A tissue culture method for improving the survival rate of bananas
A technology of tissue culture and survival rate, which is applied in the field of plant tissue culture, can solve problems such as improvement of disinfectants, achieve the effects of promoting growth, reducing tissue culture costs, and promoting plant growth
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Embodiment 1
[0033] Spray lime powder on the ground of the cultivation room with closed doors and windows 20 hours before the cultivation, spray concentration in the air and be 75% medical alcohol, and carry out sterilization; select the banana sucking bud without damage by diseases and insect pests to be the explant, long 2cm, diameter 1cm; Rinse the explants with tap water for 9 minutes, remove them and soak them in 75% alcohol solution for 5 minutes, and soak them for 10 minutes in a plant treatment solution with a mass concentration of 5% after the explants are drained (the composition and weight of the plant treatment solution The percentages are as follows: 37% Ganoderma lucidum extract, 10% Polygonum officinalis juice, 17% garlic juice, 20% onion juice, 8% Huoxiang juice, 8% cactus juice); Inoculate the body in the induction medium and carry out the induction culture in the incubator (the induction medium consists of: COD content is 15% cassava alcohol waste liquid of 300mg / L, 2.0g / L...
Embodiment 2
[0035] 22 hours before the cultivation, spray lime powder on the ground of the cultivation room with doors and windows closed, spray concentration in the air and be 75% medical alcohol, and carry out sterilization; select the banana sucking buds without damage by diseases and insect pests as explants, with a length of 3 cm and a diameter of 2 cm; Rinse the explants with tap water for 12min, remove them and soak them in 75% alcohol solution for 7min, after the explants are drained, soak them in 8% plant treatment solution for 20min (composition and weight of the plant treatment solution) The percentages are as follows: 50% Ganoderma lucidum extract, 15% Polygonum officinalis juice, 10% garlic juice, 10% onion juice, 5% Huoxiang juice, 10% cactus juice); Inoculation of the body was carried out in an incubator in an induction medium for induction cultivation (induction medium consists of: 20% cassava alcohol waste liquid with a COD content of 800mg / L, 4.0g / L sucrose solution, 8.0g...
Embodiment 3
[0037] Spray lime powder on the ground of the cultivation room with doors and windows closed 24 hours before the cultivation, and spray the medical alcohol with a concentration of 75% in the air to carry out sterilization; select the banana sucking buds without damage by diseases and insect pests as explants, with a length of 2.5 cm and a diameter of 1.5 cm. cm; Rinse the explants with tap water for 11 minutes, take them out and soak them in 75% alcohol solution for 6 minutes, and after the explants are drained, soak them for 15 minutes in a plant treatment solution with a mass concentration of 5%-8% (plant treatment The composition and percentage by weight of liquid are as follows: 30% Ganoderma lucidum extract, 20% Polygonum officinalis juice, 20% garlic juice, 15% onion juice, 10% Huoxiang juice, 5% cactus juice); The inoculation of the explants after the bacteria is carried out induction culture in the incubator in induction medium (induction medium consists of: COD content...
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