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Olive leaf extract and cosmetics containing same

A technology of olive leaf extract and cosmetics, applied in the field of chemistry, can solve the problems of insufficient research on the mechanism of action and few applications

Active Publication Date: 2016-10-12
SHANGHAI INOHERB COSMETIC +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, its application in cosmetics is still relatively small, and the research on its mechanism of action is not thorough enough.

Method used

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  • Olive leaf extract and cosmetics containing same
  • Olive leaf extract and cosmetics containing same
  • Olive leaf extract and cosmetics containing same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1-4

[0071] Wash the freshly picked olive leaves twice with clean water, inject chlorine dioxide with a concentration of 100ppm into the clean water, wash and sterilize the olive leaves, and dry the washed olive leaves with hot air at 65°C to obtain a water content of 5 % less dry olive leaves, and the prepared dry olive leaves are pulverized and set aside.

[0072] Take 10 kg of crushed dry olive leaves, add a certain volume of 50% aqueous methanol as a solvent, and extract 3 times at 65°C and 1.01MPa for 2 hours each time. After the extraction is completed, mix the extracts each time to obtain The extracts were mixed, and the mixed extracts were rotatively evaporated at 5kPa and slightly boiling for 0.5h to remove methanol therein to obtain a concentrated solution.

[0073] Use 1M hydrochloric acid to adjust the concentrated solution to pH = 4, use the concentrated solution to purify by glass column chromatography, and then use a mixed solution with a volume ratio of methanol:eth...

Embodiment 5-13

[0079] Wash the freshly picked olive leaves twice with clean water, inject chlorine dioxide with a concentration of 100ppm into the clean water, wash and sterilize the olive leaves, and dry the washed olive leaves with hot air at 65°C to obtain a water content of 5 % less dry olive leaves, and the prepared dry olive leaves are pulverized and set aside.

[0080] Take 10 kg of crushed dry olive leaves, add 10 times the volume of solvent to it, and extract 3 times at 110°C and 1.5 MPa, each time for 2 hours. After the extraction is completed, mix the extracts each time to obtain a mixed extract. The mixed extracts were rotary evaporated for 0.5 h under 5kPa and slight boiling conditions to obtain a concentrated solution.

[0081] Use 0.8M sulfuric acid to adjust the pH value of the concentrated solution to 3, and use D-101 macroporous adsorption resin to absorb the concentrated solution at 40°C for 2 hours, the adsorption is saturated, and then use different concentrations of eth...

Embodiment 14-18

[0088] Wash the freshly picked olive leaves twice with clean water, inject chlorine dioxide with a concentration of 100ppm into the clean water, wash and sterilize the olive leaves, and dry the washed olive leaves with hot air at 65°C to obtain a water content of 5 % less dry olive leaves, and the prepared dry olive leaves are pulverized and set aside.

[0089] Take 10 kg of crushed dry olive leaves, add 10 times the volume of deionized water as a solvent, extract 3 times at 65°C under different pressures, each time for 2 hours, after the extraction is completed, mix the extracts each time to obtain a mixed The extract, and the mixed extract were rotatively evaporated for 0.5h under 5kPa and micro-boiling conditions to remove methanol therein to obtain a concentrated solution.

[0090] Use 0.8M sulfuric acid to adjust the pH value of the concentrate to 3, purify the concentrate using glass column chromatography, and then use a mixed solution with a volume ratio of methanol:eth...

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Abstract

The invention provides an olive leaf extract which comprises any one or a combination of 5%-50% by mass of hydroxytyrosol, Oleuropein and Verbascoside. The invention further provides a preparation method of the olive leaf extract and application in cosmetics. The olive leaf extract has a high anti-aging effect because the olive leaf extract can greatly increase the content of collagen in a corium layer of a human body, so that the elasticity of the skin of the human body is maintained, and wrinkles are inhibited fundamentally. In addition, the olive leaf extract can obviously inhibit generation of free radicals (DPPH) and can serve as a product or an additive to be applied to foods, medicines and skin care products and / or the cosmetics, and the double effects of resisting oxidation and aging are played.

Description

technical field [0001] The invention belongs to the field of chemistry, and relates to an extract and cosmetics containing the extract, in particular to an olive leaf extract and cosmetics containing the olive leaf extract. Background technique [0002] Large doses of ultraviolet radiation can cause irreversible DNA damage, resulting in direct necrosis of cells, and even canceration; while long-term exposure to small doses of ultraviolet radiation can induce nuclear chromosome breakage, mtDNA mutation and oxidative stress damage, making intracellular reactive oxygen species (ROS ), other oxidative stress markers, etc., are elevated, thereby initiating cell damage and aging processes. [0003] Aging refers to a process in which the functions of various organs of the body are generally and gradually reduced. With the increase of age, aging is inevitable, but good living habits and health care measures can effectively delay aging. At present, there are endless products aimed a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/97A61K8/60A61K8/34A61Q19/00
CPCA61K8/347A61K8/602A61K8/97A61K2800/10A61Q19/00
Inventor 洪民华刘丹赵兆洪奇吕智卢艳花何浩
Owner SHANGHAI INOHERB COSMETIC
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