The invention discloses a
tissue culture and intermediate propagation method for succulent
Haworthia cooperivar. pilfera M. B. Bayer. The method comprises the following steps: selecting
Haworthia cooperivar. pilfera M. B. Bayer leaves in a good growing state as explants, after disinfecting the explants, inoculating the disinfected explants to clustered
shoot inducting medium for inducing clustered shoots, then placing the clustered shoots into a rooting medium for taking roots, or after subculturing of the clustered shoots, placing the clustered shoots into the rooting medium for taking roots, and then carrying out
seedling adaptation and
plant transplanting on intact plantlets after root growing. According to the method,
Haworthia cooperivar. pilfera M. B. Bayer seedlings can be obtained by tissue culturing to Haworthia cooperivar. pilfera M. B. Bayer leaves, the material used in the method is easy to obtain, the disinfecting is convenient, the
survival rate is high, the production period is short, the propagation coefficient and the propagation speed of the Haworthia cooperivar. pilfera M. B. Bayer are improved, the strain advantages of the Haworthia cooperivar. pilfera M. B. Bayer can be well kept, a great amount of excellent Haworthia cooperivar. pilfera M. B. Bayer seedlings suitable for
transplanting can be propagated quickly, the production demands are met, and the
economic benefits of planters are improved.