49results about How to "Reduce the cost of tissue culture" patented technology

The invention relates to an open type method for cultivating toxin-free seedlings of sugarcanes, comprising the following steps: processing the stems with hot water; preparing the anti-bacterial agent; disinfecting the ware; inoculating; executing the inducing cultivation, the propagation cultivation and the rooting cultivation; and transplanting from seedling bottles. The open type method for cultivating the toxin-free seedlings of the sugarcanes of the invention needs not to sterilize the culture medium at high temperature, inoculates in a natural and open environment, does not need the clean benches, radically simplifies the cultivation ring, and reduces the cultivation cost. Compared with the traditional cultivation, the open cultivation at least reduces over 20% of cost. Because of simple operation, the method can be implemented after preparing the different culture mediums according to prescriptions and adding the anti-bacterial agent. The investment is little, the practicability is strong, and the popularization is good, so the method can be popularized in sugarcane planters.

The invention relates to the technical field of industrialized seedling production and in particular relates to a blueberry tissue culture propagation and ex-vitro rooting method. The method mainly aims to solve the technical problems that rapid industrialized blueberry industrialized seedling production is restrained due to low rooting rate, long rooting period, complex rooting program, high rooting cost and the like during in-vitro blueberry rooting. The method comprises the following steps: selecting and sterilizing an explant, inducing lateral bud germination, performing multiplication culture, and finally performing ex-vitro rooting, wherein in the culture medium for inducing lateral bud germination, the components of the culture medium comprises an improved WPM basic culture medium, 0.8-2mg / L of ZT, 25-35mg / L of white sugar and 5-10g / L of agar; the pH value is regulated to be 5.4; according to the improved WPM basic culture medium, the original potassium sulfate and calcium chloride are replaced by calcium nitrate hydrate and potassium nitrate.

The invention relates to a simple tissue culture method for broussonetia papyrifera. The simple tissue culture method for the broussonetia papyrifera comprises the following steps: preparing a bacteriostatic agent, sterilizing a culture vessel, preparing a culture medium, performing induction culture, performing proliferation culture, performing rooting culture and transplanting a bottled plantlet. By the simple tissue culture method for the broussonetia papyrifera, the culture vessel and the culture medium are not required to be sterilized at high temperature and high pressure, so that the workload and the energy consumption are reduced, the tissue culture link of the broussonetia papyrifera is simplified, and tissue culture cost of the broussonetia papyrifera is lowered; the simple tissue culture method for the broussonetia papyrifera is easy to operate, high in practicality and good in popularization, and the culture medium is prepared according to different formulas and the bacteriostatic agent is added; and compared with the conventional tissue culture method for the broussonetia papyrifera, by the simple tissue culture method for the broussonetia papyrifera, the cost can be reduced by more than 10%.

The invention provides a special culture medium for improving the tissue culture propagation speed of haworthia succulent plants and a tissue culture method. The special culture medium is characterized in that the ingredients of the special culture medium include a basic culture medium and low-dosage 2,4-D. The tissue culture method comprises the following steps of 1 explant selection and sterilization, 2 inoculation and aseptic strain establishment and 3 transfer and expanding propagation. The problem of low tissue culture propagation speed of the haworthia succulent plants can be solved, the propagation speed of the haworthia plants is remarkably improved, the cost of the whole tissue culture is reduced, and the marketing speed of products is improved.

The invention belongs to the technical field of plant tissue breeding, and in particular to a method for reducing the aerial root of hydrangea, aiming at solving the problem that the aerial root is generated in the tissue culture process of the hydrangea and improving the survival rate of tissue culture seedling of the hydrangea. The method is realized by the following steps: the tissue culture seedling of the hydrangea is taken as an experimental material and is inoculated in a liquid rooting medium (1 / 2B5+NAA or IBA 0.1-1.0mg / L+sugar 10g / L pH 4-7) of perlite (40-60ml)+25-35ml; after 30 days, no aerial root is generated in the tissue culture seedling, the rooting rate in a culture flask reaches 100%, and the transplanting survival rate reaches 100%. The method not only improves the transplant survival rate and simplifies the operating procedures of transplantation, but also saves the cost of tissue culture by using the perlite to replace agar and recycling the perlite.

