72 results about "Syringin" patented technology

The invention relates to an application of Saussurea involucrata culture as a new raw material in daily chemicals, belonging to an application of pure vegetal raw material in the field of daily chemicals. The Saussurea involucrata culture is characterized by comprising the following active ingredients: flavone, polyose, chlorogenic acid, Syringin, trace elements, terpene and the like. The invention does not have damage to the hair and skin of human bodies, has strong practical applicability, wide application range and no pollution, and can not damage the natural resources of the Saussurea involucrata, can be used as a vegetal active matter to be added into the daily chemicals, and has remarkable effect in the aspects of eliminating the inflammation, preventing oxidation, delaying senility and blocking ultraviolet rays. The advised additive amount of the Saussurea involucrata culture is 0.01-10%.

The invention relates to a medicament detection method, in particular to a panax root extract detection method. The detection method comprises: measuring the content of a syringin component; and comparing the intermediate infrared one-dimensional spectrum of the panax root extract and the intermediate infrared one-dimensional spectrum of a reference product.

The invention relates to a method for extracting and separating ligustrin, which is to extract the ligustrin by the decoction method through crushing of excrementum passeris (Syringa oblata Lindl.var. alba Hort.ex Rehd.) branches. A ligustrin extract is eluted by 20 to 60 percent alcohol solution through an XDA-1 or AB-8 macroporous absorption resin column, and undergoes chromatographic separation through a silica gel column, and then ligustrin crystals with the purity over 97 percent can be obtained. The method comprises the following techniques: extraction of the ligustrin from the excrementum passeris, separation of macroporous resins and the silica gel column, crystallization and so on. The method has the advantages of low cost, simple and convenient operation, convenient industrialized production and so on. Both the stability and the reproducibility of the techniques are good; the purity of products obtained is more than 97 percent; and the method is suitable for extraction in mass production.

The invention relates to a low-toxicity acanthopanax injection and a preparation method thereof. The acanthopanax injection is prepared from the following active ingredients: 1.8-5.5mg/ml of total flavonoids, 0.13-0.70mg/ml of syringin, 0.06-0.34mg/ml of eleutheroside E and less than or equal to 800micro g/ml of potassium ions. The acanthopanax injection is low in content of potassium ions, which is reduced from less than or equal to 1000 micro g/ml in the national standard to lower than 800 micro g/ml (or counting at 800micro g/5mg total flavonoids). The injection has the advantages of small abnormal toxicity and high safety. Animal experiments indicate that the tolerated concentration of a study subject is increased from 5mg/ml in the national standard to 18mg/ml. The abnormal toxicity inspection result on the low-toxicity acanthopanax injection provided by the invention is superior to the existing commercially available products.

The invention discloses a quantitative analysis method of six chemical components in a Chinese herbal medicine compound preparation using rhizoma dioscoreae nipponicae and acanthopanax roots as Chinese herbal medicines. The quantitative analysis method comprises the following steps: (1) preparing a mixed control solution; (2) preparing a first test sample solution and a second test sample solution; (3) determining contents, namely, respectively taking the mixed control solution, the first test sample solution and the second test sample solution, performing gradient elution under high performance liquid chromatography detection conditions of using C18 bonded silica gel as a chromatographic column of a filling agent and acetonitrile-formic acid solution as a mobile phase, performing high performance liquid chromatography detection by combining a diode array detector and an evaporative light-scattering detector of detectors to obtain high performance liquid chromatograms of the mixed control solution, the first test sample solution and the second test sample solution, and determining the contents of dioscin, pseudoprotodioscin, protodioscin, chlorogenic acid, syringin and isofraxidin in a test sample by using an external standard method. The quantitative analysis method is simple, convenient and rapid to operate, high in accuracy and excellent in repeatability, and can be used for improving the quality control level of a product.

The method for culturing hairy root and regenerative seedling of saussurea involucrata and producing syringin includes the following steps: placing the green seed leaf of saussurea involucrata germinated seed on the induced comatus seedling culture medium to make culture to obtain comatus bud, placing the comatus bud on the seedling growth culture medium to make single plant culture, placing the single plant on the root-inducing culture medium to make culture to obtain the regenerative seedling whose lower end has root from seedling, cutting explant of seedling with root from seedling, placing the obtained leaf segment, leaf stalk segment and root segment on the preculture culture medium to make dark culture, using root-growing agrobacterium mediation to make conversion, culturing and inducing explant to promote root growth, placing the obtained hairy root on the screening culture medium to make subculture, and making liquid amplification culture of root tip to obtain regenerative seedling, drying hairy root and its regenerative seedling culture, pulverizing, separating by using organic solvent, extracting to obtain its syringin.

