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Manufacturing method of permanent slide specimen

A specimen making and permanent technology, which is applied in the preparation, sampling, and measuring devices of test samples, can solve problems such as difficulty in determining the production method, and achieve the effect of improving the detection rate

Pending Publication Date: 2022-03-25
江西业力医疗器械有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the shortcoming that it is difficult to determine the best production method when making the existing permanent slide specimens, and propose a permanent slide specimen production method

Method used

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  • Manufacturing method of permanent slide specimen

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Embodiment 1

[0026] refer to figure 1 , a permanent slide specimen preparation method, comprising the following steps:

[0027] S1: Take fungal hyphae, spores or blastospores to make samples;

[0028] S2: put the processed sample into the centrifuge and centrifuge;

[0029] S3: Discard the supernatant, and take the sediment to make a spore suspension;

[0030] S4: After dropping the staining solution on the glass slide, put the suspended spores, put the cover glass, use neutral resin to seal the edge of the cover glass, and label it;

[0031] S5: putting the labeled slides into an oven for drying;

[0032] S6: Observing the dried glass slides and calculating the qualified rate.

[0033] In this embodiment, in S1, take fungal hyphae, spores or blastospores and put them into a centrifuge tube, add a treatment solution that has not passed through the spores in the centrifuge tube, and let it stand for 10 minutes to make a sample. Through the treatment solution, the sample can be improved....

Embodiment 2

[0041] refer to figure 1 , a permanent slide specimen preparation method, comprising the following steps:

[0042] S1: Take fungal hyphae, spores or blastospores to make samples;

[0043] S2: put the processed sample into the centrifuge and centrifuge;

[0044] S3: Discard the supernatant, and take the sediment to make a spore suspension;

[0045] S4: After dropping the staining solution on the glass slide, put the suspended spores, put the cover glass, use neutral resin to seal the edge of the cover glass, and label it;

[0046] S5: putting the labeled slides into an oven for drying;

[0047] S6: Observing the dried glass slides and calculating the pass rate.

[0048] In this embodiment, in S1, take fungal hyphae, spores or blastospores and put them into a centrifuge tube, add a treatment solution that has not passed through the spores in the centrifuge tube, and let it stand for 20 minutes to make a sample. Through the treatment solution, the sample can be improved. det...

Embodiment 3

[0056] refer to figure 1 , a permanent slide specimen preparation method, comprising the following steps:

[0057]S1: Take fungal hyphae, spores or blastospores to make samples;

[0058] S2: put the processed sample into the centrifuge and centrifuge;

[0059] S3: Discard the supernatant, and take the sediment to make a spore suspension;

[0060] S4: After dropping the staining solution on the glass slide, put the suspended spores, put the cover glass, use neutral resin to seal the edge of the cover glass, and label it;

[0061] S5: putting the labeled slides into an oven for drying;

[0062] S6: Observing the dried glass slides and calculating the qualified rate.

[0063] In this embodiment, in S1, take fungal hyphae, spores or blastospores and put them into a centrifuge tube, add a treatment solution that has not passed through the spores in the centrifuge tube, let it stand for 30 minutes, and make a sample. Through the treatment solution, the sample can be improved. d...

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Abstract

The invention belongs to the field of slide specimen manufacturing, particularly relates to a permanent slide specimen manufacturing method, and provides the following scheme aiming at the problem that an optimal manufacturing method is difficult to determine when an existing permanent slide specimen is manufactured, the permanent slide specimen manufacturing method comprises the following steps: S1, taking fungal hyphae, spores or germinated spores to manufacture a sample; s2, putting the treated sample into a centrifugal machine, and performing centrifugal treatment; s3, discarding the supernate, and taking the precipitate to prepare a spore suspension; s4, dropwise adding a staining solution on a glass slide, putting the suspended spores into the glass slide, putting a cover glass, sealing the edge of the cover glass by using neutral resin, and marking; s5, putting the labeled slide into a drying oven for drying; and S6, observing the dried slide, and calculating the qualified rate, and according to the method, the qualified rate of multiple batches of permanent slide specimens is calculated and counted, so that the optimal manufacturing method of the permanent slide specimen is conveniently determined.

Description

technical field [0001] The invention relates to the technical field of slide specimen preparation, in particular to a permanent slide specimen preparation method. Background technique [0002] The slide specimen of plant pathogenic bacteria is an important experimental material in the experimental teaching of plant pathology. Plant pathogen slide specimens are divided into permanent slide specimens and semi-permanent slide specimens. Permanent slide specimens can be stored permanently and used permanently. The semi-permanent slide specimens have a short shelf life and can be used for about 3 years. Regarding the method of making permanent slide specimens, in monographs (plant disease research methods) and textbooks (experimental instructions), only the pathogenic bacteria structure together with the host tissue is introduced, such as: ascus with host tissue, conidia How to make permanent glass slide specimens for isolated powdery spores has no introduction, and there is n...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/28G01N1/2813G01N1/30G01N2001/2846
Inventor 熊文芳陈永洪方继利袁书娟陶根金程菊
Owner 江西业力医疗器械有限公司
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