Tissue culture method for fast propagation of primula poissonii

A technique for tissue culture rapid propagation and sea bream, which is applied in the field of plant tissue culture, can solve the problems of no tissue culture of rhizoma rhizoma rhizoma, the method of division propagation cannot meet production requirements, etc., and achieves shortening of seedling cycle and strong consistency. , the effect of dark green leaves

Inactive Publication Date: 2011-05-11
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to seed propagation, the available methods for asexual propagation of Primula are: branch (leaf cluster) propagation, tissue culture propagation, and a small number of perennial species can be propagated by cuttings, but the method of branch propagation is far from meeting production needs.
[0003] Tissue culture is an important way to rapidly propagate good plant varieties. Many species of Primula have been reported on tissue culture, but there is no relevant research and report on the tissue culture of Primula (Primulapoisonii), so it needs to be studied. Tissue culture of Primula poisonii (Primula poisonii) and a set of specialized rapid propagation technology system

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Use Primula poisonii leaves as explants to explore the optimal medium for adventitious bud induction: Choose well-growing seedling leaves as explants to explore the best medium formula for adventitious bud induction. Take the young leaves of Primula poisonii and rinse them with tap water for 2 to 3 hours. Drop a drop of detergent in the water and shake for 15 minutes to remove surface impurities. Repeatedly rinse with tap water; surface disinfection: 70% alcohol for 10 to 15 seconds, sterile water Rinse 4 to 5 times, disinfect with 0.1% mercury liters for 3 to 4 minutes, and then rinse with sterile water for 4 to 5 times. Then cut the sterilized leaves into 1cm 2 Small pieces (with part of the veins) inoculated on the induction medium for clumping buds, 5-6 pieces per bottle, 10 bottles of each medium, and dark culture. Among them, the concentration gradient of 6-BA: 0.5mg / l, 1.0mg / l and 2.0mg / l; the concentration gradient of NAA: 0.05mg / l, 0.1mg / l and 0.5mg / l, the pH of...

Embodiment 2

[0027] Use Primula poisonii axillary buds as explants for primary culture and subculture: select the axillary buds on the vigorously growing plants, rinse them with tap water for 2 to 3 hours, and inoculate the leaves in the same sterilization process as MS+6- BA 2.0mg / l+NAA 0.1mg / l clumping bud induction medium, inoculate 2~3 per bottle; the light conditions are 3000 Lux of natural scattered light plus artificial auxiliary light source 1800±200 Lux, and the light time is 14 h.

[0028] When the axillary buds are inoculated on the differentiation medium, the new leaves are continuously drawn out. Starting from 10 days, the petioles of the extracted new leaves are thick, the base becomes red, and light green callus is gradually formed. Adventitious buds begin to differentiate after 2 weeks; 40 days after inoculation , On the newly extracted petioles and leaves of axillary buds, a large number of adventitious buds are differentiated.

[0029] The statistical results show that after 2...

Embodiment 3

[0031] Rooting culture of tissue-cultured seedlings of Primula poisonii: When adventitious buds grow 2 to 3 leaves on the differentiation medium, they are excised from the callus and transferred to the rooting medium. Formula: MS , MS+NAA (0.1mg / l, 0.2mg / l, 0.5mg / l), the pH is 5.8; the light conditions are 3000 Lux of natural scattered light plus 3000 Lux of artificial auxiliary light source, and the light time is 14h.

[0032] From the point of view of the time required for rooting, the shortest time required for rooting in the medium MS and MS+0.2mg / l NAA is 8-10 days, but in MS+0.2mg / l NAA, the rooting amount of tissue cultured seedlings And growth is obviously better than MS basic medium, the average height of tissue cultured seedlings can reach 4.8cm, and the rooting rate is over 95%.

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Abstract

The invention provides a method for tissue culturing and fast propagating Primula poisonii, comprising explant selection, surface disinfection, adventitious bud induction, subculture, rooting culture and test-tube seedling transplanting. The method is characterized in that one axillary bud can induce about 40-50 sprouts, the multiplication coefficient is about 3- 4 after 30- 40 days subculture, the rooting rate reaches above 95%, and the replanting survival rate is nearly 100%. The invention realizes preservation for single Primula poisonii, meanwhile, greatly increases seedling capacity for the Primula poisonii, reduces seedling time and cost, and provides technique support for Primula poisonii application in large areas.

Description

Technical field [0001] The present invention relates to plant tissue culture, in particular to a method for rapid propagation of Primulapoisonii tissue culture. Background technique [0002] Primula poisonii (Primula poisonii) is a plant of the genus Primula of the Primula family, which is a very beautiful garden ornamental flower. Primula poisonii (Primula poisonii) is a perennial herbaceous flower. The entire plant is in the shape of a rosette with a well-developed rhizome. It grows 5 or more leaf clumps. It is mainly planted and reproduced. Generally, after planting in spring, it grows vegetatively. Flower begins in May of the year, grows slowly, and has a low reproduction coefficient. In addition to sowing propagation, the methods available for vegetative propagation of Primula include: ramets (leaf clumps) propagation, tissue culture propagation. A few perennial species can be propagated by cuttings. However, ramets propagation methods are far from meeting production needs....

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04A01G7/00A01G31/00
Inventor 张启翔李翠娟潘会堂梁树乐程堂仁孙明
Owner BEIJING FORESTRY UNIVERSITY
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