670 results about "Nitrous acid" patented technology

The present invention belongs to an industrialization aquiculture field, particularly relates to a closed cycle water industrialized fish cultivating system. The system separates solid and liquid in a storage sedimentation basin and a backwater channel sedimentation and rotational flow pool sedimentation, the tiny particulate matter is separated and purified by a sandrock filtering bed of a one level biological filter based on the water particulate matter separation, the water purified by the one level biological filter is feed into a two stage biological filter, the biological film of biologic filling material in the one level and the two stage biological filters is degraded with ammonia nitrogen and azote nitrous acid, the total purifying rate of the water particulate matter reaches more than 99%, the culturing water is added with oxygen mechanically or by liquid oxygen, finally the water is disinfected and sterilized through a ultraviolet ray sterilizer, and the clean ecological water is feed into a fish pool, thus the waste water of the fish pool may be purified and used in repeat. The fish-cultivating system implements completely closed cycle water industrialized fish cultivation, the fish is out of a disease or less, and the fish survival is more than 80%.

The invention discloses a method for quickly starting a completely autotrophic nitrogen removal over nitrite process on sewage on the conditions of constant temperature and low ammonia nitrogen and belongs to the field of city sewage treatment and reutilization. The completely autotrophic nitrogen removal over nitrite (CANON) process is started in a biological filter tank reactor and comprises the following steps: firstly, nitrifying sludge by using an inoculating part, and performing aerobic nitrification start with sufficient oxygen supply to build a nitrosobacteria-dominated and nitrobacterium-dominated microorganism system; inhibiting the growth of nitrobacterium through intermittent aeration / anaerobism and enriching anaerobic ammonium oxidation bacteria; and finally, performing continuous aeration, controlling ammonia nitrogen to be oxidized to a stage of nitrous acid through restrictive oxygen supply, and optimizing microenvironment where nitrosobacteria and anaerobic ammonium oxidation bacteria coexist so as to successfully start the CANON process. By using the method, the problem that the growth enrichment of the anaerobic ammonium oxidation bacteria is slower for a long term can be solved; moreover, the starting way is simple and feasible, and the difficulty of the start of a single-stage autotrophic nitrogen removal system is reduced; and therefore, a method is provided to start the CANON process on stimulated waste water on the conditions of constant temperature and low ammonia nitrogen.

The invention relates to a bacteria strain for short-cut denitrification and an application thereof. The short-cut denitrification bacteria strain is arthrobacter creatinolyticus FDN-1 and is preserved in Ordinary Microorganism Center of China Committee for Culture Collection of Microorganisms on March 11, 2010, and the preservation number is CGMCC No. 3657. The bacteria strain can directly adopt nitrite nitrogen as a substrate to complete the short-cut denitrification process. When the arthrobacter creatinolyticus FDN-1 is used for processing ammonia-contained waste water, the technique is simple, high denitrification activity can be still obtained under the condition that low-concentration organic carbon source exists, and at the same time the high-concentration organic carbon source can be withstood. When the bacteria strain is in use, the system is rapid to start, and a vast application prospect can be realized in the denitrification treatment process of different types of waste water.

The present invention discloses a short-cut nitrification-denitrification microbial agent. The microbial agent contains Arthrobacter creatinolyticus FDN 1, Flavobacterium mizutaii FDN 2, Paracoccus denitrificars DN 3 and Methylobacterium phyllosphaerae SDN3. The preservation register numbers of the four strains respectively are CGMCC No.3657, CGMCC No.3659, CGMCC No.3658 and CGMCC No.3660. With the microbial agent, the ammonia nitrogen removing, the total nitrogen removing and the CODcr removing in the same reactor can be achieved, the wastewater treatment effect is good, and the microbial agent is especially suitable for the purification treatment of the wastewater containing the ammonia nitrogen so as to really achieve the short-cut nitrification-denitrification or simultaneous nitrification and denitrification.

The invention discloses a making method of C1-C4 alkyl ester of nitrous acid, which comprises the following steps: doing the oxidizing reaction and esterifying reaction in two reactors separately; doing the oxidizing reaction in the oxidizing reactor; doing the esterifying reaction in the esterifying tower reactor; inputting nitrogen oxide, oxygen and one or more of inert gas into oxidizing reactor; selecting the nitrogen oxide from one or more composite gas of NO, N2O3, NO2 and N2O4 with NO constantly; making the mole of NO more than the mole of NO2; using 0.15-0.3 m oxygen for each NO m; making the bulk of inert gas at 0-90% corresponding to total gas; inputting the oxidizing reacting product into the esterifying tower reactor to react with C1-C4 alkanol; using 0.8-3.0 m alkanol for each gram atom nitrogen. The invention can transfer heat effectively to reduce by-product, which is smaller than traditional equipment.

