319 results about "Naphthaleneacetic Acids" patented technology

The invention discloses a plant growth regulating composition and its application. The composition comprises the following components: A) natural abscisic acid, B) gibberellic acid (GA3, or of GA4 +7), and C) an existing plant growth regulator. The existing plant growth regulator is selected from one or more than two of 6-BA (6-benzylaminopurine), thidiazuron, brassinolide, sodium nitrophenolate, potassium nitrophenolate, chlormequat chloride, triacontanol, naphthlcetic acid and its salt, indolebutyric acid and its salt, indoleacetic acid and its salt, ethephon, diethyl aminoethyl hexanoate, paclobutrazol, uniconazole, fulvic acid, Mepiquat chloride, nitrohumic acid, jasmonic acid and triapenthenol. Specifically, the components A), B) and C) are in a weight ratio of 1:0.001-500:0.001-500. The S-ABA (S-abscisic acid) added in the invention can expand the prevention and treatment objects, reduce or prevent phytotoxicity, reduce dosage, and lower the use cost.

The invention relates to a plant nutrient liquid fertilizer. In order to solve the problem of over fertilization, the plant nutrient liquid fertilizer is prepared from the following raw materials in proportion by weight: aqua brassin 12-36, gibberellin 1-4, indoleacetic acid 30-80, abscisic acid 10-20, naphthaleneacetic acid 50-90, salicylic acid 80-180, vitamin C 1-3, cane sugar 40-100, chitin 20-80, 0.05-0.2 percent triacontanol 400-1,000, hydrolyzed protein 2-5, potassium dihydrogen phosphate 300-400, thiourea 40-120, cobalt chloride 5-12, borax 50-100, sodium silicate 30-55, urea 300-500, ammonium molybdate 50-80, copper sulfate 30-65, zinc sulfate 120-200, magnesium sulfate 100-150, calcium chloride 50-120, manganese sulfate 40-80 and water in the amount of 3-8 times the total amount of all the raw materials or water in the amount capable of dissolving all the raw materials, wherein the water is de-ionized water or micro cluster water; and the preparation method is that all the materials are prepared into aqueous solution. The plant nutrient liquid fertilizer has the advantages of favorably solving the problems of over fertilization and shortage of nutrient elements in soil, obviously improving the plant adverse resistance, improving the photosynthesis and the accumulation efficiency of nutritive materials and improving the yield and the quality.

The invention provides a liquid biological organic fertilizer with high content of organic substances and a preparation method. The fertilizer is characterized by comprising the following components by weight: 60% of a humic acid release inhibitor, 0.4% of a composite biological agent, 10% of fulvic acid potassium, 12% of soluble nitrogen-phosphorus-potassium raw materials, 9% of a chelating medium trace element agent, 8.47% of a soil conditioner, 0.93% of compound sodium nitrophenolate and 0.1% of naphthalene acetic acid; and the fertilizer is prepared by mixing, heating, chelating, concentrating, cooling, microorganism adding, filtering and residue removing. The invention has the following beneficial effects: plant nutrition is directly supplemented; the contents of absorbable organic substances for a root system in soil are rapidly supplemented; growth of the root system is rapidly promoted; absorption area of the root system is enlarged; the chelating medium trace element agent can not be fixed by the soil and exists in a free state in the soil, so the chelating medium trace element agent is easy to be captured and absorbed by the root system in the soil; physiological diseases and pests are prevented from occurrence; quality of an agricultural product is improved; root-rot disease and stem-rot disease are prevented from occurrence; occurrences of fusarium and verticillium wilts and bacterial wilt are reduced; meanwhile, the fertilizer is capable of resisting salinity and alkalinity, resisting continuous cropping diseases, and killing soil-borne diseases.

