51 results about "Parent body" patented technology

The invention discloses an appreciating landscape pavilion type tree crown modeling method. The method aims to solve the problems that a part of landscape trees are singular in crown type, lack of attractiveness and poor in ornamental value. According to the grafting modeling method, inverse budding is carried out on a center tree, budding is carried out on stand column trees, inverse budding and budding branches are subjected to inarching, and a pavilion type tree crown is formed. A unique model is formed, and artistic and ornamental value of landscape woods can be obviously improved. Natural corners are formed through the inverse budding method, perfect inarching of the corners and grafted sprouts on stand columns is achieved, and the corners and the grafted sprouts grow into a whole. According to the inarching method, contact faces formed by notches are large, formed union faces are large, and trees can easily survive. In addition, the branches after inarching are not completely cut off from parent bodies, the original shapes of parent body roots are maintained, only a part of grafted stems are cut off, then the cut parts of the branches and the cut parts of the grafted stems are tightly combined, and due to the fact that root systems of two trees absorb nutrition, the inarching survival rate is high.

The invention discloses a chaos genetic algorithm based test case intensive simple algorithm. The chaos genetic algorithm based test case intensive simple algorithm comprises initializing male parent body codes; performing fitness calculation on a male parent body; defining genetic operators are defined, wherein the genetic operators comprise three steps of selection, intersection and variation, the genetic variation and the optimization are performed on the male parent body mainly to obtain a new male parent body finally, the change of the variation to an optimal solution can be increased due to the production of the new male parent body, and accordingly the fitness evaluation needs to be performed on the new male parent body after the genetic operators are finished to determine whether the output conditions are met or not, an optimal filial generation is output if yes, and the chaos disturbance is added if not; performing continuous iteration until the difference between fitness average values calculated through twice calculation is less than a preset minimum positive number epsilon 1. According to the chaos genetic algorithm based test case intensive simple algorithm, the algorithm is simple, the test efficiency can be improved, and the test cost can be reduced.

The invention discloses a graft modeling method of branches and ears of trees. At present, crown shapes of part of landscape tree species are single, the beauty is lacked, and the ornamental quality is poor. The grafting in vitro of long branches in the conventional grafting is not easy to survive. In the invention, the tree branches without separating from a parent body are used as the branches and ears, the tree branches of homologous strain or homologous heterologous strain are used as stocks to graft and model. The branches and ears which are used in the invention is the whole branches of the trees but not a branch section, and the branches and ears in grafting is not separated from the parent body, the scion and the stock belong to the homologous strain or the homologous adjacent strain. The grafting healing is easy, the survival rate can reach 97 percent to 100 percent. The graft modeling of the invention is unique and beautiful, the landscape ornamental value of garden landscape tree species is increased significantly. The invention is beneficial to the breeding of endangered rare tree species which are difficult to breed.

The invention discloses a pressure testing device for an adjustable impeller sealing component, which is capable of simulating internal and external pressure environments. The invention aims to provide the pressure testing device which has the advantages of simple structure, convenient operation, small volume, easy adjustment, no need of a travelling crane, low labor intensity and capability of testing the sealing performance of an inverted V-shaped rubber sealing ring in an environment with inside and outside pressures. The pressure testing device comprises a spring (6), a backing ring (7), a middle isolation cover (5), the inverted-V-shaped rubber sealing ring (4) and a pressure ring (3) which are arranged in a pressure testing parent body (2) in turn, wherein an upper blank cap (1) or a lower blank cap (8) is covered respectively to test the external and internal water pressures of the sealing component. The pressure testing device solves the problem that pressure tests are needed outside and inside the sealing component of a large-size adjustable impeller.

The invention discloses a prunus maritima marshall hard branch cutting propagation method, which is carried out according to the following six steps that (1) a cutting seedbed is prepared; (2) root growth promoting agents are prepared; (3) cutting branches are prepared; (4) cutting is carried out; (5) post-cutting management is implemented; (6) domestication is carried out. When being adopted, the method disclosed by the invention avoids the problems of easy germ contamination decay, water loss wilting, low survival rate and the like of cutting slip callus caused by summer cutting, in addition, the damage to parent bodies of the prunus maritima marshall and the potential contamination risk in the cutting process are reduced, the cost is low, the management is simple, and the popularization is easy.