The invention discloses an aspergillusniger strain for producing beta-glucuroide. Aspergillusniger T2 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on 26th September, 2008 with the preservation number of CGMCC No.2715. The aspergillusniger T2 strain is the aspergillusniger strain which is obtained by performing mutagenesis with ultraviolet rays and nitrous acid and screening by using a Congo red-carboxymethylcellulose (CMC) double-layer flat plate, has high enzymatic activity and is used for producing the beta-glucuroide. The aspergillusniger T2 strain is applied to the production of the beta-glucuroide. The beta-glucuroide produced by performing liquid state fermentation on corn cob, yeast powder and the like serving as raw materials and the aspergillusniger T2 strain serving as the strain has the characteristics of enzymatic activity, simple production method and the like.

A catalyst for gas-phase synthesis of dimethyl (or diethyl) oxalate from CO and nitrite is prepared from alpha-Al2O3 as carrier and Ce and Pd as active components through the dipping method. Its advantages are high reaction activity and selectivity, long service life and easy control of reaction.

The invention relates to a high-efficiency and low-consumption recycling and treating method for urban sewage, belonging to the technical field of treating and reusing the urban sewage, which aims to solve the problem of poor effects of dephosphorization and denitrogenation in prior art. The recycling and treating method in the invention is characterized in that: first, biological phosphorus removal is conducted for the pretreated sewage, meanwhile, the organic substance is also removed; then, the biological autotrophic denitrogenation is carried out, thus completing the recycle and treatment of the sewage. The biological autotrophic denitrogenation can be implemented in six ways: first, the sewage with the phosphorus removed biologically enters a nitrous acid biofilter, and then enters an ANAMMOX activated sludge reactor; second, the sewage enters a nitrous acid activated sludge reactor and subsequently enters an ANAMMOX biofilter; third, the sewage enters the nitrous acid biofilter, and then enters the ANAMMOX biofilter; fourth, the sewage enters the nitrous acid activated sludge reactor before entering the ANAMMOX activated sludge reactor; fifth, the sewage enters a single-segment biofilter reactor; and sixth, the sewage enters a single segment activated sludge reactor. The recycling and treating method for urban sewage in the invention has the advantages of good effect of sewage treatment, and low treatment costs.

A process for preparing the oligose of fucosan sulfate includes such steps as preparing the aqueous solution of fucosan sulfate, adding oxidant chosen from hydrogen peroxide, hypochlorous acid, nitrous acid, or their salts, heating, membrane ultrafilter, vacuum concentrating and freeze drying. Its advantages are high medical effect to protect liver and take care of health, and low cost.

The invention discloses a nitrite bacterium culture promoter, which consists of metal salts, polyamine substances, organic acid hydroxylamine and Na2SO3, wherein in parts by weight, the content of the metal salts is 40-100 parts by weight, preferably 50-80 parts by weight; the content of the polyamine substances is 5-30 parts by weight, preferably 10-20 parts by weight; the content of the organic acid hydroxylamine is 0.05-1.5 parts by weight, preferably 0.1-1.0 part by weight; and the content of the Na2SO3 is 10-40 parts by weight, preferably 20-30 parts by weight; the metal salts include calcium salt, ferrous salt and copper salt; and the molar ratio of Ca<2+> to Fe<2+> to Cu<2+> is (5-15) to (1-8) to (0.5-5), and preferably (8-12) to (2-6) to (1-4). The promoter is simple in formula and easy for preparing; and the promoter can be applied to the culture process of the nitrite bacteria and the promoter can be also directly added to a sewage treatment system, so that the starting of partial nitrification-denitrification and partial nitrification-anaerobic ammonia oxidation processes is accelerated and the stable running of the processes is achieved.

The invention belongs to the environmental microorganism field, relates to a high efficiency heterotrophic nitration aerobic denitrifying bacteria and the culture method and the purpose. The bacterium is Pseudomonas putida DN1.2 with the preservation and registration number of CCMCC M207075, which can effectively remove the ammonia nitrogen, nitrous acid and the mixture in water. At the same time, the bacterium also can remove the CODCr in the organic waste water. The bacterium is suitable for being used in the treatment of organic nitrogenous effluent with high concentration and inorganic nitrogenous effluent. The nitrite and nitrate can not be produced in the denitrification process. The waste water treatment process is simple by using the strain and the denitrification effect is stable.

