The invention discloses a multi-view pixel directional backlight module and a naked-eye 3D display device. The multi-view pixel directional backlight module comprises at least two rectangular light guide plates, wherein all the rectangular light guide plates are tightly stacked together, multiple pixels which are formed through nanometer diffraction gratings and point to different directions are formed on the light emergence surface of each rectangular light guide plate, at least one side edge of each rectangular light guide plate is provided with a light source set, and emergent light is formed by light emitted by the light source sets on pixels of multiple pixel arrays on the light emergency surfaces of the light guide plates after the light emitted by the light source sets enters the corresponding light guide plates. According to the multi-view pixel directional backlight module and the naked-eye 3D display device, due to the fact that the multiple light guide plates are stacked, the problem that mutual interference of three light sources can not be avoided on one light guide plate is solved, the pixel directional rectangular light guide plates are obtained, a feasible scheme is provided for industrial application of the multi-view pixel directional light guide plates in the naked-eye 3D display technique, and the problems which can not be solved in the prior art are solved.