190 results about "Oleuropein" patented technology

Compositions comprising hydroxytyrosol-containing formulations and treatment regiments comprising hydroxytyrosol and / or oleuropein and chemotherapeutic agents are disclosed. Compositions and / or regiments may optionally include the administration of vitamins, minerals, and anti-oxidants. Methods for using these compositions and treatment regimens for treating subjects for diseases, such as a malignancy, and for inducing or enhancing angiogenesis, treating or preventing oxidative stress, for treating or preventing high glucose-induced dysfunction, treating or preventing chemotherapy-induced dysfunction, and for improving cell viability are provided. Various methods for use of the hydroxytyrosol compositions for inhibition of lysine specific demethylase 1 (LSD1) in various cancers are also provided.

The present invention relates to methods and pharmaceutical compositions for treating obesity or obesity-related disorders in a subject suffering from or predisposed to developing obesity or an obesity-related disorder, or for inhibiting the infectivity of HIV, by administering oleuropein, an analogue or derivative thereof, or the major metabolites of oleuropein including oleuropein aglycone, hydroxytyrosol, and elenolic acid or their analogues, or derivatives thereof, an iridoid glycoside, or a secoiridoid glycoside or analogues or derivatives thereof, or any combination of the foregoing including olive leave extract. The invention also relates to methods for screening / diagnosing a subject having, or predisposed to having obesity or a related disorder by measuring the expression profiles of an adipogenic gene selected from PPARγ2, LPL and αP2 gene and gene product, or other adipogenic, lipogenic, or lipolytic genes and gene products in an individual. The invention further provides for screening for novel oleuropein analogues.

The invention discloses a method for extracting oleuropein from olive leaves, which comprises the following steps of: extracting with water or a mixed solvent of alcohol and water, and then concentrating an extracting solution to separate the extracting solution through three steps in sequence: absorbing through using macroporous resins and eluting with a solvent of the alcohol and the water, absorbing through using decolouration resins, and extracting through using an organic solvent, wherein the macroporous resins have an excellent adsorption effect for the oleuropein and are used for eluting to remove other impurities, and the decolouration resins have an excellent adsorption effect for pigments in the olive extracting solution and are used for absorbing and removing pigments; separating and purifying the oleuropein in the extracting solution step by step; at last, acquiring an oleuropein extract with high content. The content of the oleuropein is more than 40 %, even up to 80-90 %.

The present invention provides economical methods for collecting oleuropein aglycon from olive vegetation water, a routine byproduct in the manufacture of olive oil. The methods have the advantage of facilitating the collection of other valuable constituents of olive vegetation water, and furthermore render the olive vegetation water environmentally benign, and thus suitable for routine disposal.

The invention discloses a process method for synchronously preparing oleuropein and olive total flavonoids from olive leaves. Olive leaves serve as raw materials, and the method comprises the following steps of: performing reflux or multiple extraction, series adsorption through macroporous adsorption resin, and gradient desorption; and recovering an organic solvent in desorption solution, and performing spray drying to respectively obtain high-content extracts of the oleuropein and the olive total flavonoids. The invention has the characteristics that: the high-content extracts of the oleuropein and the olive total flavonoids can be synchronously prepared, the production cost is low, the method is environmentally-friendly, and the process is simple.

The invention provides olive-derived vegetation water substantially free of monophenolic compounds (e.g., tyrosol and its derivatives) from olive pits. According to one aspect of the invention, the pits or seeds are removed from the olives prior to pressing. The pitless pulp or meat is then pressed to obtain a liquid-phase mixture including olive oil, vegetation water, and solid by-products. The vegetation water is separated from the rest of the liquid-phase mixture and collected. The vegetation water is useful as a source of oleuropein.

The invention discloses a method of using crude extract of olive leaf oleuropein to prepare hydroxytyrosol. Compared with acid hydrolysis and alkali hydrolysis, the enzymolysis process for crude extract of olive leaf oleuropein is mild in method condition, and consumption of acid and alkali catalysts is reduced in the whole process. Hemicellulase is selected from ten enzymes as an enzyme best in oleuropein degrading effect by taking oleuropein degrading rate and hydroxytyrosol content as indexes, optimal process conditions include 60 DEG C of temperature, 5.5 of pH value, 40mg of enzyme amount and 6h of time, obtained HT content is 6.07%, and OE degrading rate is 85.28%.

The invention discloses a preparation method of an olive leaf extract and the olive leaf extract prepared by the preparation method. An olive leaf extract product of which the oleuropein content is greater than 90% is obtained through the processes of infiltration, enzymatic extraction, separation and drying on olive leaves. The whole method only comprises four steps, the adopted reagents are common non-toxic ingredients, the yield is high, the cost is low, the obtained product can be directly used for preparing medicines, health products and foods, and the method is very suitable for industrial production.