The invention relates to a method for starting a single-stage self-supported denitrification reactor, comprising the following steps: establishing a microflora with nitrosobacteria and nitrobacterium as the mainstay under the conditions of restricted supply of oxygen and small amount of available organic carbon source; then controlling ammonia nitrogen oxidization to nitrous acid phase through restricted oxygen supply so as to gather nitrosobacteria, inhibit growth of nitrobacterium and establish the microflora with the nitrosobacteria as the mainstay; optimizing the microenvironment where nitrosobacteria and anammox coexist by adjusting aeration modes and controlling dissolved oxygen level on the basis of the stable nitrous acid system, so as to promote growth and enrichment of the anammox and successfully start the single-stage self-supported denitrification reactor. The method of the invention solves the problem of inoculum source of the single-stage self-supported denitrification reactor, features simple and practical starting mode, reduced difficulty of starting the single-stage self-supported denitrification reactor and shortens times for starting the single-stage self-supported denitrification reactor.

The invention discloses a method of eliminating nitrous acid alkyl ester and nitrogen oxide gas in discharged gas, comprising following procedures. a. discharged gas is sent into the recovery tower of nitrous acid alkyl ester and alkyl alcohol is used to adsorb nitrous acid alkyl ester in discharged gas so as to obtain alkyl alcohol solution of nitrous acid alkyl ester. b. discharged gas treated by the procedure a is sent into catalytic reduction reactor so that not-adsorbed nitrous acid alkyl ester and nitrogen oxide gas are reacted with reduction gas to form N2 under the action of load catalyst; the temperature for catalytic reduction reaction is controlled in 200-600deg.C and the pressure is controlled in 0.05MPa-1MPa; the cubage proportion of reduction gas and nitrogen oxide gas is more than 1:1. The invention not only adsorbs and recovers nitrous acid alkyl ester but also transforms nitrogen oxide gas in to nitrogen by catalytic reduction reaction. The total concentration of nitrogen oxide gas and nitrous acid alkyl ester in treated discharged gas is lower than 200ppm.

A process for the preparation of a catalyst which is highly active for the synthesis of hydrocarbons from mixtures of hydrogen and carbon monoxide. A silica or silica-containing support is treated with a solution containing both an Iron Group metal, or metals, and nitrous acid, nitric acid, or a nitro-containing organo, or nitro-containing hydrocarbyl compound, or compounds, sufficient to hydroxylate the surface thereof and increase the number of hydroxyl groups on the surface of the support such that the metal component will be highly dispersed, this increasing the activity of the catalyst in a hydrocarbon synthesis reaction as contrasted with that of a catalyst of similar composition, similarly prepared except that the support component of the catalyst was not contacted and simultaneously treated with both the Iron Group metal and the acid.

The invention relates to a method for culturing shortcut nitrification granular sludge by combining the dual inhibition of free ammonia (FA) and free nitrous acid (FNA), and belongs to the technical field of the biological denitrification of wastewater. The method comprises the following steps of: inoculating flocculent active sludge to a sequencing batch reactor (SBR), and pumping wastewater which contains chemical oxygen demand (COD) and ammonia nitrogen and serves as inflow from the bottom of the reactor; after a liquid level is reached, starting a stirrer and a blast blower to perform aerobic nitration, degrading organic matters and oxidizing the ammonia nitrogen to form nitrites; after the nitration is finished, stopping aerating and stirring, precipitating to obtain supernatant containing the nitrites, and discharging sewage from a drainage valve, wherein in the culture conditions, the temperature of the reactor is between 25 and 30 DEG C, NaHCO3 solution is added after aeration is performed for 30 minutes, so that the pH value is kept between 7.5 and 8.0, dissolved oxygen is between 3 and 5 mg / L, upflow is provided at the speed of between 1.0 and 1.5 cm / s, and sludge age is between 12 and 15 days; and culturing for 30 to 50 days to obtain the shortcut nitrification granular sludge. By the method, the removal efficiency of a denitrification system can be improved effectively, oxygen supply and denitrification carbon sources can be saved, the yield of the sludge can be reduced, the volume of the reactor can be reduced, and the construction and operating cost can be saved.