The invention provides a panax ginseng adventitious root inducing culture medium and a method for inducing panax ginseng adventitious root. The panax ginseng adventitious root inducing culture mediumcomprises an MS basic culture medium, 0.2 mg / L-0.5 mg / L of IBA (indolebutyric acid), 2 mg / L-4 mg / L of NAA (naphthaleneacetic acid) and 20 g / L-30 g / L of sucrose. The panax ginseng adventitious root prepared by the method of the invention has a high content of ginsenoside, stable panax ginseng cell heredity and little influence by environmental factors, can be used as an ideal model for ginsenosidebiosynthesis molecule regulation research, and can be used for exploring an effective route for large-scale production of ginsenoside by engineered cells.

The invention discloses an apocarya hardwood cutting seedling raising method. The method comprises the steps of selecting a 12cm long middle section twig and a 12cm long lower section twig of an annual apocarya seedling, soaking the trimmed twigs in a solution of phytohormone IBA (Indolebutyric Acid) or NAA (Naphthaleneacetic Acid) for 4 hours, inserting the morphological lower ends of the twigs into a matrix, laying a heating wire at the bottom of the matrix, and controlling the temperature difference between the bottom of the matrix and the outside temperature at 15 DEG C approximately, and the water content at 30% approximately. The rooting rate is counted after 60 days, and reaches above 81%. The rooting rates of a single phytohormone treatment method or a single temperature difference method are both 0%. The method effectively realizes the apocarya cutting seedling raising; seedling raising approaches of apocarya are widened; and powerful technical support is provided for the rapid propagation and industrialization of the apocarya seedlings.

The invention relates to plant wound liquid containing chitosan oligosaccharide, which is characterized by comprising a plant growth regulator, wherein the regulator is one or more of naphthalene acetic acid and humic acid and accounts for 1 to 50 percent of the total weight of the liquid; and the chitosan oligosaccharide accounts for 0.0015 weight percent to 0.03 weight percent of the total weight of the liquid. The liquid can be prepared into any liquid preparation for spraying. One to five days before plants are trimmed, grafted or girdled, the liquid is used for spraying a wound. The liquid is used for apple trees, pear trees, peach trees, jujube trees, grape vines, mango trees, longan trees, lychee trees, mandarin trees, watermelons, gingko trees, lonicera japonica, jasmine, Chinese rose and the like.

The invention discloses a synergist of a functional fertilizer, comprising the following raw materials by weight: 0-150 portions of compound amino acid, 0-24 portions of plant growth regulator, 0-18 portions of synergistic sodium, 0-18 portions of a-nafusaku, 0-3 portions of antidote, 0-4 portions of pesticide, 0-600 portions of magnesium sulfate, 0-600 portions of nano calcium, 0-10 portions of zinc sulfate, 0-5 portions of ammonium molybdate, 0-15 portions of boric acid, 0-364 portions of adsorbent and 193-800 portions of bond. The preparation method comprises the following steps according to three proportions: adding water for mixing, rubbing, cutting, pelletizing, polishing, drying, cooling and screening, and then mixing the screened products with particle diameter less than 1 mm madeby three different proportions after equal part measuring by a computer, measuring, packaging and obtaining products. The invention can be used after mixing with a normal fertilizer easily and is suitable for various crops and soils, climates and different ways of fertilizing, thereby reducing the use level of the fertilizer and improving the utility ratio of the fertilizer. The invention has theadvantages of low use level, quick returns, stable fertilizer effect and long duration.

The invention discloses a modified medium for improving propagation of stems of Dendrobium officinale and a propagation method. The medium comprises MS (Murashige and Skoog) basal medium, cane sugar 20g / l, agar 6g / L, 6-BA (benayl aminopurine) 2mg / l, NAA (alpha-naphthaleneacetic acid) 0.2-0.5mg / l, banana mash 15-20g / l, potato mash 15-20g / l, and common tap water replacing distilled water. The modified MS medium is used to propagate the stems with axillary buds of Dendrobium officinale, alkaloid and polysaccharide content of Dendrobium officinale is evidently increased, and Dendrobium officinale grow normally. By the method, consumption of cane sugar is reduced, the tap water is used to replace the distilled water, and accordingly tissue culture technology for Dendrobium officinale is more convenient, more efficient and resource-saving.

