The invention discloses a preparation method of a
metarhizium anisopliae conidia microcapsule
emulsion. The preparation method comprises the following steps of disinfecting, specifically, disinfectinga culture container firstly, spraying
disinfectant fluid, putting in a high-temperature box for high-temperature disinfection, cleaning by using an ultrasonic cleaning box, and
drying a container forlater use after cleaning and disinfection; preliminary culture, specifically, selecting the disinfected container according to the volume, placing the
metarhizium anisopliae into the container afterselecting the volume, adding
hypha diaphragms into the container, carrying out
fission so as to generate
spore buds in the container, and carrying out culture for 20 hours; and carrying out medium-step culture, specifically, enabling the
metarhizium anisopliae conidia to begin to germinate, and transferring
protoplast to a
bud tube growth poin. The
fungus conidium production speed is increased, the
fungus culture time and the
fungus use space are reduced, the safety and reliability during preparation are improved through multiple reasonable disinfection in the
liquid culture stage, the phenomena of
atrophy, death and the like of the
metarhizium anisopliae are avoided, the reliability is high, and wide popularization can be achieved.