Provided is a biological nitrogen removal method which includes, causing to flow a support having, on the surface portion thereof, a two-layered microbial film which holes, in the outer layer, nitrite type nitrifying bacteria or aerobic bacteria and nitrite type nitrifying bacteria as a dominant species and, in the inner layer, anaerobic ammonia oxidizing bacteria as a dominant species while being surrounded with the nitrite type nitrifying bacteria; and thereby carrying out denitrification of the water to be treated by making use of the anaerobic ammonia oxidation reaction. In the biological nitrogen removal method, a feed rate of the support (total surface area of the support per unit capacity of the reaction tank) is adjusted so that an amount of nitrous acid produced by the nitrite type nitrification reaction through the action of the nitrite type nitrifying bacteria reaches a level to inhibit a nitrate type nitrification reaction.

The invention discloses a microbial preparation for improving an aquatic product culture environment and a preparation method thereof. A strain with optimal nitrite degradation effects is screened and separated from a mud sample in Tongan and Haicang ports of Xiamen city of the Fujian province, another strain with optimal nitrite degradation effects is screened and separated from a mud sample in the intertidal zone of Ningde city of the Fujian province, and the two strains respectively are Rhodopseudomonas palustris PSB20 and Bacillus subtilis ND04. The microbial preparation prepared from the two strains can degrade ammonia nitrogen and nitrous acid in an aquatic product culture environment, has good decomposition effects on residual bait organic matters, has a certain effect of inhibiting pathogenic vibrio bacterium growth in culture water, and has a good production application prospect. The microbial preparation has the characteristic of simple preparation processes.

The present invention relates generally to compositions and methods for the use of nitrous acid solutions to disinfect inanimate surfaces and animal tissues, and to treat diseases and wounds. More specifically, the invention deals with the partial and selected conversion of nitrite ion to nitrous acid in order to optimize the germicidal efficacy and duration of the nitrous acid consistent with the nature of the intended application.

The invention relates to metal bearing water-based cleaning agent, rinsing agent matched with the water-based cleaning agent, and the preparation method thereof. Petroleum sodium sulfonate, triethanol amine oleate, fatty alcohol-polyoxyethylene ether, emulgator, and cocoanut oil diethanolamine phosphoric acid ester are mixed with tiethanolamine and ethanolamine according to the weight percentage, are stirred uniformly at room temperature, then are mixed with other raw materials and water, then antibubbling agent AF9020 is added, and therefore the brown yellow and transparent concentrated cleaning agent is formed; nitrous acid dicyclohexylamine, oleic acid triethanolamine and petroleum sodium sulfonate are respectively dissolved into the mixed solvent of tiethanolamine and mono ethanolamine, then benzotriazole is dissolved into water to mix the two solvent, to form the brown yellow and transparent concentrated rinsing agent; the cleaning agent and the rinsing agent are mixed into 5 percent treatment fluid and 1 percent of treatment fluid, then the treatment fluid is cleaned for 30 seconds to 1 minute under ultrasonic wave, rinsed for 30 seconds, and then hot air seasoning, drying and 48 h rust preventing are performed. The invention can replace gasoline cleaning agent, kerosene cleaning agent, and universal water-based cleaning agent, the used raw material is easy to be acquired, the production cost is low, and the invention without parkerizing process can effectively prevent the environmental pollution.

The invention relates to a multi-project detector for detecting the security of food. The operational power supply separately provides the power to a light path part, a monolithic processor and an auxiliary circuit part. The monolithic processor is separately connected with the light path part, the auxiliary circuit part, an input part and an output part. And the monolithic processor and the auxiliary circuit part are used for calculating and controlling, the input part and the output part are used to input and output the information. The invention can detect the chemical substances which have harm to human body like agricultural chemicals, sulphur dioxide, nitrous acid, rongalite lump, hydrogen peroxide, methyl alcohol, dairy product protein, nitrate and heavy metal, the detecting result is accurate, the detecting speed is quick, the density can be directly showed, the man-machine contact surface is friendly, and the invention has the functions of data storage, inquiry, result printing and GPRS wireless transmission to data server. The invention can be used in real-time, and has the advantages of high detecting efficiency and high automaticity, convenient carrying, practical using, low cost and good effect.

This invention discloses a metal cleaning agent with high effect and multi-functions, which uses phosphoric acid and zinc oxide as A material, water, tripoly phosphate, thiourea, and uses tartaric acid, C6H12N4, butanone, thiocyanic acid natrium, triethanolamine, fluorin sodium metasilicate, hydrofluoric acid, lemon acid, nitrous acid natrium, op-10, JFC and alcohol as B material, the main character is that after A material is mixed by a certain weight ratio, it combines B material with a certain weight ratio. This invention combines oil removing, dirty removing, rust preventing, deactivation, inhibiting, phosphate 8 in 1 functions together, which has well cleaning effect, and the work surface quality is high, the dealing technology is simple, the cost is lower.

