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Modified medium for improving propagation of stems of Dendrobium officinale and propagation method

A technology of Dendrobium officinale and culture medium, which is applied in the agricultural field, can solve the problems of large power consumption, waste, increased cost burden, etc., and achieves the effects of saving production time, reducing production costs and facilitating production needs.

Inactive Publication Date: 2012-08-01
NANJING AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Since the 1970s, relevant institutions at home and abroad have started research and development of Dendrobium candidum, especially in the field of tissue culture technology of Dendrobium candidum. The preparation of all requires special instruments and consumes a lot of power, which increases the cost burden and causes a lot of waste

Method used

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  • Modified medium for improving propagation of stems of Dendrobium officinale and propagation method
  • Modified medium for improving propagation of stems of Dendrobium officinale and propagation method
  • Modified medium for improving propagation of stems of Dendrobium officinale and propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1 Under aseptic conditions, take the stem section of Dendrobium officinale with axillary buds of 1.5 to 2 cm in length with 3 to 4 stem sections as an explant (derived from the aseptic seedling of Dendrobium officinale), and prepare the After the improved medium (pH 5.8) was subjected to high-temperature sterilization, the stem section of Dendrobium candidum was placed in the improved medium prepared by tap water at a temperature of (25±2)°C, an illumination of 1500-2000lx, and a light time of 12h / d. Cultivate under conditions for 30 days, measure and record the polysaccharide content of Dendrobium officinale in the improved medium prepared by ordinary tap water, and carry out the next subculture after 30 days of cultivation, and then transplant the hardened seedlings.

[0016] Components of the modified medium prepared from tap water (tap water to 1 L):

[0017] Composition

Embodiment 2

[0018] Example 2 Under aseptic conditions, take the stem section of Dendrobium officinale with axillary buds of 1.5 to 2 cm in length with 3 to 4 stem sections as an explant (aseptic seedling derived from Dendrobium officinale), and prepare the After the improved medium (pH5.6) was sterilized at high temperature, the stem section of Dendrobium candidum was placed in the improved medium prepared by tap water at a temperature of (25±2)°C, an illumination of 1500-2000lx, and an illumination time of 12h / d. Cultivate under conditions for 35 days, measure and record the polysaccharide content of Dendrobium officinale in the improved medium prepared by ordinary tap water, and carry out the next subculture after 30 days of cultivation, and then transplant the hardened seedlings.

[0019] Components of the modified medium prepared from tap water (tap water to 1 L):

[0020]

Embodiment 3

[0021] Example 3 The stem section of Dendrobium officinale with axillary buds was prepared with distilled water for tissue culture according to the method of Example 1, and the culture time was 40 to 50 days.

[0022] Components of MS medium prepared with distilled water (distilled water to 1L):

[0023]

[0024]

[0025] Extraction method of Dendrobium officinale polysaccharides: Take dry samples of stems of Dendrobium officinale after tissue culture, accurately weigh an appropriate amount, reflux and degrease through petroleum ether (60-90°C) for 1h, filter, evaporate the solvent, and extract with 80% ethanol for 1h under reflux , filter by heat, add water to the filter residue and reflux to extract for 1 hour, filter by heat, concentrate the filtrate, add 0.1% activated carbon for decolorization, filter, add 95% ethanol to make the solution contain 80% alcohol, let it stand overnight, filter, use absolute ethanol, acetone After washing 8 times in sequence, the obtaine...

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Abstract

The invention discloses a modified medium for improving propagation of stems of Dendrobium officinale and a propagation method. The medium comprises MS (Murashige and Skoog) basal medium, cane sugar 20g / l, agar 6g / L, 6-BA (benayl aminopurine) 2mg / l, NAA (alpha-naphthaleneacetic acid) 0.2-0.5mg / l, banana mash 15-20g / l, potato mash 15-20g / l, and common tap water replacing distilled water. The modified MS medium is used to propagate the stems with axillary buds of Dendrobium officinale, alkaloid and polysaccharide content of Dendrobium officinale is evidently increased, and Dendrobium officinale grow normally. By the method, consumption of cane sugar is reduced, the tap water is used to replace the distilled water, and accordingly tissue culture technology for Dendrobium officinale is more convenient, more efficient and resource-saving.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to an improved culture medium for promoting the proliferation and multiplication of dendrobium candidum stems and a method for using the culture medium to carry out multiplication and multiplication. Background technique [0002] Dendrobium officinale (Dendrobium officinale) is a perennial herb belonging to epiphytic orchids. It is listed as the top grade in Shennong’s Herbal Classic, and it has been confirmed that it has the functions of nourishing yin and clearing heat, promoting body fluid and benefiting stomach, relieving cough and moistening lung, moistening throat and improving eyesight, improving human immune function, preventing tumor and cardiovascular disease, improving sleep, preventing cataract, Prolong life and other effects. However, due to the special growth environment, limited distribution, and long-term mining, natural resources are becoming increa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 杨岚向增旭
Owner NANJING AGRICULTURAL UNIVERSITY
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