39results about How to "Characteristics unchanged" patented technology

An outer rotor motor is miniaturized, is easier to assemble, and lead ends can be insulated with improved reliability without a drop in motor characteristics. One or a plurality of concave channels provided in the axial direction in a circumferential surface of a housing and one or a plurality of rotation-stopping portions that engage the concave channels are provided in a surface of each coil bobbin that faces the cylinder. Through-holes are provided in the rotation-stopping portions in parallel with the rotor shaft.

The invention discloses a method for in-situ cryopreservation of cells by a three-dimensional micro-carrier. The method includes the following steps: (1) centrifuging a three-dimensional micro-carriersuspension of cells, and discarding a supernatant to obtain a carrier containing cells; (2) mixing the carrier containing the cells with a cryopreservation solution, and then adding the mixture intoa cryopreservation tube; (3) cooling the cryopreservation tube added with the carrier containing the cells in the step (2), and then transferring to liquid nitrogen to realize in-situ cryopreservationof the cells by the three-dimensional micro-carrier. The method can realize in-situ cryopreservation of cell culture on the three-dimensional micro-carrier without separating the cells from the micro-carrier and preforming cryopreservation, realizes that cell cryopreservation does not interrupt the three-dimensional culture process, is convenient for users to store, transport and resuscitate thecultured three-dimensional cell products, and is further convenient for long-term preservation of the cells.

The invention relates to an improved mesenchyme stem cell protection solution as well as application and a preparation method thereof. The protection solution can effectively prolong the activity remaining time of mesenchyme stem cells, reduces preparation cost, and has the advantages of wide raw material source, simple preparation, safe and reliable direct clinical application; and after the mesenchyme stem cells are preserved for 48 hours in the protection solution, the cell activity is still above 90 percent, the cell morphology is normal, and the multiplication capacity and mesenchyme stem cell phenotype characteristics are not influenced.

The invention relates to a gas-liquid mixed transportation supercharging system. The gas-liquid mixed transportation supercharging system comprises a solid filtering part, a gas liquid separation part and a compression storage part, wherein the solid filtering part is arranged at the front end of the gas-liquid separation part and communicated with a mixed transportation inlet, and is used for filtering gas-liquid mixed transportation components entering from the mixed transportation inlet; the gas-liquid separation part is arranged behind the solid filtering part, an inlet of the gas-liquid separation part is communicated with an outlet of the solid filtering part to receive the filtered gas-liquid mixed transportation components; and the compression storage part is arranged behind the gas-liquid separation part and then s used for compressing gas and storing liquid. As a result, the gas-liquid mixed transportation supercharging system guarantees high-pressure gas-liquid mixed transportation components at the outlet of a pipeline through filtering, separation and supercharging of low-pressure gas-liquid mixed transportation components at the inlet of the device, ensures the continuity of gas-liquid mixed transportation, is suitable for long-distance transportation of shale gas, coal bed gas, wellhead gas and the like currently exploited in China, and is environment-friendly and convenient to maintain.

The invention discloses amphiploid histidine auxotroph saccharomyces cerevisiae and a constructing method thereof, belonging to the field of microorganism biological engineering. The saccharomyces cerevisiae is made from an amphiploid saccharomyces cerevisiae bacterial strain which is TSH-Sc-001(His3- / -) and contains a histidine auxotrophic mark and a g418 resistance mark. The constructing methodof the amphiploid histidine auxotroph saccharomyces cerevisiae comprises the following steps: the amphiploid saccharomyces cerevisiae bacterial strain is adopted as a start bacterial strain, the start bacterial strain is transformed twice by a segment with target genes, and the amphiploid histidine auxotroph saccharomyces cerevisiae bacterial strain with two chromosomes being subjected to homologous recombination is obtained by two-time homologous recombination. The saccharomyces cerevisiae is easily identified and protected; in addition, the constructing method of the amphiploid histidine auxotroph saccharomyces cerevisiae provides a novel way and a method for the reconstruction of gene engineering of amphiploid or multiploid saccharomyces, is simple and highly efficient, and keeps the primary characteristic of the saccharomyces unchanged.

