188 results about "Oleuropein" patented technology

The invention discloses a method for extracting oleuropein from olive leaves, which comprises the following steps of: extracting with water or a mixed solvent of alcohol and water, and then concentrating an extracting solution to separate the extracting solution through three steps in sequence: absorbing through using macroporous resins and eluting with a solvent of the alcohol and the water, absorbing through using decolouration resins, and extracting through using an organic solvent, wherein the macroporous resins have an excellent adsorption effect for the oleuropein and are used for eluting to remove other impurities, and the decolouration resins have an excellent adsorption effect for pigments in the olive extracting solution and are used for absorbing and removing pigments; separating and purifying the oleuropein in the extracting solution step by step; at last, acquiring an oleuropein extract with high content. The content of the oleuropein is more than 40 %, even up to 80-90 %.

The invention discloses a method of using crude extract of olive leaf oleuropein to prepare hydroxytyrosol. Compared with acid hydrolysis and alkali hydrolysis, the enzymolysis process for crude extract of olive leaf oleuropein is mild in method condition, and consumption of acid and alkali catalysts is reduced in the whole process. Hemicellulase is selected from ten enzymes as an enzyme best in oleuropein degrading effect by taking oleuropein degrading rate and hydroxytyrosol content as indexes, optimal process conditions include 60 DEG C of temperature, 5.5 of pH value, 40mg of enzyme amount and 6h of time, obtained HT content is 6.07%, and OE degrading rate is 85.28%.

Beneficial topical compositions for treating scar or aging skin are provided. In one embodiment, the compositions include Bulbine frutescens, Centella asiatica and a phenol derived from olives, such as oleuropein. The composition may be used in the treatment of scars formed during surgery. The composition may also be used cosmeceutically in the treatment of aged skin, and may include phosphatidylserine, vitamins, and other beneficial anti-aging ingredients.

The invention provides an olive leaf extract which comprises any one or a combination of 5%-50% by mass of hydroxytyrosol, Oleuropein and Verbascoside. The invention further provides a preparation method of the olive leaf extract and application in cosmetics. The olive leaf extract has a high anti-aging effect because the olive leaf extract can greatly increase the content of collagen in a corium layer of a human body, so that the elasticity of the skin of the human body is maintained, and wrinkles are inhibited fundamentally. In addition, the olive leaf extract can obviously inhibit generation of free radicals (DPPH) and can serve as a product or an additive to be applied to foods, medicines and skin care products and / or the cosmetics, and the double effects of resisting oxidation and aging are played.

The invention discloses a method for preparing olive leaf extract, belonging to the technical field of plant effective ingredient extraction and separation. The method for extracting and separating oleuropein from olive leaves in the invention has the following steps: extracting the olive leaves by polar solvent, precipitating by flocculant to obtain centrifugal solution and precipitate, extracting the centrifugal solution, further separating by preparative high performance liquid chromatography (HPLC) for acetic ether extractive phase, performing on-line detection by a differential refraction detector, objectively collecting oleuropein product segments, performing reduced pressure concentration on preparation liquid, and carrying out vacuum drying or spray drying to obtain the olive leaf extract in which the oleuropein content is larger than 90%. The invention has the advantages of simple procedures, realization of high purity oleuropein preparation, large preparation amount, high yield and good product quality and is suitable for industrialized production; and the prepared extract has no residual of harmful substances.

The present invention is directed to compositions containing at least a polyphenol and polyethylenglycol, to products such as food, beverages, dietary supplements, feed, pharmaceuticals and personal care products containing such a composition as well as to the use of polyethylenglycol for masking the bitter taste of such polyphenols. The polyphenols are preferably selected from the group consisting of epigallocatechin gallate, resveratrol, hydroxytyrosol, oleuropein, polyphenols present in green tea extracts, catechins, polyphenols present in extracts of red grape skin, polyphenols present in olives and / or olive waste water, and their mixtures.