The present invention relates to lightweight components of hybrid design, also termed hybrid component or hollow-chamber lightweight component, composed of a parent body which is composed of surface-pretreated aluminium and which is reinforced by means of thermoplastics and is suitable for the transmission of high mechanical loads, where particular flow aids are added to the thermoplastic in order to improve its physical properties.

The invention discloses a tablet divider which comprises a parent body and a knife body. The tablet divider is characterized in that one end of the knife body is connected with one end of the parent body by an axis, and the other end of the knife body is provided with a knife handle while the middle part of the knife body is provided with a knife edge which is wave-shaped (including zigzag shape, arc and straight line shape), and the other end of the parent body is provided with a tablet passageway, and the parent body is flat infundibulate. When in use, the parent body of the tablet divider is grasped by hands to open the knife body from the knife handle, and the tablet is placed from the position of the passageway of the parent body, and the tablet can be divided into two parts evenly when the knife handle is pressed. As the parent body is flat infundibulate, the tablets can be divided regardless of tablet sizes; as the knife edge is wave-shaped, the tablets can be divided no matter the tablets are flat-shaped or round convex. The tablet divider has the advantages of simple structure, convenient operation, being healthy, standard and fast.

The invention discloses a dionaea muscipula tissue culture and seedling raising method. The method comprises the following steps: (1) preparing a culture medium; (2) preparing an explant; (3) performing the inducing culture; (4) rooting; and (5) performing bagging domestication. By adopting the method, the problems of the traditional sowing multiplication method and leaf cutting multiplication method that the success rate is low, the multiplication efficiency is low and the like can be solved; and when the method of the invention is adopted to perform the tissue culture and seedling raising for the dionaea muscipula, the multiplication coefficient of the culture medium reaches 4 to 5, a batch of culture media can be multiplied only in 4 to 6 months, so that the method has the characteristics of high multiplication coefficient and high multiplication speed; and moreover, the multiplied seedling has the same plant feature with a parent body and is good in resistance. The problem that the dionaea muscipula is difficult to multiply is solved, and the industrialized mass production of the dionaea muscipula can be facilitated.

Provided is a processing method for materials of a foreplate on a rolling mill. Materials of a non-working portion of the foreplate are formed by ordinary medium-carbon low-alloy steel in a pouring mode, a wear-resisting alloy layer is formed on a working portion of the foreplate in a cast-infiltration mode, and the wear-resisting alloy layer is made of a cast steel parent body. The foreplate produced by the processing method for the materials of the foreplate on the rolling mill is simple in production process, the service life of the foreplate is greatly prolonged, and production cost is reduced.

The invention provides a method used for evaluating health of hermatypic coral and based on activity of caspase3. The method includes the steps of: determining a biological index, selecting standard reference points and establishing an evaluation standard, wherein the step of determining the biological index comprises the steps of: selecting the key enzyme caspase3, which is used for responding the environmental stress, of the hermatypic coral, and adopting the activity of the caspase3 of the hermatypic coral as the index for evaluating the health level of the coral, and the step of selectingthe standard reference points comprises that coral samples in ideal growing states and under different stress conditions are selected as the reference points; the step of establishing the evaluation standard comprises that the coral health level standard is established according to data about the activity of the caspase3 of different reference point samples, the growing states of the hermatypic coral and the survival rate of the coral in an growing environment which is restored to an ideal seawater environment. According to the method used for evaluating the health of the hermatypic coral andbased on the activity of the caspase3, the health level of the coral can be evaluated objectively and accurately, and the operation process is simple and fast; besides, not only can the blank of the technology for the investigation of coral resources be filled, but also the selection of a parent body can be guided during artificial transplantation of the coral, so that the research and protectionof the coral reef ecosystem are facilitated.

