1494 results about "Fungal mycelium" patented technology

The present invention utilizes the pre-sporulation (preconidial) mycelial stage of entomopathogenic fungi as insect attractants and / or pathogens. The fungus can be cultivated on grain, wood, agricultural wastes or other cellulosic material, attracting the insect and optionally introducing insect-specific pathogenic fungi. More than one fungus and substrate can be used in combination. The matrix of preconidial fungi can optionally be dried, freeze-dried, cooled and / or pelletized and packaged and reactivated for use as an effective insect attractant and / or biopesticide. Attractant extracts of the preconidial entomopathogenic mycelium are disclosed.

The invention discloses a culture medium used for cultivating bisporic mushrooms as well as the cultivating method thereof. Per 100 square meters of culture medium contain the following cultural materials: cotton seed hull 1800 to 3200 catties, waste cotton 0 to 1200 catties, corncobs 0 to 1200 catties, cottonseed meal 0 to 200 catties, cattle manure 2 to 4 cubic meters, chicken manure 0 to 1.2 cubic meters, urea 0 to 60 catties, quicklime 80 to 120 catties, plaster powder 0 to 120 catties, calcium superphosphate 0 to 120 catties, enzyme microorganisms 2 to 4 catties, and water of proper amount. The method includes the following steps: firstly, the cultural materials are mixed; secondly, the mixed cultural materials are composted and fermented; thirdly, the cultural materials are processed in a shed; fourthly, sowing is carried out; fifthly, the culture medium is distributed; sixthly, the soil is covered; seventhly, the mushrooms are obtained. The culture medium has low cost, the conglutination of the cultural materials is not easy to happen, and the cultural materials have easy pile-turning, thereby causing the composting and the fermentation to be laborsaving and timesaving; the enzyme microorganisms can not only speed up the fermentation of the cultural materials and improve the fermentation quality, but also facilitate the absorption and utilization of the culture medium by the mycelium of the bisporic mushrooms.

The invention discloses a liquid submerged fermentation culture method for hericium erinaceus, and belongs to the field of culture methods for edible fungi. The culture method comprises the following steps of: activating hericium erinaceus strains on a slant culture medium, then performing primary strain culture on a liquid seed culture medium, performing secondary strain culture on a liquid fermentation culture medium, and finally performing amplified culture on the liquid fermentation culture medium to obtain hericium erinaceus mycelium. The invention also discloses hedgehog hydnum 1 in the hericium erinaceus strains, a seed culture medium and a fermentation culture medium for hericium erinaceus culture. The biological yield of the hericium erinaceus mycelium obtained by the culture method is high and can reach 12g / L which is over two times as much as the fermentation production level of the current factory; the hedgehog hydnum 1 in the hericium erinaceus strains has high growth speed which can reach 5.8 millimeters every day; and moreover, the liquid seed culture medium and the fermentation culture medium have good culture effect so that the hericium erinaceus mycelium has quick growth and high yield.

The invention is directed to recombinant antibodies which bind to Sclerotinia sclerotiorum antigens and comprise a single chain variable fragment (scFv). The antigen may be selected from SSPG1d or a portion thereof, aspartyl protease or a portion thereof, or whole Sclerotinia sclerotiorum mycelium. The invention also provides an antibody linked to an anti-fungal polypeptide. The invention extends to nucleic acid sequences encoding the antibodies, and expression vectors comprising the nucleic acid sequences. The invention is also directed to transgenic plants, seeds, tissues or cells transformed with the expression vectors. Methods for producing a transgenic plant that is resistant to Sclerotinia sclerotiorum, and for detecting Sclerotinia sclerotiorum in a biological sample utilizing an antibody which binds to Sclerotinia sclerotiorum antigen, and immunoassay kit for same are also provided.

The method of growing a fungal fruiting body requires exposing a mycelium of a desired organism type to environmental conditions sufficient to induce fruiting of fungal primordium in the organism type followed by enclosing the fungal primordium within a mold of a designated shape representing a near net shape volume of a desired final product. The fungal primordium is allowed to grow and fill the mold to form a mass of fungal tissue equivalent in shape to the designated shape of the mold after which the mass of fungal tissue is removed from the mold and dried.

A method of preparing a mycelium component, including growing live mycelium from a fungal inoculum and a liquid aggregate. The live mycelium is cured to terminate further mycelium growth and form a mycelium structure. Moisture is applied to the mycelium structure such that the mycelium structure is nearly fully saturated. The mycelium structure is dried to form a hardened mycelium structure.

The invention discloses a natural planting method of a mushroom, solving the problems of low quality and easiness of manual pollution because the traditional mushroom planting technology is limited in the room. The natural planting method comprises the following steps of: 1, culturing a strain: separating a field-planted sporocarp to obtain a patent seed, culturing the patent seed according to the conventional expanding propagation method, continuously culturing a breeder seed to obtain a planting strain; 2, culturing a mycelium; 3, selecting a natural environment for planting: selecting an original forest, a secondary forest and an artificial forest with the ground temperature of 4-15 DEG C, the air humidity of 42-88 percent, the soil pH value of 6-7 and the forest land and grassland canopy density of 0.3-0.7; and 4, managing fruiting. The mushroom is cultured and planted by using the natural environment, and the mushroom sporocarp grows and develops under a natural and wild condition to ensure that the growth environment of the mushroom is more excellent and the product quality is better.

The process provides a biofilm including and not limited to cellulose produced by bacteria that can be used as a bio-resin and as a surface application for myceliated and non-myceliated biomaterials.In one embodiment, the process comprises the steps of obtaining an agricultural substrate; and cohabitating a selected bacteria with a selected fungus in the agricultural substrate for a period of time to allow the bacteria to grow alongside the fungus and to excrete a biofilm from the bacteria into the substrate to provide bio-resin like strengthening compounds to the agricultural substrate.

The invention discloses a cultivation method of tuckahoe, which comprises the steps of: (1) firstly, the epidermis of the tuckahoe is cut; the tuckahoe is superficially sterilized by 70 to 75% of ethanol and washed with sterile water; the water content of the tuckahoe is blotted up by disinfection cloth or a filter paper; in an aseptic inoculation box, an alcohol burner is lightened, the processed tuckahoe is sliced off by an abacterial knife near fire and the cross section is scraped into be cambered and successively scraped for twice; on the second scraping position, blocks like wheat size are connected on a pure strain culture medium, placed in a greenhouse, and cultured in the temperature of 25 to 30 DEG C till mycelial overgrows; the blocks which are white, are not infected by mixed bacterium, are villous, dense and craggy, vigorously and uniformly grow, secretes white milk pearl mycelium are pure strains; (2) a primary strain cultivation is carried out; (3) a second class strain cultivation is carried out; (4) a third class strain cultivation is carried out. The method is characterized by short production period of mycelium, excellent and stable tuckahoe quality and high yield.

The invention relates to a zymocyte mixture containing lucid ganoderma. A mycoplasm mixture containing hypha and culture matrix components is obtained by fermenting the lucid ganoderma in a solid fermentation mode in a nutritional culture matrix which at least comprises a carbon source and a nitrogen source needed by the growth of fungi, wherein medical raw materials salvia miltiorrhiza are mixed in the culture matrix. A mycoplasm fermentation final product having both the effects of the salvia miltiorrhiza and the ganoderma fungi is obtained after bidirectional solid fermentation including influence of medical raw materials on the growth and metabolism process of ganoderma mycelium and transformation of a ganoderma fungal enzyme system on the medical materials compositions, and the invention can be applied to preparing a medicament and / or a cosmetic with the effects of promoting blood circulation by removing blood stasis and antioxidation.