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163results about How to "Realize accumulation" patented technology

Single-channel synthetic aperture radar moving-target detection method based on multi-apparent subimage paire

A method for detecting radar mobile object of single channel synthetic hole diameter based on multivision sub image pair includes forming multiple symmetric sub image pairs by multivision image of synthetic hole diameter radar data, forming each sub image pair by two subvision images, utilizing subvision image cancellation to accumulate cancellation result by two - dimensional united means along position and distance directions for effectively suppressing background clutter interference as well as for realizing energy accumulation of mobile object then applying constant false alarm technique to finalize detection on mobile object.
Owner:TSINGHUA UNIV

Task planning common platform based on satellite-ground resource model

The invention discloses a task planning common platform based on a satellite-ground resource model. Through the configuration of the satellite-ground resource model, the platform is suitable for the type, the characteristics, the working mode and the use constraints of each satellite; for a specific business rule and a satellite use constraint, a special plugin is expanded through the personalized customization of a contract plugin interface; and through the integration of a universal plugin and the special plugin as well as the custom expansion of a personalized application, various types of task planning application software which meet each application type and each satellite use requirement are created, functions that multi-satellite joint planning is carried out, each satellite observation task is subjected to optimal distribution and observation mode optimization, each receiving resource task is subjected to optimal distribution and the like are realized, intersatellite task conflicts, satellite sensor task conflicts and data receiving conflicts are eliminated, a task planning scheme is generated, and visual deduction display is carried out. By use of the platform, a "platform+plugin" technology implementation way is adopted to realize task planning generality basic functions and frame functions, and exhibits high universality, adaptation and expandability.
Owner:NO 54 INST OF CHINA ELECTRONICS SCI & TECH GRP

Thyroid tumor ultrasonic image recognition method and device

The invention discloses a thyroid tumor ultrasonic image recognition method and device, and the method comprises the steps: selecting a tumor region in a thyroid tumor ultrasonic image, extending a certain boundary scope and then performing cutting, carrying out the benign and malignant marking, and enabling the cut images to form a training set; training a selected CNN (Convolutional Neural Network) through the training set, and forming a thyroid tumor ultrasonic image recognition model; obtaining a to-be-recognized thyroid tumor ultrasonic image, selecting a tumor region and extending a certain boundary scope, and carrying out the benign and malignant recognition through the thyroid tumor ultrasonic image recognition model. The method and device are used for assisting a doctor to diagnose the benign and malignant thyroid tumors, obtain the accuracy of 90% or greater in the detection test of the benign and malignant thyroid tumors through the thyroid tumor ultrasonic image, and is ofgreat reference significance to the actual clinical diagnosis.
Owner:FUDAN UNIV SHANGHAI CANCER CENT +1

Solid-state imaging device and method of manufacturing the same

The MOS type solid-state imaging device has plural pixels each of which comprises a photo-diode and a MOS transistor on a substrate. A gate electrode is formed on the channel dope layer formed in the surface of the p-type well layer. By ion implantation of n-type impurity ions via the gate electrode as the mask, the n-type source region and the drain region are formed in the region corresponding to the MOS transistor, and the n-type impurity region is also formed in the region corresponding to the photo-diode. In the well layer, a high impurity density region as a hole pocket is self-aligned to the gate electrode.
Owner:INNOTECH CORP

