100 results about "Fungal hyphae" patented technology

A method of forming fungal materials and fungal objects from those fungal materials, the method comprising the steps of growing a first fungal tissue in contact with a nutritive vehicle; supplying a porous material in contact with said first fungal tissue; directing growth of said fungal tissue through said porous material such that a portion of said fungal tissue comprises a first fungal material having first fungal hyphae; optionally incorporating composite material; directing a change in the composition or growth pattern of at least some of said first fungal hyphae; separating at least a portion of the first fungal material from said nutritive vehicle; obtaining a second fungal material having second fungal hyphae; and forming a fungal object by encouraging fused growth between said first fungal material and said second fungal material and optionally incorporating composite material.

A method of forming fungal materials and fungal objects from those fungal materials, the method comprising the steps of growing a first fungal tissue in contact with a nutritive vehicle; supplying a porous material in contact with said first fungal tissue; directing growth of said fungal tissue through said porous material such that a portion of said fungal tissue comprises a first fungal material having first fungal hyphae; optionally incorporating composite material; directing a change in the composition or growth pattern of at least some of said first fungal hyphae; separating at least a portion of the first fungal material from said nutritive vehicle; obtaining a second fungal material having second fungal hyphae; and forming a fungal object by encouraging fused growth between said first fungal material and said second fungal material and optionally incorporating composite material.

A method for preparing forage from mulberry twigs includes such steps as pulverizing, mixing it with other raw materials, puffing, middle-or low-temp microbial fermenting, inoculating fungus, fungal fermenting, yeast fermenting, and conventional steps.

The present invention relates to a method for preparation of an environmentally-friendly ecological brick from corncob, the method is as follows: even mixing of a corncob culture raw material, high-temperature sterilization of the corncob culture raw material, inoculation of the corncob culture raw material with a specific fungal species, package of the inoculated culture raw material into a mold for culture for a plurality of days in sterile environment, and drying dehydration processing to obtain the environmentally-friendly ecological brick. The environmentally-friendly ecological brick is prepared from the corn cob, rice husk, bran and other agriculture byproducts as raw materials by use of natural growth of fungal hyphae, mycelium kinking forming and other characteristics by the method, the environmentally-friendly ecological brick has the advantages of being simple in process, low in energy consumption, low in cost, environmentally-friendly, ecological, light in weight and good in strength, and the environmentally-friendly ecological brick is used in interior wall decoration in offices and entertainment buildings, can replace some or all of bricks prepared from asbestos separators and other raw materials, and is a new building material with high market value.

The invention provides a method for identifying pathogen of the paroxysmal foliage wilting disease in myrica rubra, comprising separating the xylem from the phloem of a myrica rubra branch, respectively cutting the phloem and xylem into small sheets of tissue without the surface disinfection, placing them on a PDA flat, culturing in a thermotank of 25 DEG C for 2 days, picking out fluffy fungal hyphae and placing the fungal hyphae to another new PDA flat, continuously culturing in the thermotank of 25 DEG C for 5 days, and picking out purified hypha blocks of 0.5cm<2> for the inoculation experiment of the pathogenicity. The method for testing the pathogen of the paroxysmal foliage wilting disease in myrica rubra in vitro is reliable and fast, needs no expensive materials, comprises simplesteps, and has high accuracy.

The invention relates to a method for preparing a novel biological microcapsule capable of being applied to treatment of ammonia nitrogen and soluble organic substances in water. According to the method for preparing the microcapsule, no chemical crosslinking agent or external material is added as a carrier, but candida is spontaneously combined to an extracellular polymer excreted by fungal hyphae cell aggregation in a natural mode under a special condition, and an appropriate condition is created to induce the intergrowth of hyphomycete and the candida so as to form the biological microcapsule in the shape of a solid microsphere. The novel biological microcapsule can efficiently degrade the ammonia nitrogen and the soluble organic substances in water, can keep the activity of thalli, separates the thalli from the outside poor environment, slows down the influence caused by temperature and pressure, and is favorable for uniform distribution in water, and also favorable for stability in storage and use. The preparation method is simple and feasible, has less influence factors, and has great potential and application value for treating the ammonia nitrogen and the soluble organic substances in the water.

