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6802 results about "Culture tissue" patented technology

Tissue culture is the growth of tissues or cells separate from the organism. This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar.

Apparatus and method for bioelectric stimulation, healing acceleration, pain relief, or pathogen devitalization

An method and method for generating an electrical signal for use in biomedical applications, including two timing-interval generators, each optionally driving a multistep sequencer; analog, digital or hybrid means for combining the resulting timed signals into a complex electrical signal; optional filtering means for blocking direct current, removing selected frequency components from the resulting signal, and / or providing voltage step-up if needed; and conductive means for coupling the resulting signal to a human or animal body, food, beverage or other liquid, cell or tissue culture, or pharmaceutical material, in order to relieve pain, stimulate healing or growth, enhance the production of specific biochemicals, or devitalize selected types of organisms.
Owner:HEALTHONICS INC

Plants and seeds of corn variety I351729

According to the invention, there is provided seed and plants of the corn variety designated I351729. The invention thus relates to the plants, seeds and tissue cultures of the variety I351729, and to methods for producing a corn plant produced by crossing a corn plant of variety I351729 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety I351729 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety I351729.
Owner:MONSANTO TECH LLC

Nanofibrillar structure and applications including cell and tissue culture

A nanofibrillar structure for cell culture and tissue engineering is disclosed. The nanofibrillar structure can be used in a variety of applications including methods for proliferating and / or differentiating cells and manufacturing a tissue. Also disclosed is an improved nanofiber comprising a lipid, lipophilic molecule, or chemically modified surface. The nanofibers can be used in a variety of applications including the formation of nanofibrillar structures for cell culture and tissue engineering.
Owner:BOARD OF TRUSTEES OPERATING MICHIGAN STATE UNIV

Isolation, cultivation and uses of stem/progenitor cells

The present invention relates to a method for isolating stem / progenitor cells from the amniotic membrane of umbilical cord, wherein the method comprises separating the amniotic membrane from the other components of the umbilical cord in vitro, culturing the amniotic membrane tissue under conditions allowing cell proliferation, and isolating the stem / progenitor cells from the tissue cultures. The isolated stem cell cells can have embryonic stem cell-like properties and can be used for various therapeutic purposes. In one embodiment, the invention relates to the isolation and cultivation of stem cells such as epithelial and / or mesenchymal stem / progenitor cells under conditions allowing the cells to undergo mitotic expansion. Furthermore, the invention is directed to a method for the differentiation of the isolated stem / progenitor cells into epithelial and / or mesenchymal cells.
Owner:CELLRESEARCH CORP PTE LTD

Plants and seeds of corn variety cv847814

According to the invention, there is provided seed and plants of the corn variety designated CV847814. The invention thus relates to the plants, seeds and tissue cultures of the variety CV847814, and to methods for producing a corn plant produced by crossing a corn plant of variety CV847814 with itself or with another corn plant, such as a plant of another variety. The invention further relates to corn seeds and plants produced by crossing plants of variety CV847814 with plants of another variety, such as another inbred line. The invention further relates to the inbred and hybrid genetic complements of plants of variety CV847814.
Owner:MONSANTO TECH LLC

Tissue engineered tendons and ligaments

Connective tissue, including neo-tendons and ligaments, has been constructed using biodegradable synthetic scaffolds seeded with tenocytes. The scaffolds are preferably formed from biodegradable fibers formed of a polymer such as polyglycolic acid-polylactic acid copolymers, and seeded with cells isolated from autologous tendon or ligament by means of enzymatic digestion or direct seeding into tissue culture dishes from explants. The cell polymer constructs are then surgically transplanted to replace missing segments of functioning tendon or ligament.
Owner:MASSACHUSETTS INST OF TECH +1

Plants and seeds of corn variety I325350

According to the invention, there is provided seed and plants of the corn variety designated I325350. This invention thus relates to the plants, seeds and tissue cultures of the variety I325350, and to methods for producing a corn plant produced by crossing a corn plant of variety I325350 with itself or with another corn plant, such as a plant of another variety. This invention further relates to corn seeds and plants produced by crossing plants of variety I325350 with plants of another variety, such as another inbred line, and to crosses with related species. This invention further relates to the inbred and hybrid genetic complements of plants of variety I325350, and also to the SSR and isozyme typing profiles of corn variety I325350.
Owner:MONSANTO TECH LLC