Beneficial topical compositions for treating scar or aging skin are provided. In one embodiment, the compositions include Bulbine frutescens, Centella asiatica and a phenol derived from olives, such as oleuropein. The composition may be used in the treatment of scars formed during surgery. The composition may also be used cosmeceutically in the treatment of aged skin, and may include phosphatidylserine, vitamins, and other beneficial anti-aging ingredients.

A composition containing EPA and EHA, preferably derived from fish oil, in combination with at least one of oleuropein, allicin, and policosanol having anti-inflammatory, anti-thrombotic, immunomodulatory activity as well as activity to lower triglycerides and low density lipoprotein, to increase high density lipoprotein, and to lower both systolic and diastolic blood pressure.

The invention discloses a method for extracting oleuropein from canarium album raeusch fruits by utilizing a subcritical water extraction technology, and belongs to the technical field of separation of active ingredients from natural products. The method comprises the following steps of: performing extraction by taking pre-treated canarium album raeusch fruit flesh as a raw material and taking water as a solvent under the extraction pressure of 5 to 10 MPa and the extraction temperature of 200 to 270 DEG C for 20 to 60 minutes, performing cooling, filtration and concentration, performing separation and purification by using a macroporous resin chromatographic column, and performing vacuum concentration and freeze-drying on eluent to obtain an oleuropein product with the phenol content of over 85 percent. According to the extraction method, the use of an organic solvent is avoided; the method is clean, environment-friendly, simple in equipment, easy to operate, high in extraction speed and high in efficiency, and has broad industrial application prospect; and the product is natural and nontoxic.

The present invention provides a nutritional composition and a pharmaceutical composition to be used for humans or animals comprising as active compound the oleuropein compound or one derivative thereof.

The invention provides an olive leaf extract which comprises any one or a combination of 5%-50% by mass of hydroxytyrosol, Oleuropein and Verbascoside. The invention further provides a preparation method of the olive leaf extract and application in cosmetics. The olive leaf extract has a high anti-aging effect because the olive leaf extract can greatly increase the content of collagen in a corium layer of a human body, so that the elasticity of the skin of the human body is maintained, and wrinkles are inhibited fundamentally. In addition, the olive leaf extract can obviously inhibit generation of free radicals (DPPH) and can serve as a product or an additive to be applied to foods, medicines and skin care products and / or the cosmetics, and the double effects of resisting oxidation and aging are played.

The invention discloses a method for purifying hydroxytyrosol. The method for purifying the hydroxytyrosol comprises the following steps of: dissolving an oleuropein crude product by using deionized water under stirring; passing through an adsorption type macroporous resin chromatographic column, washing with the deionized water, performing elution by using ethanol aqueous solution, and collecting eluent; performing vacuum concentration until no alcohol exists to obtain refined oleuropein aqueous solution; adding an inorganic acid to react; adding sodium hydroxide to adjust the pH value to be 7 to obtain hydroxytyrosol-containing aqueous solution; performing vacuum concentration; performing extraction by using equivalent ethyl acetate; and laminating ester and concentrating to remove the ethyl acetate to obtain faint yellow paste hydroxytyrosol with purity of more than or equal to 80 percent. In the method, the characteristic of one-way conversion from the oleuropein in an olive leaf resource to the hydroxytyrosol is completely utilized, so that the conversion rate is high, the current situation that the oleuropein component is blindly separated in the purification process of the oleuropein is changed, the oleuropein is converted into the hydroxytyrosol with high economic value, economic benefit is improved, and difficulty in preparation of high-content hydroxytyrosol is greatly reduced.

Beneficial topical compositions for treating scar or aging skin are provided. In one embodiment, the compositions include Bulbine frutescens, Centella asiatica and a phenol derived from olives, such as oleuropein. The composition may be used in the treatment of scars formed during surgery. The composition may also be used cosmeceutically in the treatment of aged skin, and may include phosphatidylserine, vitamins, and other beneficial anti-aging ingredients.

The invention discloses a method for preparing olive leaf extract, belonging to the technical field of plant effective ingredient extraction and separation. The method for extracting and separating oleuropein from olive leaves in the invention has the following steps: extracting the olive leaves by polar solvent, precipitating by flocculant to obtain centrifugal solution and precipitate, extracting the centrifugal solution, further separating by preparative high performance liquid chromatography (HPLC) for acetic ether extractive phase, performing on-line detection by a differential refraction detector, objectively collecting oleuropein product segments, performing reduced pressure concentration on preparation liquid, and carrying out vacuum drying or spray drying to obtain the olive leaf extract in which the oleuropein content is larger than 90%. The invention has the advantages of simple procedures, realization of high purity oleuropein preparation, large preparation amount, high yield and good product quality and is suitable for industrialized production; and the prepared extract has no residual of harmful substances.

