutes at the temperature between 100 and 121 DEG C, cooling to the temperature between 37 and 45 DEG C, inoculating 106-109cfu / mL microbial fibrinolytic bacteria and probiotics, controlling the total inoculation quality to be 2 to 14 percent of the total weight of the materials, performing closed fermentation for 1 to 3 days at the temperature between 30 and 43 DEG C, taking out, standing at the temperature of between 0 and 4 DEG C, and performing after-ripening for 12 hours to 7 days. The sour natto produced by the method is golden in appearance, moderate in hardness, sour, sweet, fragrant, tender and delicious; special ammonia taste in the conventional natto is eliminated; and the appearance color of the conventional natto is changed.

The invention relates to a method for rapid asexual propagation of an aurelia aurita, which comprises the following steps of: enabling a certain density of aurelia aurita scyphistomae attached to a polyethylene corrugated plate to be propagated rapidly through gemmatio under an appropriate condition, and enabling the scyphistomae to reach a certain density within a short time; transplanting the scyphistomae on the original corrugated plate to other clean corrugated plates according to the density of 0.5-0.7 per square centimeter; adding scyphistomae nutrient solution; enabling the scyphistomae to be propagated rapidly on the new corrugated plate under an appropriate condition after the scyphistomae is attached to the new corrugated plate; and carrying out strobilation on the scyphistomae through temperature control after the scyphistomae reaches a certain number so as to generate large number of aurelia aurita ephyrae and medusas. Due to the adoption of the method, a large numberof aurelia aurita scyphistomae, ephyrae and medusas which have high activity can be obtained without aurelia aurita adults. The method is rapid, simple and economical, and is suitable for obtaining an aurelia aurita material under a simple experiment condition.

The invention provides a quick efficient nostoc sphaeroides breeding method which comprises the following steps: (1) soaking nostoc sphaeroides spheres in purified water, carrying out light source induction, and collecting filter liquor when the nostoc sphaeroides spheres are spontaneously broken; (2) mixing the filter liquor with a hormogon culture solution, and performing aerobic culture under the conditions of constant temperature and constant light in a room to obtain microsphere-shaped nostoc sphaeroides; and (3) feeding the microsphere-shaped nostoc sphaeroides as sphere seed sources into next stage for expanded culture, continuously budding from the sphere seed sources by sub-spheres in a culture process and finally dropping to directly form independent microsphere-shaped nostoc sphaeroides. According to the method, the nostoc sphaeroides spheres are broken by adopting the induction process so as to obtain hormogon, the hormogon is cultured to obtain microspheres and finally the budding for breeding is carried out by virtue of the microspheres; for the whole breeding method, the breeding cycle is greatly shortened, the breeding process is simplified, no requirements on the purity of algae seeds are existent, the seed production quantity is large, the economy and practicality are achieved, and large-scale production of the nostoc sphaeroides is facilitated.

The invention relates to a microbial soil conditioner prepared from edible fungus bran and a preparation method thereof. The method comprises the following steps: using edible fungus bran with normal mushroom budding, and preparing leaching liquid and a conditioner carrier; evenly mixing bacillus megaterium liquid, bacillus mucilaginosus liquid and streptomyces hygrospinosus liquid which are fermented and cultured by the leaching liquid serving as a culture medium with the conditioner carrier prepared from the fungus bran respectively to prepare conditioner compositions; and finally evenly mixing three compositions in a certain proportion to obtain the microbial soil conditioner. The microbial soil conditioner prepared by the method is rich in various nutritions and active substances, canpromote soil to form a solid particle structure, effectively decompose organic P and K elements of the soil into an inorganic state for crops to absorb and utilize, and improve disease resistance of the crops to soil-borne disease. The preparation method for producing the microbial soil conditioner has skillful design scheme, realizes general and comprehensive utilization of the waste fungus branat high efficiency, and has low production cost and high added value of products.

The invention relates to a grafting method of sweet osmanthus. The grafting method of the sweet osmanthus includes following steps: (1) selecting rootstocks: selecting glossy privet and fraxinus chinensis in the south, and selecting Chionanthus retusus in the north; (2) selecting scions: selecting excellent single plants which grow strongly, do not suffer from plant diseases or insect pests, are pure in breed and flower exuberantly from a scion picking seed tree; (3) gathering the scions: gathering scions used for spring grafting before spring budding, and using the other scions for autumn plate budding and stem grafting once the autumn scions are picked; (4) processing the scions: cutting each scion for the stem grafting into a shoot section with 4-6 buds in the grafting, sealing the shoot sections with paraffin, and using a water bath method to melt the paraffin; (5) using a notch grafting method. Compared with other propagation methods, the grafting method of the sweet osmanthus has the advantages of being simple to operate and large in propagation amount, obtaining grafting trees rapid in growth and the like.

