The invention discloses a
reagent for extracting and purifying
DNA, comprising the following substances: preprocessing
lysis solution with pH of 7.4-8.5: water serves as
solvent, and solute comprises the substances with the following final concentration: 10-100mM of buffer salt, 0.5%-5% of surface
active agent, 1-100mM of chelating agent, 50-150mM of soluble salt, and 0.2-4mg / ml of
protease K;
pyrolysis combined liquid with pH of 6.4-7.4: water serves as
solvent, and solute comprises the substances with the following final concentration: 20-150mM of buffer salt, 3-8 of MChaotropic salt, 1-10% of surface
active agent, 0.5-4% of amphoteric
ion detergent, 10-100mM of chelating agent and 15-30% of
alcohol; cleaning solution I: water serves as
solvent, and solute comprises the substances with the following final concentration: 4-6 of MChaotropic salt, 25-50% of
alcohol and 10-100mM of chelating agent; cleaning solution II is 75% of
ethanol water solution; spent regenerant is TE (10mM
Tris-HCl, 1mMEDTA, and pH is 8.0);
magnetic bead suspension is nanoscale
silicone coated magnetic
microsphere (50-100mg / ml).The
DNA extracting efficiency is as high as 93.67% by adopting the
reagent.