The invention discloses a preparation method of a medical and edible fungus mycelium polysaccharide-polypeptide immune enhancer. The preparation method comprises efficient extraction of mycelium polysaccharide polypeptide based on combination of a physical method and enzymatic hydrolysis, ethanol-based precipitation separation, precipitation purification respectively with levamisole and high-concentration ethanol, and blending preparation of compound immunity-enhancing injection preparations. Through mixing with a vaccine or compounding with an injection, animal immunity is improved, production efficiency is high and a relative cost of production is low. The lactarius deliciosus mycelium polysaccharide is simpler than lactarius deliciosus mycelium monosaccharide and can be further decomposed into rare functional monosaccharide. The preparation method has simple processes.

The invention relates to mucor and application thereof to green brick tea fermentation, and belongs to the field of application of a microbial fermentation technology. The strain is separated from tea leaves with good fungus hyphae growth in the pile-fermentation process of the green brick tea production in a production workshop of a company, and is determined to be the mucor through separation and identification; the mucor is named as xingding-2; the preservation code is CCTCC (China Center For Type Culture Collection) NO: M2017241; the preservation time is May 5, 2017, and belongs to the mucor fungus; the mucor is applied to green brick tea production through fermentation. According to the method for fermenting the green brick tea by the mucor xingding-2 provided by the invention, the mucor xingding-2 is cultured in a liquid state; the fungal spores are collected and are put into the green brick tea fermentation tea piles; the temperature and the humidity are controlled for fermentation. Compared with a conventional green brick tea fermentation method, the green brick tea fermentation method provided by the invention has the advantages that the mouthfeel of the tea product is basically similar; the fermentation period is short; the production rate is greatly improved.

The invention discloses a nutrition additive for improving soil physical and chemical properties during a seedling stage, which is prepared from the following raw materials in parts by weight: 19-21 days of medical stone, 17-19 parts of dried cow dung, 1-3 parts of methionine, 10-14 parts of nitrate of potash, 24-26 parts of water hyacinth powder, 22-24 parts of Chinese medicine residue, 4-5 parts of sodium borate, 12-16 parts of stale tea, 36-40 parts of volcanic, 21-25 parts of threonine mother liquor, 60-65 parts of waste crop straw, 6-7 parts of compound fungal hyphae and proper mount of water. Extracellular glycoprotein generated by macro fungi used in the nutrition additive under culturing of fermented products such as the cow dung and Chinese medicine residue and the fermented residue act together, so formation of a soil granular structure is promoted, stability of culturing soil during seedling stage is improved, soil porosity is increased by matching with mineral materials such as the medical stone, good soil texture and ventilation are ensured, and erosion to the soil by win and rain is resisted, and growth environment of crop during the seedling stage is stabilized.

The present invention discloses a fungal fluorescent staining solution which is simple in operation, good in staining effect, high in detection rate, stable in system and easy to preserve. Fluoresceinin the fungal fluorescent staining solution can bind with beta-polysaccharides on the fungal cell wall with high affinity such as chitin and cellulose so that fungal components in a sample can be labeled and fungal morphology can be clearly observed under a fluorescence microscope. With the addition of an anti-interference fluorescent auxiliary in the staining solution, the binding rate of the fluorescent staining solution with the fungal cell wall is improved; moreover, the fluorescence intensity is enhanced under the ultraviolet excitation so that the propagules such as fungal hyphae and spores emit bright blue-white fluorescence; under the microscope observation, the outline of the fungi is clearly visible while the stability of the staining solution system is improved; and the fluorescence quenching risk of the staining solution is reduced. The staining solution is capable of one-step staining; staining can be completed in a few seconds; the operation is simple; the detection timeis saved; and the detection rate of the fungi is also improved.

The invention belongs to the field of molecular biology study of fungi, and in particular relates to a method for extracting total DNA (deoxyribonucleic acid) of fungal hyphae, which has the advantages of avoiding liquid nitrogen grinding and relatively thoroughly breaking cell walls to extract the total DNA of the fungal hyphae. The method comprises the following steps: (1) collecting the fungal hyphae; (2) putting into a DNA extracting solution, and mashing; (3) repeatedly freeze-thawing for 2 to 3 times in a low temperature environment of -80 DEG C and a water bath environment of 65 DEG C; (4) adding protease K and lysozyme for treating; (5) removing the enzymes and purifying the DNA. The treatment method is simple, and the required agents are common; a repeated freeze-thawing and mashing mode is adopted, so that a sample is prevented from liquid nitrogen grinding, and then the use in places where liquid nitrogen can not be obtained easily is facilitated; a mortar is not used, so that cross contamination is prevented; toxic reagents such as phenol, beta-mercaptoethanol and the like are not used; the whole extracting process is mild, the quality of the extracted total DNA of the fungal hyphae is high, and subsequent molecular biology studies such as PCR (polymerase chain reaction) amplification and sequencing of an ITS (internal transcribed spacer) sequence and the like can be performed.

