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31 results about "Blastospore" patented technology

A blastospore is an asexual fungal spore produced by budding. Produced by fungi within the phylum Glomeromycota and others. It is also known as a blastoconidium (plural = blastoconidia). An example of a fungus that forms blastospores is Candida albicans.

Compound insecticide of bacillus thuringiensis, verticillium lecanii and aconite alkaloid and preparation method thereof

The invention discloses a compound insecticide of bacillus thuringiensis, verticillium lecanii and aconite alkaloid and a preparation method thereof. The compound biotic insecticide is mainly mixed through the fermentation liquor of bacillus thuringiensis and the meristem of the verticillium lecanii or blastospore and the aconite alkaloid with proper auxiliary agent according to certain proportion, wherein the mass percentage of the main medicine and the auxiliary agent respectively accounts for 50 percent to 90 percent and 10 percent to 50 percent. The compound insecticide has the advantagesthat the compound insecticide is broad-spectrum, has control effect to various crop pests, and particularly has obvious effect to homoptera pets, lepidoptera pets and coleopteran pets; the desinsection speed is high; the lasting period is long; pets are difficult to develop drug resistance; the compound insecticide is safe to people and animals, environment and natural enemies; the manufacturing process is simple, the material sources are wide, and the cost is low; and the preparations are multiple, and the application field is wide.
Owner:GANSU ACAD OF SCI INST OF BIOLOGY

Actinomyces Israeli production technology, Actinomyces Israeli preparation and Actinomyces Israeli production apparatus

The invention discloses an Actinomyces Israeli production technology, an Actinomyces Israeli preparation and an Actinomyces Israeli production apparatus. The production technology adopts a liquid-solid biphasic cultivation method to realize the mass production of Actinomyces Israeli, liquid fermentation is carried out to obtain a large amount of mycelia and blastospores and a small amount of conidiospores, and the above obtained substances are transferred to a solid cultivation medium to generate a large amount of aerial conidiospores. An Actinomyces Israeli pill or pulvis prepared through the technology is convenient to store, and can be dissolved in a 0.1M sodium citrate solution or is diluted by adding water when the pill or pulvis is used. The production apparatus comprises a disc body, the base plate of the disc body is punched with a plurality of holes, and at least one metal screen covering all the holes is fixed on the base plate. The apparatus guarantees the oxygen and humidity required by the solid cultivation of the Actinomyces Israeli, effectively prevents the living contaminants, and substantially improves the sporulation quantity of the Actinomyces Israeli.
Owner:INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY

Liquid fermentation culture medium for cordyceps sinensis

The invention relates to a liquid fermentation culture medium for cordyceps sinensis. In additions to a carbon source, a nitrogen source, metal salts and the like in a common culture medium, the culture medium is further added with phosphorylcholine, phosphorylethanolamine, cholesterol, fatty acid and the like as a growth promoter, so that the cordyceps sinensis can be effectively promoted to form blastospore or hyphal bodies. Bacterial suspension obtained by culture medium fermentation is rich in blastospore or hyphal body growth point, and can be continuously fermented or used as a seed solution for subsequent liquid or solid fermentation, and therefore, the liquid fermentation culture medium is beneficial for improving fermentation production efficiency of the cordyceps sinensis.
Owner:SUN YAT SEN UNIV

Strain of entomopathogenic fungus isaria fumosorosea ccm 8367 (ccefo.011.pfr) and the method for controlling insect and mite pests

Strain of entomopathogenic fungus Isaria fumosorosea CCM 8367 (CCEFO.011.PFR) deposited in collection CCM (Czech Collection of Microorganisms) in Brno, applicable for biological control of substrates infested by insect and mite pests. Infested woods, plants, etc. are treated with blastospores or conidiospores of this strain.
Owner:BIOLOGICKE CENT AV CR V V I

Improved Grocott silver hexosamine staining method and applications thereof

ActiveCN104677713AOvercome the defects that are easy to cause peeling offSolve the defect of easy peeling offPreparing sample for investigationStainingHexosamines
The invention discloses an improved Grocott silver hexosamine staining method and applications thereof. The staining method comprises the steps of preparing paraffin-embedded sections, staining, carrying out dehydrated hyalinizing, and carrying out section sealing, wherein in the step of preparing paraffin-embedded sections, a sliced paraffin section is adhered to a glass slide subjected to APES treatment. The method overcomes the defect that section shedding easily occurs in the process of staining, especially for frankliniella occidentalis with a small somatotype, the non-section-shedding rate is over 70%. In addition, the internal tissues of polypides in sections obtained according to the staining method disclosed by the invention are red, blastospores in polypides are black, therefore, the polypides are clearly visible, and the staining effect is excellent.
Owner:QINGDAO AGRI UNIV