The invention relates to the field of plant tissue culture and discloses a tissue culture method for increasing the survival rate of psidium guajava L.. The tissue culture method comprises the following first step of disinfection of a culture room, the second step of selection of explants, the third step of sterilization of the explants, the fourth step of initial induction culture, the fifth step of subculture, and the sixth step of seedling hardening. According to the method, the explants are disinfected with plant treating liquid, and thus the survival rate of the explants in the tissue culture process is increased; meanwhile, a commonly-used MS culture medium is replaced with cassava alcohol waste liquid, and thus the tissue culture cost can be effectively reduced. The tissue culture method can be applied to various explants of psidium guajava L., the surfaces of the explants can be effectively disinfected without disoperation to the explants, and meanwhile the production cost can be effectively reduced.

The invention discloses a tissue culturing method for regenerating somatic cells of an aquatic iris, belonging to the technical field of plant tissue culturing. The tissue culturing method comprises the steps of: (1) preparing a culturing medium; (2) culturing detoxified tissue culture seedlings of the aquatic iris; (3) transplanting and culturing the tissue culture seedlings; and the like. A tissue culture rapid propagation technical system established by using aseptic seedling leaves as embryoid inducing explants is characterized by high propagation coefficient, high speed, complete structure, high regeneration rate, low production cost and the like; compared with the general organ tissue culturing method, the tissue culturing method for regenerating somatic cells of the aquatic iris has the advantages of shortening the whole culturing period to 75 days, increasing the seedling proliferation coefficient by 2.4 times, reaching a culture seedling rooting rate of 100 percent and a transplanting survival rate of 100 percent, being applicable to industrial seedling culture and realizing commercialized production. The method can be popularized and applied to enterprise production of the aquatic iris.

The invention belongs to the technical field of tissue culture of plants, and particularly relates to a regeneration culture medium for blueberry tissue culture as well as a culture method and application. The regeneration culture medium for blueberry tissue culture comprises an initial culture medium, an enrichment culture medium and a leaf adventitious bud induction culture medium, wherein the initial culture medium is prepared from an improved WPM culture medium, 6-BA, saccharose and plant gel, and the pH is 5.2 to 5.4; the enrichment culture medium is prepared from an improved WPM culture medium, ZT, saccharose and plant gel, and the pH is 5.2 to 5.4; the leaf adventitious bud induction culture medium is prepared from an improved WPM culture medium, TDZ, saccharose and plant gel, and the pH is 5.2 to 5.4; and the improved WPM culture medium is obtained by replacing CaCl2.2H2O in a WPM culture medium with sugar alcohol calcium. The regeneration culture medium has the advantages of strong pertinence, high applicability and low cost, and can effectively increase the inductivity of a callus and the differentiation rate of adventitious buds.

The invention relates to the technical filed of industrialized seedling production, in particular to a method for tissue culture and propagation expansion of iris. The technical problems of slow propagation expansion, long rooting period, complicated rooting procedure, high production cost and the like which restrict rapid industrialized seedling production of the iris are solved. The method comprises the steps that first an explant is selected and disinfected, then tender shoots are induced to germinate, then proliferation culture and rooting culture are performed, and finally domestication and transplanting are performed. The medium which induces tender shoot germination is prepared form the components: modified MS basic medium, 1-2mg / L of 6-BA, 0.3-0.5mg / L of IBA, 25-35mg / L of sugar and5g / L of agar, pH is adjusted to 5.8, and the modified WPM basic culture replaces original potassium nitrate and calcium chloride with calcium nitrate hydrate and potassium sulfate; and the root dripping treatment is carried out again by domestication and transplanting.

The invention discloses an anoectochilus formosanus sound seedling culture method. According to the anoectochilus formosanus sound seedling culture method, anoectochilus formosanus seedlings obtainedby tissue culture are transferred into a sound seedling culture medium and are subjected to sound seedling culture for 90 to 100 days; the sound seedling culture medium contains potatoes and waste anoectochilus formosanu leaves and / or stems. By adopting the anoectochilus formosanus sound seedling culture method, the effect of invigorating anoectochilus formosanus seedlings soundings can be remarkably improved and the medical property of anoectochilus formosanus also can be improved; circulating tissue culture is realized and the cultivation cost is reduced.