General fault detecting and maintenance method for equipment

The invention discloses a general fault detecting and maintenance method for equipment. The general fault detecting and maintenance method for the equipment comprises the following steps of carrying out modeling on maintenance knowledge of the equipment faults, automatically detecting and diagnosing the faults and carrying out auxiliary maintenance based on a model, simulating maintenance training based on the model, optimizing an experience knowledge model in real time in the maintenance training process, and synchronizing maintenance information and experience knowledge model information in the maintenance training process. Compared with an existing equipment maintenance method, the general fault detecting and maintenance method for the equipment enables the accumulation and the sharing of equipment maintenance experience knowledge and the automatic diagnosis of equipment faults to be achieved, and assistance can be provided for related maintenance strategies through the analysis and the exploitation of the maintenance record information. In addition, effective transmission of the experience of experts to primary maintainers is achieved through the step of simulation training. The general fault detecting and maintenance method for the equipment is especially suitable for chemical defense troops and other occasions where a large amount of complex equipment needs to be maintained frequently and where the turnover rate of maintenance personnel is high. Practice proves that the general fault detecting and maintenance method for the equipment can obvious improve equipment maintenance efficiency, promote maintenance levels and improve maintenance quality.
Owner:NANJING UNIV +1

Method and device for realizing lasting and stable accumulation of nitrite nitrogen under low-temperature condition

The invention relates to a method and device for realizing the lasting and stable accumulation of nitrite nitrogen under a low-temperature condition. The lasting and stable accumulation of the nitrite nitrogen is completed in two stages of biological aerated filter systems. The method comprises the following steps: step a. starting a first-stage biological aerated filter and a secondary-stage biological aerated filter, cleaning a filter material, soaking, forming a biofilm, commissioning and operating; b. preparing testing wastewater, and maintaining water temperature at 6 to 10 DEG C; c. enabling wastewater to flow out from a head tank and enter the first-stage biological aerated filter from below; and d. enabling yielding water of the first-stage biological aerated filter to enter the secondary-stage biological aerated filter from bottom to top in the same way. The nitration types of the two stages of biological aerated filter are nitrous acid-type nitration, the stable accumulation of NO2--N in the yielding water is shown, the accumulation rate is 63.2% to 79.8%, and the accumulation time is up to 30 to 35 days. The method has the advantages that the biofilm process adopting the two stages of biological aerated filters can be used for solving the problems of high energy consumption at a high temperature (30 to 40 DEG C), and sludge bulking under a low DO (Dissolved Oxygen) condition, and the effective removal of organic matters in wastewater with a low C / N ratio (less than 3) and the lasting and stable accumulation of NO2--N are realized.
Owner:HUBEI POLYTECHNIC UNIV

Two-dimensional multi-aperture scan synthetic aperture radar (SAR) imaging method

The invention discloses a two-dimensional multi-aperture scan synthetic aperture radar (SAR) imaging method. The method includes the following steps of S101, subjecting echo data received by sub-apertures to range direction pulse compression respectively by using a range direction matched filter; S102, uniting the echo data which are subjected to pulse compression in a range direction and an azimuth direction to form a matrix Z and subjecting rows of the matrix Z to weighting summation by using a two-dimensional weighting vector wk respectively; and S103, subjecting the echo data to focusing imaging by using an extended chirp scaling (ECS) algorithm. By means of range direction and azimuth direction united two-dimensional digital beam forming (DBF) processing, echo separation of various sub-swaths and frequency spectrum reconfiguration of azimuth periodicity non-uniform sampling signals are achieved, and problems of mutual interference and azimuth multi-channel non-uniform sampling of echo signals of various sub-swaths under a two-dimensional multi-aperture scan SAR mode are solved.
Owner:INNER MONGOLIA UNIV OF TECH

Satellite data receiving task scheduling system based on OSGI (Open Service Gateway Initiative) and workflow and satellite data receiving task scheduling method based on OSGI and workflow

InactiveCN104239143AImprove scalabilityReduce the complexity of logical interpretation processingProgram initiation/switchingExtensibilityTask completion
The invention discloses a satellite data receiving task scheduling system and a satellite data receiving task scheduling method. The system comprises a data receiving and recording task processing device, a data transmission task processing device, a quick-look task processing device, a filing task processing device, a quality monitoring task processing device, a record transmission task processing device, a transmission and retransmission task processing device, an operation flow monitoring device, a satellite receiving project management device, a satellite receiving project resolving processing device, a satellite receiving project monitoring device, a satellite receiving report processing device, a task completion checking device, a task planning device, a task checking device and a manual closed loop processing device. The system can be used for overcoming the defects of low development efficiency, poor system maintenance performance, poor expandability and the like of an original system, realizing system capitalization and enhancing the system expandability and the maintainability.
Owner:INST OF REMOTE SENSING & DIGITAL EARTH CHINESE ACADEMY OF SCI +1