The invention provides a method for mass propagation of lycoris aurea through somatic embryogenesis. The lycoris aurea tissue culture method for scale production and propagation so as to meet the market demand comprises the steps of A, explant disinfection, B, adventitious bud induction, C, callus induction, D, callus propagation, E callus redifferentiation, F, rooting culture, and H, seedling exercising and transplanting. According to the method, herbicide picloram is applied to tissue culture of lycoris aurea for the first time, stable lycoris aurea tissues capable of being propagated on a large scale are obtained, the callus induction rate reaches 95% or above, and the propagation coefficient of the lycoris aurea is at least increased by 8-10 times. The production cost is reduced, furthermore, the whole fine properties of a parent body can be preserved, and the inheritable character is stable. Through the method, a large number of excellent test-tube seedlings can be formed within a short period of time, large-scale industrialized production is conducted, and a lot of raw materials are provided for ornamental horticulture, pharmacy and other industries as well as the follow-up study of the lycoris aurea.

The invention relates to a method for feeding dermaptera being the natural enemy of tirathaba rufivena walkers. The method comprises the following steps: a fruit fly feeding box is connected with a dermaptera breeding cage through a connecting pipe, and an end cap with a shaft hole is arranged in the connecting pipe; fruit fly adults are put in the fruit fly feeding box, and female and male dermaptera adults are put in the dermaptera breeding cage; when 75 percent of females show a spawning sign, the males are removed from the breeding cage; after eggs are hatched and the females do not protect hewly-hatched nymphs, the females are removed; in the moment, the nymphs are collected and are respectively put in different breeding cages in batches for feeding until adults. The fruit fly feeding box is connected with the dermaptera breeding cage, and meanwhile dermaptera parent bodies to spawn are separated from the males and the parent bodies are separated from the nymphs, so that the behaviors that the dermapteras are mutually impacted and eaten due to human factors are avoided in the breeding process, and the breeding and survival rate of dermaptera is increased. The method provides support for the biological control of tirathaba rufivena walkers and has practical significance.

The invention discloses a forest establishment large-scale planting method for photinia serrulata. The method comprises the following steps that step 1, a greenhouse is adopted for growing seedlings by cutting, the width of a seedbed in the greenhouse is 120-150 cm, and fine sand exposed to the blazing sun for quite a long time is laid on the bottom of the seedbed; a cutting medium layer with thethickness of 20 cm is laid on the fine sand; step 2, high-quality parent body semi-lignified burgeons without diseases and pests are selected and subjected to spike cutting; step 3, cuttage branches after soaking are buried in cutting mediums, and the setting depth is half of the length of each cuttage branch; step 4, after the branches are cultivated and take root in the mediums, the branches aretransplanted after 30-40 days of cultivation; step 5, in the transplanting process, root systems are kept integral; step 6, a humic fertilizer is applied, and then planting pits are dug; step 7, high-quality seedlings are planted in the arranged planting pits; step 8, when the height of the photinia serrulata seedlings reaches 40-50 centimeters, topping is conducted; step 9, after light pruning and shaping, a spherical shape is formed. According to the method, the survival rate is high, the growing period is short, and large-scale planting can be quickly achieved.

The invention discloses an unmanned aerial vehicle-based sea ice thickness detecting system and belongs to the unmanned aerial vehicle technical field. The system comprises a thickness detecting unmanned aerial vehicle, a sea ice thickness detecting device and a ground station; the thickness detecting unmanned aerial vehicle comprises a flight control system arranged in an unmanned aerial vehiclebody; the sea ice thickness detecting device comprises a radar parent body, an adjustable bell mouth and a data transmission module; and the ground station is used for receiving returned electromagnetic wave signals, performing data analysis, calculating and displaying sea ice average thickness within an irradiation range. The unmanned aerial vehicle-based sea ice thickness detecting system of thepresent invention has high accuracy in sea ice thickness measurement; in application conditions such as a remote ocean condition, the system can be carried by a ship, and the ship is made to navigatein safe distances, the measurement system can be remotely controlled to perform sea ice thickness measurement, and high timeliness can be realized; whether remaining power is sufficient under a condition that a measurement distance is long can be predicted through a power prediction module; and early warning can be performed when the remaining power is insufficient, and power insufficiency halfway can be avoided.

