The invention relates to a high-speed structure illumination
optical microscope system and a method based on a
digital micromirror device. The high-speed structure illumination
optical microscope system comprises an illumination
light source, a light splitting
prism, a
structure light generator, a lens, a spectroscope, a microobjective, an objective table, a reflector, a tubular mirror and a
charge coupled device (CCD) camera, wherein the light splitting
prism is arranged on a light path of the illumination
light source, the
structure light generator is arranged on a reflection light path of the light splitting
prism, the lens is arranged on a transmission light path of the light splitting prism, the spectroscope is arranged on a lens light path, the microobjective and the objective table are arranged on a light path above the tubular mirror, the reflector and the tubular mirror are arranged on a light path under the spectroscope, and the
CCD camera is arranged behind the tubular mirror. The
system and the method aim at the technical problems of low
optical energy utilization rate and
low speed of the existing structure illumination
microscope and have the advantages that the image refreshing speed is high (32KHz), and the
optical energy utilization rate is high (higher than 90 percent). The system and the method are more applicable to the real-time three-dimension
image study and high-speed dynamic process observation of living biological cells.