The chemistry regulation and control cultivate means of the cowpea's high output and good quality is one of the cultivate means of the vegetable crop. Basing on the insufficiency of recent cowpea cultivate means, we dispose the cowpea at seedling stage and florescence by using plant growth regulator such as high yield element, drop-defending element and 1-naphthalene acetic acid, as well as cultivate synthetically by using cultivate measures such as scientific, rational manure and proper planting density, including nursery sock growing, planting, chemical controlled treatment, fertilizer water quality management, branching and wiping bud processes. It makes the output of the cowpea increase quickly, with the average increase production of 19percent to 31percent, the quality improves evidently, the content of ant scorbutic vitamin and reduction sugar increase, and the coarse fiber's content decreases. The invention is fit to different kinds of cowpea.

The invention discloses a cutting seedling method for malus toringoides. The cutting seedling method for the malus toringoides comprises the following steps of treating a cutting bed; laying cobblestones under the cutting bed; laying a window gauze on the cutting bed; laying mixing materials comprising nutrient soil and perlite on the window gauze; sterilizing soil; shearing semi-lignified branches, which grow normally, are not affected by plant diseases and insect pests and have plump buds, from a malus toringoides seed tree with the age about 10 years; timely bringing back the branches and manufacturing cutting slips; performing earlier-stage treatment on the cutting slips and performing cutting; and performing management after cutting. By using the cutting seedling method, the operation is simple, the yield is high, requirements on production of nursery-grown plants of the malus toringoides can be met, and the nutrient soil 3 and the perlite 1 serve as a cutting medium which is loose and breathable, has rich nutrition and can provide nutrition for the nursery-grown plants during earlier-stage growth. After the cutting slips are rapidly dipped in 1200mg / L of naphthaleneacetic acid liquor, cutting can be directly performed on the cutting slips, the cutting time is shortened, and the work efficiency is improved; the cutting yield is higher than 90%; the nursery-grown plants grow healthily and strongly; and the excellent performance of the maternal malus toringoides is kept. Compared with a seed raising method, the cutting seedling method has the advantages that cutting propagation is not limited by seasons, branches can be treated by cutting immediately after being shearing from the tree, and the operation is simple and feasible.

The invention discloses a saraca indica nutrient solution. The saraca indica nutrient solution in 1L of water comprises the following raw materials based on weight: 0.45-0.65mg of fulvic acid, 0.45-0.65mg of alginic acid, 0.55-0.75mg of alpha-naphthaleneacetic acid, 0.6-1.1g of nitrogen, phosphor and potassium compound fertilizer, 0.36-0.6g of microelements and 1.5-2mg of vitamin C. The saraca indica nutrient solution has the advantages of reasonable formula and stronger pertinence, and has the functions of quickly recovering saraca indica transplanted in a garden, shortening recovery time ofthe crown of the saraca indica and enabling the saraca indica planted in the garden to quickly grow up. The invention further discloses a preparation method of the saraca indica nutrient solution.

The invention discloses a culture medium for in-vitro induced regeneration plants of double-haploid stems of potatoes, which belongs to the field of agricultural biotechnologies. The culture medium comprises the following compositions: potassium nitrate, ammonium nitrate, monopotassium phosphate, magnesium sulfate, calcium chloride, potassium iodide, boric acid, manganese sulfate, zinc sulfate, sodium molybdate, copper sulfate, cobalt chloride, ferrous sulfate, disodium ethylenediamine tetraacetic acid, nicotinic acid, pyridoxine hydrochloride, glycine, glutamine, casein hydrolysate, yeast extracts, sucrose, 6-benzylaminopurine, naphthalene acetic acid, kinetin, zeatin and heteroauxin beilining. The culture medium is applied to the induction and culturing of double-haploid stem regenerated seedlings of potatoes, the test effect is good, the initiating rate is increased by 76%, and the seedling rate is as high as 80.5%. The regeneration rate of double-haploid stems of potatoes is significantly increased, thereby laying a foundation for the establishment of a genetic transformation system; and the culturing mode adopts a one-step seedling method, so that the pollution probability is reduced.

