85 results about "Exogenous hormones" patented technology

The invention provides an efficient subculture and rapid propagation method for ceratostigma willmottianum. The efficient subculture and rapid propagation method includes steps of shearing aseptic seedlings of ceratostigma willmottianum offspring to obtain explants, placing the explants in culture media without exogenous hormones and carrying out expanding propagation and proliferation culture onthe explants. The aseptic seedlings of the ceratostigma willmottianum offspring are obtained by means of preliminary tissue culture and breeding; each stem of each explant is provided with a leaf. Theculture media without the exogenous hormones are MS culture media with added sucrose and added agar. A preliminary tissue culture and breeding process includes acquiring tender stems of the upper half portions of ceratostigma willmottianum branches and utilizing the tender stems with buds as explants; sequentially carrying out auxiliary bud induction culture, auxiliary bud proliferation culture and rooting culture. The efficient subculture and rapid propagation method has the advantages that the breeding production cycle of the ceratostigma willmottianum can be shortened, and the production cost can be reduced; the efficient subculture and rapid propagation method has a large propagation coefficient and can be easily and feasibly implemented, the ceratostigma willmottianum can quickly grow, and materials can be acquired at any time, so that aseptic rapid propagation can be carried out.

The invention relates to a method for producing alkannin by utilizing Arnebia euchroma(Royle)Johnst hairy root. The method includes selection and processing of Arnebia euchroma(Royle)Johnst explant, activation culture of agrobacterium rhizogene, infection of cotyledon explant and induction of hairy root, obtaining of sterile Arnebia euchroma(Royle)Johnst hairy root system and liquid culture of Arnebia euchroma(Royle)Johnst hairy root as well as alkannin production. The used hairy root has the characteristic of rapid and autonomous growth on culture medium without exogenous hormones, thus the method not only can overcome the defects that cell culture grows slowly and hormone is required to be additionally added to maintain the growth thereof when utilizing cell culture to produce alkannin,and the method has the advantages of stable alkannin throughput, simple operation in cultivation, lower cost and no restriction of climate and land natural conditions. Especially the invention provides a stable and continuable novel medicine source for producing alkannin by utilizing hairy root culture method and provides reliable source and material basis for alkannin mass production by applyingbioreactor in the future.

The invention discloses a cutting propagation method of cryptomeria fortunei. The method includes the following steps that 1, cutting pretreatment is conducted; 2, exogenous hormones NAA50-150 ppm + IBA100-300 ppm are used, and cuttings are soaked for 0.5-1.5 h; 3, cutting is conducted; 4, after-management of cutting is performed. According to the cutting propagation method of cryptomeria fortunei, it is proved by experiments that under the conditions that exogenous hormones NAA150ppm + IBA100ppm are adopted and the cuttings are soaked for 1.5h, the rooting rate of the cryptomeria fortunei cuttings can reach 98.33%, the survival rate of the cuttings is increased, and the root growth can be promoted. The method is simple and feasible, and reliable seedling sources are provided for fast-growing and high-yield cryptomeria fortunei forest planting.

The invention relates to an artificial propagation method of yellow catfish fry, which is characterized in that: sexually mature yellow catfish over three years are chosen as the parent fishes; the female and male ratio is 3:1; a plurality of barrels are arranged in a spawning pond as fish nests; parent fish density in the spawning pond is 1 to 2 groups / m2; each group of parent fishes uses a barrel; the sand with a thickness ranging form 1cm to 5cm is arranged at the bottom of the barrel for the debonding of fish eggs; in spawning season, exogenous hormone is injected to the basal behind the dorsal fins of the parent fishes to induce ovulation and spermiation; an inversed conical container with smooth inner wall is used as the hatching barrel; fertilized eggs are arranged in the hatching barrel for hatching in flowing water; the dissolved oxygen in the water of the hatching barrel is larger than 5.5 mg / L; when hatching is over, fry is arranged in a cement pool for temporary rearing for 40 to 70 hours, then the fry is packaged in plastic bags with water and oxygen. The artificial propagation method of yellow catfish fry has the advantages that, well developed yellow catfish fry canbe acquired; the operation is simple; the utilization rate of parent fishes is high; scale production of yellow catfish fry can be realized, and the invention has significant meaning for the intensive culture of yellow catfish.