The invention relates to an oily sunflower planting method and application thereof in an early rice-oily sunflower-rape triple-cropping system. The oily sunflower planting method comprises the following steps: (1) selecting an improved variety, carrying out plowing, carrying out basal dressing, carrying out furrowing and compartment dividing, and carrying out pitting; (2) carrying out seed manure applying, carrying out sowing, and carrying out irrigation; and (3) carrying out field management and harvesting: carrying out resowing or replanting, and carrying out water drenching; carrying out final thinning, carrying out intertillage weeding, and covering compartment faces of oily sunflowers with rice straws; carrying out topdressing prior to budding of the oily sunflowers; carrying out moisture management after the budding of the oily sunflowers; carrying out disease and insect pest control; carrying out pollination or fertilization; and carrying out harvesting. The oily sunflower planting method disclosed by the invention can be applied to the early rice-oily sunflower-rape triple-cropping system. The oily sunflower planting method disclosed by the invention is high in oily sunflower yield, light in crop disease, insect and weed damage, low in chemical fertilizer consumption and low in cost, can be used for fertilizing soil fertility, improving soil and protecting cultivated land productivity, is water-saving and can be applied to early rice-oily sunflower-rape triple-cropping system planting. According to the application disclosed by the invention, through reasonably matching early rice-oily sunflower-rape varieties, the link-up of oily sunflower-rape crops for rotation is improved, and grain-oil double good harvest, i.e., 500kg of grains per mu and 200kg of oil per mu is achieved.

The invention discloses a growth regulator for promoting the advanced budding of daylilies, relating to the fields of growth regulation and control of plants. The growth regulator mainly contains melatonin, gibberellin, polyaspartic acid, sodium nitrophenolate, potassium dihydrogen phosphate, urea, zinc sulfate, Tween 20, ethanol and clean water. The growth regulator is integrated with the functions of plant growth regulating substances and mineral elements and can be used for realizing regulation and control from two aspects of plant hormone physiology and mineral nutrition, so that the budding time of the daylilies can be advanced, and the yield can be increased. The growth regulator is good in quality stability and has remarkable application effects; and by spraying the growth regulator, the budding time of the daylilies can be advanced by 18-24 days, the yield can be increased by above 5.0%, and partial quality is remarkably improved.

The invention relates to walnut grafting, in particular to a walnut bark grafting method without moisture preservation and shading. At present, the walnut grafting refers to grafting the buds or twigs of a quality variety to another exuberant walnut tree with stronger adaptability to local natural conditions, the adopted grafting methods are relatively complicated and have the defects of lower grafting survival rate, high cost and labor waste, and tree stocks are injured seriously with slow wound healing speed and need moisture preservation and shading treatment. The method comprise the steps of: twig collecting, scion treatment, tree stock treatment, bark grafting, binding treatment, bleeding sap treatment, budding and belt releasing, and the like, wherein a high-quality thinly-skinned walnut variety is used as a scion, and the measures of low-temperature storage, plastic mulching on grafting parts and the like are adopted. The invention has the advantages that the tree stock is injured lightly with easy healing grafting parts, the moisture preservation and shading are not needed, the survival rate is kept above 90 percent, the purpose of large-scale popularization is achieved, the problem on reproducing a walnut variety seedling is successfully solved, and the method has the value in large-scale popularization and application.

The invention discloses a double-cropping-rice-area oilseed-rape no-tillage and direct seeding cultivation method. The double-cropping-rice-area oilseed-rape no-tillage and direct seeding cultivation method includes the following steps that water draining and field drying are carried out in time before preceding-crop late rice is harvested, ditching and land preparation are carried out after the preceding-crop late rice is harvested, the ridge width is 133 cm to 150 cm, the furrow width is 30 cm, and the furrow depth is 15 cm; base fertilizer is fully applied, seeding is carried out in a no-tillage and drilling mode, and the amount of seeds which are seeded per mu is 350 g to 420 g; fertilizing is carried out during seedling stage and oilseed-rape budding and bolting, leaf surface spraying is carried out with borax liquid of 0.2% or monopotassium phosphate in the seedling stage and full-bloom and pod bearing stage respectively, and the setting percentage is increased; intertilling and weeding are carried out at fixed period; diseases and pests are controlled; at the time 30 days to 35 days before the oilseed-rape full-bloom stage end, seeds in pods become yellow from green, and when the color is red, harvesting can be carried out. According to the double-cropping-rice-area oilseed-rape no-tillage and direct seeding cultivation method, labor and time are saved, cost is saved, the contradiction of crops for rotation in the double-cropping-rice area can be released, the cropping system is improved, the land utilization rate is increased, and economic benefits are improved.

The invention discloses a pepper seed seedling raising method, and relates to the technical field of agricultural planting. The method comprises the following steps: (1) seed selection; (2) seed soaking; (3) seedbed making; (4) sowing; (5) seedbed management; (6) seedling transplanting. The seedling raising method has the advantages that temperature and illumination time of pepper budding are strictly controlled during seedling raising of pepper seeds, the seedlings are provided with nutrients in time, and grown pepper seedlings are thick, and have strong vitality and high survival rate; a series of standard procedures are included from seed selection to seedling stage management, and the method is simple and easy to operate and suitable for large-scale promotion.