Identification method and device for training thyroid tumor ultrasonic image on line

The invention discloses an identification method and device for training a thyroid tumor ultrasonic image on line. The method comprises the steps that a set of ultrasonic images are acquired, a tumorarea is selected from the ultrasonic images, a certain edge range of the tumor area is amplified for cutting, benign and malignant labeling is carried out, and the cut image is stored in an image baseand forms a training set with the partial original images in the image base; the training set is used for training an initial thyroid tumor ultrasonic image identification model to form an advanced thyroid tumor ultrasonic image identification model; a to-be-identified thyroid tumor ultrasonic image is acquired, a tumor area is selected, and a certain edge range of the image is amplified for cutting, and the advanced thyroid tumor ultrasonic image identification model is used for identifying the benign and malignant. The method can reuse a case image, and learning, memorizing and accumulatingcharacteristics of the thyroid tumor image is achieved; with the case increasing, the generalization capability and predicting accuracy of the model are gradually improved, which plays an important role in accumulating clinical diagnosis experience.
Owner:FUNDAN UNIVERSITY SHANGHAI CANER CENTER +1

Service state online detection method and system based on components

The invention relates to a service state online detection method and system based on components, belonging to the computer field. The system comprises a component automatic generation module, a component management module, a component updating module, a component configuration module, a service state detection module, a statistics and analysis service module and a component output interface. The detection method comprises the following steps: initializing the system, starting the components, component executing, changing the configurations of the components, updating the components and logging out the system. The system of the invention adopts component architecture to provide the service state detection function automatically, efficiently and instantly; the system supports dynamic extension, and can update the components one by one without influencing the system; and the system can form a component gallery supporting various service state detections and effectively realize the reuse and accumulation of the technology.
Owner:DATCENT TECH

Method and system for splitting and combining information certificates of distributed data

The invention relates to a method and system for splitting and combining information certificates of distributed data. The information certificate of the distributed data is split as follows: a first information certificate generated by a first user and passing consensus verification is split into one or more second information certificates by a second user; the second information certificates are used for marking the relationship of rights and interests between the first user and a third user; the first user and the third user carry out right and interest data operation according to the relationship of rights and interests marked by the multiple second information certificates passing consensus verification and reaching the valid period; the information certificates of the distributed data are combined as follows: the multiple first information certificates generated by the first user and passing consensus verification are combined into the second information certificate by the second user; the second information certificate is used for marking the relationship of rights and interests between the first user and the third user; and the multiple first users and the third user carry out right and interest data operation according to the relationship of rights and interests marked by the second information certificate passing consensus verification and reaching the valid period.
Owner:BUBI BEIJING NETWORK TECH CO LTD +1

Multi-element universal model platform modeling method, electronic equipment and computer readable storage medium

The invention discloses a multi-element universal model platform modeling method. The method comprises the following steps of: establishing a data processing universal process, accessing a data universal format, and establishing a sample library and a feature library for pre-stored user information according to the data processing universal process and the data universal format; establishing a model algorithm library for processing multi-element scene businesses, and providing the model algorithm library to a preset configuration file for calling; and receiving a modeling instruction of a specific scene business, calling a model algorithm corresponding to the specific scene business from the model algorithm library through the configuration file, and carrying out modeling through the modelalgorithm corresponding to the specific scene business according to user information, according with the specific scene business, in the sample library and the feature library. According to the method, modeling can be rapidly carried out by utilizing a multi-element universal model, so that accumulation of resource parameters and model parameters is realized and periodical analysis results are output.
Owner:PING AN TECH (SHENZHEN) CO LTD