Isaria cateinannulata as well as product, preparation method and use method thereof

InactiveCN104928185AEasy to getIncrease virulenceBiocideFungiEuseius nicholsiSide effect
The invention provides isaria cateinannulata as well as a product, a preparation method and a use method thereof. Blastospore suspension liquid of isaria cateinannulata 08XS-1 bacterial strain is adopted as effective ingredients to prepare biopesticide; the biopesticide can kill insects without adopting an oral administration manner or contacting the insects, as well as can effectively prevent and treat tetranychus urticae koch on vegetables in fields; the biopesticide has incomparable advantages than other biopesticide; the blastospore suspension liquid is taken as bioactive substances, so that the isaria cateinannulata is more available and high in toxicity; in addition, the isaria cateinannulata has no side effects on euseius nicholsi, so that the isaria cateinannulata has low toxicity on natural enemies; the isaria cateinannulata can be used for further development and production of biopesticide along with brighter application and popularization prospects; the method provided by the invention is simple, easy to implement, low in cost and good in using effect.
Owner:GUIZHOU UNIV

Control of infectious microorganisms by modulation of chorionic gonadotropin-related protein activity

A method of controlling, in a warm-blooded animal, an infectious microorganism expressing a chorionic gonadotropin-like growth-regulating protein comprises modulating the activity of the protein by administering to the warm-blooded animal an effective amount of an antibody capable of inhibiting the activity of the protein, the chorionic gonadotropin-like growth-regulating protein itself such that the animal develops a neutralizing immune response, or peptides that block the receptors of the chorionic gonadotropin-like growth-regulating protein in the infectious microorganism. Candida albicans is one such microorganism that can be controlled by delivering an antibody against the growth-regulating protein, CaCGLP. Mycobacteria can also be controlled by this method. A method of inhibiting transition of Candida albicans blastospores in vitro by contacting the blastospores with an antibody against CaCGLP is also disclosed. A method of inhibiting growth of mycobacteria in vitro comprises contacting the mycobacteria with an antibody against an endogenous chorionic gonadotropin-like growth-regulating protein. In vitro growth of microorganisms can also be stimulated by contacting the microorganism with a chorionic gonadotropin-like growth-regulating protein.
Owner:UNIV OF UTAH RES FOUND

Medical health applications of sea buckthron oil for mucosal repair and nutrition

The invention belongs to the technical field of medicine health care, and in particular discloses applications of sea buckthron oil. The sea buckthron oil is used for preparing the medicines, foods or health products used for mucosal repair and nutrition, and the mucosa is skin mucosa, gastric mucosa or vaginal epithelial mucosa; the sea buckthron oil has the inhibiting effect for the skin mucosa inflammation; the sea buckthron oil has the effects of repairing the wound of the skin mucosa and promoting the growth of skin factors; the sea buckthron oil has the protection effect for the gastric mucosal damage; the sea buckthron oil can inhibit the formation of candida albicans hyphae and the adhesion of blastospores for epithelial cells of the vagina, and recover the state and vitality of the epithelial cells of the vagina.
Owner:陕西海天制药有限公司

Promoted pathogen detection method and equipment based on improved cyclic steering filtering algorithm, and storage medium

The invention relates to an improved pathogen detection method and device based on an improved cyclic steering filtering algorithm and a storage medium. The method comprises the following steps: S1, constructing a pathogen training set for detecting spores and bud spores; s2, training an artificial intelligence target detection model for detecting pathogens of spores and blastospores based on the pathogen training set of spores and blastospores constructed in S1; s3, carrying out filtering operation on the original image based on an improved cyclic steering filtering algorithm; and S4, detecting the pathogen in the input image: carrying out filtering operation on the input image according to the step S3, and then inputting the filtered image into the artificial intelligence target detection model of the pathogen trained in the step S2 for pathogen detection to obtain the position information of the pathogen. According to the invention, the algorithm based on improved cyclic guide filtering is adopted, and the processed image is input into the deep learning target detection model based on the convolutional neural network, so that the detection rate of pathogens is effectively improved.
Owner:山东仕达思生物产业有限公司 +1

Detection and identification method for automatically refining and labeling categories of spores