The invention discloses a method and an apparatus used for preparing an olive leaf extract. The method comprises the steps that: olive leaves are subjected to ultrasonic vacuum boiling extraction by using a mixed solvent; vacuum pump-filtration is carried out, such that a clarified liquid is obtained; fat-soluble impurities are removed through petroleum ether extraction, and a lower-layer clear solution is obtained; the clear solution is subjected to rotary evaporation until no alcohol smell is left in the solvent; the solution is diluted; the diluant is subjected to macroporous resin purification; the obtained solution is subjected to standing for a period of time, such that oleuropein and flavonoid substances are sufficiently absorbed; the oleuropein is eluted by using an ethanol-water mixed solvent with a proper concentration, and a target liquid is collected; the collected target liquid is subjected to dextrane gel purification; standing is carried out, and an ethanol-water solution is used for eluting; a eluant containing effective components is collected; the collected eluant is subjected to rotary evaporation until no alcohol smell is left; and the obtained material is dried, such that the extract is obtained. The oleuropein content of the extract reaches 85-92%, and the total flavonoid content of the extract reaches 25-35%. The method has the advantages of simple operation, short extraction time, high efficiency, high product recovery rate, good quality, and stable quality.

The invention relates to a preparation method of oleuropein, which has the characteristics of convenient operation and less pollution and energy consumption and comprises the following process steps of: taking dries jasminum polyanthum flowers, adding into a CO2 supercritical extractor to obtain an extract by using ethanol as an entrainer, adding water for dissolving, extracting 1-3 times with an equal quantity of ethyl acetate, taking a water layer, adsorbing through an AB-8 macroporous absorbent resin column, eluting with 35-percent ethanol, collecting an eluant accounting for 3-8 times the column volume, recovering the ethanol through decompression, concentrating, refrigerating for 12-36 hours, filtering, taking filtering residues, adding anhydrous ethanol for recrystallization, separating crystals, washing and drying. The oleuropein prepared by adopting the invention is high in product purity and easy for industrialized amplification.

Compositions and methods of use thereof for the treatment of psoriasis and related skin ailments are disclosed. The compositions include topical skin formulations of glucosamine in an emollient base such as moisturizing cream. In addition to glucosamine, the formulations may include keratolytic substances such as coal tar extract or salicylic acid. The formulations may also include glucosamine and antioxidant anti-inflammatory herbal extracts such as oleuropein and berberine in an emollient base.

Beneficial topical compositions for treating scar or aging skin are provided. In one embodiment, the compositions include Bulbine frutescens, Centella asiatica and a phenol derived from olives, such as oleuropein. The composition may be used in the treatment of scars formed during surgery. The composition may also be used cosmeceutically in the treatment of aged skin, and may include phosphatidylserine, vitamins, and other beneficial anti-aging ingredients.

A composition for treating skin pigmentation, a method of forming the composition, and a method of treating skin pigmentation are disclosed. The composition comprises at least one pharmaceutically acceptable additive. The composition further comprises at least one melanin production inhibitor, which may be a depigmentation agent. The melanin production inhibitor is present in an amount effective to inhibit melanin production in human skin cells of a subject that is administered the composition. The melanin production inhibitor is selected from the group consisting of cyclovirobuxine D, lipoic acid, nuciferine, peimisine, oleuropein, Rhodiola algida extract, Rhodiola kirilowii (Regel) Maxim extract, Glycyrrhizae Radix et Rhizoma extract, Cirsii japonici herba extract, Platycladi semen extract, Synotis erythropappa extract, Astragali complanati semen extract, Plantaginis semen extract, Lycopi herba extract, Euryales semen extract, Cuscutae semen extract, Amomi fructus extract, Tara seed extract, Platycladi cacumen extract, Eucommiae cortex extract, Dioscoreae rhizoma extract, Cirsii herba extract, and combinations thereof.

The invention discloses an extraction process for hydroxytyrosol from an olive leaf, belonging to the technical field of extraction of active components from medicinal plants. The extraction process comprises the following steps: crushing the olive leaf into coarse powder, carrying out percolation extraction by using ethanol with a concentration of 80%, subjecting liquid extract to adsorption with D101 resin, then carrying out elution by using ethanol with a concentration of 60% and carrying out concentration and drying on eluate so as to obtain oleuropein with a concentration of 45 to 55%; and hydrolyzing oleuropein with a sodium hydroxide solution, adding acid for neutralization after complete hydrolysis, carrying out precipitation and centrifugation, subjecting obtained centrifugate to adsorption with D101 resin and elution with hot ethanol, concentrating eluate, then repeatedly carrying out extraction with ethyl acetate four times, and carrying out concentration and drying on ethyl acetate-layer liquid so as to obtain hydroxytyrosol with a concentration of 80 to 90%. The extraction process for hydroxytyrosol from the olive leaf is high in the utilization rate of raw materials, and the prepared oleuropein and hydroxytyrosol can greatly meet market demands.