Method for increasing content of cucurbitadienol in momordica grosvenori

The invention discloses a method for increasing the content of cucurbitadienol in momordica grosvenori. Jasmonic acid methyl ester is added in the tissue culture process and / or planting process of the momordica grosvenori. The method is capable of effectively increasing the yield of the cucurbitadienol in the momordica grosvenori, has the advantages of being rapid in effect taking, low in cost, simple to operate, convenient to implement, free of toxin or residue and the like, is capable of meeting large-scale production of the cucurbitadienol in the momordica grosvenori, and has relatively good practicability and high popularization values.
Owner:GUANGXI BOTANICAL GARDEN OF MEDICINAL PLANTS

Intelligent motor system

The invention provides an intelligent motor system and belongs to the field of intelligent electrical engineering. The system includes an integrated intelligent on-line monitoring device, a driving control device, a portable device and a cloud server. The integrated intelligent on-line monitoring device and a motor are integrated into a whole so as to achieve long-term, continuous and real-time big data monitoring and accumulation and the use of almost all relevant data of factory data, historical data and real-time operation data. On this basis, motor intelligent fault diagnosis and state early warning are conducted, and operating personnel are timely informed by the portable device to achieve intelligent operation management and maintenance; the driving control device directly conducts intelligent operation control over the motor, and a manufacturer finds and analyzes product defects and effectively improve the after-sale service quality; the use of a two-dimensional code achieves tracking of all data during the whole service life time of the motor. The system has the advantages of being high in intelligence degree, simple in structure, low in cost, high in practicability, convenient to use and the like.
Owner:HARBIN UNIV OF SCI & TECH +1

Method for preparing trehalose

The invention belongs to the fields of fermentation and production of microorganisms, and in particular relates to a method for improving the yield of trehalose in a yeast strain by a dry dehydration method. A method for preparing the trehalose comprises the following steps of: fermenting the yeast strain at the temperature of between 30 and 35 DEG C, performing enlargement culture, collecting thalli, performing extrusion dehydration, and performing forced air drying on the dehydrated thalli at the temperature of between 30 and 95 DEG C until the water content of the thalli is 5 to 25 percent; performing ultramicro crushing on the dried thalli to obtain wall breaking thalli powder; and dissolving the thalli powder in water, uniformly mixing, performing ultrafiltration by using an ultrafiltration membrane, and collecting filtration liquor to obtain a solution in which the trehalose is dissolved. By the method, the water content of the yeast thalli is gradually reduced through forced air drying, the yeast thalli is stimulated to synthesize the trehalose in large quantities under a dry environment, the accumulation amount of the trehalose in the yeast thalli is improved, and the content of the trehalose in a yeast cell can be improved by 85 percent.
Owner:SHANDONG TIANLI PHARMA

Culturing method capable of promoting grease accumulation in chlorella

The invention discloses a culturing method capable of promoting grease accumulation in chlorella. The culturing method is characterized by comprising the following steps of: (1) dissolving salicylic acid or 24-epibrassinolide in ethanol to prepare a first mother solution and a second mother solution having the corresponding concentrations of 0.5-2 mg / mL; (2) culturing the chlorella until an OD (Outside Diameter) value reaches to 0.1-3.0; (3) adding the first mother solution, the second mother solution or both the first mother solution and the second mother solution into a chlorella culture solution according to a proportion; and (4) continuously illuminating the culture solution in which the first mother solution, the second mother solution or the mixture of the first mother solution and the second mother solution is added under the conditions of 25 DEG C, 40001x of the light intensity and 12 hours every day of the photoperiod and then continuously culturing for 12 hours under in a dark environment; and culturing the chlorella until the color of the chlorella is changed from green to yellow so as to obtain the chlorella with a relatively high content of the grease. The culturing method capable of promoting the grease accumulation in the chlorella has the advantages of increasing the grease accumulation speed of the chlorella efficiently and increasing the grease content of the chlorella obviously.
Owner:NINGBO UNIV