The invention belongs to the field of medicine products and particularly relates to a biological membrane preparation for promoting wound healing and a preparation method of the biological membrane preparation. The biological membrane preparation is prepared from components in parts by weight as follows: 0.20-0.75 parts of saccharomycopsis fibuligera polysaccharide extract, 10-25 parts of calciumalginate, 2-6 parts of syringin, 100-150 parts of chitosan and 50-80 parts of silk fibroin. Compared with the prior art, the biological membrane preparation for promoting wound healing has functions of rapid hemostasis and rapid healing; the components are safe and non-irritant and have the effect of repairing scars. The biological membrane preparation is low in cost, good in effect and free of side effects and belongs to natural products.

The invention discloses a fingerprint map and high performance liquid chromatography identification method for a Xuezhitong capsule. The invention firstly discloses an establishing method of a Xuezhitong capsule fingerprint map. The establishing method comprises the following steps: (1) adding a Xuezhitong capsule content in an extraction solvent for extraction to obtain an extracting solution, and purifying the extracting solution to obtain a test sample solution; (2) performing high performance liquid chromatographic analysis on the test sample solution to acquire a high performance liquid chromatogram; and (3) selecting a common peak from chromatographic peaks in the high performance liquid chromatogram to obtain the Xuezhitong capsule fingerprint map. The invention further discloses the Xuezhitong capsule fingerprint map established by the method. The invention also discloses a Xuezhitong capsule high performance liquid chromatography identification method. The method can accurately identify adenosine and syringin in the Xuezhitong capsule and is good in accuracy, stability and repeatability. The fingerprint map and high performance liquid chromatography identification method for the Xuezhitong capsule, established by the invention, has an application prospect in quality control of the Xuezhitong capsule.

The invention provides a ginseng-acanthopanax oral liquid and a production process thereof. The following raw materials are adopted in parts by weight: 1 part of ginseng, 10-20 parts of acanthopanax and 15-40 parts of purified water. The following index system is adopted to strictly control product quality: the content of syringin is more than or equal to 2.0mg/100ml, the pH value is 4.0-6.0, and the soluble solid content is more than or equal to 1.0%. The production process of the ginseng-acanthopanax oral liquid, provided by the invention, omits a step of alcohol precipitation, and the loss of ginseng polysaccharides is avoided, so that good anti-fatigue effect of the oral liquid is guaranteed; and the whole technological process adopts sterile operation, high temperature sterilization and suitable packing material, so that the microbiological safety of a product is guaranteed, the product is stable, no antiseptic substance is required to be added, and a food is safe and reliable.

The invention provides an acanthopanax senticosus detection method; a liquid chromatography method is used for detecting a test sample solution and a reference solution, and liquid chromatography determination conditions comprise that octadecylsilane bonded silica gel is used as a filler; an acetonitrile-water mixture is used as a mobile phase, and an external standard method is used for detecting the content of syringin in a test sample. The detection method has the advantages of smooth baseline and good separating degree, can effectively detect and quantify the effective compound syringin in acanthopanax senticosus.

The invention discloses a preparation method of syringin. The method specifically includes the steps of: (1) drying, crushing, sieving the Chinese herbal material ilex rotunda, and loading the productinto an extraction container; (2) conducting refluxing extraction with a solvent, combining the extracted liquid, and conducting concentration to an appropriate amount; (3) adding an alcohol solventinto the extracted liquid, performing heating to boiling, and conducting decolorization; (4) filtering impurities out of the decolorized solution, adjusting the solution to neutral, and concentratingthe filtrate; and (5) carrying out crystallization refining treatment. The preparation method of syringin disclosed by the invention has the advantages of high syringin content, convenient extraction,environmental protection and cost reduction. At the same time, the method is simple and fast, has high separation efficiency and achieves a good effect.