A lefa fertilizer dedicated for longan tree contains naphthaleneacetic acid, imidazolone, potassium dihydrogen phosphate, urea and trace elements (Zn, Cu, Mo, B, etc), and is prepared through proportionally mixing the pulverized naphthaleneacetic acid, imidazolone and potassium salt with water and filtering, proportionally mixing the pulverized molybdate, Cu salt, phosphate, urea, Zn salt, borax and complexing agent with water and filtering, proportionally mixing both filtrates, and adding water.

A plant growth regulator of cotton for promoting root growth and making plant strong is prepared from naphthaleneacetic acid, indolebutanoic acid, carbendazim, ammonium hydroxide (ammonia water), sodium hydroxide and water.

A nutrient solution for rejuvenating trees in gardens is a nutrient solution, containing macroelements, trace elements, organic substances, physiologically active substances and exogenous hormones required for the growth of plants, prepared from ammonium nitrate, potassium nitrate, urea, magnesium sulfate heptahydrate, potassium dihydrogen phosphate, calcium nitrate, calcium chloride dihydrate, sucrose, ferrous sulfate heptahydrate, disodium ethylenediaminetetraacetate, zinc sulfate, copper sulfate, manganese sulfate tetrahydrate, sodium chloride, boric acid, vitamin A, vitamin C, vitamin B1,vitamin B6, humic acid, naphthaleneacetic acid, indolebutyric acid, 6-benzyladenine and gibberellin. The nutrient solution for rejuvenating trees in gardens can improve the survival rate of transplanted trees and promote and accelerate the self-recovering ability of nursery plants in order to make the nursery plants reach an expected landscape effect. The nutrient solution has a good rejuvenatingeffect on famous and old trees planted for many years and having poor growth vigor, and can enhance the resistance, break the dormancy, promote the germination of new shoots and new sprouts and delayaging.

The invention discloses organic-inorganic compound fertilizer capable of improving a soil environment. The fertilizer is prepared from raw materials in parts by weight as follows: 20-40 parts of urea, 5-15 parts of calcium ammonium nitrate, 10-20 parts of precipitated phosphate fertilizer, 6-16 parts of monopotassium phosphate, 10-20 parts of potassium sulfate, 40-60 parts of fermented chicken manure, 5-15 parts of fermented pig manure, 2-6 parts of bamboo powder, 2-8 parts of shell powder, 10-20 parts of tapioca flour, 5-15 parts of corncobs, 4-8 parts of modified fly ash, 5-12 parts of oil shale powder, 2-4 parts of montmorillonite powder, 2-6 parts of borax, 1-2 parts of sodium silicate, 0.5-1.5 parts of ammonium molybdate, 0.4-0.9 parts of zinc carbonate, 0.1-0.8 parts of magnesium sulfate and 0.5-2 parts of sodium naphthalene acetate. The fertilizer contains very rich nutrients and can decrease the release speed of organic nutrients, effectively enhance soil fertility, effectively improve the soil structure and make hardened soil loose.

The invention relates to a cultivating method of rose nutrient solution. The cultivating method includes A, planting, selecting plants, cutting all the fiber, reserving 3-5mm main roots, using carbendazim to immerse and disinfect, and immersing in naphtylacetic acid aqueous solution; B, planting in field, planting the plants with roots processed into a planting basket with 5-10mm depth and through holes; C, catalyzing roots, transferring the plants with the planting basket into perlite matrix, and controlling environment conditions via an automatic controlling system to catalyze new roots of the plants;D, accelerating roots, immersing into a nutrient solution water groove, accelerating the top of the roots of the plants generating new aquatic roots capable of suiting water via the automatic controlling system to control environment conditions so as to become commercial plants. The cultivating method has the advantages that 1, cultivating time is greatly reduced, 2, the plants are easy to be controlled by intelligent systems, so that factor-scale production can be realized and 3, plant diseases and insect pests are few and survival rate is greatly increased.