Method for constructing escherichia coli genetic engineering bacteria for producing fumaric acid

InactiveCN102618570ARealize accumulationCumulative acid production effect is idealBacteriaMicroorganism based processesEscherichia coliFumarase
The invention belongs to the field of biochemical engineering, and particularly relates to a method for constructing escherichia coli genetic engineering bacteria for producing fumaric acid. The method for constructing the escherichia coli genetic engineering bacteria mainly comprises the following steps of: inactivating or knocking fumarase serving as key enzyme for converting fumaric acid into malic acid in a tricarboxylic acid (TCA) cycle out, and knocking a key gene arcA for inhibiting the TCA cycle out. Further, key enzyme genes in paths of succinic acid, lactic acid and formic acid can also be knocked out, so the fumaric acid can be accumulated under the anaerobic condition.
Owner:NANJING UNIV OF TECH

Device and technique for producing propanoic acid by coupling of fibrous bed bioreactor with extraction separation

The invention discloses a fibrous bed reactor and the equipment and process for extracting separation and coupling production of propionic acid. The invention utilizes an immobilized fibrous bed reactor unit, a membrane separation unit, an extraction and anti-extraction unit and an elution and condensation unit to realize the efficient production of propionic acid. The device has strong feasibility, simple operation, easy realization of automation, immobilized materials, long using life of the membrane and the matching equipment, convenient maintenance and good application prospect. Through extracting separation and coupling production technology, the separation of propionic acid is realized. The invention radically eliminates the feedback inhibition of propionic acid, implements the high density fermentation of propionibacterium and greatly improves the production efficiency of propionic acid through the fermentation method. The extracted water phase is returned to the reactor for recycle after elution and condensation by an elution device, which eliminates the harm of extractant for propionibacterium from the root.
Owner:NANJING UNIV OF TECH

Engine modeling method based on behavior flow complex product function community and evolving of behavior flow complex product function community

The invention provides an engine modeling method based on a behavior flow complex product function community and evolving of the behavior flow complex product function community. The method comprises the following steps that a behavior is used for defining an initial state and a target state of a product, an appropriate behavior changing sequence achieving the state change is obtained based on the two states, and accordingly a behavior flow model in the product designing process is established; an initial mapping relation between the behavior and a function is obtained; an engine functional community and evolving model based on the behavior flow is established. An emphasis is made on carrying out the intensive study on the expression problem of the behavior flow, the behavior flow space sequence which is a brand new concept is provided in the study, the intelligent product design process based on the behavior flow is described from the angle of the space sequence evolving change, and two behavior space sequence expression modes and a conversion principle of the two expression modes are provided.
Owner:TONGJI UNIV

Fault matching and early warning method based on fault data state matrix

The invention relates to a fault matching and early warning method based on a fault data state matrix. The fault matching and early warning method comprises the following steps: S1, data normalization processing: performing data standardization on the mean value and the standard deviation of original fault data by using a Z-score standardization method; S2, calculating a similarity distance of the fault data sample vector; S3, sequencing the fault data sample vectors according to the similarity distance, and selecting the corresponding number of sample vectors to form a fault sample state matrix according to a sample vector selection rule; and S4, storing the formed fault sample state matrix into a fault database. The fault matching and early warning method has the advantages that compared with a traditional early warning system, another way is developed, and a fault data state matrix is established, and the fault matching and early warning method is more efficient; the fault database is established, so that data accumulation is realized, and a greater possibility is provided for later data utilization; and the establishment of the fault data state matrix is realized by a computer, so that personal errors are greatly reduced.
Owner:ZHEJIANG ZHENENG TECHN RES INST