The invention relates to a sealwort sterile rooting breeding method in a test tube. The method comprises the following steps: (1) sealwort seed bud selection and sterilization; (2) callus induction; (3) subculture proliferation culture; (4) seedling hardening and rooting; (5) sand bed provisional planting. The sealwort sterile rooting breeding method has the beneficial effects that the sealwort seedling culture period is short; a great number of sealwort seedlings can be formed in five months; the tissue culture cost can be greatly reduced by using multiple shoot proliferation and differentiation; the culture period is shortened; the tissue culture sealwort seedling growth condition is regular and uniform; the medicine effect quality is unified; the breeding speed is high; the influence byexternal environmental factors is avoided; the production can be performed in four seasons in one year; the sealwort tissue culture seedlings have uniform properties; the excellent properties of parents and the growth period consistency can be maintained; the purity of the sealwort tissue culture seedlings is higher; the variation is little; the sealwort field standardized production is facilitated.

The invention relates to an open tissue culture method of polygonatum cyrtonema. According to the method, under the action of the bacteriostatic action of a YJ104 bacteriostatic agent and by addition of the YJ104 bacteriostatic agent into a polygonatum cyrtonema tissue culture medium, the growth and propagation of fungi and bacteria in the polygonatum cyrtonema tissue culture process are inhibited, so that the proliferation and rooting culture of the polygonatum cyrtonema under the bacterial condition are realized. The method is simple to operate, time-saving and labor-saving, greatly reduces the cost, and lays an important foundation for the development of an industrialized seedling production technology of the polygonatum cyrtonema.

The invention provides a rapid propagation technology for aseptic seedlings of curcuma wenyujin. The rapid propagation technology comprises the following steps: (1) explants are obtained; (2) the explants are sterilized; (3) cluster buds are induced into tissue culture seedlings; (4) root tips are induced for producing calluses; (5) the calluses are induced into the aseptic seedlings of the curcuma wenyujin; and (6) the aseptic seedlings of the curcuma wenyujin propagate. The invention adopts the mean of tissue culture seedling for inducing root tip meristem to produce the calluses and obtainsthe aseptic seedlings after differentiation , thereby effectively solving the difficult problem that aseptic meristem is difficult to get directly from the explants, ensuring the obtainment of the aseptic seedlings and solving the problem that the tissue culture seedlings produced through the induction to the explants cannot continuously propagate due to carried endophyte. The technology providedby the invention realizes the continuous propagation of the aseptic seedlings in a MS culture medium added with 2.0 mg / L of 6-benzylaminopurine(6-BA) and 0.5 mg / L of naphthlcetic acid(NAA) and fulfills propagation and rooting synchronously, thereby omitting the processes of rooting and seedling development and reducing the cost in tissue culture.

The invention discloses a tissue culture and rapid propagation method of Jin-mei yangtao actinidia fruits. The method includes the steps: (1) aseptic seedling acquisition; (2) adventitious bud regeneration; (3) adventitious bud proliferation and strong seedling culture; (4) rooting culture; (5) transplanting. The method is characterized by a germination culture medium, a regeneration culture medium, a proliferated strong seedling culture medium, a rooting culture medium and corresponding culture conditions. The four targeted culture media are used for tissue culture of the Jin-mei yangtao actinidia fruits, a large number of Jin-mei yangtao actinidia fruit seeds and seedlings can be rapidly propagated in a short time, seed and seedling quality can be maximally ensured by tissue culture, seed and seedling propagation speed is increased, industrial production of the seeds and seedlings is realized, and the requirements of production for the Jin-mei yangtao actinidia fruit seeds and seedlings are met. Variety seeds and seedlings are directly subjected to tissue culture, a variety cutting orchard is built, the bottleneck of scion grafting in traditional grafting propagation is broken, and production requirements are sufficiently met.

The invention discloses a culture medium for tissue culture of valeriana jatamansi and a culture method. The culture medium for the tissue culture has the advantages of simple components, low cost andeasiness for obtaining; the induction rate, multiplication coefficient and rooting rate of the valeriana jatamansi can be remarkably improved; the tissue culture efficiency can be effectively improved and the production method is reduced; the culture medium has market-oriented application value.

