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Dry-powder-type culturing substrate and preparing method

A technology of culture medium and dry powder is applied in the field of plant cloning and preparation of culture medium, which can solve the problems of tediousness and trouble in configuring culture medium, and achieve the effect of easy manufacture.

Inactive Publication Date: 2007-11-14
陈曦
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the troublesome and cumbersome fundamental disadvantages of the existing culture medium configuration, the present invention mainly provides a dry powder culture medium and its preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] In order to realize the dry powder culture medium of the present invention, the present invention provides following technical scheme:

[0011] Now take the preparation of a standard medium as an example, the following table shows the formula and classification for preparing a liter of medium:

[0012] Mass element classes:

potassium nitrate

1.9g

ammonium nitrate

1.65g

Calcium Chloride Dihydrate

0.44g

Magnesium Sulfate Heptahydrate

0.37g

Potassium dihydrogen phosphate

0.17g

Trace elements and iron salts:

Disodium edetate

37.3mg

Ferrous Sulfate Heptahydrate

27.8mg

Manganese Sulfate Tetrahydrate

22.3mg

Zinc sulfate heptahydrate

8.6mg

boric acid

6.2mg

potassium iodide

0.83mg

Sodium molybdate dihydrate

0.25mg

Copper Sulfate Pentahydrate

0.025mg

Cobalt chloride hexahydrate

0.025mg

Vitamins...

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PUM

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Abstract

Powder type culture medium is from plenty of element kinds , trace element and iron salt and vitamin kind of composition, after processing manufacture the original powder that makes powder culture medium. The various components of the medium and the final product were dry powder. The preparation Method: First, concentrate all plenty of elements to collect reserve; Mix trace element and vitamin kind totally to wrap to attach , and then mix the thin medicine and plenty of elements of trace element totally, under the role with centrifugal high speed, making it even scatter and wrap up to glue to unite. So, make the original powder of powder culture medium for manufacture. This medium-dry powder manufacturing easier, does not require the use of complex machines and equipment, and low-cost easy to promote. The product is of high quality in existing product. It can be used in plant breeding, tissue culture and molecular biology research, and can be used for various commercial purposes.

Description

technical field [0001] The invention relates to the field of biotechnology, and specifically relates to a preparation method and a use method of a culture medium for applying plant cloning technology to scientific research and large-scale plant tissue culture production. The products and methods are of benefit to plant breeders, tissue culture and molecular biology researchers and can be used in a variety of commercial applications. Background technique [0002] For the normal growth and development of tissue culture plants, a medium is required. The main components of the existing plant cloning medium include a large number of elements (such as potassium nitrate, ammonium nitrate, calcium chloride, etc.), trace elements, iron salts (ferrous sulfate, copper sulfate, manganese sulfate, etc.), vitamins (VB1, VB6, etc.), coagulant (agar, cold fat or Zhentai gum) and sucrose. [0003] The traditional configuration method is to boil the coagulant with water, add water-soluble m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 陈曦
Owner 陈曦
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