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Quick efficient nostoc sphaeroides breeding method

An efficient and fast technology, applied in the field of cyanobacteria cultivation, to achieve the effects of simplifying the breeding process, shortening the breeding cycle, and increasing the growth and development speed

Active Publication Date: 2014-09-10
CHANGDE YANDI BIOTECH LTD CO +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is no report on the breeding method of adopting Ge Xianmi sphere budding propagation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The induction of embodiment 1 Gexian rice algae multiplication segment

[0028] Wash the Gexianmi spheres (diameter ≥ 3mm) and place them in an open container, soak them in purified water (it is appropriate to just immerse them), and place the container in an environment of about 500-10000lux for cultivation. 15-33°C. After the spheres of Gexianmi are completely broken, use a sieve to filter out the residue in the liquid, and collect the filtrate, which is dispersed with a large number of Gexianmi algae. The induction rate of this algae breeding section induction process is 100%.

Embodiment 2

[0029] The cultivation method of embodiment 2 Ge Xianmi algae section

[0030] ⅰ) Preparation of culture medium for algae breeding stage

[0031] Using purified water as the water source, the concentrations of various nutrients added are as follows:

[0032] MgSO 4 ·7H 2 O: 75mg / L; K 2 HPO 4 ·3H 2 O: 40mg / L; EDTA·2Na: 1mg / L; CaCl 2 2H 2 O: 9mg / L; Citric acid: 6mg / L; Ferric ammonium citrate: 6mg / L; Na 2 CO 3 : 20mg / L; H 3 BO 3 : 2.86g / L; MnCl 2 4H 2 O: 1.81g / L; ZnSO4 ·7H 2 O: 222 mg / L; Na 2 MoO 4 2H 2 O: 39mg / L; CuSO 4 ·5H 2 O: 79mg / L; Co(NO 3 ) 2 ·6H 2 O, 49mg / L.

[0033] There is no need to carry out any disinfection treatment on the culture medium of the algae breeding section, such as high-pressure steam sterilization.

[0034] ii) Cultivation of algae growth stage

[0035] The algae liquid collected in Example 1, which is dispersed with a large amount of Gexianmi algae growth section, is mixed with the algae growth section culture solution prepared ...

Embodiment 3

[0043] The budding propagation of embodiment 3 Ge Xianmi spherule (diameter≤2mm)

[0044] The Ge Xianmi that forms microsphere among the embodiment 2 carries out expansion culture. The cultivation light is 500-10000 lux, and the cultivation temperature is 15-33°C.

[0045] The concentrations of the various nutrients are as follows:

[0046] MgSO 4 ·7H 2 O: 0-75mg / L; K 2 HPO 4 ·3H 2 O: 0-40mg / L; EDTA·2Na: 0-1mg / L; CaCl 2 2H 2 O: 0-9mg / L; Citric acid: 0-6mg / L; Ferric ammonium citrate: 0-6mg / L; Na 2 CO 3 : 0-20mg / L; H 3 BO 3 : 0-2.86g / L; MnCl 2 4H 2 O: 0-1.81g / L; ZnSO 4 7H2O: 0-222mg / L; Na 2 MoO 4 2H 2 O: 0-39mg / L; CuSO 4 ·5H 2 O: 0-79mg / L; Co(NO 3 ) 2 ·6H 2 O: 0-49mg / L.

[0047] The source of culture water is still purified water, and tap water or clean natural water bodies (such as groundwater, river water, lake water or reservoir water) that meet the national drinking standards can also be selected. When formulating nutrients with culture water sources ...

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Abstract

The invention provides a quick efficient nostoc sphaeroides breeding method which comprises the following steps: (1) soaking nostoc sphaeroides spheres in purified water, carrying out light source induction, and collecting filter liquor when the nostoc sphaeroides spheres are spontaneously broken; (2) mixing the filter liquor with a hormogon culture solution, and performing aerobic culture under the conditions of constant temperature and constant light in a room to obtain microsphere-shaped nostoc sphaeroides; and (3) feeding the microsphere-shaped nostoc sphaeroides as sphere seed sources into next stage for expanded culture, continuously budding from the sphere seed sources by sub-spheres in a culture process and finally dropping to directly form independent microsphere-shaped nostoc sphaeroides. According to the method, the nostoc sphaeroides spheres are broken by adopting the induction process so as to obtain hormogon, the hormogon is cultured to obtain microspheres and finally the budding for breeding is carried out by virtue of the microspheres; for the whole breeding method, the breeding cycle is greatly shortened, the breeding process is simplified, no requirements on the purity of algae seeds are existent, the seed production quantity is large, the economy and practicality are achieved, and large-scale production of the nostoc sphaeroides is facilitated.

Description

technical field [0001] The invention relates to a cultivation technology of cyanobacteria, in particular to a fast and efficient method for breeding kudzu rice. Background technique [0002] Nostoc sphaeroides Kutzing (Nostoc sphaeroides Kutzing) is an edible nitrogen-fixing cyanobacteria. Ge Xianmi is not only nutritious, but also has considerable medicinal value and health care functions. Gexianmi is rich in amino acids, proteins, vitamin C, vitamins B1 and B2, and various minerals. Its calcium content is extremely rare in vegetables, and its polysaccharides have good beauty and skin care effects. According to "Compendium of Materia Medica" and "Supplements to Compendium of Materia Medica", Gexianmi can treat night blindness, rectal prolapse, burns and scalds. In addition, according to the "National Compilation of Chinese Herbal Medicine", Gexianmi is cold in nature and bland in taste, and has medicinal and health effects such as clearing away heat, astringent, nourishin...

Claims

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Application Information

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IPC IPC(8): C12N1/20
Inventor 安彦君王卫周丰易思富熊斌杨胜辉王远忠陈牧
Owner CHANGDE YANDI BIOTECH LTD CO
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