The invention discloses a denitrification method by sewage short-cut simultaneous nitrification and denitrification, comprising the following step of: adding a nitrous acid denitrifying inoculant into sewage containing ammonia and nitrogen, wherein the ammonia-nitrogen-containing sewage treatment temperature is 18-40 DEG C, dissolved oxygen is 0.1-3mg / L, and pH is 7-9. According to the method, the heterotrophic nitrobacteria are used for advantage combination as the enhanced microorganism to process wastewater, thus realizing the removal of ammonia nitrogen, total nitrogen and CODcr in a samereactor. The effect of wastewater treatment is good. The method provided by the invention is especially suitable for the purification treatment of wastewater containing ammonia nitrogen, and can be used to actually realize denitrification by short-cut nitrification and denitrification or simultaneous nitrification and denitrification.

The invention relates to an urban sewage treatment device and method for quick realization of partial nitrification based on free nitrous acid inhibition and belongs to the field of sewage treatment. The method comprises the steps that an FNA treatment unit is arranged in parallel in the sludge backflow process of a traditional A2 / O technology secondary sedimentation tank, the growth activity of nitrite oxidizing bacteria (NOB) is partially inhibited by using the selective bacterium inhibiting effect of FNA on ammonia oxidizing bacteria (AOB) and NOB under the anoxic condition, sludge then flows back to a main flow A2 / O reactor, NOB in active sludge are gradually elutriated under the effect of sludge age control, and thus partial nitrification of the urban sewage is quickly achieved in the main flow A2 / O reactor. The method is simple, heating or real-time control is not needed, and a good sedimentation effect is achieved; in addition, phosphate removal and nitrogen removal are carried out simultaneously, the phosphate removal effect is not affected by addition of the FNA treatment unit, the efficient and simultaneous phosphate removal and nitrogen removal effect of the low-carbon source urban sewage is achieved, and the urban sewage treatment device and method for quick realization of partial nitrification based on free nitrous acid inhibition are suitable for upgrading transformation of existing urban sewage plants.

The invention relates to a method and device for realizing the lasting and stable accumulation of nitrite nitrogen under a low-temperature condition. The lasting and stable accumulation of the nitrite nitrogen is completed in two stages of biological aerated filter systems. The method comprises the following steps: step a. starting a first-stage biological aerated filter and a secondary-stage biological aerated filter, cleaning a filter material, soaking, forming a biofilm, commissioning and operating; b. preparing testing wastewater, and maintaining water temperature at 6 to 10 DEG C; c. enabling wastewater to flow out from a head tank and enter the first-stage biological aerated filter from below; and d. enabling yielding water of the first-stage biological aerated filter to enter the secondary-stage biological aerated filter from bottom to top in the same way. The nitration types of the two stages of biological aerated filter are nitrous acid-type nitration, the stable accumulation of NO2--N in the yielding water is shown, the accumulation rate is 63.2% to 79.8%, and the accumulation time is up to 30 to 35 days. The method has the advantages that the biofilm process adopting the two stages of biological aerated filters can be used for solving the problems of high energy consumption at a high temperature (30 to 40 DEG C), and sludge bulking under a low DO (Dissolved Oxygen) condition, and the effective removal of organic matters in wastewater with a low C / N ratio (less than 3) and the lasting and stable accumulation of NO2--N are realized.

The invention relates to a biology preparation for reducing nitrosamine content in tobacco, and the manufacturing method and application, which belongs to biology technology field. The fungus Pseudomonas aeruginosa KenLXP30 product in the invention is preserved in normal microbe centre of china microbe preservation management committee in Jul 28 2003, the preserving number is CCTCC NO.0985. The invention picks out a fungus KenLXP30 in large amount of tobacco inner bacteria which can eliminates the amino nitrous acid content in tobacco, produces the biology preparation which can reduce the hard of tobacco. The preparation can reduce the amino nitrous acid content in white rib tobacco prominently, and it has good application value in tobacco industry.