The invention relates to a detection and identification method for automatically refining and labeling categories of spores. The method comprises the following steps: acquiring an original image and an expert labeling document; formulating a fine classification rule of the bud spores; designing a rapid and automatic classification method based on an adaptive mask according to a fine classification rule formulated by the bud spores; intercepting all marked areas of interest of the bud spores and storing the areas of interest of the bud spores; automatically classifying the intercepted and stored areas of interest of the germinated spores according to a formulated fine classification rule and an automatic classification method; constructing a training set with a small intra-class gap and a large inter-class gap; training a fine classification AI target detection model for detecting bud spores; and summarizing the bud spores of the fine classification category detected by using the AI target detection model as a final detection result. According to the method, the detection rate of the bud spores is effectively increased, and meanwhile, the false detection rate of the bud spores is reduced; in addition, by adopting a self-adaptive mask rapid automatic classification method, the time and the labor cost of manual re-labeling are greatly reduced.
Owner:山东仕达思生物产业有限公司 +1

Spore and spore identification method and system based on residual deep network, and medium

The invention provides a spore identification method and system based on a residual error deep network and a medium, and the method comprises the steps: 1, segmenting a collected original picture through employing a morphological method, screening a target picture needing to be identified, forming a sample set, and marking the sample set; 2, performing data amplification on the labeled sample set to form a final training data set; 3, constructing a deep residual network on the caffe framework, setting hyper-parameters, and training by using the data set to obtain a deep residual network training model; and 4, performing morphological segmentation on an original picture collected in real time, performing identification scoring by using the deep residual network training model with the highest comprehensive identification rate after the test, and taking a result greater than a confidence threshold as a final identification result and outputting the final identification result. According to the method, the spores and the bud spores are identified by adopting the residual deep network structure, so that the problems that morphological identification needs a large number of parameters, the efficiency is low and the identification rate is low are solved.
Owner:JIANGSU BIOPERFECTUS TECH CO LTD

A Chinese Mortierella hirsutella sinensis transformed bacterial strain expressing green fluorescent protein and its preparation method

ActiveCN107418965BSimple materialConvenient for molecular biology researchFungiMicroorganism based processesBacterial strainFunctional genes
The invention discloses a method for preparing a Hirsutella sinensis transforming strain expressing green fluorescent protein. The method comprises: (1) preparing a Hirsutella sinensis blastospore suspension; (2) preparing Agrobacterium tumefaciens containing recombinant plasmid pRF-AETH; (3) preparing an Agrobacterium tumefaciens liquid; (4) mixing the Hirsutella sinensis blastospore suspension and the Agrobacterium tumefaciens liquid, and co-culturing; and (5) carrying out selective culture, identifying, and obtaining the Hirsutella sinensis capable of stably expressing green fluorescent protein. The invention further provides a Hirsutella sinensis transforming strain. According to the present invention, with the method, the Hirsutella sinensis transforming strain capable of stably expressing egfp gene can be obtained, can provide the convenience for the molecular biology research of the Hirsutella sinensis functional gene, can monitor the growth and reproduction and the development and differentiation of the Hirsutella sinensis in host insects, can provide the direct evidence for the clarification of the interaction between the Hirsutella sinensis and the host, and has excellent application prospects.
Owner:成都图径生物科技有限公司

A kind of production process, preparation and production device of nematocide fungus

The invention discloses an Actinomyces Israeli production technology, an Actinomyces Israeli preparation and an Actinomyces Israeli production apparatus. The production technology adopts a liquid-solid biphasic cultivation method to realize the mass production of Actinomyces Israeli, liquid fermentation is carried out to obtain a large amount of mycelia and blastospores and a small amount of conidiospores, and the above obtained substances are transferred to a solid cultivation medium to generate a large amount of aerial conidiospores. An Actinomyces Israeli pill or pulvis prepared through the technology is convenient to store, and can be dissolved in a 0.1M sodium citrate solution or is diluted by adding water when the pill or pulvis is used. The production apparatus comprises a disc body, the base plate of the disc body is punched with a plurality of holes, and at least one metal screen covering all the holes is fixed on the base plate. The apparatus guarantees the oxygen and humidity required by the solid cultivation of the Actinomyces Israeli, effectively prevents the living contaminants, and substantially improves the sporulation quantity of the Actinomyces Israeli.
Owner:INST OF FOREST ECOLOGY ENVIRONMENT & PROTECTION CHINESE ACAD OF FORESTRY