GIS-based engineering project monitoring management system

The invention discloses a GIS (Geographic Information System)-based engineering project monitoring management system. The system comprises a GIS engineering project supervision platform, an engineering detection information monitoring subsystem, a construction vehicle and vessel information monitoring subsystem, an inspection tour aerial photography information monitoring subsystem and an expandable information monitoring subsystem. The GIS-based engineering project monitoring management system can meet the demands of quickly, timely and comprehensively monitoring and detecting changes of geological and hydrological environments of parts of a construction bank side and an underwater region as well as states and engineering quality of parts of construction.
Owner:CCCC FIRST HARBOR ENG +2

Similar model retrieval implementing method based on three-dimensional labeling

The invention provides a similar model retrieval implementing method based on three-dimensional labeling. By means of the method, the function of extracting three-dimensional model labeling information is achieved through three-dimensional CAD software, and a three-dimensional model labeling feature database is formed with extracted size labeling information. The size labeling information of the current three-dimensional model is extracted when similar retrieval needs to be conducted on the three-dimensional model, and the extracted size information is processed, the similarity between the size information and the model size classification and counting standard value is calculated, and the similarity of the current three-dimensional model is obtained. Then, one or more pieces of three-dimensional model data the highest in the current model similarity are retrieved and returned according to the calculated similarity, and a similar data set is formed. The similarity between the size information and the size information of each three-dimensional model in the similar data set is calculated secondarily, similarity sequencing is conducted again, and in combination with a light-weight model, the result is presented in a visual mode. By means of the method, work efficiency is remarkably improved, and design capacity is improved.
Owner:CAPITAL AEROSPACE MACHINERY +1

Installation and debugging method of Beidou generation-II navigation system electric iron tower deformation monitoring device based on machine learning

The invention belongs to the technical field of the maintenance and monitoring of an electric power device, and relates to an installation and debugging method of Beidou generation-II navigation system electric iron tower deformation monitoring device based on machine learning. The method comprises the steps: firstly performing one-dimensional linear regression analysis and least square method verification for iron tower acquisition data, carrying out signal-noise separation for debugging data by adopting a wavelet transformation method, establishing an auto-regressive and moving average (ARMA) model for the debugging data by adopting a time sequence analysis method, and finally evaluating, by experience, the prediction precision by adopting an evaluation assuming method, and analyzing and determining an optimum quantity, an optimum position and an optimum angle of a sensor.
Owner:STATE GRID CORP OF CHINA +1

Customization method and device of individual voice

An embodiment of the invention provides a customization method and device of individual voice. The customization method comprises the steps that a mobile terminal collects voice input from a microphone of the mobile terminal in a conversation process; the collected voice is processed, feature libraries belonging to personal voice are accumulated; voice output and / or match are executed by means of the feature libraries. According to the method and device, a novel voice collecting mode according to the voice synthetic technique is provided, collection of the personal voice of users is greatly facilitated by using the novel voice collecting mode, and output accuracy of voice synthesis is improved.
Owner:BEIJING OAK PACIFIC NETSCAPE TECH DEV

Detection method of leptospira

The invention discloses a detecting method for leptospira, which is based on the quantitative detection technique of nucleic acid isothermal expansion, which is characterized in that a specific primer is used, and the specific area of target gene is expanded utilizing the LAMP technological platform; under the assistance of a series of quality control and internal control detection system, the leptospira is detected in molecule level, which can realize the qualitative and quantitative detection to micro sample. Compared with the prior art, the detecting method for leptospira has the advantages of decreased non-specific expansion because of isothermal expansion, good specificity, fast detection speed, high sensitivity, no need for special equipment, low cost, easy operation, easy popularization and wide application prospect, and is suitable for experimental study, clinical detection and environment detection.
Owner:ZHEJIANG UNIV