The invention discloses a seedling-stage nutritional addition material stable in fertilizer efficiency and long in action period. The seedling-stage nutritional addition material is prepared from, by weight, 23 to 27 parts of coal slime, 22 to 24 parts of rice chaff ash, 1 to 2 parts of nitrilotriacetic acid, 10 to 14 parts of sodium nitrate, 16 to 18 parts of sawdust, 17 to 19 parts of chicken feather, 6 to 8 parts of fulvic acid calcium, 36 to 40 parts of volcanic rock, 18 to 20 parts of threonine mother liquor, 21 to 25 parts of aged tea, 72 to 78 parts of waste crop straw, 4 to 5 parts of composite fungal hyphae, and an appropriate amount of water. According to a preparation method, the seedling-stage nutritional addition material is prepared from common raw materials such as coal slime and waste chicken feather via a plurality of steps such as fermentation, chelation, and pelletizing, and is capable of improving soil physicochemical properties and nutritional characteristics; a large amount of fungus is added, so that the seedling-stage nutritional addition material is capable of providing seedlings with required nutrients, relatively stable complementary nutritional system among the bacteria is formed; dominant bacterial communities are formed; and activity of the seedling-stage nutritional addition material can be maintained for a relatively long time, and fertilizer efficiency is achieved.

The invention discloses a salvia miltiorrhiza endophytic fungi hypha water-soluble extract (WSE). The salvia miltiorrhiza endophytic fungi hypha water-soluble extract (WSE) is prepared by following steps: salvia miltiorrhiza endophytic fungi hyphostoma obtained via 9 to 14 days of fermentation is collected; distilled water is added for resuspending; an obtained suspension liquid is sterilized for 30 to 60min at a temperature of 110 to 130 DEG C, and then is subjected to vacuum pumping filtration so as to obtain a filtrate. The invention also discloses polysaccharide protein fractions (PPF) of the salvia miltiorrhiza endophytic fungi hypha water-soluble extract, and polysaccharide fractions (PSF) of the salvia miltiorrhiza endophytic fungi hypha water-soluble extract. The invention further discloses applications of SWE, PPF, and PSF in promoting growth of tissue culture object salvia miltiorrhiza hairy roots of host salvia miltiorrhiza, and increasing tanshinone content of salvia miltiorrhiza hairy roots.

The invention provides a preparation method and application of fungal hypha nitrogen and sulfur self-doped carbon dots and belongs to the field of researches of fluorescent carbon nano-materials. Thepreparation method comprises the following preparation steps: (1) firstly, preparing fungal hyphae by utilizing a biosynthesis technology; culturing the hyphae in a PDA (Potato Dextrose Agar) solid culture medium at constant temperature; then inoculating the hyphae into a modified PDA liquid culture medium and culturing in a constant-temperature oscillator; finally, purifying the hyphae; and (2) taking the hyphae as a raw material and synthesizing the nitrogen and sulfur self-doped carbon dots (N,S-CDs) in one step under a hydrothermal condition. According to the preparation method provided bythe invention, a method combining biosynthesis and the hydrothermal condition is adopted for the first time, and the N,S-CDs with excellent performance are prepared. The N,S-CDs can be used for carrying out high-selectivity and high-sensitivity detection on tetracycline antibiotics (TCs); and when the concentration of TC is 0.5 to 38.46muM, the linear correlation coefficient is 0.991 and the TC detection limit is 0.041muM. The N,S-CDs are used for preparing fluorescence detection test paper and the fluorescence detection test paper can be used for rapidly detecting the TCs in culture wastewater. The N,S-CDs do not influence the survival rate of cells and have a very great application potential in the field of cell imaging.