The present invention is directed to compositions containing at least a polyphenol and polyethylenglycol, to products such as food, beverages, dietary supplements, feed, pharmaceuticals and personal care products containing such a composition as well as to the use of polyethylenglycol for masking the bitter taste of such polyphenols. The polyphenols are preferably selected from the group consisting of epigallocatechin gallate, resveratrol, hydroxytyrosol, oleuropein, polyphenols present in green tea extracts, catechins, polyphenols present in extracts of red grape skin, polyphenols present in olives and / or olive waste water, and their mixtures.

The invention discloses a preparation method of long-circulation lipidosome of an olive polyphenol extract rich in hydroxytyrosol and verbascoside. Olive leaf is taken as a raw material, and is prepared into an olive polyphenol extract by extracting with 30 to 50 percent of ethanol, separating with a ceramic membrane and an ultrafiltration membrane and concentrating with a nanofiltration membrane; 90 percent of the hydroxytyrosol, 10 to 40 percent of an oleuropein extract and 90 percent of the verbascoside are compounded to prepare the olive polyphenol extract rich in the hydroxytyrosol and the verbascoside; the hydroxytyrosol and verbascoside polyphenol as model materials, lecithin, cholesterol, a Tween-80 surfactant and the like are prepared into the corresponding lipidosome. The lipidosome has the advantages that the bioavailability of the olive polyphenol extract is remarkably increased, and high encapsulation efficiency, uniform particle size distribution, good stability, slow release property, oxidation resistance and an antibacterial effect are achieved. The preparation method is simple and feasible, equipment is simple, and the application of the lipidosome in the medicine field can be widened.

The invention discloses a method and an apparatus used for preparing an olive leaf extract. The method comprises the steps that: olive leaves are subjected to ultrasonic vacuum boiling extraction by using a mixed solvent; vacuum pump-filtration is carried out, such that a clarified liquid is obtained; fat-soluble impurities are removed through petroleum ether extraction, and a lower-layer clear solution is obtained; the clear solution is subjected to rotary evaporation until no alcohol smell is left in the solvent; the solution is diluted; the diluant is subjected to macroporous resin purification; the obtained solution is subjected to standing for a period of time, such that oleuropein and flavonoid substances are sufficiently absorbed; the oleuropein is eluted by using an ethanol-water mixed solvent with a proper concentration, and a target liquid is collected; the collected target liquid is subjected to dextrane gel purification; standing is carried out, and an ethanol-water solution is used for eluting; a eluant containing effective components is collected; the collected eluant is subjected to rotary evaporation until no alcohol smell is left; and the obtained material is dried, such that the extract is obtained. The oleuropein content of the extract reaches 85-92%, and the total flavonoid content of the extract reaches 25-35%. The method has the advantages of simple operation, short extraction time, high efficiency, high product recovery rate, good quality, and stable quality.

The invention relates to a preparation method of oleuropein, which has the characteristics of convenient operation and less pollution and energy consumption and comprises the following process steps of: taking dries jasminum polyanthum flowers, adding into a CO2 supercritical extractor to obtain an extract by using ethanol as an entrainer, adding water for dissolving, extracting 1-3 times with an equal quantity of ethyl acetate, taking a water layer, adsorbing through an AB-8 macroporous absorbent resin column, eluting with 35-percent ethanol, collecting an eluant accounting for 3-8 times the column volume, recovering the ethanol through decompression, concentrating, refrigerating for 12-36 hours, filtering, taking filtering residues, adding anhydrous ethanol for recrystallization, separating crystals, washing and drying. The oleuropein prepared by adopting the invention is high in product purity and easy for industrialized amplification.

Compositions and methods of use thereof for the treatment of psoriasis and related skin ailments are disclosed. The compositions include topical skin formulations of glucosamine in an emollient base such as moisturizing cream. In addition to glucosamine, the formulations may include keratolytic substances such as coal tar extract or salicylic acid. The formulations may also include glucosamine and antioxidant anti-inflammatory herbal extracts such as oleuropein and berberine in an emollient base.