Recombinant Bacillus subtilis for efficiently synthesizing acetylglucosamine

The invention discloses recombinant Bacillus subtilis for efficiently synthesizing acetylglucosamine and belongs to the field of genetic engineering. Recombinant Bacillus subtilis BSGN6-PxylA-glmS is used as a starting strain, glucosamine synthetic route is enhanced by overexpressing glucosamine acetylase coding gene (CaGNA1) from Candida albicans SC5314 or knocking out unnecessary area skin at the same time, and accumulated acetylglucosamine Bacillus subtilis genetically-engineered bacteria are obtained; the yield is 21% or 94% higher than that of CaGNA1 genetically-engineered bacteria from overexpressed Saccharomyces cerevisiae, and the basis is laid for the further metabolic engineering modification of Bacillus subtilis.
Owner:SHANDONG RUNDE BIOTECH CO LTD

Power generation rainwater draining pipe

The invention provides a power generation rainwater draining pipe, belongs to the technical field of draining pipes and solves the problem that water flowing in rainwater draining pipes in the prior art is unsuitable for working of a power generator. The power generation rainwater draining pipe comprises a pipe body, a water inlet and a water outlet, a floating body is arranged in the pipe body, an inner cavity of each of the water inlet and the water outlet is funnel-shaped, a conical filtering plug is arranged in the water inlet, a conical plug is arranged in the water outlet and connected with the floating body through a connecting wire, an inverted-U-shaped pipe is arranged in a manner of penetrating a wall of the pipe body, one port of the inverted-U-shaped pipe is positioned above the conical plug in the pipe body while the other port of the same is positioned outside the pipe body, and a turbine generator is arranged on the inverted-U-shaped pipe. The power generation rainwater draining pipe is simple in structure and low in cost, rainwater is drained stably by utilizing a siphon pipe principle, and working of the power generator is facilitated.
Owner:ZHEJIANG HUXIN STAINLESS STEEL MFG

Cupriavidus, cupriavidus preparation, and application of cupriavidus preparation in heavy metal contaminated soil remediation

The invention discloses cupriavidus, a cupriavidus preparation, and application of the cupriavidus preparation in heavy metal contaminated soil remediation. The cupriavidus sp. is resistant to heavy metal ions, and capable of adsorbing the heavy metal ions, increasing pH of the soil water phase micro-environment and producing struvite with a nitrogen and phosphorus slow controlled-release fertilizer function; when the cupriavidus is used for heavy metal contaminated soil remediation, in particular to cadmium contaminated soil remediation, cadmium ions in soil can be fixed through adsorption and deposition, the bioavailability of soil cadmium is reduced, the soil fertility can be improved, and the technical problems such as soil acidification are solved.
Owner:XIANGTAN UNIV

Astaxanthin synthetic gene recombinant plasmid as well as preparation method and application of astaxanthin synthetic gene recombinant plasmid

The invention discloses an astaxanthin synthetic gene recombinant expression plasmid pET-Ast with a base sequence as shown in SEQ ID NO: 1. The invention also discloses a preparation method of the recombinant expression plasmid pET-Ast, an application of the recombinant plasmid pET-Ast to the detection of astaxanthin synthetase activity of a target receptor, and an application method. After the astaxanthin synthetic gene recombinant expression plasmid pET-Ast provided by the invention is introduced to an Escherichia coli BL21 strain, the accumulation of astaxanthin in a cell body of Escherichia coli is successfully realized, so that an astaxanthin production strain can be accurately and rapidly established. The preparation method of the astaxanthin synthetic gene recombinant expression plasmid pET-Ast is simple and convenient, easy to operate and low in cost. By using the method disclosed by the invention, a gene sequence to be detected in the target receptor can be replaced with a related gene sequence in the recombinant expression plasmid pET-Ast through digestion and connection, and then, the function of a gene to be detected in the target receptor can be simply and effectively detected and verified through detecting whether the astaxanthin is accumulated in a host cell body.
Owner:HEBEI UNIVERSITY
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