178 results about "6-benzyladenine" patented technology

The invention discloses a method for optimizing a potato isolated culture one-step seedling culture medium. The culture medium makes leaves of a potato test tube plantlet undergo isolated culture to form a seedling in one step. The one-step seedling culture medium takes an MS culture medium as a basal medium, and 6-benzyladenine, naphthyl acetic acid and 2,4-dichlorphenoxyacetic acid with different concentrations and combinations are supplied in 1L of the MS culture medium; adventitious buds and adventitious roots are directly differentiated through callus induction; and the potato regenerated seedling is obtained in one step. The callus obtained by induction in a primary culture medium is unnecessarily inoculated to a differential medium for regenerating the seedling. Compared with an isolated culture multi-step regenerated seedling method, the method of the invention has the advantages of simplified steps, short culture period, high repeatability, and high seedling survival rate.

An in-vitro culture method for catalpa bungei comprises 1) inducing callus, concretely, putting catalpa bungei on a callus induction medium for inducing callus, wherein the callus induction medium is obtained by adding 6-benzyladenine (6-BA) and 2,4-dichlorophenoxyacetic acid (2,4-D) on the basis of 1 / 2 MS basal medium; 2) propagating callus, concretely putting callus on a propagation medium for propagation, wherein the propagation medium is obtained by adding 6-benzyladenine (6-BA) and alpha-naphthalene acetic acid (NAA) on the basis of 1 / 2 MS basal medium; 3) inducing embryonic callus and differentiating somatic embryo, concretely putting propagated callus on an induction differentiation medium, wherein the induction differentiation medium is obtained by adding 6-benzyladenine (6-BA) and alpha-naphthalene acetic acid (NAA) on the basis of 1 / 2 MS basal medium; and 4) regenerating a plant, concretely putting somatic embryo obtained through differentiation on a regeneration medium for inducing somatic embryo to germinate, so as to obtain a catalpa bungei regeneration plant. The method aims at expanding catalpa bungei propagation coefficient and providing conditions for germplasm preservation and genetic transformation.

Belonging to the field of plant propagation, the invention discloses a paper mulberry seedling tissue culture method suitable for extensive plantation. After top plumules are grown into buds on germination medium, the buds are inoculated onto bud induction medium for propagation, axillary buds and adventitious buds are grown out, so that rootless seedlings are obtained, and the healthy rootless seedlings are then transferred onto rooting medium for adventitious root induction. The components of the mediums are as follows: germination medium: DCR; bud induction medium: 6-BA and NAA are added to the germination medium; and root induction medium: 6-benzyladenine (6-BA), Alpha-naphthylacetic acid (NAA) and indolebutyric acid (IBA) are added to the germination medium. After induced propagation, the majority of adventitious bud leaves are thick, stems are bulky, and after multiple times of subculture, the buds grow normally. The paper mulberry seedling tissue culture method has the advantages of low mutation rate and high propagation coefficient, the survival rate of acclimatized seedlings reaches more than 90 percent, and the paper mulberry seedling tissue culture method is suitable for providing seedlings for the extensive plantation of paper mulberries, and can bring good economic and social benefits.

The invention discloses a tissue culture and rapid propagation technology for Clematis 'Arabella', which belongs to the technical field of tissue culture and rapid propagation of the Clematis 'Arabella'. In the technology, a regeneration system of the Clematis 'Arabella' is established by taking a stem segment, which is provided with buds, of a new branch in the current year as an explant. The research discovers that: the optimal culture medium for axillary bud induction comprises a murashige and skoog (MS) culture medium, 2,4-dichlorophenoxyacetic acid at the concentration of 1.0mg / L, indolebutyric acid at the concentration of 1.0mg / L and sucrose at the concentration of 30g / L, and the differentiation rate reaches 100 percent; the optimal culture medium for adventitious bud proliferation comprises an MS culture medium, 6-benzyladenine at the concentration of 0.5mg / L, indolebutyric acid at the concentration of 0.1mg / L and sucrose at the concentration of 30g / L, and the proliferation coefficient is 3.98; and an appropriate rooting culture medium comprises a 1 / 2MS culture medium and naphthylacetic acid at the concentration of 0.04mg / L, and the rooting rate reaches 89.3 percent. Through 5 to 7 days of bottle-opening seedling hardening, pearlite and peat in a ratio of 1:3 are taken as matrices, and after 20 days, the investigation discovers that the transplant survival rate of seedlings reaches 90 percent. The technology can be applied to the efficient and rapid propagation of the Clematis 'Arabella', and can realize the industrial breeding of the Clematis 'Arabella'.

The invention discloses a celtis sinensis scion rooting liquid and a celtis sinensis grafting and seedling raising method and solves the problem that the rooting liquid is liquid and extremely easy tolose in a use process to affect the survival rate of the raised celtis sinensis. The technical scheme is characterized in that the rooting liquid comprises the following components in parts by mass:10-20 parts of vegetable gum, 15-20 parts of indoleacetic acid, 15-20 parts of naphthylacetic acid, 2-4 parts of 6-benzyladenine, 1-5 parts of nutrimental agent, 1-2 parts of bacteriostat, 0.1-0.5 part of acetylsalicylic acid, 0.1-0.5 part of vitamin B12, 0.1-0.5 part of vitamin D and 0.1-0.5 part of honey. The loss rate of the rooting liquid in a use process is reduced.

The invention provides a Chuanminshen violaceum tissue culture method. According to the method, the stem tip, tender leaves and fresh tender leaf stalks of the Chuanminshen violaceum plant with good characters in the vigorous growth period are utilized as explants, and the method comprises the following steps of: performing disinfection treatment and inoculation induction to form callus; performing callus multiplication, bud induction culture and rooting culture to obtain complete Chuanminshen violaceum plant seedling; and transplanting the complete seedling into a seedling culture medium, and culturing to obtain a Chuanminshen violaceum annual seedling. All the culture media are based on an MS culture medium in combination with components such as 6-benzyladenine, naphthylacetic acid, 2,4-dichlorphenoxyacetic acid, indolebutyric acid, sucrose an agar. The method provided by the invention can be used for realizing the tissue rapid propagation of Chuanminshen violaceum by use of a plant tissue culture technology, and has important application values in terms of annual seedling supply and improved-variety rapid expanding propagation of the industrialized cultivation of Chuanminshen violaceum.

Soluble and stable liquid compositions containing a plant growth regulator selected from the group consisting of cytokinin and a gibberellin, an acid solubilizer such as citric acid, tartaric acid or glycolic acid and a solvent; as well as methods for making and using the composition are disclosed. The compositions improve solubility, handling, stability, safety, as well as activity improvements such as improved plant growth, yield, fruit thinning or sizing and quality. The compositions are soluble and stable by adding an ethoxylated alkyl alcohol wherein the growth regulator is 6-benzyladenine (6-BA) or forchlorfenuron (CPPU) and the ethoxylated alcohol surfactant is C12-15 alkyl alcohol in propylene glycol. The compositions may also contain a cytokinin such as 6-benzyladenine (6-BA) or forchlorfenuron (CPPU) that is increased in solubility and activity and by synergistically combined with GA3 or GA4A7 as well as in storage stability by adding an antioxidant. The compositions are formulated in a ready-to-mix formulation.