The invention belongs to the technical field of fungal hypha total DNA extraction, and particularly relates to a fungal hypha total DNA extracting solution and a method for extracting fungal hypha total DNA. The fungal hypha total DNA extracting solution comprises an extracting solution I and an extracting solution II, wherein the extracting solution I comprises a buffer salt, a nonionic surfactant and a neutral salt water solution, and the pH value of the extracting solution I is 7.5-8.5; and the extracting solution II comprises a buffer salt and a cationic surfactant, and the pH value is 7.5-8.5. The fungal hypha total DNA extracting solution simplifies the total DNA extraction steps, is suitable for extracting trace amounts of total DNA, provides an effective experimental means for the scarce experimental materials, avoids the use of the toxic organic solvents, can simplify the operation steps and shorten the time, and avoids the possibility of high tendency to cause frost damage to skin in the liquid nitrogen grinding process.

The invention discloses a nutritional additive used for increasing soil fertility and used in crop seedling period. The nutritional additive is prepared from following raw materials, by weight, 21 to 24 parts of fly ash, 3 to 4 parts of sodium sulfate, 22 to 25 parts of lobster powder, 24 to 26 parts of sugarbeet slag, 2 to 3 parts of tartaric acid, 16 to 20 parts of castor cake, 9 to 11 parts of diammonium phosphate, 58 to 64 parts of waste crop straw, 14 to 16 parts of threonine mother liquor, 22 to 26 parts of aged tea, 4 to 6 parts of composite fungal hyphae, 40 to 44 parts of volcanic rock, and an appropriate amount of water. The nutritional additive is different from common seedling fertilizers; soil aggregate structure is not destroyed by long term using, and accumulation of toxic matters is not caused by long term using; oppositely, adding of fungus and decomposed materials is capable of increasing beneficial microorganism and organic matter content of soil, realizing decomposition of inactive mineral nutrient composition in soil, reducing soil hardening, improving water and nutrient retention capacity in seedling period, improving soil productivity, and promoting growth of seedling crops.

A method fermenting fungal hyphae to highly yield fungal polysaccharides belongs to the technical field of microbial fermentation. The method comprises the following steps: purchasing the following strains: shiitake 881, Pleurotus eryngii 90, Agrocybe cylindracea No.2, shiitake 991 or Dictyophora indusiata D02; culturing a slant preservation medium at 22.5-23.5 DEG C for 4-8 d, and sterilizing a liquid seed medium; performing vibration cleaning on mycelia on the slant preservation medium with 10 mL of normal saline, inoculating a bacterium suspension accounting for 3-8% of the volume of the liquid seed medium, and performing shocking bed culturing under a rotating rate of 170-200 r / min at 23-26 DEG C; and sterilizing a liquid fermentation medium at 115 DEG C, controlling the volume of a liquid in a 500 mL triangular flask to be 100 mL, inoculating with the above obtained seed liquid accounting for 5-8% of the volume of the liquid fermentation medium, uniformly shaking the triangular flask, performing standing culturing for 4-8 h, and performing fermentation on a shaking bed for 4-6 h, wherein the culturing temperature is 23-26 DEG C, and the stirring rotating speed is 180-220 r / min. The fermentation conditions of the fungal polysaccharides are optimized, and water extract accounting for 10-50% of the amount of dried Lauraceae plant powder is added to promote the propagation ofthe fungal mycelia in the fermentation solution, so the biomass amount is increased by 68.45-114.36%, and the yield of extracellular polysaccharides is increased by 68.92-97.91%.

The invention relates to a streptomyces mediolani ZW-1 bacterial strain, and a bacteriostatic application of fermentation broth thereof. Inhibited bacteria comprise wheat fusarium nivale, wheat fusarium graminearum, alternaria leaf spot, sclerotinia sclerotiorum, spinach altemaria solania, helminthosporium carbonum, watermelon fusarium wilt or cotton fusarium wilt. The streptomyces mediolani ZW-1 bacterial strain and the fermentation broth thereof can inhibit the growth of plant pathogenic fungal hyphae, lead to enlargement of cell walls of the pathogenic fungal hyphae and concentration of protoplasts, can inhibit conidial germination and germ tube growth of the spinach altemaria solania, have good bacteriostatic effects, and have good development and application prospects for biological prevention and control of plant diseases.