The invention discloses a method and a device for removing nitrate in aquaculture seawater through heterotrophism and autotrophy series connection denitrification. The device comprises a heterotrophic organism denitrification unit, an autotrophic organism denitrification unit and an outlet water filtering unit, wherein the heterotrophic organism denitrification unit comprises a heterotrophic denitrification bioreactor; the autotrophic organism denitrification unit comprises an autotrophic denitrification bioreactor; the outlet water filtering unit comprises a solid filtering device; aquaculture seawater to be treated is sequentially introduced into the heterotrophic denitrification bioreactor, the autotrophic denitrification bioreactor and the solid filtering device, and the removal of nitrate in the aquaculture seawater can be realized. The method and the device have the advantages that on the premise of maintaining high-concentration dissolved oxygen of inlet water, the removal of nitrate in circulation aquaculture seawater is realized; the generation of nitrous acid in the outlet water is strictly controlled; the consumption of organic carbon sources and the generation quantity of sulfate radicals are reduced; the treatment cost is reduced.

A lithium secondary cell which comprises a positive electrode and a negative electrode which have a specific composition and specific properties and a nonaqueous electrolytic solution containing a cyclic siloxane compound represented by the formula (1), a fluorosilane compound represented by the formula (2), a compound represented by the formula (3), a compound having an S-F bond, a nitric acid salt, a nitrous acid salt, a monofluorophosphoric acid salt, a difluorophosphoric acid salt, an acetic acid salt, or a propionic acid salt in an amount of at least 10 ppm of the whole nonaqueous electrolytic solution. The lithium secondary cell has a high capacity, long life, and high output. In the general formula (1), R<1> and R<2> each is a C1-12 organic group; and n is an integer of 3-10. In the general formula (2), R<3> to R<5> each is a C1-12 organic group; x is an integer of 1-3; and p, q, and r each is an integer of 0-3, provided that 1=p+q+r=3. In the general formula (3), R<6> to R<8> each is a C1-12 organic group; and A is a group constituted of H, C, N, O, F, S, Si, and / or P.

The invention provides a comprehensive utilization method of aromatic nitro compound chlorination tail gas, which is characterized in that: sulfuric acid is used for absorbing nitrogen oxides and nitryl chloride in the aromatic nitro compound chlorination tail gas to generate mixed acid containing nitric acid, sulfuric acid and nitrous acid, the generated mixed acid is used for nitration of aromatic compound; the tail gas which is not completely absorbed by sulfuric acid is absorbed by water to form acid liquor comprising hydrochloric acid mainly and a little nitric acid; the remaining tail gas is processed by dilute base to be absorbed completely, and waste liquor enters wastewater treatment system.

The invention discloses a biological denitrification process of organic nitrogen waste water, and belongs to the technical field of waste water denitrification. The biological denitrification process comprises the following steps: (1) pre-treating the organic matter waste water, and then sending the pretreated organic matter waste water into an anaerobic fluidized bed at a given speed; (2) facilitating the waste water to flow by the anaerobic fluidized bed having heterotrophic bacteria, converting organic nitrogen to be ammonia nitrogen, and collecting the rest organic matters by utilizing a membrane; (3) enabling the waste water to enter a two-layer micro-organism membrane reaction tank, carrying out nitrous-acid nitration reaction by virtue of a first layer of microorganism membrane with nitrous-acid nitration bacteria, to generate nitrous acid; and carrying out the denitrification by virtue of a second layer of microorganism membrane with anaerobic ammoxidation bacteria. The entire process flow is reasonable in design, the operation condition is easy to implement, the cost is low, the denitrification rate is extremely high, and the biological denitrification process is suitable for continuous mass treatment on the organic matter waste water.

The invention relates to a method for facilitating formation of microbial floccules in a freshwater aquiculture pond and aims at solving the problems that in the freshwater aquiculture pond, nitrogen sources of ammoniacal nitrogen, nitrous acid nitrogen and the like are excess, carbon sources are not enough, the pond water quality is poor and survival rate of cultured fishes is low. The method comprises the steps of calculating the quantity of cane molasses added into the freshwater aquiculture pond for each time according to general formula delta CH = 35*H*S*CNH3-N, wherein the delta CH represents the quantity of added cane molasses, the H represents the depth of a pond water body, the S represents the area of the pond water body, and the CNH3-N represents ammonia nitrogen content in the pond water body; adding the water in the freshwater aquiculture pond into the cane molasses to dilute the cane molasses and then splashing diluted cane molasses into the whole pond evenly, namely accomplishing the method for facilitating the formation of the microbial floccules in the freshwater aquiculture pond. By means of the method, forming quantity of the microbial floccules can be improved, non-pathogenic heterotrophicy bacteria can be stimulated to reproduce in a large scale, thereby restraining reproduction of pathogenic bacteria and improving the pond water quality.