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Opening sealing method of glass culture vessel for callus culture

A callus, petri dish technology, applied in horticultural methods, botanical equipment and methods, applications, etc., can solve problems such as inconvenience, and achieve the effects of reducing the pollution rate, reducing the browning rate, and having a neat and clean appearance.

Inactive Publication Date: 2011-10-19
GANSU AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The inconvenience of this sealing method is particularly prominent in large-scale callus culture

Method used

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  • Opening sealing method of glass culture vessel for callus culture

Examples

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Effect test

Embodiment 1

[0025] A method for sealing a petri dish used during callus culture. A 90mm glass petri dish is sealed with a preservative film. The operation steps are as follows:

[0026] (a) Use a scalpel to cut the common plastic wrap roll perpendicular to its length into an annular ring with a width of 3 cm, and place it on an ultra-clean workbench for sterilization;

[0027] (b) Take the 90mm glass petri dish used for callus culture, seal the opening with the ordinary plastic wrap after sterilization in step (a), when sealing, press the plastic wrap tightly against the cover that needs to be sealed at the bottom of the glass petri dish After rotating the petri dish for 2 turns, lightly pull the plastic wrap to tear it off to complete the sealing of the petri dish.

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Abstract

The invention discloses an opening sealing method of a glass culture vessel for callus culture. The opening of the glass culture vessel of 60-90mm is sealed by a preservative film and the operation steps are as follows: (a) cutting a preservative film roll into annular circles in 2-3cm width with a scalpel vertical to the length direction of the preservative film roll and placing the annular circles on a clean bench to disinfect and sterilize; and (b) taking the glass culture vessel of 60-90mm for callus culture and sealing the cover opening of the glass culture vessel by the common preservative film which is disinfected and sterilized in the step (a), and in sealing, tightly attaching the preservative film to the cover opening at the bottom of the glass culture vessel, rotating the preservative film around the culture vessel for two circles and then slightly pulling apart the preservative film, thus finishing opening sealing. The opening sealing method of the glass culture vessel for callus culture is convenient to operate and in low material cost, can easily obtain materials, effectively lower the browning rate and the pollution rate of the callus and facilitate observing the tissue culture results and improving the recyclability rate of the culture vessel.

Description

technical field [0001] The invention relates to a sealing method for a glass culture dish, in particular to a sealing method for a 60mm-90mm glass culture dish used for callus culture. Background technique [0002] Plant tissue culture technology refers to the cultivation of isolated plant organs, tissues, cells and protoplasts into complete plants in an artificially prepared environment under sterile conditions. Using tissue culture, not only can mass-produce excellent clones, obtain a variety of metabolites needed by humans, but also obtain haploid, triploid, polyploid and aneuploid, which is also the development of transgenic technology and cell fusion technology. Base. In the process of using glass culture dishes for callus culture, the sealing of glass culture dishes is one of the key factors affecting the good growth of callus and the reduction of pollution rate. The sealing method currently used—— parafilm Although the sealing film (glue) sealing method can reduce ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 师尚礼陶茸李玉珠曹文侠赵桂琴祁娟白小明
Owner GANSU AGRI UNIV
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