An Improved Grocott Hexamine Silver Staining Method and Its Application

ActiveCN104677713BSolve the defect of easy peeling offEasy to take offPreparing sample for investigationPolypideBiochemistry
The invention discloses an improved Grocott silver hexosamine staining method and applications thereof. The staining method comprises the steps of preparing paraffin-embedded sections, staining, carrying out dehydrated hyalinizing, and carrying out section sealing, wherein in the step of preparing paraffin-embedded sections, a sliced paraffin section is adhered to a glass slide subjected to APES treatment. The method overcomes the defect that section shedding easily occurs in the process of staining, especially for frankliniella occidentalis with a small somatotype, the non-section-shedding rate is over 70%. In addition, the internal tissues of polypides in sections obtained according to the staining method disclosed by the invention are red, blastospores in polypides are black, therefore, the polypides are clearly visible, and the staining effect is excellent.
Owner:QINGDAO AGRI UNIV

Transgenosis biocontrol engineering fungus for expressing stomach toxicity insecticidal protein by using endogenous security label and construction method of transgenosis biocontrol engineering fungus

InactiveCN105754882AHigh insecticidal efficacyHigh insecticidal activityBiocideFungiBiotechnologyTransgene
The invention discloses a transgenosis biocontrol engineering fungus for expressing stomach toxicity insecticidal protein by using an endogenous security label and a construction method of the transgenosis biocontrol engineering fungus.Encoding genes of the insecticidal protein Vip3Aa1 are placed under an endogenous strong promoter, and an expression vector with the insecticidal protein genes is constructed.Beauveria bassiana endogenesis ura3 auxotroph is taken as a security label, and competence blastospore, which carries the expression vector, of the beauveria bassiana endogenesis ura3 auxotroph is shifted into a beauveria bassiana wild strain to obtain a recombinant engineering strain of high-expression insecticidal protein Vip3Aa1.The transgenosis biocontrol engineering fungus for expressing the stomach toxicity insecticidal protein Vip3Aa1 by using the non-exogenous resistance label is BbHUV5, and has stomach toxicity insecticidal activity which does not exist in the beauveria bassiana inherently, moreover, the integral insecticidal effect is greatly improved, and environmental risks of any exogenous herbicide resistance gene label do not exist.The engineering strain construction idea and an engineering strain thereof open up a new technological path for a transgenosis fungus insecticide with development synergy and high environment consistency degree.
Owner:ZHEJIANG UNIV

Application of l-proline in increasing the number of blastospores and mycelial biomass of Cordyceps sinensis

ActiveCN110024623BShorten the time of artificial cultivationIncrease biomassCultivating equipmentsMushroom cultivationBiotechnologySpore
The invention discloses the application of L-proline in increasing the number of blastospores and mycelia biomass of Cordyceps sinensis. The present invention aims at the problem of how to increase mycelium biomass in the liquid fermentation process of Cordyceps sinensis and how to obtain a large number of blastospores for infection in the process of artificial bionic culture of Cordyceps sinensis combined with Cordyceps sinensis, adding L-proline to the Cordyceps sinensis culture medium to induce Cordyceps sinensis Bacteria produced blastospores efficiently, and the number of blastospores in the medium added with L-proline increased by 1.49 to 5.26 times after being cultured with Cordyceps sinensis spores for 30 days, and the number of blastospores after 45 days of culture increased by 17 to 52.6 times, After culturing for 60 days, the dry weight of mycelium increased by 15.67-66.44%. The invention can more efficiently obtain blastospores for infecting bat moths for biomimetic culture of insect fungus combined with Cordyceps sinensis, can improve the efficiency of obtaining mycelium by liquid fermentation of Cordyceps sinensis fungus, shortens the time for artificial cultivation of Cordyceps sinensis, and reduces the cultivation cost.
Owner:INST OF ZOOLOGY GUANGDONG ACAD OF SCI

A vector suitable for the genetic transformation and cloning of fungi such as Chinese Mortierella hirsutella sinensis and its construction method

