A kind of intermittent immersion type orchid plant protocorm multiplication and rapid propagation method

A technology of plants and protocorms, which is applied in the field of plant tissue culture, can solve problems such as the introduction and improvement of intermittent immersion culture devices, and the influence of explant proliferation efficiency, so as to improve the quality of cultures and reproduction coefficients, and high utilization of culture space , Possibility of contamination is small

Active Publication Date: 2017-09-26
INST OF BOTANY CHINESE ACAD OF SCI
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Problems solved by technology

The patent No. CN 101768566A discloses a batch-type fermentation culture method of Dendrobium officinale, but both studies have not introduced and improved the most important intermittent immersion culture device, and the size and shape of the device and the placement of plant materials are different. have a significant impact on the proliferation efficiency of explants

Method used

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  • A kind of intermittent immersion type orchid plant protocorm multiplication and rapid propagation method
  • A kind of intermittent immersion type orchid plant protocorm multiplication and rapid propagation method

Examples

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Embodiment 1

[0036] Example 1 Orchid PLB propagation and rapid propagation culture

[0037] 1. Take orchid plants stem The tips, leaves or stems are rinsed in running water for 2h, soaked in 75% alcohol for 10-20s, sterilized with HgCl2 for 8min, rinsed with sterile water 4 times to obtain sterile PLB induction material.

[0038] 2. Put the explants into the induction medium, about 3-4 weeks, PLB will appear on the explants.

[0039] 3. A plurality of unloaded sieve trays are stacked in the fixed frame, and then the unloaded component is put into the main body of the culture container, and then the cover body is sealed with the main body of the culture through threaded engagement. In addition, the outer opening of the culture container body is sealed with a cotton ball, and the whole device is to be sterilized by high temperature and high pressure;

[0040] 4. The silicone hose can be sealed with newspaper and sterilized by high temperature and high pressure;

[0041] 5. Seal the openings of the ...

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Abstract

The invention discloses an intermittent immersion type orchid protocorm-like propagation method, which comprises the following steps: carrying out aseptic culture of explants to induce orchid protocorm (PLB); 2) the step 1) The obtained PLB is placed in a connecter-type intermittent plant tissue culture device for intermittent immersion culture; the present invention has the advantages of high utilization of the culture space and is conducive to the exchange of gas components in the culture, thereby improving the quality of the culture and the reproduction coefficient, suitable for large Large-scale production; culture equipment and accessories are easy to disinfect and sterilize as a whole, the culture process is fully enclosed, and the chance of contamination is small; the structure is simple and compact, and energy consumption is low, which saves culture space and reduces tissue culture costs; reduces the overall tissue culture process. The operation of transfer and subculture of seedlings saves labor and production costs.

Description

Technical field [0001] The invention belongs to the technical field of plant tissue culture, and relates to a method for the proliferation and regeneration of orchid protocorm-like bodies (PLB). Background technique [0002] Plant tissue culture technology has the advantages of large and rapid reproduction, easy control of experimental conditions, continuous production and high production efficiency. It is widely used in crop breeding and germplasm resource preservation, as well as detoxified seedlings of flowers, fruit trees, vegetables, forest trees and Chinese herbal medicines Production and rapid reproduction, such as the asexual and rapid reproduction of lily detox seed balls. [0003] Traditional solid or semi-solid tissue culture methods require a large amount of agar, and a large number of containers need to be processed during filling and cleaning, which consumes a lot of labor and leads to high production costs. [0004] At present, some intermittent immersion culture devi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 石雷李杨柴敏
Owner INST OF BOTANY CHINESE ACAD OF SCI
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