The invention discloses a method for preparing a mycelium / molybdenum oxide adsorption-catalytic material by utilizing biological enrichment. The method comprises the following steps: preparing a liquid culture medium; adding the liquid culture medium into a culture container, inoculating a fungus strain, carrying out shaking culture, adding an ammonium molybdate solution when the diameter of fungus mycelium pellets in the liquid culture medium reaches 1cm, continuing culture, taking out solids after the culture is finished, washing the solids with deionized water until the solids are neutral,carrying out freeze drying; carbonizing the freeze-dried solids to obtain the mycelium / molybdenum oxide adsorption-catalytic material. The prepared mycelium / molybdenum oxide adsorption-catalytic material can effectively remove tannic acid in a radioactive waste liquid and can effectively reduce U(VI) in the radioactive waste liquid. The invention provides a biomass charcoal / molybdenum oxide composite material prepared on the basis of a fungal mycelium biological enrichment method. The biomass charcoal / molybdenum oxide composite material is used for treating and disposing radioactive wastewatercontaining organic matters.

The invention discloses a method for reducing organic acid content of a fruit wine by adopting fungal hyphae. The method comprises the following steps: adding the fungal hyphae in a vegetative growth stage into the fruit wine in a production process, standing under the condition that the wine temperature is kept at 10-30 DEG C, and intermittently stirring so as to achieve the aim of reducing organic acid. The method disclosed by the invention can be used for reducing the specific organic acid content of the fruit wine by adopting the absorption, decomposition and transformation capacities of the fungal hyphae on the premise that the edible safety is ensured and the flavor can not be influenced, and can achieve a significant effect on reducing the organic acid.

The invention discloses an ITS-RFLP method for rapidly identifying main cotton pathogenic fungus, which mainly comprises the following steps: 1) extracting cotton main pathogenic fungus mycelia genome DNA, which comprises Verticillium dahliae, Verticillium alboatrum, Rhizoctonia solani, Fusarium moniliforme, Trichothecium roseum and Fusarium oxysporum; 2) amplifying ribosome internal transcribed spacer (ITC) using PCR: carrying out rapid amplification using general primer ITS4 and ITS5 of fungi ITS; 3) carrying out enzyme digestion reaction using restriction enzymes and identifying band spectrums: carrying out single digestion reaction of ITS products of cotton main pathogenic fungus randomly using restriction enzymes HhaI, HaeIII, TaqI and Sau3A whose recognition sites are four basic groups, and comparing ITS-RFLP band spectrum correlation tables and standard electronic maps, thereby rapidly identifying classes of pathogens. Compared with traditional time-consuming and labor-consuming morphological and physiological identification as well as ITS clone sequencing identification method of pathogen with high cost, the invention has the advantages of rapid, accuracy and economy, and simultaneous identifications of a plurality of pathogenic fungus.

The invention discloses a paenibacillus polymyxa and application thereof in prevention and treatment of various soil-borne diseases. The preservation number of the paenibacillus polymyxa ZF129 protected by the invention is CGMCC (China General Microbiological Culture Collection Center) No.17631. The paenibacillus polymyxa ZF129 can remarkably inhibit the growth of pathogenic fungus hyphae, shows broad-spectrum antagonistic activity, and has the capability of promoting the growth of plants. The paenibacillus polymyxa ZF129 shows an excellent biocontrol character, has a further development potential, and lays a certain foundation for further study of the paenibacillus on the mechanism of disease prevention.

The invention discloses a method for multi-level value-added utilization of camellia oleifera shells. The method comprises the following steps: firstly, crushing the camellia oleifera shells and screening by a 60-mesh sieve; obtaining tea saponins and tannins from the camellia oleifera shells by an ultrasonic-microwave synergistic stepwise extraction process; inoculating edible fungi after addinga small amount of brans into primary waste residues; raising the temperature and adding lysozyme to promote autolysis of edible fungal hyphae after the fungi grow all over a substrate; releasing cellulase and hemicellulase to obtain prebiotics rich in oligomeric cellulosic polysaccharide, oligomeric pentose and peptidoglycan; preparing secondary camellia oleifera shell waste residues with a high lignin content into an aromatic compound by a low-temperature microwave catalysis rapid-cracking technology; using the aromatic compound after separation and purification as a precursor for producing edible essence; and using residual biochar after steam activation for activated carbon for food industry. The method provided by the invention combines three major industries of edible camellia oleifera oil processing, food additive and biomass refining, proposes a new process of multi-level value-added utilization of the camellia oleifera shells, and can finally realize a new way of efficient value-added utilization of camellia oleifera shell resources without wastes in a whole process.

