The invention relates to a method and special mould for preparing a tissue and organ precursor with multi-
branch channels, wherein the special mould comprises a base mould, at least
one stage of inner mould and at least
one stage of outer mould. The method provided by the invention comprises the steps that: the inner mould and the outer mould are arranged on the base mould in sequence according to the stages, when each stages of mould is mounted, a
matrix solution containing cells is poured in the outer mould cavity, after the crosslinking of the
matrix solution containing cells, the outer mould and the inner mould are removed, so that a layer of
cell matrix layer with channels is formed; then the above steps are repeated, so that multiple
layers of the
cell matrix layer with channels are prepared in sequence, the interlayer channels are interconnected in
branch-shaped, the multilayer
cell matrix layer is penetrated and adhered through the
synthetic polymer solution to form the tissue and organ precursor with multi-
branch channels, and the shaped multi-branch channels can form the structures, such as
blood vessel, nervus,
bile duct, trachea and the like. The method provided by the invention overcome a defect that it is difficult to construct the complex branch channel in prior technology, and has advantages including that: the range of shaped tissue and organ is wide, the mounting of matrix materials containing
high density of cells is easy to be realize.