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Cupriavidus, cupriavidus preparation, and application of cupriavidus preparation in heavy metal contaminated soil remediation

A technology of contaminated soil and copper greedy bacteria, which is applied in the restoration of contaminated soil, bacteria, and microbial-based methods to achieve good tolerance, achieve adsorption and accumulation, and reduce the effect of cadmium content

Active Publication Date: 2018-12-21
XIANGTAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the above-mentioned deficiencies in existing biotechnology remediation of heavy metal-contaminated soils, the first object of the present invention is to provide a method that can tolerate heavy metal ions, absorb heavy metal ions, increase the pH of the soil water phase microenvironment and produce nitrogen and phosphorus slow control Cd02 (Cupriavidus sp.Cd02) of struvite-releasing fertilizer

Method used

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  • Cupriavidus, cupriavidus preparation, and application of cupriavidus preparation in heavy metal contaminated soil remediation
  • Cupriavidus, cupriavidus preparation, and application of cupriavidus preparation in heavy metal contaminated soil remediation
  • Cupriavidus, cupriavidus preparation, and application of cupriavidus preparation in heavy metal contaminated soil remediation

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Embodiment 1

[0029] Isolation, screening and verification of cadmium tolerance of cadmium-resistant bacterial strain Cd02

[0030]The strain was isolated, screened and purified from cadmium-contaminated soil samples. The specific method is as follows: Take 1g of the above soil sample, add 100mL of sterile water, shake it in a constant temperature shaker with a rotation speed of 150rpm for 24h, let it stand for 30min, take 1mL of the supernatant and spread it on 100mg / L cadmium-containing solid LB culture In the medium, when there are bacteria growing around, pick the bacteria and streak in the solid LB medium, put them in a constant temperature incubator for 24 hours, observe the colony shape, and streak the grown single colony to the fresh LB solid Cultivate it on a plate to obtain a pure strain named Cd02. Finally, the Cd02 strain was streaked on an inclined test tube, placed in a constant temperature incubator at 30°C for 24 hours, and then stored in a refrigerator at 4°C. The formula...

Embodiment 2

[0040] Changes in pH of the culture medium and its ability to produce alkali during the culture of Cd02.

[0041] Inoculate 2% (thalline concentration 0.93~1.86g / L) Cd02 bacterial suspension in logarithmic phase of growth to nutrient solution (every liter of culture solution contains: 0.5g K 2 HPO 4 , 1g NH 4 Cl, 1g Na 2 SO 4 , 0.1g CaCl 2 2H 2 O, 2g MgSO 4 ·7H 2 (2, 2g sodium lactate), the cadmium ion concentration is respectively set to 0mg / L, 2mg / L, 4mg / L, 6mg / L, puts the vibration culture under 30 ℃ of rotating speed 150rpm, respectively in 24h, 48h, 72h, 96h, 120h, Samples were taken at 144h, and the pH of the culture solution was measured with a pH meter. Each group has 3 repetitions.

[0042] Experimental results such as Figure 5 As shown in (A), the pH of the cadmium-free culture solution increases with time, and reaches 8.6 in the later stage of cultivation; in the cadmium-containing culture solution, the pH of the culture solution also increases with the i...

Embodiment 3

[0045] Adsorption and accumulation of heavy metal cadmium ions by Copper greedy bacteria Cd02.

[0046] Inoculate 2% (0.93~1.86g / L) Cd02 bacterial suspension in logarithmic phase of growth to cadmium-containing culture solution (every liter of culture solution contains: 0.5g K 2 HPO 4 , 1g NH 4 Cl, 1g Na 2 SO 4 , 0.1g CaCl 2 2H 2 O, 2g MgSO 4 ·7H 2 (2, 2g sodium lactate), the concentration of cadmium ions is set to 2mg / L, 4mg / L, 6mg / L, 8mg / L respectively, and the rotation speed is 150rpm at 30°C for shaking culture, respectively in 24h, 48h, 72h, 96h, 120h, Take samples at 144 hours, and measure the cadmium ion content in the solution and the cadmium ion content in the bacterial cell wall and the cells with an atomic absorption spectrophotometer (flame photometry). Each group has 3 repetitions.

[0047] Experimental results such as Image 6 As shown, during the culture period, when the pH value increased from 7.5 to 8.6, due to H + The reduction of the concentration...

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Abstract

The invention discloses cupriavidus, a cupriavidus preparation, and application of the cupriavidus preparation in heavy metal contaminated soil remediation. The cupriavidus sp. is resistant to heavy metal ions, and capable of adsorbing the heavy metal ions, increasing pH of the soil water phase micro-environment and producing struvite with a nitrogen and phosphorus slow controlled-release fertilizer function; when the cupriavidus is used for heavy metal contaminated soil remediation, in particular to cadmium contaminated soil remediation, cadmium ions in soil can be fixed through adsorption and deposition, the bioavailability of soil cadmium is reduced, the soil fertility can be improved, and the technical problems such as soil acidification are solved.

Description

technical field [0001] The present invention relates to a copper greedy bacteria, and also relates to a preparation containing copper greedy bacteria and its application in cadmium-contaminated soil. The copper greedy bacteria has high-efficiency resistance to heavy metals, self-induced pH increase to improve the adsorption, accumulation and precipitation of heavy metal ions, And accompanied by the production of magnesium ammonium phosphate (commonly known as struvite) with slow and controlled release fertilizer of nitrogen and phosphorus, it is especially suitable for the remediation of cadmium-contaminated soil. The invention belongs to the technical field of microbial treatment of heavy metal polluted environment. Background technique [0002] The existing farmland heavy metal pollution is mainly cadmium (Cd) pollution, especially the acidification of farmland has further increased the activity, migration and diffusion of heavy metals in heavy metal-polluted farmland soil...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10B09C2101/00C12N1/20C12N1/205C12R2001/01
Inventor 李峰郑扬唐艺欣李程程田江刘兴旺葛飞
Owner XIANGTAN UNIV
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