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In-vitro grafting method

A technology of in vitro grafting and grafting seedlings, applied in the agricultural field, can solve problems such as affecting the speed and efficiency of seedlings, long seedlings cycle, and cultivate a large number of seedlings, and achieve the effect of improving the healing survival rate and high survival rate.

Inactive Publication Date: 2015-03-25
四川中天地木业有限公司
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  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cultivation time of the rootstock plus the cultivation time of the grafted seedlings makes the entire seedling cultivation cycle too long. Some varieties need 2-3 years, and the short cycle takes 1 year, which greatly affects the speed and efficiency of seedling cultivation.
Moreover, a rootstock tree can only be grafted once, and a large number of rootstock trees need to be cultivated for grafting.
In addition, the survival rate of traditional grafting is greatly affected by the external environment, such as changes in climate factors such as unstable humidity, temperature, and light, which will affect the survival rate, especially in rainy weather or high temperature and drought. greatly reduced

Method used

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  • In-vitro grafting method

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Embodiment 1

[0018] A kind of in vitro grafting method, comprises the following steps:

[0019] 1) Select rootstock varieties and scion varieties adapted thereto: pumpkin seedlings can be selected as rootstocks, melon seedlings as scions, and the plant height of pumpkin seedlings is similar to that of melon seedlings.

[0020] 2) 3-5 scions are grafted onto a rootstock by grafting method, and then the rootstock on which the scions are grafted is cut off to carry out disinfection and root soaking;

[0021] Remove the true leaves of the pumpkin seedlings, put the pumpkin seedlings and melon seedlings close together, and then select two branches with similar thickness to separate the pumpkin seedlings and melon seedlings. Use a blade along the parallel direction of the pumpkin branches and leaves, at an angle of 20-30 degrees Cut a plane 3 to 5 cm long to expose the cambium and xylem. Similarly, the cut surface of the melon seedlings can be connected with the cut surface of the pumpkin seedli...

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Abstract

The invention discloses an in-vitro grafting method. According to the in-vitro grafting method, a scion and a stock are both in-vitro materials. After grafting, the stock which the scion is grafted onto is rapidly transplanted to a seedling bed, and the purpose that rooting of the stock and union of a connector are synchronous is achieved. By the adoption of the in-vitro grafting technology, grafting is easy to operate, centralization and industrialization of grafting are achieved, and multiple varieties with a low survival rate during traditional grafting or distant varieties can have a high survival rate after the environment optimization technology is adopted; especially, a good promoting function is achieved on inducing of callus tissue of a cut and formation of a fusion vascular bundle between heterogeneous cells, supplementation of a nutrient solution and exogenous hormones can be achieved in the integrally optimized seedling bed, and the union survival rate is greatly increased; the in-vitro grafting method has also the positive function on rapid cultivation of various induced seedlings during breeding.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to an in vitro grafting method. Background technique [0002] Traditional grafting is carried out on the rootstock of planting in the ground mostly, and after grafting must wait for the grafted branches to survive before transplanting. The cultivation time of rootstock plus the cultivation time of grafted seedlings makes the entire seedling cultivation cycle too long, some varieties need 2-3 years, and the short cycle also needs 1 year, which greatly affects the speed and efficiency of seedling cultivation. And a rootstock tree can only be grafted once, and a large number of rootstock trees need to be cultivated for grafting. In addition, the survival rate of traditional grafting is greatly affected by the external environment, such as changes in climate factors such as unstable humidity, temperature, and light, which will affect the survival rate, especially in rai...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/06A01G7/06
CPCA01G2/30
Inventor 高仕鹏王涛杨慧茹
Owner 四川中天地木业有限公司
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