The invention provides a method for promoting germination of seeds of Gentiana rhodantha and seedlings of the Gentiana rhodantha and relates to the technical field of plant seed treatment. The provided method for promoting germination of the seeds of Gentiana rhodantha comprises steps as follows: preparation of a seed treating fluid, seed soaking before seed germination, germination and budding and the like. As for the conditions that research about seed germination of Gentiana rhodantha is rare and large-area artificial planting of the Gentiana rhodantha does not exist, the innovative germination and seedling emergence method is provided, a foundation is provided for artificial planting of the Gentiana rhodantha, and sustainable utilization of Gentiana rhodantha resources is further promoted.

The invention relates to the technical field of grafting, in particular to a production method and a using method of a simple carya illinoensis budding knife. The methods can be applied to carya illinoensis as well as to fruit tree grafting and flower and tree grafting. The methods are characterized in that two congruent figure blades are fixed on two sides of a supporter via a firm support to enable length and shape of slices on a stock and a scion to be identical. By using of the budding knife, cutting frequency dung budding can be reduced, working efficiency is improved, two parts cut on the scion and the sock can be ensured to be closely fitted, and grating survival rate can reach above 95% and is increased greatly.

The invention discloses a manufacturing method for an artificial atractylis lancea seed. The method mainly comprises an optimum selection scheme of related action conditions like acquisition of an explant material, selection of an episperm coating material and compatibility of an endosperm adsorbent, a carbon source, culture medium concentration and hormone for manufacturing an artificial seed. The artificial seed manufactured by using the method in the invention can get rid of restrictions of season, temperature and humidity environments on the processes like generation, formation, budding, germination and the like of a natural atractylis lancea seed, and can produce a genuine traditional Chinese medicinal raw material of atractylis lancea through large-scale industrial propagation and breeding under the technical conditions of tissue culture and asexual propagation.

In this application are described filovirus-like particles for both Ebola and Marburg and their use as a diagnostic and therapeutic agent as well as a filovirus vaccine. Also described is the association of Ebola and Marburg with lipid rafts during assembly and budding, and the requirement of functional rafts for entry of filoviruses into cells.

The invention discloses a method for replacing the variety of a tea-oil tree by high-grafting in autumn, namely, a notch side graft. The method comprises the following steps: stock whittling: selecting a flat and straight part of a stock, and gashing an H-shaped scuffing on the selected part until the scuffing reaches a cambium; grafting: uncovering upper and lower cortexes of the stock along the middle part of the scuffing, embedding a scion, closing the cortexes of the stock, and enabling laminas and axillary buds to expose; and binding and cover adding: binding the closed part, then carrying out film cover adding and final-period management. By using the method disclosed by the invention, the defects that the survival rate of scion buds is affected by high temperature in summer and new treetops after crown grafting in early summer are easily damaged by freeze are overcome, the final-period management is simple, the purpose of improving yields rapidly after a variety change is performed can be achieved, the survival rate of scion buds is high (more than 90%), the treetop budding is neat, and the blooming rate of shoots formed at the second year of scion bud germination can reach 50%, therefore, the effect of rapid forming can be achieved, the cost can be saved, the fruiting can be advanced and the economic benefits can be improved; and meanwhile, the method can be easily accepted by producers and is convenient for promotion and application.

The invention relates to an in vitro clonal propagation technique for edible snake gourd root buds, which is characterized in that, edible snake gourd has strong characteristic of root budding; in early spring, a shed is arranged; carefully selected mother roots with good quality, high yield and resistance are sterilized and then separately planted; temperature is maintained between 10 DEG C to 40 DEG D; relative humidity is above 85%; the higher the temperature, the faster the germination; a mulch film is covered on trench surface, and a plurality of plant spacing holes are arranged; when the length of the root bud seedlings reaches 7cm to 10cm, the root bud seedlings are planted in the plant spacing holes; temperature ranges from 20 DEG C to 30 DEG C, and relative humidity is above 90%; after 25 days to 35 days, rooting takes place. The in vitro clonal propagation technique for edible snake gourd root buds has the advantages that, the bud seedlings of the invention is formed by the vegetative mass of female mother roots; good quality, high yield, resistance and other fine traits of the product can be maintained without variation; the invention has the characteristics of simple operation, high reproductive rate, low cost, good antibacterial property and other characteristics.

The invention discloses a method for picking buds by needle pricking to graft seedling buds for culturing seedling. When the seedling survives and ages after bud grafting, a lowest pair of leaves is retained after topping off before an axillary bud sprouts and a top stem is cut off; and then a pricking needle carries out the bud picking by needle pricking for a parent stock bud. The method has a simple process, simplicity and quickness. The needle pricking mode is used to solve the problems of foliage leaf drop-out, weak scion budding or no budding, low stocking percent, low bud picking speed and the like caused by improper bud picking. In addition, as the needle pricking area is small, the method basically has no damage to a leafstalk, effectively improves the bud picking speed, reduces the number of dropped leaves, brings about the robust scion, and improves the stocking percent.