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Improved mesenchyme stem cell protection solution and application thereof

A technology of mesenchymal stem cells and protection solution, applied in the field of improved mesenchymal stem cell protection solution, which can solve the problems of short time and limited application range of mesenchymal stem cells

Active Publication Date: 2010-12-22
青岛奥克生物开发有限公司
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AI Technical Summary

Problems solved by technology

[0007] Another disadvantage of the prior art is that it maintains the viability of mesenchymal stem cells for a short period of time. Mesenchymal stem cells can maintain more than 90% of their viability in the cell preservation solution of the prior art for only 24 hours, and with time Prolonged, the cell viability decreases, which means that the cell viability will drop below 90% after 24 hours. However, stem cell therapy and clinical trials have been carried out in most cities in China and many foreign countries. Using existing means of transportation, 24 hours The viability maintenance time of mesenchymal stem cells obviously limits the application range of mesenchymal stem cells

Method used

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  • Improved mesenchyme stem cell protection solution and application thereof
  • Improved mesenchyme stem cell protection solution and application thereof
  • Improved mesenchyme stem cell protection solution and application thereof

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Experimental program
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Embodiment Construction

[0027] 1. Isolation, culture and phenotype identification of umbilical cord mesenchymal stem cells

[0028] (1) Soak the umbilical cord in sterile PBS buffer (Solarbio) containing 100IU / L penicillin and 100mg / L streptomycin for 10-15 minutes.

[0029] (2) Fully rinse the umbilical cord with the above sterile PBS buffer containing penicillin and streptomycin, and cut the umbilical cord tissue as much as possible into 1mm 3 Small pieces.

[0030] (3) Add the cut tissue to DMEM medium (Gibco, USA) to wash, centrifuge at 2000 rpm for 10 minutes, and discard the supernatant.

[0031] (4) Add trypsin (Sigma, USA) to DMEM medium to prepare a digestion solution with a final concentration of 0.25% (volume ratio), suspend the tissue block in the digestion solution, and place it in a 37°C incubator for 30 minutes digestion , Centrifuge, and discard the supernatant.

[0032] (5) Add DMEM medium to suspend the tissue pieces, inoculate all the tissue pieces into cell culture dishes, and place them a...

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Abstract

The invention relates to an improved mesenchyme stem cell protection solution as well as application and a preparation method thereof. The protection solution can effectively prolong the activity remaining time of mesenchyme stem cells, reduces preparation cost, and has the advantages of wide raw material source, simple preparation, safe and reliable direct clinical application; and after the mesenchyme stem cells are preserved for 48 hours in the protection solution, the cell activity is still above 90 percent, the cell morphology is normal, and the multiplication capacity and mesenchyme stem cell phenotype characteristics are not influenced.

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to an improved mesenchymal stem cell protective solution, application and preparation method thereof. technical background [0002] Mesenchymal stem cells (mesenchymal stem cells, MSCs) are a type of mesenchymal stem cells with multi-differentiation ability. They are widely present in a variety of tissues throughout the body. They can be cultured and expanded in vitro and can be differentiated under specific conditions. Cardiomyocytes, nerve cells and fat cells, etc. With the in-depth study of the biological characteristics and functions of mesenchymal stem cells by domestic and foreign scholars, it is now possible to isolate mesenchymal stem cells from a variety of tissues. Mesenchymal stem cells have the functions of supporting hematopoiesis and regulating immunity, and have broad clinical application prospects in hematopoietic reconstruction, tissue repair, and immunotherapy. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 魏斯溧高宏王丽胡建霞张学峰张美荣
Owner 青岛奥克生物开发有限公司
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