The invention provides an anther culture method of primula forbesii, comprising selection of the explant, disinfection and vaccination of the explant, induction and proliferation of the callus, differentiation of the callus, and rooting culture and transplantation of the tissue culture seedling, wherein, the medium of the induction and proliferation of the callus is as follows: MS +6- BA1.0mg / L +2,4-D 0.5mg / L, 30g / L sucrose, 6g / L agar, pH 5.9-6.0; the differentiation medium of the callus is as follows: MS +6- BA 0.2mg / L + NAA 0.01mg / L, 40g / L sucrose, 7g / L agar, pH 5.9-6.0; the rooting medium is as follows: MS, pH 5.9-6.0. The seedling obtained through the method provided by the invention has good growth, strong consistency and adaptability, less pests and diseases, easy management, and is easy for scale production.