The invention relates to a novel cardiac valve implantation instrument with an anchoring device. The novel cardiac valve implantation instrument comprises a cardiac valve prosthesis, at least two sets of anchoring needles and at least two sets of anchoring needle releasing devices detachably connected with the cardiac valve prosthesis. The far ends of the anchoring needles are in preset shapes, the anchoring needle releasing devices comprise conveying pipes, push rods and hollow guide rails, the guide rails are arranged in the conveying pipes in advance and can move towards the far ends of the conveying pipes, rigid sections are arranged at the far ends of the guide rails, the anchoring needles are arranged in the guide rails in advance in an extension state, the push rods are arranged in the conveying pipes and located at the near ends of the anchoring needles, the far ends of the anchoring needle releasing devices are in preset shapes, the far ends of the conveying pipes can be overall bent or partially bent, the guide rails and the anchoring needles can move towards the far ends of the conveying pipes under the action of external force, and the cardiac valve prosthesis is fixedly arranged between the autologous tissue and the anti-falling ends of the anchoring needles. The novel cardiac valve implantation instrument can achieve accurate locating and firm anchoring, and the treatment success rate is improved.