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A medium and application for inhibiting endophyte and material browning in date palm tissue culture

A technology of endophytic bacteria and date palms, which is applied in the field of plant biology to achieve the effects of improving utilization, shortening the culture period, and reducing the cost of tissue culture

Active Publication Date: 2022-07-15
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there is no use and record of this technology in the field of date palm tissue culture both at home and abroad at present.

Method used

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  • A medium and application for inhibiting endophyte and material browning in date palm tissue culture
  • A medium and application for inhibiting endophyte and material browning in date palm tissue culture
  • A medium and application for inhibiting endophyte and material browning in date palm tissue culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Medium for inhibiting endophyte and material browning in date palm tissue culture, including reagent A, reagent B and reagent C;

[0065] The reagent A is a 1 / 2 MS salt solution containing Shannong No. 1 type I fungicide (final concentration 1% wt);

[0066]The reagent B contains Shannong No. 1 type III fungicide (final concentration 0.1%wt), phytogel (final concentration 3.5g / L), sucrose (final concentration 30g / L), glutamine (final concentration 100mg / L) L), pyridoxine hydrochloride (final concentration 1mg / L), niacin (final concentration 0.5mg / L), 6-benzylaminoadenine (final concentration 0.2mg / L), isopentenyl adenine (final concentration 0.1mg / L), 2,4-dichlorophenoxyacetic acid (final concentration 0.02mg / L), thidiazuron (final concentration 0.02mg / L), naphthalene acetic acid (final concentration 0.08mg / L), polyvinylpyrrolidone MS salt solution of (final concentration 0.5g / L), ethanesulfonic acid monohydrate (final concentration 1g / L) and activated carbon (final co...

Embodiment 2

[0082] Medium for inhibiting endophyte and material browning in date palm tissue culture, including reagent A, reagent B and reagent C;

[0083] The reagent A is a 1 / 2 MS salt solution containing Shannong No. 1 type I fungicide (final concentration 4.5% wt);

[0084] The reagent B contains Shannong No. 1 type III fungicide (final concentration 0.12%wt), phytogel (final concentration 3.5g / L), sucrose (final concentration 30g / L), glutamine (final concentration 100mg / L) L), pyridoxine hydrochloride (final concentration 1mg / L), niacin (final concentration 0.5mg / L), 6-benzylaminoadenine (final concentration 0.5mg / L), isopentenyl adenine (final concentration 0.2mg / L), 2,4-dichlorophenoxyacetic acid (final concentration 0.05mg / L), thidiazuron (final concentration 0.05mg / L), naphthalene acetic acid (final concentration 0.1mg / L), polyvinylpyrrolidone MS salt solution of (final concentration 0.5g / L), ethanesulfonic acid monohydrate (final concentration 1g / L) and activated carbon (final...

Embodiment 3

[0100] Medium for inhibiting endophyte and material browning in date palm tissue culture, including reagent A, reagent B and reagent C;

[0101] The reagent A is a 1 / 2 MS salt solution containing Shannong No. 1 type I fungicide (final concentration 5% wt);

[0102]The reagent B contains Shannong No. 1 type III fungicide (final concentration 0.2%wt), phytogel (final concentration 3.5g / L), sucrose (final concentration 30g / L), glutamine (final concentration 100mg / L) L), pyridoxine hydrochloride (final concentration 1mg / L), niacin (final concentration 0.5mg / L), 6-benzylaminoadenine (final concentration 0.8mg / L), isopentenyl adenine (final concentration 0.5mg / L), 2,4-dichlorophenoxyacetic acid (final concentration 0.08mg / L), thidiazuron (final concentration 0.08mg / L), naphthalene acetic acid (final concentration 0.12mg / L), polyvinylpyrrolidone MS salt solution of (final concentration 1g / L), ethanesulfonic acid monohydrate (final concentration 1g / L) and activated carbon (final conc...

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PUM

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Abstract

The invention discloses a medium for inhibiting endophyte and material browning in date palm tissue culture, comprising reagent A, reagent B and reagent C; the reagent A is 1 / 2MS containing Shannong No. 1 type I fungicide Salt solution; Described reagent B contains Shannong No. 1 type III bactericide, phytogel, sucrose, glutamine, pyridoxine hydrochloride, nicotinic acid, 6-benzylaminoadenine, isopentenyl adenine, 2 , the MS salt solution of 4-dichlorophenoxyacetic acid, thidiazuron, naphthalene acetic acid, polyvinylpyrrolidone, ethanesulfonic acid monohydrate and activated carbon; Described reagent C is a vegetal gel, sucrose, glutamine, hydrochloric acid of pyridoxine, niacin, inositol, 6-benzylaminoadenine, isopentenyladenine, 2,4-dichlorophenoxyacetic acid, naphthaleneacetic acid, polyvinylpyrrolidone, ethanesulfonic acid monohydrate and activated carbon MS salt solution. The invention can inhibit the growth of endophytes during the culturing process, control the contamination rate of endophytes below 9%, and control the browning rate of explants below 13%.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a medium for inhibiting the browning of endophytes and materials in the process of tissue culture of date palm, a preparation method and a use method. Background technique [0002] Date palm is a perennial tropical palm plant, dioecious, and is an important woody food crop in Arab countries. Date palm can use seeds to reproduce offspring, but because it is a dioecious plant and is in a highly heterozygous state, this method of reproduction will lose the target traits of the parent in production, and cannot guarantee the excellent characteristics and fruit of the mother plant. feature. [0003] Currently internationally, branching and tissue culture of tiller seedlings are the main methods for the propagation of date palm seedlings. During the growth cycle of each date palm seedling, the reproduction number of tiller seedlings is 15-25, and the reproduction number is severely l...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/002A01H4/005
Inventor 徐中亮张宁王义符海泉王富有
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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