The present invention relates to recombinant glycosyl transferase UGT72E3/2 gene having an excellent syringin synthesis ability based on remarkable enzyme specificity for sinapyl alcohol, a method for producing a transgenic plant with increased syringin production based on a metabolic process which uses F5H and CHS genes that are involved with the phenylpropanoid biosynthesis pathway and Myb58 gene as a transcription factor for positive regulation of the gene that is involved with lignin biosynthesis pathway, and a plant obtained by the method. According to the present invention, syringin with various pharmaceutical applications can be effectively produced in a large amount in a plant, and thus it is expected to allow the development of an industry relating to agrobiological materials that are highly valuable as foods or pharmaceuticals.

The invention discloses a saussurea involucrata extract. The saussurea involucrata extract contains 0.5-1.2% of syringin, 7-17% of chlorogenic acid and 8-25% of rutin in percentage by weight. The saussurea involucrata extract is obtained according to the following steps: S1, crushing saussurea involucrata, then putting into an extraction tank, extracting with ethanol, and combining the extracts; S2, filtering, cooling and centrifuging the extract to obtain a supernatant; S3, concentrating the supernatant to obtain a concentrate; S4, adding macroporous resin to the concentrate for purification,then eluting with ethanol, fractionally collecting the effluent, concentrating the effluent into thick paste or making the effluent into dry paste powder, thereby obtaining the saussurea involucrataextract. The supernatant is concentrated by using an MVR evaporator, so that the operation is stable, the degree of automation is high, the power consumption is low, and the operating cost is low; inaddition, the process is simple, the practicability is strong, and partial load operation characteristics are excellent; and the saussurea involucrata extract has the functions of absorbing ultraviolet rays, resisting radiation, resisting inflammation, easing pains, resisting ageing and the like in cosmetics.

The invention relates to application of syringin in preparing drug combination for treating cardiovascular and cerebrovascular diseases. The syringin preparation is safe and nontoxic and has strong pharmacological action and good drug effect, thereby indicating bright prospect in drugs; the syringin preparation is simple in preparing process, has no toxic side effects and can be in oral forms such as tablets, pills, capsules and the like, or in injectable forms such as powder-injection, instillations and the like; the drugs prepared by the syringin can evidently lessen the degree and scope of myocardial ischemia caused by the anterior descending branch of canine coronary artery, reduce the myocardial oxygen consumption index, obviously withstand barium chloride induced arrhythmia in rats and reduce the cerebral infarct area of focal cerebral ischemia in rats; the above effects show that the drugs have obvious effects against arrhythmia, myocardial ischemia and cerebral ischemia. The drugs can be clinically used for preventing and treating coronary diseases, angina, stroke and cerebral infarct, etc.

The invention discloses a preparation process and a quality control method of an Eleutherococcus senticosus reference extract, the Eleutherococcus senticosus reference extract is prepared by decocting and extracting Eleutherococcus senticosus decoction pieces as a raw material, in the preparation process, the powder yield and the content of syringin which is an effective substance can be effectively controlled by reasonably controlling the decoction times, the water addition amount, the decoction time and the like, the stability of the radix acanthopanacis senticosi reference extract component is ensured, and the process is stable in operation. The quality control method can realize the stability and reliability of the preparation process of the Eleutherococcus senticosus reference extract.

The invention discloses a method for extracting a syringin compound from tobaccos. The method is characterized by comprising the following steps: preparing a tobacco extract; dissolving the tobacco extract into water, and extracting; eluting an extraction solution with ethanol in a macroporous resin column; collecting eluate, performing reduced pressure concentration, performing capillary sampling thin-layer chromatography, and combining eluate containing the similar components; performing reduced pressure concentration till a solid state to obtain a polar tobacco extract; performing thin-layer chromatography, combining samples with amaranth spots, and performing reduced pressure concentration to obtain a crude extract; uniformly stirring the crude extract and silica gel, separating by using a normal phase silica gel chromatographic column, and eluting; through the chromatographic column, combining fragrance-forming substances according to different Rf values and different polarities to obtain a fine extract; performing normal phase column chromatography on the fine extract, and eluting, segmenting into 5 parts A-E during TLC detection, performing gel column chromatography on a segment B, feeding the segment B into a semi-preparative chromatography system, and separating; under chromatographic conditions, retaining for 14.32 minutes, and separating to obtain the monomer compound.