The invention discloses a method for using biogas slurry to prepare a liquid manure containing humic acid. The preparing method of the liquid manure containing the humic acid comprises the steps of uniformly mixing potassium nitrate, potassium fulvic acid, ferrous sulfate, borax, diethyl aminoethyl hexanoate and naphthaleneacetic acid sodium salt in proportion to obtain a base fertilizer containing the humic acid and major elements, uniformly mixing the base fertilizer, the biogas slurry, a chelating agent and a stabilizer in proportion, continuously heating and stirring the mixture to obtain a black viscous liquid manure, cooling the liquid manure and uniformly mixing the liquid manure with diammonium hydrogen phosphate, ammonium nitrate phosphate, and amino acid to obtain the liquid manure containing the humic acid. The liquid manure containing humic acid developed from the method can not only achieve full amount of use of the biogas slurry, and can also promote an additional value of the biogas slurry; meanwhile, the prepared liquid manure product is good in quality, and is high in content of the humic acid, content of nitrogen phosphorous and content of potassium; in addition, the liquid manure containing humic acid developed from the method has the advantages that the processing technology is simple in procedures and efficient.

The invention belongs to the technical field of plant biology, and particularly discloses a regeneration culture medium and a culture method for improving regenerated adventitious buds of echinacea explants. According to the regeneration culture medium, corresponding concentration of DA-6 (diethyl aminoethyl hexanoate) is also especially added besides that 15-60g / L of cane sugar, 3-9g / L of agar, 0.1-1.5mg / L of BA (benzylaminopurine) and 0.01-0.2mg / L of NAA (naphthaleneacetic acid) are added to an MS (Murashige and Skoog) formula aiming at difference of different ploidies and different sources of echinacea explants on the sensitivity to diethyl aminoethyl hexanoate (DA-6). By adopting the regeneration culture medium, the effect of facilitating regeneration of BA-induced adventitious buds is significantly enhanced by adding DA-6. By adopting the regeneration culture medium and the culture method disclosed by the invention, the problem of different genotype limits in regeneration cultivation of echinacea can be effectively overcome; the regeneration efficiency of the adventitious buds is greatly improved; development of relevant research work of biotechnology breeding of the echinacea is facilitated.

The invention discloses a winter wheat growth regulator. The winter wheat growth regulator is divided into a first regulator used before seeding or in the later-seedling stage, a second regulator used from the tillering stage to the elongation stage, and a third regulator used after elongation, and the three regulators are sequentially used according to the growth stage of wheat. The second regulator is mainly composed of, by weight, 2.0-2.2% of naphthalene acetic acid, 0.58-0.78% of compound sodium nitrophenolate, 0.01-0.15% of natural brassinolide, 0.03-0.1% of uniconazole, 0.035-0.06% of choline chloride and the balance of water. The growth regulator is efficient, wide in spectrum, free of public nuisance and environmentally friendly, the naphthalene acetic acid and the compound sodium nitrophenolate with different concentrations are combined and used in the whole growth stage of the wheat, and then, a proper amount of natural brassinolide and uniconazole are added. The winter wheat growth regulator is used in the key growth stage of the wheat, the number of secondary roots can be increased, the activity of the roots can be enhanced, dipping penetration and growth of the roots are promoted, the capacity of absorbing various nutrients and water of the roots is enhanced, and therefore the drought resistance of the wheat is improved, and the winter wheat growth regulator is safe and efficient.