The invention discloses a special type oil well pipe with changed materials and structural layers at two ends and manufacturing method thereof, and aims to solve the problems that the oil well pipe leaks and is broken in a well and the service life of the oil well pipe is reduced because the end part of the conventional oil well pipe is easy to abrade eccentrically. In the special type oil well pipe, end pipe bodies are welded at the two ends of an oil well pipe parent body; the inner diameter of each end pipe body is the same as that of the oil well pipe parent body; the wall of the each endpipe body is thicker than the wall of the oil well pipe parent body; and threads are formed on the outer surfaces of the end pipe bodies. The manufacturing method comprises the following steps of: (1) processing a threaded structure; (2) hardening and tempering; (3) performing nitriding treatment; (4) performing oxidation treatment; (5) welding the space between the oil pipe parent body and the end pipe bodies; (6) performing a hydrostatic test; and (7) performing flaw detection. The invention has the advantages that: cost is reduced; the oil well pipe cannot leak and cannot be broken in the well; and the service life of the oil well pipe is prolonged by more than 10 times.

The invention discloses a chrysanthemum planting method. According to the technical scheme, the method sequentially includes the steps of firstly, selecting parent body, and sterilizing and rinsing the parent body; secondly, cultivating at an appropriate position; thirdly, pruning, and performing cuttage; fourthly, performing correction with a correcting agent after seedling recovering of chrysanthemum; fifthly, controlling diseases and pests; sixthly, transplanting. The method has the advantages that invasion of bacteria and viruses into plants can be inhibited, the diseases and pests can be resisted, the chrysanthemum can grow fast, high survival rate is achieved, and economic benefits are increased.

The invention belongs to the field of technologies for preparing medicines, and discloses an estrus promoting powder medicine and a method for preparing the same. The estrus promoting powder medicine comprises, by weight, 6 g of herba epimedii, 6 g of (alcohol-quenched) actinolite, 4 g of radix angelicae sinensis, 6 g of herba leonuri, 5 g of semen cuscutae and 6 g of herba agrimoniae. The herba epimedii, the (alcohol-quenched) actinolite, the radix angelicae sinensis, the herba leonuri, the semen cuscutae and the herba agrimoniae are mixed with one another to obtain the estrus promoting powder medicine. The estrus promoting powder medicine and the method have the advantages that the herba epimedii, the (alcohol-quenched) actinolite, the radix angelicae sinensis, the herba leonuri, the semen cuscutae and the herba agrimoniae are smashed and sieved and are uniformly mixed with one another to obtain estrus promoting powder, good estrus promoting effects can be realized by the estrus promoting powder, the estrus promoting powder is short in action effect time, reproductive development of parent bodies and young bodies can be effectively maintained, the abortion probability can be lowered, and the farrowing rate can be increased.

The invention discloses a method for raising apanteles glomeratus. The method comprises the following steps that preparation work is carried out; brassica campestris is planted in a rearing cage and acollecting cage on a large scale; pieris rapae is caught and then placed in an insectary to be raised, and cabbage worm parent bodies are selected; the cabbage worm parent bodies are moved into the rearing cage to lay eggs, cabbage worms are collected for standby application, and at the moment, the cabbage worms are in a 1-age stage; apanteles glomeratus is reared on a large scale; the cabbage worms in the 1-age stage and pieris rapae pupas are added into the collecting cage to be raised, and cabbage worms in a 2-age stage and a plurality of apanteles glomeratus larvae are obtained after 64-72 hours; the apanteles glomeratus larvae are inoculated after complete eclosion; cabbage worms in a 3-age stage are obtained after 40-48 hours, and adult apanteles glomeratus is taken out; the adult apanteles glomeratus is collected in a new collecting cage; and the adult apanteles glomeratus and the apanteles glomeratus larvae are preserved. The method for raising apanteles glomeratus mainly solves the problem that biological control of insect pests at present pollutes the environment and destroys the ecological balance.