The invention relates to a fungus-based biomass fireproof material with bagasse as a main material and a preparation method of the fungus-based biomass fireproof material with the bagasse as the main material. The fungus-based biomass fireproof material with the bagasse as the main material is composed of, by mass, 15-55 parts of the bagasse, 20-30 parts of rice hulls, 10-25 parts of rice bran and 15-30 parts of biomass fireproof material additive. The preparation method comprises the steps of preparation of compost, sterilization and inoculated culture. According to the fungus-based biomass fireproof material and the preparation method of the fungus-based biomass fireproof material, the bagasse and other biomass materials are used as the raw materials, the features that fungal hyphae grows rapidly, and mycelia are high in kink capacity are utilized, and the bagasse and other biomass materials are converted into the environment-friendly type biomass fireproof material. The fungus-based biomass fireproof material with the bagasse as the main material is simple in preparation method, low in production cost, high in strength and good in compactness and fireproof performance and has quite good market prospect and potential.

The invention discloses a fungus fluorescence staining method. The method includes firstly, spraying a staining solution [dissolving direct yellow 96 in a 0.1M Tris-Hcl buffer solution with the pH (potential of hydrogen) of 8.5] on the surfaces of host plant leaves uniformly directly to observe conditions of pathogenic fungal spores on the surfaces of the host plant leaves; secondly, soaking leaves decayed by pathogenic fungal hyphae into 95% (v / v) ethyl alcohol in boiling water bath to remove chlorophyll completely; thirdly, taking out the leaves, and cleaning the leaves by 50% (v / v) ethyl alcohol, 50mM sodium hydroxide and pure water sequentially; finally, soaking the leaves into the staining solution to stain so as to observe the conditions after pathogenic fungi invade host leaf tissues. The fungus fluorescence staining method has the advantages that all reagents used in the method are poisonless and harmless and are safer and more environment friendly than reagents such as chloroform, phenol, trichloroacetic acid and pyridine used in conventional decoloration methods; the fungus fluorescence staining method is simple and feasible, and a slicing process is omitted and staining steps are simplified on the premise of not affecting the staining effect.

The invention relates to cooked wheaten food made by utilizing medical edible fungal hypha material and a method thereof. The cooked wheaten food is directly made by taking medical edible fungal hypha material, flour, fruit and vegetable juice, egg, milk, sugar, salt and ferment powder as raw materials, combination of abundant nutrient component and curative effect functional component in the medical edible fungal hypha material as well as flour with high energy, low protein and single function is fully utilized, nutrient and function complementary cooked wheaten food is made, thus common cooked wheaten food becomes the cooked wheaten food which is full of nutrients, is unique in flavour and has multiple effects of regulating and improving organism function and improving immunity, and the cooked wheaten food is used as daily life staple food and is important to disease resistance health care and life prolonging of human body.

The invention belongs to the field of biological material recycling, and particularly relates to a mushroom bran recycling method. In the method, mushroom bran, straw, corncobs, bagasse, rice straw and other forestry and agricultural residues serve as the raw materials, with the aid of a nutrient solution, by means of the characteristics of rapid growth of fungal hyphae and strong twisting capacity of mycelia, the mushroom bran and the forestry and agricultural residues are converted into a mycelium composite, and the composite has the advantages of being light in weight, low in cost and capable of being degraded, so the resourceful utilization of the mushroom bran is realized, and the mushroom bran can be used for the fields of light packaging and transporting, is excellent in compressingand buffering performance, can relieve the environment air pollution and improve economic benefits, is an environment-friendly material with great market economic value and can be applied in the field of transporting and packaging.

The invention relates to the field of application of biological materials, and discloses application of a degradable composite material in oil absorption, and the degradable composite material comprises a three-dimensional network structure formed by fungal hyphae and at least one lignocellulose fragment fixed by the three-dimensional network structure. The degradable composite material has good oil absorption performance and has good application potential in the aspects of petroleum leakage, removal of oil stains on water, treatment of oily wastewater and the like. On the other hand, the problem of treatment of agricultural residues such as straw and wood chips and forestry residues can be solved, the additional value of the residues is increased, environmental pollution is reduced, and coordinated development of economy, ecological environment and society is facilitated.

The invention provides a non-toxic coloring agent used for detecting grass endophytic fungi. The non-toxic coloring agent comprises aniline blue, lactic acid and solvent. The non-toxic coloring agent is free from phenol harmful to human beings and environment, coloring effect is improved by simply heating slides on alcohol lamp flame during coloring, and distinguish between endogenous fungal hyphae and cell walls is facilitated.