Prepn process of selenium-rich edible fungus with activated selenium ore as selenium source
A technology for selenium-enriched edible fungi and activation of selenium ore is applied in the field of biotransformation of selenium resources, which can solve problems such as poor physiological activity, achieve good economic benefits, and realize the effects of transformation and enrichment.
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Embodiment 1
[0023] 1) Activation of selenium ore:
[0024] Crush and sieve the selenium ore, the fineness of the ore powder is 40 mesh; weigh 500g of the selenium ore powder, add 1500mL of ammonia water with a concentration of 0.60mol / L, stir at room temperature for 24 hours, then heat the solution to boil and boil to one third of the volume, then naturally cooled to room temperature and added 1000mL of ammonia water with a concentration of 0.60mol / L, then boiled to one third of the volume and cooled to room temperature, and added 1000mL for the second time with a concentration of 0.60mol / L Ammonia water and continue to heat to boil and cook to 1 / 3 volume stand-by;
[0025] Add 1000mL of sodium hydrogen phosphate with a concentration of 0.2mol / L - sodium dihydrogen phosphate buffer solution (pH = 8.5), heat to boil and cook until the total volume of the mixture is 1000mL, stop heating, cool to room temperature and centrifuge in a centrifuge (3000r / min) 15min, the supernatant obtained is ...
Embodiment 2
[0033] 1) Activation of selenium ore:
[0034] Crush and sieve the selenium ore, the fineness of the ore powder is 80 mesh; weigh 500g of the selenium ore powder, add 1500mL of ammonia water with a concentration of 0.60mol / L, stir at room temperature for 36 hours, heat the solution to boiling and cook to one third of the volume, then naturally cooled to room temperature and added 1000mL of ammonia water with a concentration of 0.60mol / L, then boiled to one third of the volume and cooled to room temperature, and added 1000mL for the second time with a concentration of 0.60mol / L Ammonia water and continue to heat to boil and cook to 1 / 3 volume stand-by;
[0035] Add 1000mL of sodium hydrogen phosphate with a concentration of 0.2mol / L - sodium dihydrogen phosphate buffer solution (pH = 8.5), heat to boil and cook until the total volume of the mixture is 1000mL, stop heating, cool to room temperature and centrifuge in a centrifuge (3000r / min) 15min, the supernatant obtained is th...
Embodiment 3
[0043] Crush and sieve the selenium ore, the fineness of the ore powder is 180 mesh; weigh 500g of the selenium ore powder, add 1500mL of ammonia water with a concentration of 0.60mol / L, stir at room temperature for 48 hours, then heat the solution to boil and boil to one third of the volume, then naturally cooled to room temperature and added 1000mL of ammonia water with a concentration of 0.60mol / L, then boiled to one third of the volume and cooled to room temperature, and added 1000mL for the second time with a concentration of 0.60mol / L Ammonia water and continue to heat to boil and cook to 1 / 3 volume stand-by;
[0044] Add 1000mL of sodium hydrogen phosphate with a concentration of 0.2mol / L - sodium dihydrogen phosphate buffer solution (pH = 8.5), heat to boil and cook until the total volume of the mixture is 1000mL, stop heating, cool to room temperature and centrifuge in a centrifuge (3000r / min) 15min, the supernatant obtained is the activated selenite aqueous solution ...
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