The invention discloses a mixed culture base material for pleurotus eryngii culture. The base material comprises the following components in parts by weight: 3 to 5 parts of nutritional inorganic fertilizer, 60 to 70 parts of weed tree sawdust, 20 to 90 parts of oat husk, 10 to 40 parts of wood bran, 10 to 40 parts of peanut shell, 5 to 15 parts of cotton seed husk, 26 to 30 parts of calcium carbonate, 35 to 40 parts of corn core, 10 to 20 parts of sugarcane dreg, 15 to 25 parts of wheat bran, 3 to 8 parts of corn flour, 20 to 30 parts of calcium sulfate, 5 to 10 parts of magnesium sulfate, 5 to 10 pats of lime, 0.25 to 4 parts of gypsum, 5 to 10 parts of urea with a nitrogen content of 46%, 5 to 10 parts of triacontanol, and 2 to 7 parts of soybean meal. The water content of the mixed culture material after pre-wetting is 65% + / - 1%, and the pH value is 7.5 to 9. The nutritional inorganic fertilizer is a boron-potassium mixed fertilizer, and the mass ratio of boron fertilizer to potassium fertilizer is 1:1.5. The provided mixed culture base material is rich in nutrients such as vitamin B1, vitamin B, crude fiber, proteins, mineral substances, trace elements, and the like, and is beneficial for the growth of edible fungi. The edible fungi cultured by the provided mixed culture base material have a fresh and sweet flavor and a crispy and smooth taste.