The invention relates to a Hongsenhuai sophora softwood cutting propagation technology comprising the following steps: (1) a seedling bed is constructed, wherein a bed is arranged in a nursery shed, and a cutting medium is arranged on the seedling bed; (2) a cutting period is selected, wherein cutting is carried out around May to August; (3) cutting clipping and treatment are carried out, wherein pruning shears used for cutting softwoods should be sterilized and disinfected by using 95% ethanol; cutting is carried out after the pruning shears are air-dried; 1 / 3000 carbendazim is sprinkled 2h before cutting insertion until the medium is thoroughly wetted; and the softwoods are soaked for 10min by using 1 / 3000 carbendazim and 3000ppm naphthaleneacetic acid; and (4) the softwoods are inserted into the seedling bed medium with spacings of 8-10cm, wherein the insertion depth is 2 / 3-3 / 4 of the lengths of the cuttings, and that one bud of each cutting is maintained above ground. With the Hongsenhuai sophora softwood propagation technology, a technical problem of Hongsenhuai sophora summer propagation is solved, such that Hongsenhuai sophora nursery can be carried out all year. With the method, Hongsenhuai sophora fast propagation is facilitated, and female parent excellent features are maintained.

The invention relates to a method for rapid release of Callicarpa nudiflora seed dormancy. The method comprises the following steps: 1) impregnating Callicarpa nudiflora seeds for 12-24h with one or more selected from 5-20 wt% of a plant ash water solution, 100-300mg / L agibberellin (GA3) aqueous solution and 100-300mg / L alpha-naphthaleneacetic acid (NAA), and then washing the seedswith water; and step 2) germinating the impregnated seeds in the step 1). The method for rapid release of Callicarpa nudiflora seed dormancy provided by the invention can effectively relieve various factors hindering germination of the Callicarpa nudiflora seeds, including mechanical barrier formed by the seed coat, break Callicarpa nudiflora seed dormancy, and enhance seed germination rate to 95%. Compared with a traditional seedling raising way without treatment, the method has advantages of shortened breeding cycle, uniform seedlings, and simple operation and low cost, and can be widely used for breeding and cultivation of a Callicarpa nudiflora medicinal material.

The invention discloses moisture-holding nutritive soil and a preparation method thereof. The method comprises the following steps: weighing citric acid, alpha-naphthaleneacetic acid, corn starch, copper sulfate powder, zinc sulfate powder, borax powder, ferrous sulphate powder and water according to a mass ratio respectively; mixing the raw materials; stirring the mixed raw materials for 30 to 40 minutes at a rotation speed of 100r / min and at a reaction temperature of 80 to 90 DEG C and obtaining micro element chelating liquid for later use; and weighing oil shale dust, furfural slag, calcium carbonate, magnesium sulfate, urea, diammonium hydrogen phosphate, potassium sulphate and acrylic resin water absorbent respectively according to a mass ratio, drying and crushing the raw materials, passing the crushed materials through 100 to 120-mesh sieve, feeding the obtained materials in a stirrer, spraying the micro element chelating liquid in an amount which is 10 to 15 percent based on the mass of the raw materials into the raw materials with stirring, uniformly stirring and obtaining the moisture-holding nutritive soil. The moisture-holding nutritive soil has the advantages that: the nutrients are sufficient; the process is simple; the raw materials are readily available; the micro elements can be absorbed and used by the plants conveniently; water supply capacity and time are improved and prolonged; and the like.

The invention provides a pervaporation hybridigation composite film, which consists of a separation layer and a support layer, wherein the separation layer is a mixture of hydrophilic NaA (naphthaleneacetic acid) and PVA (polyvinyl acetate). The pervaporation hybridigation composite film has the advantages that the separation performance is good, in addition, the mechanical intensity is high, the materialization stability is good, the preparation process is simple, the large-area preparation is easy, the further amplification is favorably realized, and the industrial production is finally realized. The invention also provides a preparation method of the composite film and application to the separation of mixtures of organic matter and water.

The multicomponent composite liquid fertilizer is made up by using titanium tetrachloride, vitamin C, ammonia water, potassium hydroxide, naphthaleneacetic acid and water as main raw material according to a certain proportion, and adding the auxiliary materials of sodium benzoate, sodium dodecyl benzene sulfonate, trace element fertilizer and urea. It possesses the advantages of complete nutrients, reasonable mixing ratio, stable product, obvious yield-increasing effect and convenient application. The application of said liquid fertilizer can activate plant cell growth, promote metabolism, supplement plants with nutrients, increase enzyme content of plant and raise photosynthesis of plant. Said liquid fertilizer is applicable to various crops.