The invention relates to an antibacterial culture method for genetically-transformed tobacco. The antibacterial culture method comprises the steps of: bacteriostat preparation; culture vessel sterilization; agrobacterium tumefacien transformation; culture medium preparation; co-culture; screening culture; rooting culture; resistive seedling detection. Due to the adoption of the antibacterial culture method for the genetically-transformed tobacco, provided by the invention, a culture vessel and a culture medium are both not needed to be sterilized at high temperature and high pressure, so that the workload and energy consumption are reduced, the culture link of the genetically-transformed tobacco is simplified, and the culture cost of the genetically-transformed tobacco is reduced; the antibacterial culture method for the genetically-transformed tobacco, provided by the invention, is simple in operation, strong in practicability and good in popularization, and only a bacteriostat is added after culture mediums are prepared according to different formulas; in addition, compared with the conventional method, the antibacterial culture method for the genetically-transformed tobacco can be used for reducing the cost by more than 10%.

The invention discloses a bacteriostatic agent for an open type bacteriostatic culture medium, the open type bacteriostatic culture medium and a preparation method of the open type bacteriostatic culture medium. The bacteriostatic agent for the open type bacteriostatic culture medium comprises 0.1-0.3 g / L of cefixime, 0.1-0.3 g / L of streptomycin, 0.1-0.3 g / L of carbendazim and 0.1-0.3 g / L of mancozeb, wherein the concentration refers to the final concentration during use. The open type bacteriostatic culture medium comprises the bacteriostatic agent and also comprises a basic culture medium and a curing agent. The bacteriostatic agent has a good bacteriostatic effect, the whole operation process is simpler, the tissue culture cost is reduced, indoor operation can be allowed, and the accessthreshold is low. The operation is extensive, promotion in rural areas is facilitated, and mass production is facilitated.

The invention discloses a tissue culture inoculating method for dendrobium officinale. The tissue culture inoculating method for the dendrobium officinale comprises the following steps: selection of dendrobium officinale pods, sowing of seed embryos, preparation of sterile seed embryo mixed liquid, preparation of a germinating and rooting culture medium, and inoculation and culture of the seed embryos. The method disclosed by the invention is a simple and effective tissue culture inoculating method. According to the method, the germinating and rooting culture medium is inoculated to the cultured seed embryos through once induction, so that repeated culture rotation is not required; moreover, the germinating and rooting culture medium prepared in the method disclosed by the invention is mainly based on a natural antibacterial additive, and does not have harmful chemical component additive, so that the problems that the debugging time is long, the workload is long, the pollution rate is high, and the stability of produced seed seedlings is poor in conventional artificial one-by-one inoculation can be effectively solved; the pollution rate in the tissue culture process of the dendrobium officinale can be greatly reduced, and the tissue culture cost of the dendrobium officinale can be reduced; the tissue culture inoculating efficiency and the transplanting survival rate of the dendrobium officinale are greatly improved; the production efficiency is 3 to 4 times that of the conventional method; the transplanting survival rate can reach more than or equal to 92 to 93 percent.

The invention discloses a tissue culturing method for regenerating somatic cells of an aquatic iris, belonging to the technical field of plant tissue culturing. The tissue culturing method comprises the steps of: (1) preparing a culturing medium; (2) culturing detoxified tissue culture seedlings of the aquatic iris; (3) transplanting and culturing the tissue culture seedlings; and the like. A tissue culture rapid propagation technical system established by using aseptic seedling leaves as embryoid inducing explants is characterized by high propagation coefficient, high speed, complete structure, high regeneration rate, low production cost and the like; compared with the general organ tissue culturing method, the tissue culturing method for regenerating somatic cells of the aquatic iris has the advantages of shortening the whole culturing period to 75 days, increasing the seedling proliferation coefficient by 2.4 times, reaching a culture seedling rooting rate of 100 percent and a transplanting survival rate of 100 percent, being applicable to industrial seedling culture and realizing commercialized production. The method can be popularized and applied to enterprise production of the aquatic iris.