The invention discloses a natural cleanser. The natural cleanser comprises raw materials in parts by weight as follows: 5-10 parts of a goldthread extract, 5-20 parts of a coconut oil extract, 5-15 parts of an ephedra extract, 3-7 parts of a liquorice root extract, 0.5-1 part of a ginger extraction solution, 0.2-1 part of an aloe extraction solution, 0.4-1 part of oleuropein, 3-5 parts of a lemon enzyme extract and the balance of purified water. The invention further provides a preparation technology of the natural cleanser. The cleanser is environment-friendly, adopts natural and non-toxic raw materials, contains no petrochemical raw materials, phosphorus, sulfur, arsenic and other heavy metals, belongs to a non-toxic and non-irritating product and can be directly degraded into harmless substances in the nature, and a waste liquid after cleaning meets the environmental protection requirement and can be directly discharged; the natural cleanser is wide in application range, high in dirt removal capability and safe and reliable to use, has sterilizing and antibacterial functions, has the effect of protecting skin and cannot endanger the human health.

Beneficial topical compositions for treating scar or aging skin are provided. In one embodiment, the compositions include Bulbine frutescens, Centella asiatica and a phenol derived from olives, such as oleuropein. The composition may be used in the treatment of scars formed during surgery. The composition may also be used cosmeceutically in the treatment of aged skin, and may include phosphatidylserine, vitamins, and other beneficial anti-aging ingredients.

The present invention relates to use of a composition comprising oleuropein and / or hydroxytyrosol for maintenance joint health or prevention or treatment of joint disorders. In particular, the invention relates to oleuropein for use to prevent or treat cartilage breakdown.

A composition for treating skin pigmentation, a method of forming the composition, and a method of treating skin pigmentation are disclosed. The composition comprises at least one pharmaceutically acceptable additive. The composition further comprises at least one melanin production inhibitor, which may be a depigmentation agent. The melanin production inhibitor is present in an amount effective to inhibit melanin production in human skin cells of a subject that is administered the composition. The melanin production inhibitor is selected from the group consisting of cyclovirobuxine D, lipoic acid, nuciferine, peimisine, oleuropein, Rhodiola algida extract, Rhodiola kirilowii (Regel) Maxim extract, Glycyrrhizae Radix et Rhizoma extract, Cirsii japonici herba extract, Platycladi semen extract, Synotis erythropappa extract, Astragali complanati semen extract, Plantaginis semen extract, Lycopi herba extract, Euryales semen extract, Cuscutae semen extract, Amomi fructus extract, Tara seed extract, Platycladi cacumen extract, Eucommiae cortex extract, Dioscoreae rhizoma extract, Cirsii herba extract, and combinations thereof.

The invention relates to a method of preparing a wide range of hydroxytyrosol esters. According to the invention, the compounds are synthesised by reacting synthetic hydroxytyrosol or natural hydroxytyrosol compounds, oleuropein or oleuropein aglycones (which are found in olive oil, vegetable water, olive marc or olive leaves) with an acylating agent which is a compound containing at least one acylic group R, wherein R is H, an alkyl radical with between 1 and 31 linear, branched or cyclic carbon atoms, an alkenyl radical with up to 31 linear, branched or cyclic carbon atoms or an aryl group. The invention also relates to the esters prepared with the inventive method, which can be used as an additive in food and cosmetic products as well as in pharmaceutical preparations.

A medicament for treating skin is described. It comprises essentially of bulbine frutescens, centella asiatica and a phenol derived from olive oil preferably oleuropein. The medicament may be used in the treatment of scars formed during surgery. It may also be used cosmeceutically in the treatment of aged skin where it may be used with phosphatidylserine. The medicament can also act in conjunction with anti-acne treatment to diminish scarring from the acne condition.

The invention discloses an extraction process for hydroxytyrosol from an olive leaf, belonging to the technical field of extraction of active components from medicinal plants. The extraction process comprises the following steps: crushing the olive leaf into coarse powder, carrying out percolation extraction by using ethanol with a concentration of 80%, subjecting liquid extract to adsorption with D101 resin, then carrying out elution by using ethanol with a concentration of 60% and carrying out concentration and drying on eluate so as to obtain oleuropein with a concentration of 45 to 55%; and hydrolyzing oleuropein with a sodium hydroxide solution, adding acid for neutralization after complete hydrolysis, carrying out precipitation and centrifugation, subjecting obtained centrifugate to adsorption with D101 resin and elution with hot ethanol, concentrating eluate, then repeatedly carrying out extraction with ethyl acetate four times, and carrying out concentration and drying on ethyl acetate-layer liquid so as to obtain hydroxytyrosol with a concentration of 80 to 90%. The extraction process for hydroxytyrosol from the olive leaf is high in the utilization rate of raw materials, and the prepared oleuropein and hydroxytyrosol can greatly meet market demands.