Special culture medium for improving tissue culture propagation speed of haworthia succulent plants and tissue culture method
A technology for succulents and culture medium, which is applied in the field of special medium for increasing the reproduction speed of tissue culture of succulents belonging to the genus Twelve succulents, can solve the problems of low reproduction rate of tissue culture and the like, so as to increase the reproduction speed, improve the speed of product launch, and reduce the The effect of tissue culture cost
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Embodiment 1
[0020] 1. Selection and disinfection of explants:
[0021] The leaves of the well-grown plants to be cultured are washed with clear water, soaked in a carbendazim solution with a mass content of about 5% for 10-15 minutes, and then rinsed with clear water.
[0022] Put 1% mercuric chloride solution in a sterile bottle on the ultra-clean workbench for 3-5 minutes (determine the length of soaking time according to the degree of tenderness and pollution), pour out the mercuric chloride solution, and use a cooled sterile Rinse with water 3-6 times, put into a sterile petri dish, cut into 1×1cm with a sterile blade 2 The size of the leaves, and scratch the back of the leaves, to be inoculated.
[0023] 2. Inoculation and establishment of sterile lines:
[0024] Place the cut leaves flat on the surface of the sterile culture medium with a sterile camera, touch the culture medium downward on the side that crosses the wound, tighten the mouth of the sterile medium bottle, mark it, a...
Embodiment 2
[0028] 1. Selection and disinfection of explants:
[0029] The leaves of the well-grown plants to be cultured are washed with clear water, soaked in a carbendazim solution with a mass content of about 5% for 10-15 minutes, and then rinsed with clear water.
[0030] Put 1% mercuric chloride solution in a sterile bottle on the ultra-clean workbench for 3-5 minutes (determine the length of soaking time according to the degree of tenderness and pollution), pour out the mercuric chloride solution, and use a cooled sterile Rinse with water 3-6 times, put into a sterile petri dish, cut into 1×1cm with a sterile blade 2 The size of the leaves, and scratch the back of the leaves, to be inoculated.
[0031] 2. Inoculation and establishment of sterile lines:
[0032] Place the cut leaves flat on the surface of the sterile culture medium with a sterile camera, touch the culture medium downward on the side that crosses the wound, tighten the mouth of the sterile medium bottle, mark it, a...
Embodiment 3
[0036] 1. Selection and disinfection of explants:
[0037] The leaves of the well-grown plants to be cultured are washed with clear water, soaked in a carbendazim solution with a mass content of about 5% for 10-15 minutes, and then rinsed with clear water.
[0038] Put 1% mercuric chloride solution in a sterile bottle on the ultra-clean workbench for 3-5 minutes (determine the length of soaking time according to the degree of tenderness and pollution), pour out the mercuric chloride solution, and use a cooled sterile Rinse with water 3-6 times, put into a sterile petri dish, cut into 1×1cm with a sterile blade 2 The size of the leaves, and scratch the back of the leaves, to be inoculated.
[0039] 2. Inoculation and establishment of sterile lines:
[0040] Place the cut leaves flat on the surface of the sterile culture medium with a sterile camera, touch the culture medium downward on the side that crosses the wound, tighten the mouth of the sterile medium bottle, mark it, a...
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