97 results about "Entomopathogenic fungi" patented technology

A probe, a set of probes, and a probe carrier on which the probe or the set of probes is immobilized, are provided for classification of fungus species. The probe or the set of probes is capable of collectively detecting fungus of the same species and distinguishingly detecting those fungus from fungus of other species. The probe is an oligonucleotide probe for detecting a pathogenic fungus DNA and includes at least one of base sequences of SEQ ID NOS. 1 to 4 and mutated sequences thereof.

The present invention utilizes fungal spore mass or hyphal fragments in burlap bags or sacks filled with biodegradable materials. The fungi may include saprophytic fungi, including gourmet and medicinal mushrooms, mycorrhizal fungi, entomopathogenic fungi, parasitic fungi and fungi imperfecti. The fungi function as keystone species, delivering benefits to both the microsphere and biosphere. Such fungal delivery systems are useful for purposes including ecological rehabilitation and restoration, preservation and improvement of habitats, bioremediation of toxic wastes and polluted sites, filtration of agricultural, mine and urban runoff, improvement of agricultural yields and control of biological organisms.

The present invention utilizes the pre-sporulation (preconidial) mycelial stage of entomopathogenic fungi as insect attractants and / or pathogens. The fungus can be cultivated on grain, wood, agricultural wastes or other cellulosic material, attracting the insect and optionally introducing insect-specific pathogenic fungi. More than one fungus and substrate can be used in combination. The matrix of preconidial fungi can optionally be dried, freeze-dried, cooled and / or pelletized and packaged and reactivated for use as an effective insect attractant and / or biopesticide. Attractant extracts of the preconidial entomopathogenic mycelium are disclosed.

The present invention utilizes extracts of the pre-sporulation (preconidial) mycelial stage of entomopathogenic fungi as insect and arthropod attractants and / or pathogens and can be employed to limit the zoonotic and plant diseases they transmit. The fungus can be cultivated on grain, wood, agricultural wastes or other cellulosic material and extracts can be made thereof. More than one fungus and substrate can be used in combination with one or more antimicrobial, antiprotozoal, antiviral, or genetically modified agents that result in reduced spread of contagions and lessens the damage they inflict on animals and plants.

Relating to technical field of microorganisms, the invention discloses a beauveria bassiana XNBb-04 strain and a culture method thereof. The beauveria bassiana XNBb-04 strain is preserved in China Center for Type Culture Collection on March 6, 2014, and the preservation number is CCTCC M 2014075. The beauveria bassiana XNBb-04 strain provided by the invention belongs to Deuteromycota, Moniliales, Moniliaceae, Beauveria. The beauveria bassiana XNBb-04 strain is a native entomopathogenic fungus of China, can be further used for preparation of living biopesticides, has good pathogenic effect on nodulated alfalfa weevils, can effectively kill nodulated alfalfa weevils, has brand new action mechanism different from existing chemical insecticides, and has no environmental pollution or residue. The beauveria bassiana XNBb-04 strain provided by the invention can be applied to various ecological environments in terms of pest control, and is conducive to environmental protection.

Microsclerotia of entomopathogenic fungi, including Metarhizium species, Beauveria species, and Lecanicillium species, may be produced. These microsclerotia are effective for the control of a wide variety of insect pests, particularly soil-dwelling insect pests.

The present invention relates to a method for manufacturing a fungal granule for controlling insects. The invention provides a method for preparing an entomopathogenic fungi sodium alginate granule and a granular formulation prepared through the method. Compared with the prior art, the invention not only preserves the normal advantages of long-time shape keeping of algae colloidal particle and growth recovering of fungus in the particles, but also has other innovations and improvements on other aspects. The algae colloidal particles containing fungus are used for being sprinkled to the farmland or woodland for controlling insects.

The invention discloses application of evodiamine and rutaecarpine in preparation of a medicine for enhancing the activation of NLRP3 inflammasomes. The evodiamine and the rutaecarpine can generate active Caspase-1 by enhancing activiation of macrophage inflammasomes so as to release manure inflammatory mediators such as IL-1beta and promote pyroptosis of sensitized macrophages. The activity of the evodiamine and the rutaecarpine can be used for enhancing the inflammatory response intensity of a human body and treating related diseases of insufficient activation of inflammasome including pulmonary tuberculosis and oral tuberculosis caused by intracellular infection of mycobacterium tuberculosis, diseases caused by listeria monocytogenes, related diseases caused by other intracellular infection bacteria, related diseases caused by pathogenic fungal infection, furunculosis, dental-root periapical diseases, chronic gastritis, chronic enteritis, dysentery and diarrhea and the like.

The invention provides an entomopathogenic fungi transgenic strain which contains a homologous or heterologous acidic trehalase constitutive exogenous gene. The preparation method of the entomopathogenic fungi transgenic strain comprises the following steps: firstly building an acidic trehalase full-length cDNA gene constitutive expression vector; establishing a submerged conidia transformation system; and finally selectively screening to obtain the entomopathogenic fungi transgenic strain. The invention also provides application of Entomopathogenic fungi acidic trehalase transgenic strain in preparing fungal pesticide. The entomopathogenic fungi transgenic strain has high efficiency of expressing trehalase; and the preparation method has the advantages of easy operation, good repeatability and good reproducibility. The entomopathogenic fungi transgenic strain can be applied to preparing the fungal pesticide. When the fungal pesticide acts on insects, through the overexpression of the acidic trehalase gene via the transgenic strain, the activity of acidic trehalase in an insect host is improved, utilization of the trehalase is accelerated and growth of entomopathogenic fungi in the insect host is promoted, thus effectively improving the insecticidal activity of the fungal pesticide such as metarhizium anisopliae, beauveria bassiana and the like.

The invention relates to antimicrobial peptide and application thereof, and aims to provide a defensin gene of antimicrobial peptide of bemisia tabaci (Gennadius), the antimicrobial peptide encoded by the defensin gene and a preparation method for the defensin gene. A nucleotide sequence of the gene of the antimicrobial peptide is shown as SEQ ID NO.1; and an amino acid of the antimicrobial peptide is shown as SEQ ID NO.2. A protease Xa factor is introduced into an expression vector, so that recombinant fusion protein can be effectively digested, and defensin polypeptide is obtained through rapid separation in an ultrafiltration mode according to the magnitude of protein molecules; the whole process is quick and simple; and the obtained antimicrobial peptide has antifungal activity, and can remarkably inhibit the growth of entomopathogenic fungi such as Beauveria bassiana, Metarhizium anisopliae, Botrytis cinerea and the like.

Disclosed herein are pest controlling compositions comprising entomopathogenic fungi which are horizontally transmissible across pest populations and control target pests at various life stages. Further disclosed are methods of using such compositions for controlling pests, including, bed bugs and other invasive parasitic pests.

The present invention provides insecticidal lipids and lipid fractions from fungi of the phylum Ascomycota, together with compositions comprising such lipids and lipid fractions and methods of preparing same. Methods for the biological control of insects, such as phytopathogenic insects, using the lipids and lipid fractions or compositions comprising said lipids optionally together with one or more insecticidal or entomopathogenic agents including entomopathogenic fungi, are also provided.