Plants and seeds of hybrid corn variety CH093858

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH093858. The invention thus relates to the plants, seeds and tissue cultures of the variety CH093858, and to methods for producing a corn plant produced by crossing a corn plant of variety CH093858 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH093858.
Owner:MONSANTO TECH LLC

Bioreactor for growing cell or tissue cultures

The invention relates to a bioreactor comprising a chamber for containing cells or tissue cultures within a culture medium. The bioreactor also comprises a detector capable of detecting a change in one or more metabolites associated with growth of the cell or tissue cultures within the chamber and a chamber drive capable of rotating the chamber at a first speed about a first axis and a second speed about a second axis, the second axis being disposed at an angle relative to the first axis. In use, the magnitude of the first speed and the second speed are independently variable of each other.
Owner:SINGAPORE POLYTECHNIC +1

Plants and seeds of hybrid corn variety CH956311

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH956311. The invention thus relates to the plants, seeds and tissue cultures of the variety CH956311, and to methods for producing a corn plant produced by crossing a corn plant of variety CH956311 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH956311.
Owner:MONSANTO TECH LLC

Bio-macromolecular hydrogel and preparation method thereof

The invention discloses a bio-macromolecular hydrogel prepared by enzyme catalysis and ion cross-linking, and a preparation method of the bio-macromolecular hydrogel. The bio-macromolecular hydrogel comprises a protein or polypeptide bio-macromolecular network formed by enzyme-catalyzed cross-linking of protein or polypeptide or amino acid residue-containing molecule, accounting for 1-99 wt% of total dry mass of the hydrogel; and a polysaccharide bio-macromolecular network formed by bivalent ion cross-linking of polysaccharide macromolecule, accounting for 1-99 wt% of total dry mass of the hydrogel. The above two networks are inter-penetrated without chemical bonding. The hydrogel has the advantages of excellent mechanical properties, no use of chemical cross-linking agent, simple and effective preparation method, good bio-compatibility and mechanical strength, and capability of steam sterilization; may be in the forms of wet or dry film, porous sponge, tube and particles; and can be used in cell / tissue culture, and used as tissue repair material, tissue engineered scaffold or drug release carrier.
Owner:SOUTHEAST UNIV

Lung and lung cancer tissue culture method and method using lung and lung cancer tissue culture method to build lung cancer mouse animal model

ActiveCN106967672AGenetic stabilityGenetic uniformityCell dissociation methodsArtificial cell constructsImmunofluorescent stainLung tissue
The invention discloses a method for culturing normal human lung tissue and a lung cancer tissue organoid in an in-vitro manner. The method includes: acquiring fresh human-derived lung tissue cells, and performing collagen digestion on the fresh human-derived lung tissue cells to obtain single cells; culturing human lung tissue and the lung cancer tissue organoid under in-vitro 3D culture conditions; performing H&E staining to determining the structure and form, and using q-PCR to detect related gene expression; using immunofluorescent staining to authenticate cell sources and detect related protein expression. The invention further discloses a method for building a mouse animal model based on the organoid. The method for culturing the normal human lung tissue and the lung cancer tissue organoid and the method for building the mouse animal model have the advantages that the methods are significant to the building of large-scale and good-consistency human-derived in-situ lung cancer animal models, and a good basis and related application prospect are provided for the fundamental researches of lung cancer.
Owner:WEST CHINA HOSPITAL SICHUAN UNIV

Plants and seeds of hybrid corn variety CH779362

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH779362. The invention thus relates to the plants, seeds and tissue cultures of the variety CH779362, and to methods for producing a corn plant produced by crossing a corn plant of variety CH779362 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH779362.
Owner:MONSANTO TECH LLC

Cell lines for expressing enzyme useful in the preparation of amidated products

InactiveUS20060292672A1High expressionGood for survivalAnimal cellsSugar derivativesPeptidylglycine alpha-amidating monooxygenaseChemical compound
Cell lines are provided for expressing peptidylglycine alpha-amidating monooxygenase (PAM), or one of its two catalytic domains. High levels of enzyme expression are achieved while utilizing a non-animal source, low protein tissue culture medium. A robust two-step downstream purification results in high enzyme purity. Resulting PAM, or its PHM catalytic domain, is used to catalyze the enzymatic conversion of X-Gly to X-alpha-hydroxy-Gly or X—NH2 (X being a peptide or any chemical compound having a carbonyl group to which a glycine group can be covalently attached). Methods of preparing preferred cell lines are also set forth.
Owner:ENTERIS BIOPHARMA
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