The invention discloses a recombinant plasmid, which is characterized in that promoters, pGpdA and Trpc, in a plasmid pRFHUE-eGFP are respectively replaced by promoters, pAT and pTrpc, in a plasmid of entomogenous fungus. The invention also discloses a preparation method and an application of the recombinant plasmid, and discloses engineering bacteria that include the recombinant plasmid, as well as a preparation method and an application thereof. In the invention, the recombinant plasmid pRF-AETH as well as the engineering bacteria comprising same can convert Hirsutella sinensis, wherein blastospore and mycelia with strong green fluorescence of a conversion strain of the Hirsutella sinensis can be observed under a fluorescence microscope; this phenomenon indicates that in the recombinant plasmid pRF-AETH, an egfp gene can be effectively expressed in the Hirsutella sinensis, so that the recombinant plasmid can be applied to researches on growth, reproduction, development and differentiation processes of the Hirsutella sinensis. The recombinant plasmid supplies support to artificial cultivation of Cordyceps sinensis and has great application prospect.
Owner:成都图径生物科技有限公司

Application of wood vinegar in promoting germination of Esteya vermicola bud spores to generate crescent spores

PendingCN114457000AHas the function of inducing resistanceImprove work efficiencyFungiMicroorganism based processesBiotechnologySporeling
The invention is applicable to the technical field of microorganisms, and provides application of wood vinegar in promoting Esteya vermicola spores to germinate and produce crescent spores, the wood vinegar is applied in the germination stage of the Esteya vermicola spores, the number of the crescent spores produced by the Esteya vermicola spores can be remarkably increased, and particularly, the number of the crescent spores produced by the Esteya vermicola spores can be remarkably increased after the wood vinegar is added into a sterilized water agar culture medium and then the water agar culture medium is poured into the water agar culture medium, so that the yield of the crescent spores produced by the Esteya vermicola spores is increased. And after solidification, coating with a germinated spore suspension of Esteya vermicola, and culturing in a constant-temperature incubator at 25 DEG C for 5 days to obtain a large number of crescent spores. By adding the wood vinegar, Esteya vermicola bud spores can germinate to generate more crescent spores, the mechanism research of killing pine wood nematodes by the crescent spores can be conveniently carried out, the working efficiency of researchers is improved, and the wood vinegar has an induced resistance function, so that the wood vinegar has a good application prospect. And if being applied together with an Esteya vermicola bud spore suspension in the field, the compound has a synergistic effect on prevention and control of the pine wood nematode disease, and has a great application value in the future.
Owner:中国林业科学研究院森林生态环境与自然保护研究所

Preparation method of metarhizium anisopliae conidia microcapsule emulsion

The invention discloses a preparation method of a metarhizium anisopliae conidia microcapsule emulsion. The preparation method comprises the following steps of disinfecting, specifically, disinfectinga culture container firstly, spraying disinfectant fluid, putting in a high-temperature box for high-temperature disinfection, cleaning by using an ultrasonic cleaning box, and drying a container forlater use after cleaning and disinfection; preliminary culture, specifically, selecting the disinfected container according to the volume, placing the metarhizium anisopliae into the container afterselecting the volume, adding hypha diaphragms into the container, carrying out fission so as to generate spore buds in the container, and carrying out culture for 20 hours; and carrying out medium-step culture, specifically, enabling the metarhizium anisopliae conidia to begin to germinate, and transferring protoplast to a bud tube growth poin. The fungus conidium production speed is increased, the fungus culture time and the fungus use space are reduced, the safety and reliability during preparation are improved through multiple reasonable disinfection in the liquid culture stage, the phenomena of atrophy, death and the like of the metarhizium anisopliae are avoided, the reliability is high, and wide popularization can be achieved.
Owner:琼台师范学院

Manufacturing method of permanent slide specimen

PendingCN114235525AEasy to determine the production methodFor long-term storagePreparing sample for investigationSporelingStaining
The invention belongs to the field of slide specimen manufacturing, particularly relates to a permanent slide specimen manufacturing method, and provides the following scheme aiming at the problem that an optimal manufacturing method is difficult to determine when an existing permanent slide specimen is manufactured, the permanent slide specimen manufacturing method comprises the following steps: S1, taking fungal hyphae, spores or germinated spores to manufacture a sample; s2, putting the treated sample into a centrifugal machine, and performing centrifugal treatment; s3, discarding the supernate, and taking the precipitate to prepare a spore suspension; s4, dropwise adding a staining solution on a glass slide, putting the suspended spores into the glass slide, putting a cover glass, sealing the edge of the cover glass by using neutral resin, and marking; s5, putting the labeled slide into a drying oven for drying; and S6, observing the dried slide, and calculating the qualified rate, and according to the method, the qualified rate of multiple batches of permanent slide specimens is calculated and counted, so that the optimal manufacturing method of the permanent slide specimen is conveniently determined.
Owner:江西业力医疗器械有限公司