The invention discloses a compressor blade structure, wherein the compressor comprises the following parts: cylinder component with inner space; rotary axle through inner space inserted in the cylinder component; segregation barrier of rotary plug-in function connects rotary axle for segregating the inner space; blade changes the segregated space into intake domain and works linear round-trip movement along the rotary axle of cylinder component; The blade contains certain thickness and area, whose two sides touch the outer rotary axle surface and inner cylinder component separately and plug in the blade parent body plate whose one side is set a rotary plug-in roller to touch segregation barrier line. The invention can move the blade more slippery because of little fiction between blade and segregation barrier, which reduces the noise and lengthen the component lifespan to improve the production reliability.

The invention relates to the field of indoor civil engineering test of a civil engineering subject, in particular to sample preparation equipment of a triaxial sample for simulating overall process erosion. The sample preparation equipment of the triaxial sample for simulating the overall process erosion comprises a pressing mechanism, a sample bearing mechanism, a supporting mechanism, an erosionmechanism, a cutting mechanism, a soil injecting device and a triaxial membrane bearing barrel. The sample bearing mechanism is mounted on the supporting mechanism, cemented soil is injected into thesample bearing mechanism through the soil injecting device, the sample bearing mechanism is placed into the erosion mechanism, the pressing mechanism can cooperate with the sample bearing mechanism to press the cemented soil to obtain a parent body sample, the parent body sample is cut into the standard triaxial sample through the cutting mechanism after being placed into the triaxial membrane bearing barrel, the sample bearing mechanism comprises a sample bearing barrel, annular holes are uniformly distributed in the barrel wall of the sample bearing barrel, and a semipermeable membrane is arranged on the inner wall of a hole of the sample bearing barrel. By adopting the scheme, the problems that an existing high-water content cemented soil triaxial sample is difficult to manufacture, and simulating degree of true force and erodent condition of the cemented soil on site is not high are solved.

The invention discloses a female sturgeon artificial egg collecting method, and belongs to the technical field of aquaculture. The method comprises the following steps that parent fishes are put intoa culture pond with water temperature of 18-25 DEG C, the water temperature is reduced to 7-14 DEG C after 10-20 days and increased to 16-18 DEG C after 12-16 days, and then the parent fishes are cultured for 5-10 days; eggs, with the maturation degree being the IV level, of female parents are selected by adopting abdominal puncture, two times of oxytocin injection are used and matched with an extrusion method to take the eggs, and finally, the mature eggs remaining in the female parent bodies are taken out through living body egg taking. Oxytocin stimulation and extrusion ovulation are performed step by step in the method, so that the eggs with different development degrees in the parent bodies are mature in stages; living body egg taking is used for taking out the remaining mature eggs,so that the number of the mature eggs is increased, and the phenomenon that part of eggs are difficult to take out and become overmature is avoided; the ovulation number of the female parent fishes isincreased while the quality of the eggs is ensured, so that the eggs are fertilized in the optimal mature period, and the number and survival rate of the fertilized eggs are increased.

The invention discloses an inducing and culturing system-establishing method for totipotent stem cell quteASCs of mice or human beings. The materials are early embryo of mice growing for 3-8 days (obtained through in-vitro fertilization or collected from the uterus of a parent body), and the early embryo of the human beings growing for 3-14 days (obtained through in-vitro fertilization or collected from the uterus of a parent body). The inducing method comprises the following two key technical processes: 1, carrying out inducing to establish excess type stem cells ASCs, wherein the key inducing factor is AF; and 2, carrying out further inducing to complete the system establishing technological process of the novel pleuripotent stem cells quteASCs, wherein the key inducing factor is ABCL. According to the invention, through the specific inducing method, the novel stem cells quteASCs of the mice or the human beings are successfully obtained through the specific inducing method, and the totipotency of the novel stem cells quteASCs of the mice or the human beings for developing to the individual tissue of animal or the placenta of the animal is verified through the experimental means.

