49results about How to "Good cell shape" patented technology

The invention relates to an improved mesenchyme stem cell protection solution as well as application and a preparation method thereof. The protection solution can effectively prolong the activity remaining time of mesenchyme stem cells, reduces preparation cost, and has the advantages of wide raw material source, simple preparation, safe and reliable direct clinical application; and after the mesenchyme stem cells are preserved for 48 hours in the protection solution, the cell activity is still above 90 percent, the cell morphology is normal, and the multiplication capacity and mesenchyme stem cell phenotype characteristics are not influenced.

A serum-free fibroblast culture medium and a preparation method thereof, the basal medium of the present invention is DMEM medium or its mixing with F12 medium; exogenous additives include hormones, metalloproteins, glutamine, lipids Concentrates, antioxidants, purines, sugars and cell growth factors. Compared with the prior art, the present invention has the following advantages: no animal-derived or recombinant albumin is added, which avoids possible safety hazards and reduces the cost of the medium; it is suitable for the primary and subculture of fibroblasts, and maintains a good The cell morphology is similar to that of the cells in the serum group; it does not need to be coated with adhesion factors, the adhesion rate of the primary 24h is > 60%, and the adhesion rate of the subcultured 24h is > 80%, which is no different from that of the cells in the serum group; It meets the needs of multiple passages, and the growth status remains similar to that of serum group cells after 15 passages; the fibroblasts are directly transferred from the serum-containing culture to the culture of the present invention, and the cells can adhere to the wall and extend smoothly without gradually adapting to the process .

The invention relates to a method for preparing an edible mushroom packaging material from laccase modified water lettuce fiber, and belongs to the technical field of packaging materials. The method comprises the following steps: firstly, preparing water lettuce fiber from water lettuce leaves by using processes such as boiling, acid extraction, alkali extraction and the like, dissolving carboxymethyl chitosan and xanthan gum into water, mixing with methanol so as to obtain a mixed liquid, modifying the water lettuce fiber with laccase, performing a reaction with the mixed liquid, concentrating after the reaction so as to obtain a concentrated liquid, mixing with montmorillonite, sorbitol and succinylated monoglycerides so as to obtain a viscous fluid, defoaming, performing membrane blowing, and cutting, thereby obtaining the edible mushroom packaging material. The edible mushroom packaging material prepared by using the method not only is capable of inhibiting propagation and reproduction of malignant bacteria, enabling the decomposition function of edible mushroom to be in a lowest state and prolonging the freshness preservation time, but also is of a micropore structure, effectively retards moisture loss, keeps a relatively good cell morphology, and is convenient and safe to use.

The invention discloses a micro-foaming polyethylene-matrix wood-plastic composite material and a preparation method thereof. The micro-foaming polyethylene-matrix wood-plastic composite material is prepared by the following steps of stirring a matrix polyethylene resin, modified wollastonite powder and talcum powder, a crosslinking agent, a lubricant, a heat balance type foaming agent, an antioxidant, an anti-ultraviolet agent, pigment and modified plant fiber, heating and uniformly mixing, then cooling and discharging to obtain a premix, wherein the modified plant fiber is one of wood powder, bamboo powder and rice hull powder or a mixture of two or more at any proportion; putting the premix into an extruder, and performing granulation, extrusion foaming and cooling formation. The micro-foaming polyethylene-matrix wood-plastic composite material disclosed by the invention is convenient to produce and manufacture, the raw materials are cheap and environment-friendly, the average diameter of the pores of the micro-foaming polyethylene-matrix wood-plastic composite material is reduced to 10 microns, and the content of the plant fiber is increased to 45%; moreover, the composite material has low density and high bending strength and impact toughness, is widely applicable and can be applied to the fields such as decoration, building, municipal administration, packaging, automotive upholsteries and the like.

The invention relates to a method for culturing pluripotent stem cells. The method adopts a 48-pore plate as a culture container, the cell inoculation density is 4.5-6.5 * 10 < 5 > cells / mL, the culture volume is 0.4-0.6 mL, and the rotating speed of a shaking table is 170-190 rpm. According to the method, a 48-pore plate suspension culture system suitable for growth and differentiation of the pluripotent stem cells is established from nothing to thing, and the culture system can be used for efficiently screening a differentiation inducer of the pluripotent stem cells. The culture method provided by the invention has the advantages of low cost, high efficiency, capability of maintaining better cellular morphology, stable proliferation multiple, capability of maintaining better karyotype and phenotype, smaller difference between experiments in the differentiation process and the like.

The invention relates to the field of medicines, in particular to a composition and an application thereof, a placenta preservative and a preparation method of the placenta preservative. Umbilical cord plasma is adopted as a nutrient of a placenta-derived mesenchymal stem cell preservation solution for the first time, so that multi-element nutrient substances are provided for seed cells; the cell viability is maintained; and the original cell morphology and biological characteristics of placenta-derived mesenchymal stem cells can be well kept. Due to the addition of reducing agents such as N-acetylcysteine and vitamin C, and an antioxidant, the resistivity of the placenta-derived mesenchymal stem cells is greatly improved; and the vitality of the placenta-derived mesenchymal stem cells can still be well kept within 24 hours.

The invention belongs to the technical field of medicine, and the invention provides a new application of rosin, especially the application of rosin in the preparation of drugs for reducing the toxic and side effects of cytotoxic chemotherapeutic drugs. Experiments show that rosin can improve the cell survival rate of cells damaged by cytotoxic chemotherapy drugs, improve the cell morphology of cells damaged by cytotoxic chemotherapy drugs, and improve the organ damage caused by cytotoxic chemotherapy drugs. At the same time, the combined use of rosin and cytotoxic chemotherapeutic drugs will not weaken the antitumor efficacy of cytotoxic chemotherapeutic drugs. It shows that rosin can reduce the toxic and side effects of cytotoxic chemotherapy drugs, improve the tolerance of cancer patients to chemotherapy, improve the quality of life of cancer patients, enhance their physical fitness, and prolong their survival.

The invention relates to a method for separating and purifying oligodendrocyte precursor cells of the cerebral cortex of tupaia belangeri in vitro, and belongs to the technical field of cell separation and purification. According to the method, trypsin and DNase I are combined to digest the cerebral cortex, and the OPCs of tupaia belangeri are well separated and purified by conducting differentialadherence purification twice. The method is simple in operation step, has the advantages of being quick in cell adherence, high in survival rate and the like, has the extremely high cell purity in asubsequent identification experiment and the high reliability of an identification result, can meet the requirements for relevant experimental research on tupaia belangeri in various research fields of medicine, biology, pharmacy and life science, and is suitable for application and popularization in general laboratories.

The invention discloses a placenta preservation method, placenta preservation fluid and a placenta preservation fluid preparing method. The placenta preservation fluid contains cord plasma and / or glucose, penicillin-streptomycin and low molecular dextran-40. The placenta preservation fluid preparing method comprises the step of adding 3-5 v / v% of cord plasma, 5 m / v% of glucose solution, 1X-3X of penicillin-streptomycin and low molecular dextran glucose injection to PBS, wherein the final concentration of low molecular dextran-40 is 1 m / v%. According to the placenta preservation method, placentas are soaked in the placenta preservation fluid to be preserved in a dark place at 2-4 DEG C. An ion buffer system and a nutrition system of the preservation fluid are improved, constituents and content of constituents in the preparation are optimized, long-time preservation of in vitro placentas can be achieved, the vigor of placental mesenchymal stem cells can be well maintained, and functional variation of cells is avoided.