The invention provides a functional
molecular marker for rice anti-blast
gene Pi9 and application thereof, belonging to the field of
crop molecular genetic breeding science. The invention finds out that the rice anti-blast
gene Pi9 has a 10bp
insertion / deletion site positioned between the
gene promoter -516 and -517, wherein the
nucleotide sequence is disclosed as SEQ ID NO.1; and on such basis, the invention develops a method of the functional
molecular marker for gene Pi9. By detecting the functional
molecular marker, the invention can accurately detect whether genomes of different rice species contain the Pi9 functional gene and detect the homozygotic state, and can be used for screening rice
hybrid transformed descendant plants to enhance the breeding efficiency of the rice anti-blast material, thereby effectively controlling the size of the breeding
population, obviously saving the breeding and screening cost and obtaining the anti-blast rice species containing the Pi9 functional gene.