The invention belongs to the technical field of sampling devices, disclosing a sampling method of a large-diameter granular material undisturbed sample, removing cover rock and soil materials above aprotective layer. After the protective layer is removed, cutting a required columnar rock-soil core sample; after the sample is prepared, the top part and the side surface of the sample are quickly sealed by wax liquid, then the prepared heat-shrinkable or cold-shrinkable reinforcing sleeve is sleeved, and the reinforcing sleeve is shrunk by a corresponding hot air gun or cold air gun to fix the sample, then the root part of the sample is cut to be separated from the parent body; and then reversing, flattening, pouring wax liquid for sealing, using a hot air gun or a cold air gun to shrink thereserved reinforcing sleeve so as to ensure that the sample is completely sealed and closed, then using a fixing device to fix the sample, and finally sleeving damping foam. The method avoids the difficulty of obtaining the mechanical strength parameters of the large-diameter coarse-particle rock-soil body through complex in-situ tests, overcomes the defect of disturbance and damage to the structure of the rock-soil body in the sampling process, and is worthy of popularization and application.

The invention relates to plant breeding methods, in particular to a cyclobalanopsis gilva seedling cutting method. The method includes the following steps that a scion is collected, and the scion is made to be a cutting slip with the length being 8-10 cm, wherein three leaves on the top of the cutting slip are kept, and the 2 / 5 area of each leaf is reserved; an upper notch of the cutting slip is a flat opening, the scion is treated by hormones, the scion is placed in a plug tray in a cuttage mode, sufficient watering is conducted, a sunshade net is built over a cutting bed, the temperature is controlled to be 13-28 DEG C, the relative humidity is kept more than 80%, and water is sprayed once or twice or three times; carbendazim is sprayed weekly. According to the cyclobalanopsis gilva seedling cutting method, cuttage vegetative propagation is adopted, the good characteristic of a parent body can be kept, the rooting percentage of cyclobalanopsis gilva reaches up to 80.4%, the average root length is 3.52 cm, and the average rooting number is 5.55. The survival rate of good seedlings is increased on the premise that the production cycle is shortened, the breeding speed is high, the invested cost is low, operation is easy and convenient, the character is stable, and popularization and large-scale afforestation of the good cyclobalanopsis gilva seedlings can be facilitated.

The invention provides a breeding method for a new tea tree variety. According to the breeding method, variety breeding is carried out for parents with local germplasm resources of Chun'an County, andparent bodies are selected according to high yield, high quality, strong stress resistance, the peculiarity and high whitened degree which serve as the breeding targets; a wild Jiukeng albino tea variety and a group albino tea variety are finally selected as the parent bodies for artificial field interbreeding, and breeding is carried out through early-stage pre-selection identification and systematic breeding; due to the influence of double-parent cell heredity, the characters of offspring obtained by interbreeding tend towards the characters of the wild Jiukeng albino tea variety, in orderto enable the effective characters controlled by plasmagene to be utilized so as to achieve more excellent characters of the wild variety, the parent bodies with the same ecological features, the samegeographic origin and different genetic relationships are selected at the same time, and due to the fact that the genetic bases of the parents are different, a new variety which surpasses the parentsand is high in adaptability can be more easily bred by the obtained new variety.

The invention relates to the technical field of cotton breeding and discloses a high-yield cotton breeding method. According to the high-yield cotton breeding method, a mutant is generated through a mutagenesis method; then the mutant is hybridized with parents with good properties, so that new good properties can be increased in a new variety. According to the embodiment of the invention, the high-yield and disease-resisting new variety is cultured; under the common effect of a single 0.05 to 0.1 percent EMS mutagen, compound type 0.1 to 0.5 percent EMS, a 0.1 to 0.3 percent nitrous acid mutagen and rays, cotton seeds have relatively high distortion rate and distorted plant survival rate; changes in a process from seedlings to plants are observed and distorted plants have a new phenotype,so that the mutagenesis method provided by the invention can be used for effectively improving the mutation rate of the cotton seeds and a foundation is laid for the new phenotype of the variety.