The invention provides a method for tissue culturing and fast propagating Primula poisonii, comprising explant selection, surface disinfection, adventitious bud induction, subculture, rooting culture and test-tube seedling transplanting. The method is characterized in that one axillary bud can induce about 40-50 sprouts, the multiplication coefficient is about 3- 4 after 30- 40 days subculture, the rooting rate reaches above 95%, and the replanting survival rate is nearly 100%. The invention realizes preservation for single Primula poisonii, meanwhile, greatly increases seedling capacity for the Primula poisonii, reduces seedling time and cost, and provides technique support for Primula poisonii application in large areas.

A producing and processing technology for an optical module shell includes the steps that die-cast formation is conducted on the optical module shell through a zinc alloy, and surface nickel plating is conducted, wherein the thickness of a cover plate ranges from 0.4 mm to 0.55 mm, and the thickness of a base ranges from 0.6 mm to 0.8 mm; oxide and impurities on the surface of the cover plate and the surface of the base are removed; the base is placed in a clamp, and the space between the base and the cover plate is coated with soldering flux, wherein the thickness is 0.02 mm; the cover plate is placed on the base without dislocation; the cover plate is pressed downwards with force so that the cover plate, the soldering flux and the base can make close contact; the residual soldering flux is wiped off, and cleanness is kept; it is ensured that the surface of a seam welding electrode is clean and dry; the inner hole wall of the seam welding electrode is coated with conductive grease with the thickness being 0.02 mm, the seam welding electrode is installed on a parallel seam welding machine in the manner that the seam welding electrode can slightly rotate, and the whole seam welding electrode is kept clean; one point is formed at the position of the contact center of cover plate and the base through seam welding, the seam welding electrode is moved to the rightmost end or the leftmost end, and seam welding is started; and a qualified product is obtained. The producing and processing technology solves the problems that the production cost of the optical module shell is high and during packaging, the gas tightness and firmness are poor and a clad layer is damaged.

The invention discloses a method for in-situ cryopreservation of cells by a three-dimensional micro-carrier. The method includes the following steps: (1) centrifuging a three-dimensional micro-carriersuspension of cells, and discarding a supernatant to obtain a carrier containing cells; (2) mixing the carrier containing the cells with a cryopreservation solution, and then adding the mixture intoa cryopreservation tube; (3) cooling the cryopreservation tube added with the carrier containing the cells in the step (2), and then transferring to liquid nitrogen to realize in-situ cryopreservationof the cells by the three-dimensional micro-carrier. The method can realize in-situ cryopreservation of cell culture on the three-dimensional micro-carrier without separating the cells from the micro-carrier and preforming cryopreservation, realizes that cell cryopreservation does not interrupt the three-dimensional culture process, is convenient for users to store, transport and resuscitate thecultured three-dimensional cell products, and is further convenient for long-term preservation of the cells.

The invention provides a device and method for repairing broken water pipes using magnetic fluid, which includes an electromagnet prefabrication device, the interior of the electromagnet prefabrication device is filled with magnetic fluid and hardener; the iron core of the electromagnet prefabrication device is A wire is wound, and the wire is connected to the portable power supply through a sliding rheostat. This method is mainly aimed at the ruptured water pipes in the city that are exposed outside the surface and have a great impact on the lives of users. The quick and easy operation can not only repair the ruptured water pipes quickly, but also will not affect the normal life of users; the magnetic fluid with better stability is used , It also better increases the service life of the water pipe after repair; the device can be recycled and reused after the water pipe is repaired.

The invention discloses a human thymoma cell line and application thereof, belonging to the field of tumor biology. The human thymoma cell line is named Thy0517 and has a collection number of CGMCC (China General Microbiological Culture Collection Center) No.9328. The human thymoma cell line Thy0517 has main advantages of having protein expression similar to primary tumor tissues, abnormal karyotype and characteristics of tumor cells with in-vitro tumor forming capability. After in-vitro continuous passage culture, the characteristics of thymoma cells keep unchanged, and therefore the human thymoma cell line can be used as a cell material for researching and developing thymoma-related drugs. EGFR (Epidermal Growth Factor Receptor) expression of the human thymoma cell line is positive, so that proliferation, angiogenesis, adhesion, invasion and metastasis of the tumor cells can be promoted; the cell line can be used as a powerful cell tool for researching EGFR-targeted drugs.