The invention provides an embryo culture medium for Lei bamboo (Phyllostachys praecox), which comprises the following components by weight part: MS salts, MT vitamins, 0.3 mg / L or 1 mg / L or 3 mg / L of BA (i.e. benzylamino purine), 0.1 mg / L of NAA (i.e. alpha-naphthaleneacetic acid), 0.1 mg / L of 3,5,6-trichloro-4-aminopicolinic acid, 0.001 mg / L of TDZ (i.e. thidiazuron), 20 g / L of glucose, 100 mg / L of inositol, 10 mg / L of adenine sulfate, and 0.5 g / L of malt extract. The pH value of the culture medium ranges from 5.0 to 6.0. The method of tissue culture and seedling cultivation using the culture medium comprises the following steps: collection and selection of explants, cleaning and sterilization of seeds, and extraction, inoculation, culture, sub-culture or transplanting of the embryos. The culture medium and the seedling tissue culture method are excellent in the cultivation of the Lei bamboo as the medium-diameter running bamboo with high economic value, lay the foundation for researching and bioengineering of Lei bamboo, and realize the rapid and sufficient supply of Lei bamboo seedlings.

Disclosed is a continuous-cropping bacteria resistant agent. The continuous-cropping bacteria resistant agent is composed of bacillus licheniformis bacteria powder, streptomyces microflavus powder, pythium oligandrum yeasts, green wooden mold powder, edible fungi residues, compound sodium nitrophenolate and alpha-naphthalene acetic acid. By the arrangement, continuous-cropping diseases of crops can be alleviated, diseases of bacterial wilt, tobacco mosaic and the like caused by continuous cropping are highly prevented, various diseases of thielariopsis basicola, blight, root-knot nematode, damping off and the like can be prevented, and incidence rate and disease index of flue-cured tobacco black shank disease, the thielariopsis basicola and common mosaic are effectively reduced; by the aid of the continuous-cropping bacteria resistant agent, flue-cured tobacco can be promoted in early growth and quick tiller, growth period is brought forward, plant height and stem thickness of the flue-cured tobacco are increased, yield of flue-cured tobacco leaves is increased, and appearance quality of the tobacco leaves is improved.

The invention discloses a method for using Lycoris radiata to induce cluster buds, which includes: subjecting a bulb of Lycoris radiate to cuttage into a substrate, and culturing for 60-90 days to obtain a tender small bulb; and inoculating the small bulb sterilized to a liquid medium for induced culture; after buds appear in induction, transferring the buds to a solid medium for enrichment culture, and obtaining cluster buds; wherein the liquid medium contains 0.5-1.0mg / L alpha-naphthaleneacetic acid and 3.0-5.0mg / L 6-benzylaminopurine. The bulb of Lycoris radiate is subjected to cuttage propagation to obtain the mall bulb used as explant material, and the explant is tender, convenient in massive obtaining and low in pollution rate. In addition, by induced culture, liquid suspension culture, amount of emergent buds is large, emergency is quick, and a great amount of quality cluster buds can be obtained after one month. Application of the method attains the advantages that budding time is short, budding rate and proliferation rate are high, the small bulb is fast in growth and development, and the like.

The invention provides tissue culture and rapid propagation technology for wine bamboo. The technology comprises the steps of choosing and processing of an explant, induction culture, enrichment culture, rooting culture, transplantation and the like. In the technology, auxiliaries including active carbon, 6-benzyladenine, 6-furfurylaminopurine and naphthylacetic acid are added into an MS culture medium serving as a basis for the tissue culture in two key stages of the induction of the growth of cluster buds and the rooting of strong seedlings of the wine bamboo. The technology solves problems at the important technical links of the rapid propagation and the stable rooting of the wine bamboo and fulfills the aim of high propagation coefficient, stable technology and industrial production.