The invention discloses an adjustable cigarette product heating device which comprises a heating body and a controller used for controlling the heating body to carry out heating. The heating body comprises a heating parent body and a sleeve, a containing cavity is formed in the sleeve, the whole sleeve is contained in the heating parent body, the sleeve is provided with at least one row of open holes formed in the axial direction of the sleeve, a ventilation part is arranged on the heating parent body, the relative positions of the open holes and the ventilation part can be adjusted to enable the open holes to be capable of being communicated with the ventilation part, an air inlet is formed in one end of the sleeve, and an air outlet is formed in the other end of the sleeve. By means of the adjustable cigarette product heating device, the ventilation capacity and the eddy state can be adjusted, and therefore people can obtain required smoking senses according to requirements; meanwhile, detection and research workers can conveniently analyze releasing conditions of volatile substance under different eddy states, and physical and chemical changes in the cigarette product heating process are further easily learnt.

The invention provides a novel and high-efficiency tissue culture and rapid propagation method of cymbidium, which is used for realizing large-scale production and propagation to meet market demands. The tissue culture method of the cymbidium comprises the following steps of: A, sterilizing explants; B, inducing callus; C, inducing adventitious buds; D, inducing cluster buds; E, carrying out rooting culture; and F, exercising seedlings and transplanting. The cymbidium can be propagated by the plant tissue culture method, the method can not be influenced by external conditions and can be carried out all the year round, the floor space of nursery stocks can be saved, the production cost can be reduced, all the merits of a parent body can be maintained, and the inheritable character is stable. After the method is used, a mass of good test-tube plantlets can be formed within a short period of time, the large-scale and industrial production can be carried out, and a mass of seedlings can be provided for industries of ornamental horticulture and the like.

The invention discloses a dionaea muscipula tissue culture and seedling raising method. The method comprises the following steps: (1) preparing a culture medium; (2) preparing an explant; (3) performing the inducing culture; (4) rooting; and (5) performing bagging domestication. By adopting the method, the problems of the traditional sowing multiplication method and leaf cutting multiplication method that the success rate is low, the multiplication efficiency is low and the like can be solved; and when the method of the invention is adopted to perform the tissue culture and seedling raising for the dionaea muscipula, the multiplication coefficient of the culture medium reaches 4 to 5, a batch of culture media can be multiplied only in 4 to 6 months, so that the method has the characteristics of high multiplication coefficient and high multiplication speed; and moreover, the multiplied seedling has the same plant feature with a parent body and is good in resistance. The problem that the dionaea muscipula is difficult to multiply is solved, and the industrialized mass production of the dionaea muscipula can be facilitated.

The invention relates to an efficient leaf cutting propagation method for a succulent plant and belongs to the technical field of bioengineering. The efficient leaf cutting propagation method comprises the following main steps: picking off leaves from a parent body, and keeping in a shade place for 2-4 days; testing average volume of the leaves and the average volume, absorbing exogenous liquid, of the leaves, and then calculating concentrations and volumes of a plant hormone 6-BA and NAA which are required to be prepared, so that concentrations of 6-BA and NAA in the leaves are 2-3mg / L and 0.1-0.2mg / L respectively after injection; preparing 6-BA and NAA with appropriate concentrations, and injecting to the middle parts of the leaves; and putting the leaves on a mixed medium to be cultured. The efficient leaf cutting propagation method provided by the invention can enable the leaves of the succulent plant to keep higher plant hormone level, and propagation coefficient can be improved by 3-8 times compared with that of the conventional method; meanwhile, operation is simple, and hormone utilization rate is high, so that the efficient leaf cutting propagation method has great popularization potential.