Vector being suitable for genetic transformation cloning of fungi such as Hirsutella sinensis, and construction method of the vector

The invention discloses a recombinant plasmid, which is characterized in that promoters, pGpdA and Trpc, in a plasmid pRFHUE-eGFP are respectively replaced by promoters, pAT and pTrpc, in a plasmid of entomogenous fungus. The invention also discloses a preparation method and an application of the recombinant plasmid, and discloses engineering bacteria that include the recombinant plasmid, as well as a preparation method and an application thereof. In the invention, the recombinant plasmid pRF-AETH as well as the engineering bacteria comprising same can convert Hirsutella sinensis, wherein blastospore and mycelia with strong green fluorescence of a conversion strain of the Hirsutella sinensis can be observed under a fluorescence microscope; this phenomenon indicates that in the recombinant plasmid pRF-AETH, an egfp gene can be effectively expressed in the Hirsutella sinensis, so that the recombinant plasmid can be applied to researches on growth, reproduction, development and differentiation processes of the Hirsutella sinensis. The recombinant plasmid supplies support to artificial cultivation of Cordyceps sinensis and has great application prospect.
Owner:成都图径生物科技有限公司

Application of juvenile hormone analogue methoxoprim in promoting stiffness of ghost-moth larvae

The invention discloses an application of a juvenile hormone analogue methoxoprim in promoting stiffness of ghost-moth larvae. In order to solve the problems of long artificial culture period, low efficiency and the like of Chinese caterpillar fungus caused by large time consumption in the stiffness process of injected and infected ghost-moth larvae, a juvenile hormone analogue methoxoprim is added to inject and infect the ghost-moth larvae together with cordyceps sinensis blastospores, then the stiffness rate (61.85 + / -3.37%) of theghost-moth larvae is increased, and the stiffness rate is obviously higher than that of larvae only injected with blastospores (48.15 + / -2.69%), so that the stiffness rate of the larvae can be obviously improved by simultaneously injecting the cordyceps sinensis blastospores and the methoxoprim.
Owner:力源新资源开发(广东)有限公司

Application of 20-hydroxy molting hormone in promoting development of ophiocordyceps sinensis blastospores for generating hyphae

The invention discloses application of 20-hydroxy molting hormone in promoting development of ophiocordyceps sinensis blastospores for generating hyphae. For the problems of long time for the ophiocordyceps sinensis blastospores to develop and form mycelia, long cycle and poor efficiency of artificial culture of ophiocordyceps sinensis and the like, the 20-hydroxy molting hormone is added in an ophiocordyceps sinensis culture medium to perform hypha induction culture on the blastospores, it is found that the blastospores germinate to form the hyphae when the culture is performed for 2 days, the blastospores germinate to form long hyphae when the culture is performed for 8 days, and the hypha formation rate is increased by 32.25-171.44% compared with the situation that no inducing substanceis added. The 20-hydroxy molting hormone promotes the growth of the blastospores in the mode of forming the hyphae, and improves the efficiency of the formation of the hyphae through the developmentof the ophiocordyceps sinensis blastospores.
Owner:INNER MONGOLIA FURUI MEDICAL SCI

Hirsutella sinensis transforming strain expressing green fluorescent protein, and preparation method thereof

ActiveCN107418965ASimple materialConvenient for molecular biology researchFungiMicroorganism based processesFunctional genesFluorescent protein
The invention discloses a method for preparing a Hirsutella sinensis transforming strain expressing green fluorescent protein. The method comprises: (1) preparing a Hirsutella sinensis blastospore suspension; (2) preparing Agrobacterium tumefaciens containing recombinant plasmid pRF-AETH; (3) preparing an Agrobacterium tumefaciens liquid; (4) mixing the Hirsutella sinensis blastospore suspension and the Agrobacterium tumefaciens liquid, and co-culturing; and (5) carrying out selective culture, identifying, and obtaining the Hirsutella sinensis capable of stably expressing green fluorescent protein. The invention further provides a Hirsutella sinensis transforming strain. According to the present invention, with the method, the Hirsutella sinensis transforming strain capable of stably expressing egfp gene can be obtained, can provide the convenience for the molecular biology research of the Hirsutella sinensis functional gene, can monitor the growth and reproduction and the development and differentiation of the Hirsutella sinensis in host insects, can provide the direct evidence for the clarification of the interaction between the Hirsutella sinensis and the host, and has excellent application prospects.
Owner:成都图径生物科技有限公司
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