The invention discloses a culture method for prolonging the storage period of kiwi fruit tissue culture seedlings. A certain amount of sucrose is added on the basis of the conventional subculture medium of kiwi fruit tissue culture seedlings, so that the storage time of the tissue culture seedlings can be effectively prolonged. The problem of short storage period of the existing kiwi fruit tissueculture seedlings is solved. Through the storage of the kiwi fruit tissue culture seedlings performed according to the method provided by the invention, the growth of the tissue culture seedlings canbe generally delayed for 4 to 5 months; the survival rate of the tissue culture seedlings can reach 80 percent or higher.

The invention discloses a method for in vitro cultivation and regeneration of curculigo grass leaves, comprising the steps of selecting explants, cleaning and disinfecting the explants, inducing clustered buds, proliferating clustered buds, and rooting. The present invention successfully establishes a low-cost tissue culture and rapid propagation system of curculigo, which lays a technical foundation for realizing the protection, artificial cultivation, seedling cultivation and establishment of gene banks of curculigo wild resources, and solves the problem of over-exploitation of curculigo wild resources, The supply of medicinal materials is insufficient, the planting is not in scale, the planting is limited by the supply of seedlings, and the quality of medicinal materials is unstable.

The invention discloses an opening sealing method of a glass culture vessel for callus culture. The opening of the glass culture vessel of 60-90mm is sealed by a preservative film and the operation steps are as follows: (a) cutting a preservative film roll into annular circles in 2-3cm width with a scalpel vertical to the length direction of the preservative film roll and placing the annular circles on a clean bench to disinfect and sterilize; and (b) taking the glass culture vessel of 60-90mm for callus culture and sealing the cover opening of the glass culture vessel by the common preservative film which is disinfected and sterilized in the step (a), and in sealing, tightly attaching the preservative film to the cover opening at the bottom of the glass culture vessel, rotating the preservative film around the culture vessel for two circles and then slightly pulling apart the preservative film, thus finishing opening sealing. The opening sealing method of the glass culture vessel for callus culture is convenient to operate and in low material cost, can easily obtain materials, effectively lower the browning rate and the pollution rate of the callus and facilitate observing the tissue culture results and improving the recyclability rate of the culture vessel.

The invention relates to a method for quickly obtaining transgenic eggplant plants and belongs to the technical field of a transgenic eggplant plant obtaining method. In order to solve the problem of low eggplant genetic transformation efficiency, the invention provides the method for quickly obtaining the transgenic eggplant plants, which comprises the following steps: firstly, pre-culturing eggplant explants on a regenerative culture medium, activating agrobacterium tumefaciens carrying cryIIIA genes, infecting the eggplant explants with the agrobacterium tumefaciens and culturing the infected eggplant explants on the regenerative culture medium to transform explant cells with the agrobacterium tumefaciens; then transferring the explants onto a selective culture medium for further culture so as to form adventitious buds and further form the complete plants. The method provided by the invention has the benefits that both the regenerative culture medium and the selective medium contain thiabendron and N6-[isoamene]-adenine, so that the induction efficiency is high, the quantity of the adventitious buds formed by the explants can be increased, and the regeneration efficiency growth rate can reach 163 percent; according to the method provided by the invention, the transformation efficiency of transgenic eggplant breeding is improved, and the transformation period is shortened.

The invention discloses a medium for inhibiting endophyte and material browning in date palm tissue culture, comprising reagent A, reagent B and reagent C; the reagent A is 1 / 2MS containing Shannong No. 1 type I fungicide Salt solution; Described reagent B contains Shannong No. 1 type III bactericide, phytogel, sucrose, glutamine, pyridoxine hydrochloride, nicotinic acid, 6-benzylaminoadenine, isopentenyl adenine, 2 , the MS salt solution of 4-dichlorophenoxyacetic acid, thidiazuron, naphthalene acetic acid, polyvinylpyrrolidone, ethanesulfonic acid monohydrate and activated carbon; Described reagent C is a vegetal gel, sucrose, glutamine, hydrochloric acid of pyridoxine, niacin, inositol, 6-benzylaminoadenine, isopentenyladenine, 2,4-dichlorophenoxyacetic acid, naphthaleneacetic acid, polyvinylpyrrolidone, ethanesulfonic acid monohydrate and activated carbon MS salt solution. The invention can inhibit the growth of endophytes during the culturing process, control the contamination rate of endophytes below 9%, and control the browning rate of explants below 13%.